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The map label for this gene is queA [H]

Identifier: 66044475

GI number: 66044475

Start: 1385354

End: 1386418

Strand: Direct

Name: queA [H]

Synonym: Psyr_1227

Alternate gene names: 66044475

Gene position: 1385354-1386418 (Clockwise)

Preceding gene: 66044471

Following gene: 66044476

Centisome position: 22.73

GC content: 63.1

Gene sequence:

>1065_bases
ATGCGCGTCGCTGACTTTACCTTTGAACTCCCCGATTCCCTGATTGCTCGCCATCCACTGGCCGAGCGTCGCAGCAGTCG
TCTGTTGACCCTCGATGGGCCGACGGGCGCGCTGGCACATCGTCAATTCACCGATTTGCTCGAGCATCTGCGCCCGGGCG
ACTTGATGGTGTTCAACAATACCCGGGTCATTCCCGCACGCCTGTTCGGGCAAAAGGCCTCCGGCGGCAAGCTGGAGATT
CTGGTCGAGCGCGTGCTGGACAGCCATCGTGTGCTGGCGCATGTGCGCTCGAGCAAGTCGCCCAAGCCTGGCTCGTCGAT
TCTGATCGATGGCGGTGGCGAGGCCGAGATGGTGGCGCGGCACGATGCGTTGTTCGAGCTGCGCTTTGCCGAAGAAGTGC
TGCCGTTGCTCGAGCGTGTCGGCCGCATGCCGTTGCCTCCTTATATAGACCGTCCGGATGAAGGGGCGGATCGCGAGCGC
TATCAGACCGTTTACGCGCAGCGTGCGGGTGCGGTGGCTGCGCCGACTGCCGGCCTGCATTTCGATCAGCCGCTGCTCGA
GGCGATTGCTGCCAAGGGCGTTGAAACCGCGTTCGTTACTCTGCATGTCGGCGCAGGGACGTTCCAGCCGGTGCGCGTCG
AGCAGATCGAAGATCACCACATGCACAGCGAATGGCTGGAAGTTGGCCAGGACGTGGTCGACGCCGTCGCCGCGTGCCGT
GCACGGGGCGGGCGGGTGATTGCCGTCGGGACCACCAGCGTGCGCTCGCTGGAGAGTGCCGCGCGTGATGGCGAACTGAA
GTCGTTCAGTGGCGATACCGACATCTTCATCTATCCAGGCCGGCCGTTTCACGTGGTCGATGCCCTGGTGACCAATTTCC
ATTTGCCTGAATCCACGCTGTTGATGCTGGTTTCGGCGTTCGCCGGTTATCCCGAGACGATGGCGGCCTACGCGGCGGCC
ATCGAGCACGGCTACCGCTTCTTCAGTTACGGTGATGCGATGTTCATCACCCGCAATCCCGCGCCGACGGCCCCACAGGA
ATCGGCACCAGAGGATCACGCATGA

Upstream 100 bases:

>100_bases
TAAGGATGAGTTTTCTGTTGGTCAACAGGGATTTACGCAGGTTTTTTACTAATGCCGTCAAACCGGATAAACTTCCCGGC
CCTGCTAAACGAACCCGATC

Downstream 100 bases:

>100_bases
GTCGCACCTGTCGTATGTCTTTCGAGTTGTTGGCCACCGATGGCAAGGCCCGTCGCGGTCGGCTGACCTTCCCGCGTGGT
GTGGTGGAAACCCCGGCGTT

Product: S-adenosylmethionine:tRNA ribosyltransferase-isomerase

Products: NA

Alternate protein names: Queuosine biosynthesis protein queA [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MRVADFTFELPDSLIARHPLAERRSSRLLTLDGPTGALAHRQFTDLLEHLRPGDLMVFNNTRVIPARLFGQKASGGKLEI
LVERVLDSHRVLAHVRSSKSPKPGSSILIDGGGEAEMVARHDALFELRFAEEVLPLLERVGRMPLPPYIDRPDEGADRER
YQTVYAQRAGAVAAPTAGLHFDQPLLEAIAAKGVETAFVTLHVGAGTFQPVRVEQIEDHHMHSEWLEVGQDVVDAVAACR
ARGGRVIAVGTTSVRSLESAARDGELKSFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYAAA
IEHGYRFFSYGDAMFITRNPAPTAPQESAPEDHA

Sequences:

>Translated_354_residues
MRVADFTFELPDSLIARHPLAERRSSRLLTLDGPTGALAHRQFTDLLEHLRPGDLMVFNNTRVIPARLFGQKASGGKLEI
LVERVLDSHRVLAHVRSSKSPKPGSSILIDGGGEAEMVARHDALFELRFAEEVLPLLERVGRMPLPPYIDRPDEGADRER
YQTVYAQRAGAVAAPTAGLHFDQPLLEAIAAKGVETAFVTLHVGAGTFQPVRVEQIEDHHMHSEWLEVGQDVVDAVAACR
ARGGRVIAVGTTSVRSLESAARDGELKSFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYAAA
IEHGYRFFSYGDAMFITRNPAPTAPQESAPEDHA
>Mature_354_residues
MRVADFTFELPDSLIARHPLAERRSSRLLTLDGPTGALAHRQFTDLLEHLRPGDLMVFNNTRVIPARLFGQKASGGKLEI
LVERVLDSHRVLAHVRSSKSPKPGSSILIDGGGEAEMVARHDALFELRFAEEVLPLLERVGRMPLPPYIDRPDEGADRER
YQTVYAQRAGAVAAPTAGLHFDQPLLEAIAAKGVETAFVTLHVGAGTFQPVRVEQIEDHHMHSEWLEVGQDVVDAVAACR
ARGGRVIAVGTTSVRSLESAARDGELKSFSGDTDIFIYPGRPFHVVDALVTNFHLPESTLLMLVSAFAGYPETMAAYAAA
IEHGYRFFSYGDAMFITRNPAPTAPQESAPEDHA

Specific function: Transfers and isomerizes the ribose moiety from AdoMet to the 7-aminomethyl group of 7-deazaguanine (preQ1-tRNA) to give epoxyqueuosine (oQ-tRNA) [H]

COG id: COG0809

COG function: function code J; S-adenosylmethionine:tRNA-ribosyltransferase-isomerase (queuine synthetase)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the queA family [H]

Homologues:

Organism=Escherichia coli, GI1786606, Length=347, Percent_Identity=71.4697406340058, Blast_Score=495, Evalue=1e-141,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003699 [H]

Pfam domain/function: PF02547 Queuosine_synth [H]

EC number: 5.-.-.- [C]

Molecular weight: Translated: 38721; Mature: 38721

Theoretical pI: Translated: 5.98; Mature: 5.98

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVADFTFELPDSLIARHPLAERRSSRLLTLDGPTGALAHRQFTDLLEHLRPGDLMVFNN
CCCCCEEECCCHHHHHHCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TRVIPARLFGQKASGGKLEILVERVLDSHRVLAHVRSSKSPKPGSSILIDGGGEAEMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHH
HDALFELRFAEEVLPLLERVGRMPLPPYIDRPDEGADRERYQTVYAQRAGAVAAPTAGLH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
FDQPLLEAIAAKGVETAFVTLHVGAGTFQPVRVEQIEDHHMHSEWLEVGQDVVDAVAACR
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGGRVIAVGTTSVRSLESAARDGELKSFSGDTDIFIYPGRPFHVVDALVTNFHLPESTL
HCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHH
LMLVSAFAGYPETMAAYAAAIEHGYRFFSYGDAMFITRNPAPTAPQESAPEDHA
HHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCEEEEECCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MRVADFTFELPDSLIARHPLAERRSSRLLTLDGPTGALAHRQFTDLLEHLRPGDLMVFNN
CCCCCEEECCCHHHHHHCCHHHHHCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEECC
TRVIPARLFGQKASGGKLEILVERVLDSHRVLAHVRSSKSPKPGSSILIDGGGEAEMVAR
CEEEEHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCHHHHH
HDALFELRFAEEVLPLLERVGRMPLPPYIDRPDEGADRERYQTVYAQRAGAVAAPTAGLH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCC
FDQPLLEAIAAKGVETAFVTLHVGAGTFQPVRVEQIEDHHMHSEWLEVGQDVVDAVAACR
CCHHHHHHHHHCCCCEEEEEEEECCCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ARGGRVIAVGTTSVRSLESAARDGELKSFSGDTDIFIYPGRPFHVVDALVTNFHLPESTL
HCCCEEEEECCHHHHHHHHHHCCCCCCCCCCCCCEEEECCCCHHHHHHHHHHCCCCHHHH
LMLVSAFAGYPETMAAYAAAIEHGYRFFSYGDAMFITRNPAPTAPQESAPEDHA
HHHHHHHCCCHHHHHHHHHHHHHCCEEEEECCEEEEECCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Isomerases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA