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The map label for this gene is arsB [H]

Identifier: 66044750

GI number: 66044750

Start: 1690145

End: 1691434

Strand: Reverse

Name: arsB [H]

Synonym: Psyr_1503

Alternate gene names: 66044750

Gene position: 1691434-1690145 (Counterclockwise)

Preceding gene: 66044751

Following gene: 66044749

Centisome position: 27.76

GC content: 57.05

Gene sequence:

>1290_bases
ATGCTTACTGCGGCTGCAATTTTTGTATTCACATTGGTACTGGTGATCTGGCAGCCCAAGGGCTTGGGCGTCGGATGGAG
CGCAGCGGCAGGAGCCATTCTTGCTCTGCTAACTGGCGTTGTCAGCCTGAGTGACGTACCTACCGTATGGCACATTGTAT
GGAACGCCACCGCTACCTTTATTGCCGTCATTATCATTAGCCTGTTGCTCGATGAAGCCGGCTTTTTCGAATGGGCTGCG
CTTCATGTGGCGCGCTGGGGAAAGGGAAGTGGAGGACGATTATTTGCGCTGATGATCCTCTTAGGTGCTGCCGTTTCGGC
TCTATTTGCCAATGACGGCGCAGCGTTGATCCTAACGCCTATCGTAATTGCCATGCTGAACGCTTTGCGCTTTAGCCCTG
CCTCTACTTTAGCGTTCGTCATGGCTGCAGGTTTCATCGCAGACACTGCGAGCTTGCCCCTGGTGGTCTCCAACCTGGTC
AACATCGTCTCTGCTGACTTCTTCAACGTAGGCTTCGGGGAGTACGCCTCGATCATGTGGCCGGTGAACCTGATCAGCGT
GGCAGCCACGTTGTTGATGCTTTGGCTGTTCTACAAAAAAGACATTCCCGCACGGTACGTGCCTGAGCAGCTGGACGTGC
CCAGGCGCGCCATTCGCGACCGTGCGACCTTTATTGCGGGCTGGTGGGTACTGGTGCTACTGCTTGTGGGCTTGTTCGCC
TTAGAGCCGCTGGGTATCCCCATCAGCGCTGTGGCAGCGGCGTGCGCGCTTATCCTCCTGATCGTCGCTGGCAGGGGCCG
CGTCATCTCGACCCGAAACGTGATCAAGCATGCCCCGTGGCAGGTCGTGGTCTTCTCCTTGGGAATGTACCTGGTCGTGT
ATGGGTTGCGTAACGCCGGCCTGACAGATGCCTTTACCGCGTTACTCAATGCGCTGGCTGAGCAAGGCCTGTGGGCAGCT
ACCCTGGGTACTGGCCTAGCCGCCGCCCTGCTGTCCTCGGTCATGAACAACATGCCTAGTGTTTTGATCGGAGCCCTTTC
TATTCAGGCTAGCAATGCCTCGGGCATCGTGCACCAGGGCATGGTGTACGCCAATGTCATCGGCTGTAATCTGGGCCCAA
AAATCACCCCTATTGGTAGCCTGGCTACCCTGCTGTGGCTGCATGTCCTTGCGCAGAAAGACATCCGCATTACCTGGGGT
TATTACTTCAAGGTCGGCAGCCTCCTGACCCTTCCCATCCTGCTCGTCACCTTGGCCGCATTGGCCCTGCGGCTGAGTCT
TCACGCTTAG

Upstream 100 bases:

>100_bases
TCATGCCTGGGTTGATCAAGACTCCAATCGGCTCGAATCCATGCAGGACCGTCCTGTTCGCCAAGCTGCTTGCTGCTGAC
CTTCTGACGAGAGTTTCGTG

Downstream 100 bases:

>100_bases
GATCAATAATGAAAGTTCTATTTATGTGTACCGCCAATAGCTGCCGGAGCGTGCTCTCTGAAGGCTTGTTCAACCACCTT
GCGCCTGACGGAATGGTGGG

Product: arsenical pump membrane protein

Products: ADP; phosphate; arsenite [Periplasm] [C]

Alternate protein names: Arsenic efflux pump protein [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MLTAAAIFVFTLVLVIWQPKGLGVGWSAAAGAILALLTGVVSLSDVPTVWHIVWNATATFIAVIIISLLLDEAGFFEWAA
LHVARWGKGSGGRLFALMILLGAAVSALFANDGAALILTPIVIAMLNALRFSPASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADFFNVGFGEYASIMWPVNLISVAATLLMLWLFYKKDIPARYVPEQLDVPRRAIRDRATFIAGWWVLVLLLVGLFA
LEPLGIPISAVAAACALILLIVAGRGRVISTRNVIKHAPWQVVVFSLGMYLVVYGLRNAGLTDAFTALLNALAEQGLWAA
TLGTGLAAALLSSVMNNMPSVLIGALSIQASNASGIVHQGMVYANVIGCNLGPKITPIGSLATLLWLHVLAQKDIRITWG
YYFKVGSLLTLPILLVTLAALALRLSLHA

Sequences:

>Translated_429_residues
MLTAAAIFVFTLVLVIWQPKGLGVGWSAAAGAILALLTGVVSLSDVPTVWHIVWNATATFIAVIIISLLLDEAGFFEWAA
LHVARWGKGSGGRLFALMILLGAAVSALFANDGAALILTPIVIAMLNALRFSPASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADFFNVGFGEYASIMWPVNLISVAATLLMLWLFYKKDIPARYVPEQLDVPRRAIRDRATFIAGWWVLVLLLVGLFA
LEPLGIPISAVAAACALILLIVAGRGRVISTRNVIKHAPWQVVVFSLGMYLVVYGLRNAGLTDAFTALLNALAEQGLWAA
TLGTGLAAALLSSVMNNMPSVLIGALSIQASNASGIVHQGMVYANVIGCNLGPKITPIGSLATLLWLHVLAQKDIRITWG
YYFKVGSLLTLPILLVTLAALALRLSLHA
>Mature_429_residues
MLTAAAIFVFTLVLVIWQPKGLGVGWSAAAGAILALLTGVVSLSDVPTVWHIVWNATATFIAVIIISLLLDEAGFFEWAA
LHVARWGKGSGGRLFALMILLGAAVSALFANDGAALILTPIVIAMLNALRFSPASTLAFVMAAGFIADTASLPLVVSNLV
NIVSADFFNVGFGEYASIMWPVNLISVAATLLMLWLFYKKDIPARYVPEQLDVPRRAIRDRATFIAGWWVLVLLLVGLFA
LEPLGIPISAVAAACALILLIVAGRGRVISTRNVIKHAPWQVVVFSLGMYLVVYGLRNAGLTDAFTALLNALAEQGLWAA
TLGTGLAAALLSSVMNNMPSVLIGALSIQASNASGIVHQGMVYANVIGCNLGPKITPIGSLATLLWLHVLAQKDIRITWG
YYFKVGSLLTLPILLVTLAALALRLSLHA

Specific function: Involved in arsenical resistance. Thought to form the channel of an arsenite pump [H]

COG id: COG1055

COG function: function code P; Na+/H+ antiporter NhaD and related arsenite permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ArsB family [H]

Homologues:

Organism=Escherichia coli, GI87082277, Length=427, Percent_Identity=71.4285714285714, Blast_Score=582, Evalue=1e-167,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000802 [H]

Pfam domain/function: PF02040 ArsB [H]

EC number: NA

Molecular weight: Translated: 45444; Mature: 45444

Theoretical pI: Translated: 9.75; Mature: 9.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTAAAIFVFTLVLVIWQPKGLGVGWSAAAGAILALLTGVVSLSDVPTVWHIVWNATATF
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IAVIIISLLLDEAGFFEWAALHVARWGKGSGGRLFALMILLGAAVSALFANDGAALILTP
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLNALRFSPASTLAFVMAAGFIADTASLPLVVSNLVNIVSADFFNVGFGEYASIMW
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PVNLISVAATLLMLWLFYKKDIPARYVPEQLDVPRRAIRDRATFIAGWWVLVLLLVGLFA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEPLGIPISAVAAACALILLIVAGRGRVISTRNVIKHAPWQVVVFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LTDAFTALLNALAEQGLWAATLGTGLAAALLSSVMNNMPSVLIGALSIQASNASGIVHQG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHC
MVYANVIGCNLGPKITPIGSLATLLWLHVLAQKDIRITWGYYFKVGSLLTLPILLVTLAA
HHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LALRLSLHA
HHHHHHCCC
>Mature Secondary Structure
MLTAAAIFVFTLVLVIWQPKGLGVGWSAAAGAILALLTGVVSLSDVPTVWHIVWNATATF
CCHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IAVIIISLLLDEAGFFEWAALHVARWGKGSGGRLFALMILLGAAVSALFANDGAALILTP
HHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHH
IVIAMLNALRFSPASTLAFVMAAGFIADTASLPLVVSNLVNIVSADFFNVGFGEYASIMW
HHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCCHHHHHHHH
PVNLISVAATLLMLWLFYKKDIPARYVPEQLDVPRRAIRDRATFIAGWWVLVLLLVGLFA
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHH
LEPLGIPISAVAAACALILLIVAGRGRVISTRNVIKHAPWQVVVFSLGMYLVVYGLRNAG
HCCCCCCHHHHHHHHHHHHHHHCCCCCEEEHHHHHHHCCHHHHHHHHHHHHHHHHHCCCC
LTDAFTALLNALAEQGLWAATLGTGLAAALLSSVMNNMPSVLIGALSIQASNASGIVHQG
HHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHEECCCCCCHHHHC
MVYANVIGCNLGPKITPIGSLATLLWLHVLAQKDIRITWGYYFKVGSLLTLPILLVTLAA
HHHHHEEECCCCCCCCCHHHHHHHHHHHHHHCCCEEEEEHHHHHHHHHHHHHHHHHHHHH
LALRLSLHA
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; arsenite [Cytoplasm]; H2O [C]

Specific reaction: ATP + arsenite [Cytoplasm] + H2O = ADP + phosphate + arsenite [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9006011 [H]