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The map label for this gene is pilT [H]

Identifier: 66044758

GI number: 66044758

Start: 1697514

End: 1698446

Strand: Reverse

Name: pilT [H]

Synonym: Psyr_1511

Alternate gene names: 66044758

Gene position: 1698446-1697514 (Counterclockwise)

Preceding gene: 66044759

Following gene: 66044757

Centisome position: 27.87

GC content: 55.31

Gene sequence:

>933_bases
ATGAACCCAATTATCGACACGCCTTTTAGTGACCTCTACTTGGGGCCGGATTTTACGGACGTAAAAGGCCTGGATGGCCA
GAGCGGACGTGTCGAGGCTCCGGAAAACTGGAAAGAAAGCGCCCTGCTATTACGGGAGGAATGCGCAAAGCATTTTGAAG
TGCATCAGGATCCTGAGTTCTCGTTGATCAAAGAAGGAACGGTGCTTCGCGTCACTCAGATACCCGATGCCTTTGGCCAG
GCCGTGTTCGTACTGCGCAGATCAAATGCGCAGGTCCGGCAGTTCGACCAATTGGGTTTTCCTGAAGACCTTGTCGAATC
GCTGCTTACGAAGCAGGTGCGCGGCCTGGTGATTTTTTGCGGCGAGATTTGCACCGGTAAGACGTCAAGCGCAGCTTCGC
TGATCATCGCCAGGCTAAATGCGTGCGGTGGAATCGCGTTGGCTGCAGAGGATCCAGCTGAGACAGATTTGAACGGTCGT
CACGGACCTGGTCGTTGCATCCAGATGCAGGTTTCGAGACGCAATGGCGGGTATGAGGAGGCTTTGATGAGAGGCCTCCG
CTCTGGCGCGGACATGATGCTTATCGGTGAGATCAGAGACACCCCCACGGCAGTTCAGGCAGTGCGAGCGGCAATCAATG
GCCTGTTCATCATCACCACAGTTCACGCGGGAACAGTACCGCAGGCGATCGAGCGAGTAGCGACTCTTGCGGAGCCGGCC
ATCAGCAACGTCCGATCAATTCTGTCACAGGGTTTGGTAGCTGTAGCTGCGCTTGCCCTCGAAGGTGAAGGCGAAATGCG
ACATCTGAAGATCCAGAGCCTTTCACTGACTGGACCTGACGGCCCTGGTATTCGCGAGAAGATCCGTTCGAATCAGATCG
CGATGCTTAGCCAGGATGTGGATCAACAATCGAACAGGAGCCTCTGGGGATGA

Upstream 100 bases:

>100_bases
TCAATGGCGGCACAGCTGTGAACGGCGAGGTGAGTTCAGCTGCTGCCACCAGTGGCTGCTCTACTGATAGCAACACGTTG
GCTTGGACGGCCTACTGAGC

Downstream 100 bases:

>100_bases
ATGTTCTCAAGCATGGCCAGACCAGAAAGAATGGTTTTGTGGTGATCGAGCTGCTCTTCGGCCTGATCATTTTCGCCATT
GCGTCGGCCATAGGCGTCTC

Product: type II secretion system protein E

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 310; Mature: 310

Protein sequence:

>310_residues
MNPIIDTPFSDLYLGPDFTDVKGLDGQSGRVEAPENWKESALLLREECAKHFEVHQDPEFSLIKEGTVLRVTQIPDAFGQ
AVFVLRRSNAQVRQFDQLGFPEDLVESLLTKQVRGLVIFCGEICTGKTSSAASLIIARLNACGGIALAAEDPAETDLNGR
HGPGRCIQMQVSRRNGGYEEALMRGLRSGADMMLIGEIRDTPTAVQAVRAAINGLFIITTVHAGTVPQAIERVATLAEPA
ISNVRSILSQGLVAVAALALEGEGEMRHLKIQSLSLTGPDGPGIREKIRSNQIAMLSQDVDQQSNRSLWG

Sequences:

>Translated_310_residues
MNPIIDTPFSDLYLGPDFTDVKGLDGQSGRVEAPENWKESALLLREECAKHFEVHQDPEFSLIKEGTVLRVTQIPDAFGQ
AVFVLRRSNAQVRQFDQLGFPEDLVESLLTKQVRGLVIFCGEICTGKTSSAASLIIARLNACGGIALAAEDPAETDLNGR
HGPGRCIQMQVSRRNGGYEEALMRGLRSGADMMLIGEIRDTPTAVQAVRAAINGLFIITTVHAGTVPQAIERVATLAEPA
ISNVRSILSQGLVAVAALALEGEGEMRHLKIQSLSLTGPDGPGIREKIRSNQIAMLSQDVDQQSNRSLWG
>Mature_310_residues
MNPIIDTPFSDLYLGPDFTDVKGLDGQSGRVEAPENWKESALLLREECAKHFEVHQDPEFSLIKEGTVLRVTQIPDAFGQ
AVFVLRRSNAQVRQFDQLGFPEDLVESLLTKQVRGLVIFCGEICTGKTSSAASLIIARLNACGGIALAAEDPAETDLNGR
HGPGRCIQMQVSRRNGGYEEALMRGLRSGADMMLIGEIRDTPTAVQAVRAAINGLFIITTVHAGTVPQAIERVATLAEPA
ISNVRSILSQGLVAVAALALEGEGEMRHLKIQSLSLTGPDGPGIREKIRSNQIAMLSQDVDQQSNRSLWG

Specific function: May be involved in pilus retraction [H]

COG id: COG5008

COG function: function code NU; Tfp pilus assembly protein, ATPase PilU

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=192, Percent_Identity=29.1666666666667, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI87082188, Length=208, Percent_Identity=28.8461538461538, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR006321
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 33497; Mature: 33497

Theoretical pI: Translated: 5.07; Mature: 5.07

Prosite motif: PS00070 ALDEHYDE_DEHYDR_CYS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPIIDTPFSDLYLGPDFTDVKGLDGQSGRVEAPENWKESALLLREECAKHFEVHQDPEF
CCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SLIKEGTVLRVTQIPDAFGQAVFVLRRSNAQVRQFDQLGFPEDLVESLLTKQVRGLVIFC
EEECCCCEEEEECCCHHHHHEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCEEEEE
GEICTGKTSSAASLIIARLNACGGIALAAEDPAETDLNGRHGPGRCIQMQVSRRNGGYEE
CCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHH
ALMRGLRSGADMMLIGEIRDTPTAVQAVRAAINGLFIITTVHAGTVPQAIERVATLAEPA
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHH
ISNVRSILSQGLVAVAALALEGEGEMRHLKIQSLSLTGPDGPGIREKIRSNQIAMLSQDV
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHCCCCHHHHHHHH
DQQSNRSLWG
HHHCCCCCCC
>Mature Secondary Structure
MNPIIDTPFSDLYLGPDFTDVKGLDGQSGRVEAPENWKESALLLREECAKHFEVHQDPEF
CCCCCCCCCHHEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCC
SLIKEGTVLRVTQIPDAFGQAVFVLRRSNAQVRQFDQLGFPEDLVESLLTKQVRGLVIFC
EEECCCCEEEEECCCHHHHHEEEEEECCCCHHHHHHHCCCCHHHHHHHHHHHHHCEEEEE
GEICTGKTSSAASLIIARLNACGGIALAAEDPAETDLNGRHGPGRCIQMQVSRRNGGYEE
CCCCCCCCCHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCCCCEEEEEEECCCCCHHH
ALMRGLRSGADMMLIGEIRDTPTAVQAVRAAINGLFIITTVHAGTVPQAIERVATLAEPA
HHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHH
ISNVRSILSQGLVAVAALALEGEGEMRHLKIQSLSLTGPDGPGIREKIRSNQIAMLSQDV
HHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEEEEECCCCCCCHHHHHCCCCHHHHHHHH
DQQSNRSLWG
HHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1676385; 10984043 [H]