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The map label for this gene is iscS

Identifier: 66044485

GI number: 66044485

Start: 1394772

End: 1395986

Strand: Direct

Name: iscS

Synonym: Psyr_1237

Alternate gene names: 66044485

Gene position: 1394772-1395986 (Clockwise)

Preceding gene: 66044484

Following gene: 66044486

Centisome position: 22.89

GC content: 58.93

Gene sequence:

>1215_bases
ATGAAATTGCCAATTTACCTCGACTACTCCGCGACGACCCCGGTCGATCCGCGCGTCGCGCAGAAAATGATCGATTGCCT
GACCGTCGAAGGAAACTTCGGCAACCCGGCCTCGCGCTCCCACGTCTTTGGCTGGAAAGCCGAAGAAGCGGTCGAGAACG
CCCGTCGTCAGGTCGCTGATCTGGTCAACGCCGATCCGCGCGAAATCGTCTGGACCTCGGGTGCTACCGAATCCAACAAC
CTGGCTATCAAGGGTGTTGCGCATTTCTATGCCAGCAAAGGCAAGCACCTGATCACCTCCAAGGTTGAACACAAGGCTGT
CCTCGACACCACTCGCCAGCTGGAACGCGAAGGTTTCGAAGTGACCTACATCGAGCCCGGTGAAGACGGTCTGATCACGC
CTGCCATGATTGAAGCGGCACTGCGCGACGACACCATTCTGGTTTCGATCATGCATGTGAACAACGAGATCGGCACGATC
AACGATATTGCCGCGATCGGTGAACTGACCCGTTCGCGCGGTGTGCTGTTCCACGTCGATGGCGCGCAGTCGACCGGCAA
GGTTGAAATCGATCTGGCCAGCCTCAAGGTTGACCTGATGTCGTTCTCTGCACACAAGACCTACGGCCCTAAAGGCATCG
GCGCACTCTACGTCAGCCGCAAGCCACGTGTTCGCCTTGAGGCGACCATGCACGGCGGCGGTCACGAGCGCGGCATGCGT
TCCGGCACCCTGGCGACCCACCAGATCGTCGGCATGGGCGAAGCATTCCGTATCGCCAAGGAAGAAATGGCTGCTGAAAA
CGTGCGCATCAAGGCACTCAGCGATCGTTTCTTCAAGCAGGTCGAAAATCTGGAAGAGCTGTACGTCAACGGCAGCCTGA
CTGCCCGCGTACCGCACAACCTGAACCTGAGCTTCAACTATGTCGAAGGCGAGTCGCTGATCATGGCACTCAAGGATCTG
GCGGTATCGTCCGGTTCGGCCTGTACTTCGGCCTCGCTGGAGCCCTCGTACGTGCTGCGTGCCCTGGGTCGCAACGACGA
ACTGGCGCACAGCTCGATTCGTTTCACCTTCGGCCGGTTCAGCACCGAAGAAGAAATCGATTACGCCGCGCAGAAGGTTT
GCGAGGCTGTCACCCGGCTGCGCACCCTTTCGCCGCTGTGGGACATGTTCAAAGACGGCGTCGACATCTCCAAGATCGAG
TGGGCGGCGCACTAA

Upstream 100 bases:

>100_bases
GTGGTCATAATCACATGTCGCAACTGGGTAAGATCGAAACGTCCGCCGTCGAATGAACGCAGATGAATGAGCGGTGCGCC
TGCCTGATAGGAGAGCTTCA

Downstream 100 bases:

>100_bases
TTTAAAGTCGCCTCCAATACAGGTCATGGGCAATTGCGCGTGACCTGCAGAGCGGCTCCCTGATGTGAGAGGAATAGAAT
CATGGCTTACAGCGAAAAGG

Product: cysteine desulfurase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 404

Protein sequence:

>404_residues
MKLPIYLDYSATTPVDPRVAQKMIDCLTVEGNFGNPASRSHVFGWKAEEAVENARRQVADLVNADPREIVWTSGATESNN
LAIKGVAHFYASKGKHLITSKVEHKAVLDTTRQLEREGFEVTYIEPGEDGLITPAMIEAALRDDTILVSIMHVNNEIGTI
NDIAAIGELTRSRGVLFHVDGAQSTGKVEIDLASLKVDLMSFSAHKTYGPKGIGALYVSRKPRVRLEATMHGGGHERGMR
SGTLATHQIVGMGEAFRIAKEEMAAENVRIKALSDRFFKQVENLEELYVNGSLTARVPHNLNLSFNYVEGESLIMALKDL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFSTEEEIDYAAQKVCEAVTRLRTLSPLWDMFKDGVDISKIE
WAAH

Sequences:

>Translated_404_residues
MKLPIYLDYSATTPVDPRVAQKMIDCLTVEGNFGNPASRSHVFGWKAEEAVENARRQVADLVNADPREIVWTSGATESNN
LAIKGVAHFYASKGKHLITSKVEHKAVLDTTRQLEREGFEVTYIEPGEDGLITPAMIEAALRDDTILVSIMHVNNEIGTI
NDIAAIGELTRSRGVLFHVDGAQSTGKVEIDLASLKVDLMSFSAHKTYGPKGIGALYVSRKPRVRLEATMHGGGHERGMR
SGTLATHQIVGMGEAFRIAKEEMAAENVRIKALSDRFFKQVENLEELYVNGSLTARVPHNLNLSFNYVEGESLIMALKDL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFSTEEEIDYAAQKVCEAVTRLRTLSPLWDMFKDGVDISKIE
WAAH
>Mature_404_residues
MKLPIYLDYSATTPVDPRVAQKMIDCLTVEGNFGNPASRSHVFGWKAEEAVENARRQVADLVNADPREIVWTSGATESNN
LAIKGVAHFYASKGKHLITSKVEHKAVLDTTRQLEREGFEVTYIEPGEDGLITPAMIEAALRDDTILVSIMHVNNEIGTI
NDIAAIGELTRSRGVLFHVDGAQSTGKVEIDLASLKVDLMSFSAHKTYGPKGIGALYVSRKPRVRLEATMHGGGHERGMR
SGTLATHQIVGMGEAFRIAKEEMAAENVRIKALSDRFFKQVENLEELYVNGSLTARVPHNLNLSFNYVEGESLIMALKDL
AVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRFSTEEEIDYAAQKVCEAVTRLRTLSPLWDMFKDGVDISKIE
WAAH

Specific function: Catalyzes the removal of elemental sulfur from cysteine to produce alanine

COG id: COG1104

COG function: function code E; Cysteine sulfinate desulfinase/cysteine desulfurase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. NifS/IscS subfamily

Homologues:

Organism=Homo sapiens, GI32307132, Length=402, Percent_Identity=56.4676616915423, Blast_Score=485, Evalue=1e-137,
Organism=Homo sapiens, GI156713448, Length=414, Percent_Identity=32.6086956521739, Blast_Score=183, Evalue=3e-46,
Organism=Escherichia coli, GI48994898, Length=404, Percent_Identity=73.5148514851485, Blast_Score=630, Evalue=0.0,
Organism=Escherichia coli, GI1787970, Length=398, Percent_Identity=27.3869346733668, Blast_Score=98, Evalue=7e-22,
Organism=Escherichia coli, GI1789175, Length=168, Percent_Identity=32.7380952380952, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI25143064, Length=403, Percent_Identity=55.8312655086849, Blast_Score=462, Evalue=1e-130,
Organism=Caenorhabditis elegans, GI17533177, Length=329, Percent_Identity=31.9148936170213, Blast_Score=145, Evalue=4e-35,
Organism=Saccharomyces cerevisiae, GI6319831, Length=401, Percent_Identity=55.3615960099751, Blast_Score=467, Evalue=1e-132,
Organism=Drosophila melanogaster, GI20129463, Length=403, Percent_Identity=55.5831265508685, Blast_Score=457, Evalue=1e-129,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): ISCS_PSE14 (Q48M05)

Other databases:

- EMBL:   CP000058
- RefSeq:   YP_273567.1
- ProteinModelPortal:   Q48M05
- SMR:   Q48M05
- STRING:   Q48M05
- GeneID:   3559956
- GenomeReviews:   CP000058_GR
- KEGG:   psp:PSPPH_1309
- eggNOG:   COG1104
- HOGENOM:   HBG635316
- OMA:   DMSPLWD
- PhylomeDB:   Q48M05
- ProtClustDB:   PRK14012
- HAMAP:   MF_00331
- InterPro:   IPR000192
- InterPro:   IPR020578
- InterPro:   IPR010240
- InterPro:   IPR016454
- InterPro:   IPR015424
- InterPro:   IPR015421
- InterPro:   IPR015422
- Gene3D:   G3DSA:3.40.640.10
- Gene3D:   G3DSA:3.90.1150.10
- PIRSF:   PIRSF005572
- TIGRFAMs:   TIGR02006

Pfam domain/function: PF00266 Aminotran_5; SSF53383 PyrdxlP-dep_Trfase_major

EC number: =2.8.1.7

Molecular weight: Translated: 44453; Mature: 44453

Theoretical pI: Translated: 6.20; Mature: 6.20

Prosite motif: PS00595 AA_TRANSFER_CLASS_5

Important sites: ACT_SITE 328-328

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLPIYLDYSATTPVDPRVAQKMIDCLTVEGNFGNPASRSHVFGWKAEEAVENARRQVAD
CCCEEEEECCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LVNADPREIVWTSGATESNNLAIKGVAHFYASKGKHLITSKVEHKAVLDTTRQLEREGFE
HHCCCCCCEEEECCCCCCCCEEEEEHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCE
VTYIEPGEDGLITPAMIEAALRDDTILVSIMHVNNEIGTINDIAAIGELTRSRGVLFHVD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEC
GAQSTGKVEIDLASLKVDLMSFSAHKTYGPKGIGALYVSRKPRVRLEATMHGGGHERGMR
CCCCCCEEEEEEEHEEEEEHHCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCC
SGTLATHQIVGMGEAFRIAKEEMAAENVRIKALSDRFFKQVENLEELYVNGSLTARVPHN
CCCCHHHHEECCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHEEECCEEEEECCCC
LNLSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCC
STEEEIDYAAQKVCEAVTRLRTLSPLWDMFKDGVDISKIEWAAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC
>Mature Secondary Structure
MKLPIYLDYSATTPVDPRVAQKMIDCLTVEGNFGNPASRSHVFGWKAEEAVENARRQVAD
CCCEEEEECCCCCCCCHHHHHHHHHEEEEECCCCCCCCCCCCCCCCHHHHHHHHHHHHHH
LVNADPREIVWTSGATESNNLAIKGVAHFYASKGKHLITSKVEHKAVLDTTRQLEREGFE
HHCCCCCCEEEECCCCCCCCEEEEEHHHHHHCCCCEEEHHHHHHHHHHHHHHHHHHCCCE
VTYIEPGEDGLITPAMIEAALRDDTILVSIMHVNNEIGTINDIAAIGELTRSRGVLFHVD
EEEECCCCCCCCCHHHHHHHHCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHCCEEEEEC
GAQSTGKVEIDLASLKVDLMSFSAHKTYGPKGIGALYVSRKPRVRLEATMHGGGHERGMR
CCCCCCEEEEEEEHEEEEEHHCCCCCCCCCCCCEEEEECCCCCEEEEEEECCCCCCCCCC
SGTLATHQIVGMGEAFRIAKEEMAAENVRIKALSDRFFKQVENLEELYVNGSLTARVPHN
CCCCHHHHEECCCHHHHHHHHHHHHCCEEEEHHHHHHHHHHHHHHHEEECCEEEEECCCC
LNLSFNYVEGESLIMALKDLAVSSGSACTSASLEPSYVLRALGRNDELAHSSIRFTFGRF
CCEEEEEECCHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCEEEEEECCC
STEEEIDYAAQKVCEAVTRLRTLSPLWDMFKDGVDISKIEWAAH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA