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The map label for this gene is tgt [H]

Identifier: 66044476

GI number: 66044476

Start: 1386415

End: 1387548

Strand: Direct

Name: tgt [H]

Synonym: Psyr_1228

Alternate gene names: 66044476

Gene position: 1386415-1387548 (Clockwise)

Preceding gene: 66044475

Following gene: 66044477

Centisome position: 22.75

GC content: 60.05

Gene sequence:

>1134_bases
ATGAGTCGCACCTGTCGTATGTCTTTCGAGTTGTTGGCCACCGATGGCAAGGCCCGTCGCGGTCGGCTGACCTTCCCGCG
TGGTGTGGTGGAAACCCCGGCGTTCATGCCGGTGGGCACCTATGGCACGGTCAAGGGCATGCTGCCCCGTGATATCGAGG
CGACCGGCGCGCAGATGATTCTGGGCAATACGTTTCACCTGTGGCTGCGTCCGGGTACCGAAGTCATCAAGGGCCACGGC
GACCTGCACGATTTCATGCAGTGGAAGGGGCCGATTCTGACCGACTCCGGCGGTTTTCAGGTGTTCAGCCTGGGCGCCAT
GCGCAAGATCAAGGAAGAGGGCGTGACGTTTGCCTCGCCGGTCGATGGTGCCAAGGTGTTCATGGGGCCGGAAGAGTCGA
TGCAGGTCCAGCGTGATCTGGGTTCGGACGTGGTGATGATCTTCGACGAATGCACACCGTACCCGGCCGACGAAGATGTG
GCGCGTGTCTCCATGGAGCTGTCGCTGCGCTGGGCCAAGCGTTCCAAAATCGCCCACGGCGAAAACACTGCCGCGCTGTT
CGGCATCGTTCAGGGGGGCATGCACGAGAACCTGCGCAAGCGCTCGCTGGAAGGCCTGGACGAAATCGGTTTCGACGGTC
TGGCGATTGGCGGTCTGTCGGTAGGCGAACCCAAGCACGAGATGATCAAGGTGCTCGATTACCTGCCGGGCCTCATGCCG
GCAGACAAACCTCGTTACCTGATGGGTGTAGGCAAGCCGGAAGATCTGGTCGAAGGTGTGCGCCGTGGCGTCGACATGTT
CGACTGCGTGATGCCGACCCGCAACGCTCGCAACGGCCATCTGTTCACCGATACCGGTGTATTGAAAATCCGTAACGCGT
TCCATCGTCACGACAATTCGCCGCTGGATCCGACCTGCGATTGCTACACCTGCCAGAACTTCTCGCGCGCTTATCTGCAT
CACCTGGACAAGTGCGGGGAAATGCTGGGGAGCATGCTCAATACAATCCACAATTTGCGGCACTACCAGCTGCTTATGGC
TGGTTTGCGCGAGGCTATTCAACAGGGTACATTGGCCGCCTTCGTCGATGCCTTCTATGCCAAACGCGGCCTTCCAACGC
CGCCTCTGGGTTGA

Upstream 100 bases:

>100_bases
TCGAGCACGGCTACCGCTTCTTCAGTTACGGTGATGCGATGTTCATCACCCGCAATCCCGCGCCGACGGCCCCACAGGAA
TCGGCACCAGAGGATCACGC

Downstream 100 bases:

>100_bases
AACAGAACGAGTTTTTCCTGCTTTTTATACTAATTATGTAACTGGAGCGACAAATGAGCTTTTTCATCTCTCCCGCTTTT
GCGGATGCTGCTGCACCGGC

Product: queuine tRNA-ribosyltransferase

Products: NA

Alternate protein names: Guanine insertion enzyme; tRNA-guanine transglycosylase [H]

Number of amino acids: Translated: 377; Mature: 376

Protein sequence:

>377_residues
MSRTCRMSFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEATGAQMILGNTFHLWLRPGTEVIKGHG
DLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGAKVFMGPEESMQVQRDLGSDVVMIFDECTPYPADEDV
ARVSMELSLRWAKRSKIAHGENTAALFGIVQGGMHENLRKRSLEGLDEIGFDGLAIGGLSVGEPKHEMIKVLDYLPGLMP
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTDTGVLKIRNAFHRHDNSPLDPTCDCYTCQNFSRAYLH
HLDKCGEMLGSMLNTIHNLRHYQLLMAGLREAIQQGTLAAFVDAFYAKRGLPTPPLG

Sequences:

>Translated_377_residues
MSRTCRMSFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEATGAQMILGNTFHLWLRPGTEVIKGHG
DLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGAKVFMGPEESMQVQRDLGSDVVMIFDECTPYPADEDV
ARVSMELSLRWAKRSKIAHGENTAALFGIVQGGMHENLRKRSLEGLDEIGFDGLAIGGLSVGEPKHEMIKVLDYLPGLMP
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTDTGVLKIRNAFHRHDNSPLDPTCDCYTCQNFSRAYLH
HLDKCGEMLGSMLNTIHNLRHYQLLMAGLREAIQQGTLAAFVDAFYAKRGLPTPPLG
>Mature_376_residues
SRTCRMSFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEATGAQMILGNTFHLWLRPGTEVIKGHGD
LHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASPVDGAKVFMGPEESMQVQRDLGSDVVMIFDECTPYPADEDVA
RVSMELSLRWAKRSKIAHGENTAALFGIVQGGMHENLRKRSLEGLDEIGFDGLAIGGLSVGEPKHEMIKVLDYLPGLMPA
DKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTDTGVLKIRNAFHRHDNSPLDPTCDCYTCQNFSRAYLHH
LDKCGEMLGSMLNTIHNLRHYQLLMAGLREAIQQGTLAAFVDAFYAKRGLPTPPLG

Specific function: Exchanges the guanine residue with 7-aminomethyl-7- deazaguanine in tRNAs with GU(N) anticodons (tRNA-Asp, -Asn, -His and -Tyr). After this exchange, a cyclopentendiol moiety is attached to the 7-aminomethyl group of 7-deazaguanine, resulting in the hyper

COG id: COG0343

COG function: function code J; Queuine/archaeosine tRNA-ribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queuine tRNA-ribosyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI13654276, Length=359, Percent_Identity=43.1754874651811, Blast_Score=288, Evalue=7e-78,
Organism=Escherichia coli, GI1786607, Length=372, Percent_Identity=70.1612903225807, Blast_Score=558, Evalue=1e-160,
Organism=Caenorhabditis elegans, GI17544680, Length=360, Percent_Identity=41.1111111111111, Blast_Score=290, Evalue=8e-79,
Organism=Caenorhabditis elegans, GI25146502, Length=139, Percent_Identity=28.7769784172662, Blast_Score=74, Evalue=1e-13,
Organism=Drosophila melanogaster, GI19920510, Length=362, Percent_Identity=43.9226519337017, Blast_Score=306, Evalue=1e-83,
Organism=Drosophila melanogaster, GI21355311, Length=209, Percent_Identity=27.2727272727273, Blast_Score=88, Evalue=9e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004803
- InterPro:   IPR002616 [H]

Pfam domain/function: PF01702 TGT [H]

EC number: =2.4.2.29 [H]

Molecular weight: Translated: 41702; Mature: 41571

Theoretical pI: Translated: 7.16; Mature: 7.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
5.3 %Met     (Translated Protein)
7.2 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
5.1 %Met     (Mature Protein)
6.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRTCRMSFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEATGAQMI
CCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEE
LGNTFHLWLRPGTEVIKGHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASP
ECCEEEEEECCCHHHHHCCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECC
VDGAKVFMGPEESMQVQRDLGSDVVMIFDECTPYPADEDVARVSMELSLRWAKRSKIAHG
CCCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
ENTAALFGIVQGGMHENLRKRSLEGLDEIGFDGLAIGGLSVGEPKHEMIKVLDYLPGLMP
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTDTGVLKIRNAFHRHDNS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHCCCC
PLDPTCDCYTCQNFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQLLMAGLREAIQQGTLAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FVDAFYAKRGLPTPPLG
HHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SRTCRMSFELLATDGKARRGRLTFPRGVVETPAFMPVGTYGTVKGMLPRDIEATGAQMI
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCEEE
LGNTFHLWLRPGTEVIKGHGDLHDFMQWKGPILTDSGGFQVFSLGAMRKIKEEGVTFASP
ECCEEEEEECCCHHHHHCCCCHHHHHHCCCCEEECCCCCEEEEHHHHHHHHHCCCEEECC
VDGAKVFMGPEESMQVQRDLGSDVVMIFDECTPYPADEDVARVSMELSLRWAKRSKIAHG
CCCCEEEECCHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
ENTAALFGIVQGGMHENLRKRSLEGLDEIGFDGLAIGGLSVGEPKHEMIKVLDYLPGLMP
CCHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCHHHHHHHHHHCCCCCC
ADKPRYLMGVGKPEDLVEGVRRGVDMFDCVMPTRNARNGHLFTDTGVLKIRNAFHRHDNS
CCCCCEEEECCCHHHHHHHHHHCCHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHHCCCC
PLDPTCDCYTCQNFSRAYLHHLDKCGEMLGSMLNTIHNLRHYQLLMAGLREAIQQGTLAA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHH
FVDAFYAKRGLPTPPLG
HHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]