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The map label for this gene is ruvC [H]

Identifier: 66044656

GI number: 66044656

Start: 1594950

End: 1595474

Strand: Direct

Name: ruvC [H]

Synonym: Psyr_1408

Alternate gene names: 66044656

Gene position: 1594950-1595474 (Clockwise)

Preceding gene: 66044655

Following gene: 66044657

Centisome position: 26.17

GC content: 60.95

Gene sequence:

>525_bases
ATGACTCTTATTCTTGGTATCGATCCCGGTTCGCGAATCACCGGCTATGGCGTGGTACGCGACACCGGGCGCGGCTGTGT
CTATGTCGCGTCGGGCTGCATCCGCACCGGCAGCGGCGAGCTGCATGAGCGTTTGCAGATCGTTTATCGCGGTGTGCGGG
AAGTCATCAAGACCTACGAACCAGTGACGATGGGCATCGAAAAGGTTTTCATGGCGCGTAACGCCGATTCGGCATTGAAG
CTGGGGCAGGCCAGGGGCGCAGCCATCGTGGCGGGTGCCGAAGAGGCGCTGGAAATTGCCGAATACACCGCAACCCAGGT
CAAGCAGGCAGTTGCAGGAACCGGCGGTGCCAATAAGGAACAGGTGATGATGATGGTCATGCACCTGCTCAAGCTGACCC
AGAAGCCGCAAATCGATGCGTCCGATGCCCTTGCCATCGCCCTGTGCCACGCGCATACGCGTTCCAGTCTGATTCCTCAC
GGCTTGGGCACTGCGCGCAGTCGCGGCGGCCGCTTGCGGCTCTAG

Upstream 100 bases:

>100_bases
GGGGGAACGAAGCTGTGAGCAACGCGCAGCTATCGGCCCCCGGCTTCTGCCTTTATGCTGCACCTATTGTTTTGCGTGAC
TTCCTCAAGCTGCAGGCGTT

Downstream 100 bases:

>100_bases
AGGCATTATATGTCCGCTTATTTTCTATGATGACGGTGTTCAACTTCTTCGCGCGAGCGACAGGCAACACGAAAGGATCT
GAAACGTGATTGGACGTTTA

Product: Holliday junction resolvase

Products: NA

Alternate protein names: Holliday junction nuclease ruvC; Holliday junction resolvase ruvC [H]

Number of amino acids: Translated: 174; Mature: 173

Protein sequence:

>174_residues
MTLILGIDPGSRITGYGVVRDTGRGCVYVASGCIRTGSGELHERLQIVYRGVREVIKTYEPVTMGIEKVFMARNADSALK
LGQARGAAIVAGAEEALEIAEYTATQVKQAVAGTGGANKEQVMMMVMHLLKLTQKPQIDASDALAIALCHAHTRSSLIPH
GLGTARSRGGRLRL

Sequences:

>Translated_174_residues
MTLILGIDPGSRITGYGVVRDTGRGCVYVASGCIRTGSGELHERLQIVYRGVREVIKTYEPVTMGIEKVFMARNADSALK
LGQARGAAIVAGAEEALEIAEYTATQVKQAVAGTGGANKEQVMMMVMHLLKLTQKPQIDASDALAIALCHAHTRSSLIPH
GLGTARSRGGRLRL
>Mature_173_residues
TLILGIDPGSRITGYGVVRDTGRGCVYVASGCIRTGSGELHERLQIVYRGVREVIKTYEPVTMGIEKVFMARNADSALKL
GQARGAAIVAGAEEALEIAEYTATQVKQAVAGTGGANKEQVMMMVMHLLKLTQKPQIDASDALAIALCHAHTRSSLIPHG
LGTARSRGGRLRL

Specific function: Nuclease that resolves Holliday junction intermediates in genetic recombination. Cleaves the cruciform structure in supercoiled DNA by nicking to strands with the same polarity at sites symmetrically opposed at the junction in the homologous arms and leav

COG id: COG0817

COG function: function code L; Holliday junction resolvasome, endonuclease subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ruvC family [H]

Homologues:

Organism=Escherichia coli, GI1788170, Length=173, Percent_Identity=54.3352601156069, Blast_Score=167, Evalue=5e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012337
- InterPro:   IPR020563
- InterPro:   IPR002176 [H]

Pfam domain/function: PF02075 RuvC [H]

EC number: =3.1.22.4 [H]

Molecular weight: Translated: 18516; Mature: 18385

Theoretical pI: Translated: 9.77; Mature: 9.77

Prosite motif: PS01321 RUVC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
5.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
5.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLILGIDPGSRITGYGVVRDTGRGCVYVASGCIRTGSGELHERLQIVYRGVREVIKTYE
CEEEEECCCCCCEEEEEEEEECCCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCC
PVTMGIEKVFMARNADSALKLGQARGAAIVAGAEEALEIAEYTATQVKQAVAGTGGANKE
CHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVMMMVMHLLKLTQKPQIDASDALAIALCHAHTRSSLIPHGLGTARSRGGRLRL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC
>Mature Secondary Structure 
TLILGIDPGSRITGYGVVRDTGRGCVYVASGCIRTGSGELHERLQIVYRGVREVIKTYE
EEEEECCCCCCEEEEEEEEECCCCEEEEECCCEECCCHHHHHHHHHHHHHHHHHHHHCC
PVTMGIEKVFMARNADSALKLGQARGAAIVAGAEEALEIAEYTATQVKQAVAGTGGANKE
CHHHHHHHHHHHCCCHHHHHHHCCCCCEEEECHHHHHHHHHHHHHHHHHHHHCCCCCCHH
QVMMMVMHLLKLTQKPQIDASDALAIALCHAHTRSSLIPHGLGTARSRGGRLRL
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]