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The map label for this gene is ispG [H]

Identifier: 66044496

GI number: 66044496

Start: 1403345

End: 1404454

Strand: Direct

Name: ispG [H]

Synonym: Psyr_1248

Alternate gene names: 66044496

Gene position: 1403345-1404454 (Clockwise)

Preceding gene: 66044495

Following gene: 66044497

Centisome position: 23.03

GC content: 60.0

Gene sequence:

>1110_bases
ATGCACGGCGAATCTCCAATCAAGCGTCGCGAATCCCGGAAAATATGGGTCGGTTCCGTACCGGTAGGCGGTGATGCGCC
GATCGCGGTGCAGAGCATGACCAACAGCGATACCAACGATGTCGCCGCTACCGTGGCACAGATCAATCGTCTGGAAGCGG
CGGGTGTCGACATCGTGCGTGTTTCGGTGCCGGACATGGACGCTGCCGAAGCATTCGGCCGCATCAAGCAACTGGTCAAG
GTGCCGCTGGTCGCCGACATTCACTTCGATCACCGCATCGCCTTGCGTGTGGCGGAGCTGGGCGTCGACTGTCTGCGTAT
CAACCCGGGCAACATCGGTCGCGAAGACCGTGTGCGGGCGGTGGTCGATGCTGCACGTGATCGGGGCATCCCGATCCGTA
TCGGCGTCAATGCCGGTTCGCTGGAAAAAGACCTGCAGAAGAAGTACGGCGAGCCCACGCCAGAGGCCCTGGTAGAGTCT
GCGCTGCGCCATGTCGAGCATCTGCAACGCCTGAACTTTCAGGACTTCAAGGTCAGCGTCAAGGCTTCCGATGTGTTCAT
GGCGGTCGCGGCCTATCGGCTGCTGGCCAAGGAGATTGTTCAGCCGCTGCACCTGGGCATCACCGAAGCCGGTGGTCTGC
GTTCGGGTACGGTGAAATCGGCTGTCGGTCTCGGTATGCTGCTTGCCGAAGGAATTGGCGATACTATCCGCATCTCGCTG
GCGGCTGACCCGGTCGAGGAAGTGAAGGTCGGGTACGACATTCTCAAATCGTTGCGCCTTCGTTCGCGTGGAATCAACTT
CATCGCCTGTCCGAGCTGCTCGCGGCAGAACTTCGATGTGGTCAAGACCATGAACGAGCTGGAAGGTCGACTCGAGGATT
TGCTGGTGCCGCTCGATGTCGCGGTGATCGGTTGTGTGGTCAATGGTCCGGGCGAAGCCAAAGAGGCGCACATCGGCCTG
ACCGGTGGCACGCCGAACCTGATCTACATCGACGGCAAACCCGCGCAGAAACTGACCAATGACAATCTCGTGAATGAGCT
GGAACGCTTGATTCGCGAGAAAGCGGCTGAAAAAGCCGAAGCCGACGCCTCGGTCATCGTGCGCGGCTAA

Upstream 100 bases:

>100_bases
CTACGCGCGCGGTGCGCAGGTCAGCTACAACGGTCAGCCTGTGGATGTAGCACCTTTTACCAGCGGCGAAACTGCTCGCC
TGAAGCTAGGGCAATAAGTC

Downstream 100 bases:

>100_bases
CCCGGATTGCTAAGGATTTTTTGTGAGTAAGTCTCTACAAGCCATTCGTGGCATGAACGACATTCTGCCCGAGCAGACGC
CGCTGTGGCGTCACTTCGAA

Product: 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase

Products: NA

Alternate protein names: 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate synthase [H]

Number of amino acids: Translated: 369; Mature: 369

Protein sequence:

>369_residues
MHGESPIKRRESRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAATVAQINRLEAAGVDIVRVSVPDMDAAEAFGRIKQLVK
VPLVADIHFDHRIALRVAELGVDCLRINPGNIGREDRVRAVVDAARDRGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
ALRHVEHLQRLNFQDFKVSVKASDVFMAVAAYRLLAKEIVQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISL
AADPVEEVKVGYDILKSLRLRSRGINFIACPSCSRQNFDVVKTMNELEGRLEDLLVPLDVAVIGCVVNGPGEAKEAHIGL
TGGTPNLIYIDGKPAQKLTNDNLVNELERLIREKAAEKAEADASVIVRG

Sequences:

>Translated_369_residues
MHGESPIKRRESRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAATVAQINRLEAAGVDIVRVSVPDMDAAEAFGRIKQLVK
VPLVADIHFDHRIALRVAELGVDCLRINPGNIGREDRVRAVVDAARDRGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
ALRHVEHLQRLNFQDFKVSVKASDVFMAVAAYRLLAKEIVQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISL
AADPVEEVKVGYDILKSLRLRSRGINFIACPSCSRQNFDVVKTMNELEGRLEDLLVPLDVAVIGCVVNGPGEAKEAHIGL
TGGTPNLIYIDGKPAQKLTNDNLVNELERLIREKAAEKAEADASVIVRG
>Mature_369_residues
MHGESPIKRRESRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAATVAQINRLEAAGVDIVRVSVPDMDAAEAFGRIKQLVK
VPLVADIHFDHRIALRVAELGVDCLRINPGNIGREDRVRAVVDAARDRGIPIRIGVNAGSLEKDLQKKYGEPTPEALVES
ALRHVEHLQRLNFQDFKVSVKASDVFMAVAAYRLLAKEIVQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISL
AADPVEEVKVGYDILKSLRLRSRGINFIACPSCSRQNFDVVKTMNELEGRLEDLLVPLDVAVIGCVVNGPGEAKEAHIGL
TGGTPNLIYIDGKPAQKLTNDNLVNELERLIREKAAEKAEADASVIVRG

Specific function: Converts 2C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-2,4cPP) into 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate [H]

COG id: COG0821

COG function: function code I; Enzyme involved in the deoxyxylulose pathway of isoprenoid biosynthesis

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispG family [H]

Homologues:

Organism=Escherichia coli, GI1788863, Length=362, Percent_Identity=70.9944751381215, Blast_Score=530, Evalue=1e-152,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR011005
- InterPro:   IPR016425
- InterPro:   IPR004588 [H]

Pfam domain/function: PF04551 GcpE [H]

EC number: =1.17.7.1 [H]

Molecular weight: Translated: 39751; Mature: 39751

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGESPIKRRESRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAATVAQINRLEAAGVDIVR
CCCCCCCHHCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEE
VSVPDMDAAEAFGRIKQLVKVPLVADIHFDHRIALRVAELGVDCLRINPGNIGREDRVRA
EECCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
VVDAARDRGIPIRIGVNAGSLEKDLQKKYGEPTPEALVESALRHVEHLQRLNFQDFKVSV
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFMAVAAYRLLAKEIVQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISL
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEVKVGYDILKSLRLRSRGINFIACPSCSRQNFDVVKTMNELEGRLEDLLVPLDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVIGCVVNGPGEAKEAHIGLTGGTPNLIYIDGKPAQKLTNDNLVNELERLIREKAAEKAE
EEEEEEECCCCCCCEEEEEEECCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHC
ADASVIVRG
CCCEEEEEC
>Mature Secondary Structure
MHGESPIKRRESRKIWVGSVPVGGDAPIAVQSMTNSDTNDVAATVAQINRLEAAGVDIVR
CCCCCCCHHCCCCEEEEECCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHHHHCCCEEEE
VSVPDMDAAEAFGRIKQLVKVPLVADIHFDHRIALRVAELGVDCLRINPGNIGREDRVRA
EECCCCHHHHHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCEEEEECCCCCCHHHHHHH
VVDAARDRGIPIRIGVNAGSLEKDLQKKYGEPTPEALVESALRHVEHLQRLNFQDFKVSV
HHHHHHCCCCEEEEECCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEE
KASDVFMAVAAYRLLAKEIVQPLHLGITEAGGLRSGTVKSAVGLGMLLAEGIGDTIRISL
EHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEE
AADPVEEVKVGYDILKSLRLRSRGINFIACPSCSRQNFDVVKTMNELEGRLEDLLVPLDV
ECCCHHHHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
AVIGCVVNGPGEAKEAHIGLTGGTPNLIYIDGKPAQKLTNDNLVNELERLIREKAAEKAE
EEEEEEECCCCCCCEEEEEEECCCCCEEEECCCCHHHHCCCHHHHHHHHHHHHHHHHHHC
ADASVIVRG
CCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA