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The map label for this gene is trmJ [H]

Identifier: 66044482

GI number: 66044482

Start: 1392536

End: 1393300

Strand: Direct

Name: trmJ [H]

Synonym: Psyr_1234

Alternate gene names: 66044482

Gene position: 1392536-1393300 (Clockwise)

Preceding gene: 66044480

Following gene: 66044483

Centisome position: 22.85

GC content: 63.27

Gene sequence:

>765_bases
TTGTTGCAGAACATTCGTGTTGTCCTGGTCGGCACTACCCATCCCGGAAATATCGGCGGGGCGGCTCGCGCCATGAAAAA
CATGGGGCTTTCGCGTCTGGTGCTGGTCGATCCGCGGATCTTCCCTTCGCCCGATGCCGATGCGCGTGCGTCCGGCGCTA
CCGACATCCTCGAGGGCGCGCAGGTGGTGGCGACCCTCGAAGAAGCGCTGGTCGGCTGCCGTCTGGTGCTGGGCACCAGC
GCGCGGGATCGAAGCCTGCCTTGGCCCATGCTGGATCCGCGCGCCTCGGGTGAAAAAGTCATCGAGCAGGCCGGTGAGGG
CGCCGAGGTGGCACTGGTGTTCGGTCGTGAGCACGCGGGCTTGACCAACGAAGAGCTGCAGCGCTGCCACTTCCATGTGC
ACATCCCGTCTGACCCTGCGTTCAGTTCGCTCAATCTGGCGGCTGCCGTACAGGTGCTCAGTTATGAGGTGCGCGTAGCC
TGGCTGGCTGCGGCGGAGCAAGGCGAGGCTTCAAGACCCGCGTCGGCGCACAATGCCGAGCTGGCGACGATGGACGAAAT
GGAAGGTTTCTACACCCATCTTGAGGCCACGCTTGTGGCCATCGGCTTTCTTGATCCGGAAAAGCCGCGGCATCTGATGG
CCCGGCTGCGCAGACTGTACGGGCGTAGCGAAGTCGAACGTTCCGAGCTGAGTATCCTGCGCGGTGTGCTCACCGAAACC
CAGAAAGCCGCACGCGGTGAACCCTACAAGCGAAAGGATCAGTGA

Upstream 100 bases:

>100_bases
CGCGGTCGCTTCTGCGGTTGCGGTCCGCACAGCTTCATAGCGTTAGCCGAGCCGTTTCTGTAAGATTTGCCCCCCTAATT
CGTGTCAGTGAGCGTTCGCT

Downstream 100 bases:

>100_bases
TGTTCGAGCGTCTGCGAGAAGACATTCAAAGCGTGTTCCACCGCGATCCCGCAGCGCGCAACGCCTTTGAAGTCCTGACC
TGCTATCCGGGGATGCACGC

Product: RNA methyltransferase TrmH, group 1

Products: NA

Alternate protein names: tRNA Cm32/Um32 methyltransferase [H]

Number of amino acids: Translated: 254; Mature: 254

Protein sequence:

>254_residues
MLQNIRVVLVGTTHPGNIGGAARAMKNMGLSRLVLVDPRIFPSPDADARASGATDILEGAQVVATLEEALVGCRLVLGTS
ARDRSLPWPMLDPRASGEKVIEQAGEGAEVALVFGREHAGLTNEELQRCHFHVHIPSDPAFSSLNLAAAVQVLSYEVRVA
WLAAAEQGEASRPASAHNAELATMDEMEGFYTHLEATLVAIGFLDPEKPRHLMARLRRLYGRSEVERSELSILRGVLTET
QKAARGEPYKRKDQ

Sequences:

>Translated_254_residues
MLQNIRVVLVGTTHPGNIGGAARAMKNMGLSRLVLVDPRIFPSPDADARASGATDILEGAQVVATLEEALVGCRLVLGTS
ARDRSLPWPMLDPRASGEKVIEQAGEGAEVALVFGREHAGLTNEELQRCHFHVHIPSDPAFSSLNLAAAVQVLSYEVRVA
WLAAAEQGEASRPASAHNAELATMDEMEGFYTHLEATLVAIGFLDPEKPRHLMARLRRLYGRSEVERSELSILRGVLTET
QKAARGEPYKRKDQ
>Mature_254_residues
MLQNIRVVLVGTTHPGNIGGAARAMKNMGLSRLVLVDPRIFPSPDADARASGATDILEGAQVVATLEEALVGCRLVLGTS
ARDRSLPWPMLDPRASGEKVIEQAGEGAEVALVFGREHAGLTNEELQRCHFHVHIPSDPAFSSLNLAAAVQVLSYEVRVA
WLAAAEQGEASRPASAHNAELATMDEMEGFYTHLEATLVAIGFLDPEKPRHLMARLRRLYGRSEVERSELSILRGVLTET
QKAARGEPYKRKDQ

Specific function: Catalyzes the formation of 2'O-methylated cytidine (Cm32) or 2'O-methylated uridine (Um32) at position 32 in tRNA [H]

COG id: COG0565

COG function: function code J; rRNA methylase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family [H]

Homologues:

Organism=Escherichia coli, GI1788881, Length=246, Percent_Identity=54.0650406504065, Blast_Score=267, Evalue=6e-73,
Organism=Escherichia coli, GI1790865, Length=182, Percent_Identity=35.7142857142857, Blast_Score=91, Evalue=7e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004384
- InterPro:   IPR001537 [H]

Pfam domain/function: PF00588 SpoU_methylase [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27592; Mature: 27592

Theoretical pI: Translated: 6.32; Mature: 6.32

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQNIRVVLVGTTHPGNIGGAARAMKNMGLSRLVLVDPRIFPSPDADARASGATDILEGA
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
QVVATLEEALVGCRLVLGTSARDRSLPWPMLDPRASGEKVIEQAGEGAEVALVFGREHAG
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCC
LTNEELQRCHFHVHIPSDPAFSSLNLAAAVQVLSYEVRVAWLAAAEQGEASRPASAHNAE
CCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCC
LATMDEMEGFYTHLEATLVAIGFLDPEKPRHLMARLRRLYGRSEVERSELSILRGVLTET
HHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QKAARGEPYKRKDQ
HHHHCCCCCCCCCC
>Mature Secondary Structure
MLQNIRVVLVGTTHPGNIGGAARAMKNMGLSRLVLVDPRIFPSPDADARASGATDILEGA
CCCCEEEEEEECCCCCCCCHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHH
QVVATLEEALVGCRLVLGTSARDRSLPWPMLDPRASGEKVIEQAGEGAEVALVFGREHAG
HHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCEEEEEEECCCCC
LTNEELQRCHFHVHIPSDPAFSSLNLAAAVQVLSYEVRVAWLAAAEQGEASRPASAHNAE
CCHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCCCCC
LATMDEMEGFYTHLEATLVAIGFLDPEKPRHLMARLRRLYGRSEVERSELSILRGVLTET
HHHHHHHHHHHHHHHHHHHEEECCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
QKAARGEPYKRKDQ
HHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA