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The map label for this gene is mltF

Identifier: 66044516

GI number: 66044516

Start: 1431627

End: 1433123

Strand: Reverse

Name: mltF

Synonym: Psyr_1268

Alternate gene names: 66044516

Gene position: 1433123-1431627 (Counterclockwise)

Preceding gene: 66044519

Following gene: 66044507

Centisome position: 23.52

GC content: 58.65

Gene sequence:

>1497_bases
ATGTTCTTCAAACCAGATTTCCGCCCACGTTGCGCCAAATGGCTCATCGCAACCGGACTCTTCCTGATGCTCGGCGCCTG
TGTGGAAAAACCCACCACACTGGAGCGAGTCAAGGAGGACGGCGTACTGCGGGTGATCACCCGCAACAGCCCGGCGACTT
ATTTCCAGGATCGCAACGGTGAAACCGGCTTCGAATACGAGCTGGTCAAGCGCTTTGCCGATGATCTGGGCGTCGAACTG
AAGATAGAAACAGCTGACAATCTTGACGATCTTTTCGACCAGATGAACAAACCGGGCGGTCCGGTACTCGGTGCCGCCGG
CCTGATTGAAACGCCACTGCGCAAGCAACAGGCGCGTTTCTCGCACTCTTATCTGGAAGTCACCCCTCAGGTGGTCTATC
GCAACGGCCAGTCGCGACCGACCGATCCCGGCGATCTGGTGGGCAAACGCATCGTGGTGCTCAAAGGCAGCGCGCATGCC
GAGCAACTGGCGGCGCTCAAGGCGCAGAATCCCGGCATTCAATATGAAGAATCGGACGCGGTCGAGGTCGTTGATCTCCT
GCGCATGGTCGACGAAGGCCAGATCGATCTGACCCTGGTCGACTCCAATGAGCTGGCAATGAACCAGGTCTACTTCCCCA
ATGTACGCGTAGCCTTTGACCTGGGCGAAGCCCGTGCACAGCGCTGGGCCGTCGCGCCGGGTGAAGACAACAGCCTGCTC
AACGAAATCAACGCCTACCTCGACAAGGTCGAAAAGAACGGCACCCTGCAACGCCTGAAGGACCGTTACTACGGCCACGT
CGACGTGCTCGGCTATGTCGGTGCCTACACCTTTGCCCAGCACCTTCAGGAACGCCTGCCCAAATACGAGAAGCACTTCC
AGACATCGGCCAAGAAAGAACAGGTCGACTGGAGACTGCTGGCCGCCATCGGCTATCAGGAATCGATGTGGCAGCCGACC
GTGACCTCGAAAACCGGCGTGCGCGGCCTGATGATGCTCACCCAGAACACCGCGCAAGCCATGGGCGTGTCCAACAGGCT
GGATGCACGCCAGAGCATTCAGGGCGGGGCAAAGTACTTTGCCTACATAAAAGATCAGCTGGACGATTCGATCAAAGAGC
CGGATCGCACATGGCTCGCTCTCGCTTCCTACAACATTGGCAGCGGTCACCTTGAAGATGCGCGCAAATTGGCCCAGAAC
GAGGGGCTGAATCCTGATAAATGGCTCGACGTAAAAAAAATGCTGCCGCGTCTGGCACAGAAGAAGTGGTACAGCAAAAC
GCGTTACGGCTACGCCCGAGGCGGTGAGCCGGTGCATTTCGTGGCCAACATTCGCCGTTATTACGACATTCTGACCTGGG
TGACGCAGCCCCAGCTTGAAGGCAGTCAGGTCGCCGATGGCAACCTGCATGTTCCCGGCGTCGACAAGACCCAACCGCCC
GTCCCGCCAGCCTCCCCCGTCCCCTCGTCCAGCAGCACGGACGAATCTCCGCTCTAA

Upstream 100 bases:

>100_bases
CAAAAAAACTCCGGAAATGCCGGTAAAAGCCACTCGCCTATCAGACGCAAGCTTCACTGTCGAGATATGGCGCCCGGTGC
GCTTTGCGTATACTGCACCG

Downstream 100 bases:

>100_bases
ACGTTGCCCTGGCCTTTGTCAGCGTCCTTGGCTGCCCGGCGCATTCTGAAAAACTCGCTCAGCATCGTCCCGCACTCCTC
GCCCAGCACCCCGCCTTCGA

Product: putative transglycosylase

Products: NA

Alternate protein names: Murein lyase F

Number of amino acids: Translated: 498; Mature: 498

Protein sequence:

>498_residues
MFFKPDFRPRCAKWLIATGLFLMLGACVEKPTTLERVKEDGVLRVITRNSPATYFQDRNGETGFEYELVKRFADDLGVEL
KIETADNLDDLFDQMNKPGGPVLGAAGLIETPLRKQQARFSHSYLEVTPQVVYRNGQSRPTDPGDLVGKRIVVLKGSAHA
EQLAALKAQNPGIQYEESDAVEVVDLLRMVDEGQIDLTLVDSNELAMNQVYFPNVRVAFDLGEARAQRWAVAPGEDNSLL
NEINAYLDKVEKNGTLQRLKDRYYGHVDVLGYVGAYTFAQHLQERLPKYEKHFQTSAKKEQVDWRLLAAIGYQESMWQPT
VTSKTGVRGLMMLTQNTAQAMGVSNRLDARQSIQGGAKYFAYIKDQLDDSIKEPDRTWLALASYNIGSGHLEDARKLAQN
EGLNPDKWLDVKKMLPRLAQKKWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGSQVADGNLHVPGVDKTQPP
VPPASPVPSSSSTDESPL

Sequences:

>Translated_498_residues
MFFKPDFRPRCAKWLIATGLFLMLGACVEKPTTLERVKEDGVLRVITRNSPATYFQDRNGETGFEYELVKRFADDLGVEL
KIETADNLDDLFDQMNKPGGPVLGAAGLIETPLRKQQARFSHSYLEVTPQVVYRNGQSRPTDPGDLVGKRIVVLKGSAHA
EQLAALKAQNPGIQYEESDAVEVVDLLRMVDEGQIDLTLVDSNELAMNQVYFPNVRVAFDLGEARAQRWAVAPGEDNSLL
NEINAYLDKVEKNGTLQRLKDRYYGHVDVLGYVGAYTFAQHLQERLPKYEKHFQTSAKKEQVDWRLLAAIGYQESMWQPT
VTSKTGVRGLMMLTQNTAQAMGVSNRLDARQSIQGGAKYFAYIKDQLDDSIKEPDRTWLALASYNIGSGHLEDARKLAQN
EGLNPDKWLDVKKMLPRLAQKKWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGSQVADGNLHVPGVDKTQPP
VPPASPVPSSSSTDESPL
>Mature_498_residues
MFFKPDFRPRCAKWLIATGLFLMLGACVEKPTTLERVKEDGVLRVITRNSPATYFQDRNGETGFEYELVKRFADDLGVEL
KIETADNLDDLFDQMNKPGGPVLGAAGLIETPLRKQQARFSHSYLEVTPQVVYRNGQSRPTDPGDLVGKRIVVLKGSAHA
EQLAALKAQNPGIQYEESDAVEVVDLLRMVDEGQIDLTLVDSNELAMNQVYFPNVRVAFDLGEARAQRWAVAPGEDNSLL
NEINAYLDKVEKNGTLQRLKDRYYGHVDVLGYVGAYTFAQHLQERLPKYEKHFQTSAKKEQVDWRLLAAIGYQESMWQPT
VTSKTGVRGLMMLTQNTAQAMGVSNRLDARQSIQGGAKYFAYIKDQLDDSIKEPDRTWLALASYNIGSGHLEDARKLAQN
EGLNPDKWLDVKKMLPRLAQKKWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGSQVADGNLHVPGVDKTQPP
VPPASPVPSSSSTDESPL

Specific function: Murein-degrading enzyme that degrades murein glycan strands and insoluble, high-molecular weight murein sacculi, with the concomitant formation of a 1,6-anhydromuramoyl product. Lytic transglycosylases (LTs) play an integral role in the metabolism of the

COG id: COG4623

COG function: function code M; Predicted soluble lytic transglycosylase fused to an ABC-type amino acid-binding protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein. Note=Attached to the inner leaflet of the outer membrane

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transglycosylase slt family

Homologues:

Organism=Escherichia coli, GI171474010, Length=412, Percent_Identity=39.8058252427184, Blast_Score=301, Evalue=7e-83,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): MLTF_PSEU2 (Q4ZX03)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_234357.1
- ProteinModelPortal:   Q4ZX03
- SMR:   Q4ZX03
- STRING:   Q4ZX03
- GeneID:   3366763
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_1268
- NMPDR:   fig|205918.4.peg.1582
- eggNOG:   COG4623
- HOGENOM:   HBG644469
- OMA:   QYVENIR
- ProtClustDB:   PRK10859
- BioCyc:   PSYR205918:PSYR_1268-MONOMER
- HAMAP:   MF_02016
- InterPro:   IPR008258
- InterPro:   IPR001638
- InterPro:   IPR000189
- SMART:   SM00062

Pfam domain/function: PF00497 SBP_bac_3; PF01464 SLT

EC number: NA

Molecular weight: Translated: 55898; Mature: 55898

Theoretical pI: Translated: 6.53; Mature: 6.53

Prosite motif: PS51257 PROKAR_LIPOPROTEIN; PS00922 TRANSGLYCOSYLASE; PS00013 PROKAR_LIPOPROTEIN

Important sites: ACT_SITE 314-314

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFFKPDFRPRCAKWLIATGLFLMLGACVEKPTTLERVKEDGVLRVITRNSPATYFQDRNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCEEECCCC
ETGFEYELVKRFADDLGVELKIETADNLDDLFDQMNKPGGPVLGAAGLIETPLRKQQARF
CCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHH
SHSYLEVTPQVVYRNGQSRPTDPGDLVGKRIVVLKGSAHAEQLAALKAQNPGIQYEESDA
HHHHHHHCHHHHEECCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCH
VEVVDLLRMVDEGQIDLTLVDSNELAMNQVYFPNVRVAFDLGEARAQRWAVAPGEDNSLL
HHHHHHHHHHCCCCEEEEEECCCCEEECEEECCCEEEEEECCHHHHHHCCCCCCCCHHHH
NEINAYLDKVEKNGTLQRLKDRYYGHVDVLGYVGAYTFAQHLQERLPKYEKHFQTSAKKE
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QVDWRLLAAIGYQESMWQPTVTSKTGVRGLMMLTQNTAQAMGVSNRLDARQSIQGGAKYF
HHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCHHHH
AYIKDQLDDSIKEPDRTWLALASYNIGSGHLEDARKLAQNEGLNPDKWLDVKKMLPRLAQ
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KKWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGSQVADGNLHVPGVDKTQPP
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCC
VPPASPVPSSSSTDESPL
CCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MFFKPDFRPRCAKWLIATGLFLMLGACVEKPTTLERVKEDGVLRVITRNSPATYFQDRNG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHCCCEEEEEECCCCCCEEECCCC
ETGFEYELVKRFADDLGVELKIETADNLDDLFDQMNKPGGPVLGAAGLIETPLRKQQARF
CCCHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHCCCCCCEEECCHHHHHHHHHHHHHH
SHSYLEVTPQVVYRNGQSRPTDPGDLVGKRIVVLKGSAHAEQLAALKAQNPGIQYEESDA
HHHHHHHCHHHHEECCCCCCCCCHHHCCCEEEEEECCCCHHHHHHHHCCCCCCCCCCCCH
VEVVDLLRMVDEGQIDLTLVDSNELAMNQVYFPNVRVAFDLGEARAQRWAVAPGEDNSLL
HHHHHHHHHHCCCCEEEEEECCCCEEECEEECCCEEEEEECCHHHHHHCCCCCCCCHHHH
NEINAYLDKVEKNGTLQRLKDRYYGHVDVLGYVGAYTFAQHLQERLPKYEKHFQTSAKKE
HHHHHHHHHHHCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHH
QVDWRLLAAIGYQESMWQPTVTSKTGVRGLMMLTQNTAQAMGVSNRLDARQSIQGGAKYF
HHHHHHHHHHCCHHHCCCCCCCCCCCCCEEEEEHHHHHHHHCCHHHHHHHHHHHCCHHHH
AYIKDQLDDSIKEPDRTWLALASYNIGSGHLEDARKLAQNEGLNPDKWLDVKKMLPRLAQ
HHHHHHHHHHHCCCCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
KKWYSKTRYGYARGGEPVHFVANIRRYYDILTWVTQPQLEGSQVADGNLHVPGVDKTQPP
HHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEECCCCCCCCCC
VPPASPVPSSSSTDESPL
CCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA