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The map label for this gene is gnuT [H]

Identifier: 66044409

GI number: 66044409

Start: 1310315

End: 1311685

Strand: Direct

Name: gnuT [H]

Synonym: Psyr_1158

Alternate gene names: 66044409

Gene position: 1310315-1311685 (Clockwise)

Preceding gene: 66044407

Following gene: 66044411

Centisome position: 21.5

GC content: 62.87

Gene sequence:

>1371_bases
TTGGATTTGTCGTCTGCTGCCTGGGTGTTTCACGACACCCGCCTGATTATCTGCTGCCTGATTGCCATCGCCACGATCAT
CGTGCTGATCAGCGCCACCAGGTTGCCCCCCTTTCTCTCCATTCTCGTCGGCACGTTCATCGCCGGCATCGGCGCAGGTT
TGCCAGCCGAGGCTGTCGCAAAGGCCTTCAGCAAAGGCGCGGGCAGCATTCTCGGCGAGGCCGGAATCATCATCGCGCTG
GGGGCAATGCTTGGCGCGCTGATGGCCGAGTCGGGGGCTGCGGATCGCATCGCTTCCACTTTGCTCAGGCTGGGCAAGGG
CCGCACGTTGCCGTGGATAATGGCGCTGGTGGCGATGGTCATCGGCTTGCCGCTGTTCTTTGAGGTCGGTCTGGTGCTGA
TGGTGCCGATCATCTTCGTCATGGCGCGTCAGTCCGGTCAGCCGCTGCTGAAAATCGCGATTCCGGCACTGGCGGGCATG
ACCACCTTGCATGCCCTGATGCCCCCGCATCCCGGCCCGTTGATTGCGGTCAGCGCGTTGCATGCCGATCTGGGGCTGAC
CATGCTGCTGGGGTTGTGCATTGCCGTGCCGGCAGTGATTCTCGCCGGCCCGCTGTACGGCAACTGGCTATCCGGGCGCA
TGCACGTTGAAGAGCCCGCCGAGCTGGGTGAGTTGTTCAGTGCCAAGCCTGAAACCAGCCGGCAGCCTGGGTTTGGCATC
TCGCTGCTGATCATCCTTTTACCGGTGATCCTCATGCTCGGCAGCACCTTTGCCAAGATAGCGCTGGAACCTGAAAGCAA
CATCGCGCTGACCTTGAAGTTTCTAGGCGAGCCCCTGGTGGCGCTGGGCATCGCGGTGCTGGCGGCAACGGTTTGCCTGG
GCTGGGCCAACGGGCTTTCCCGTGAGCACGTCGGCGGTACATTGCGCAAAAGCCTCGCGCCCATCGCTGTGCTGTTGCTG
ACCATCGGCGCGGGTGGTGGTCTGAAGCAGACGCTGCTGGATGCCGGTGTCAGCCAGACCATCAGCAAGGTGGCAGAAGG
TGCGCACATGCCTTATCTGCTGCTCGCCTGGCTGATTGCCGTGGCACTGCGTCAGGCCACCGGGTCTGCGACCGTGGCGA
CCACGACGACGGCAGGTATCCTTGCGCCGATGATGGCCGGGCTGGCGCCGGTGCAGGCATCGCTGGTGGCGCTGGTGATC
GGTGCCGGCTCGGTGTTCTTCTGCCACGTCAACGACGCAGGCTTCTGGATGGTCCGCGAATACTTCGGCCTGCAACTCAA
GCAAACCATTTGGGTCTGGTCGGTGTTGCAGACCATCGTGTCGGTGGTCGGGCTGGTCGGGACATTGCTGTTGTGGCACT
GGTTGACGTGA

Upstream 100 bases:

>100_bases
CATTCCGGTATCGTTCGGTCAATTGAAACTAATTGTTTCTGATTCTTTCGAGGAGTGTGCTGAAAAGCACTCTGAAAAAA
CGAACGCCAGGAGGTCCGCG

Downstream 100 bases:

>100_bases
AGTGCTGCGTAATGGCCGGGAATCAGTCCGGCCCATCAACGTCGGCGTAAATCAGGCAGTGCATTCGGCTGCGGATTTGA
TGATATGCAGCAATGCCTCT

Product: gluconate transporter

Products: Proton [Cytoplasm]; gluconate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 456; Mature: 456

Protein sequence:

>456_residues
MDLSSAAWVFHDTRLIICCLIAIATIIVLISATRLPPFLSILVGTFIAGIGAGLPAEAVAKAFSKGAGSILGEAGIIIAL
GAMLGALMAESGAADRIASTLLRLGKGRTLPWIMALVAMVIGLPLFFEVGLVLMVPIIFVMARQSGQPLLKIAIPALAGM
TTLHALMPPHPGPLIAVSALHADLGLTMLLGLCIAVPAVILAGPLYGNWLSGRMHVEEPAELGELFSAKPETSRQPGFGI
SLLIILLPVILMLGSTFAKIALEPESNIALTLKFLGEPLVALGIAVLAATVCLGWANGLSREHVGGTLRKSLAPIAVLLL
TIGAGGGLKQTLLDAGVSQTISKVAEGAHMPYLLLAWLIAVALRQATGSATVATTTTAGILAPMMAGLAPVQASLVALVI
GAGSVFFCHVNDAGFWMVREYFGLQLKQTIWVWSVLQTIVSVVGLVGTLLLWHWLT

Sequences:

>Translated_456_residues
MDLSSAAWVFHDTRLIICCLIAIATIIVLISATRLPPFLSILVGTFIAGIGAGLPAEAVAKAFSKGAGSILGEAGIIIAL
GAMLGALMAESGAADRIASTLLRLGKGRTLPWIMALVAMVIGLPLFFEVGLVLMVPIIFVMARQSGQPLLKIAIPALAGM
TTLHALMPPHPGPLIAVSALHADLGLTMLLGLCIAVPAVILAGPLYGNWLSGRMHVEEPAELGELFSAKPETSRQPGFGI
SLLIILLPVILMLGSTFAKIALEPESNIALTLKFLGEPLVALGIAVLAATVCLGWANGLSREHVGGTLRKSLAPIAVLLL
TIGAGGGLKQTLLDAGVSQTISKVAEGAHMPYLLLAWLIAVALRQATGSATVATTTTAGILAPMMAGLAPVQASLVALVI
GAGSVFFCHVNDAGFWMVREYFGLQLKQTIWVWSVLQTIVSVVGLVGTLLLWHWLT
>Mature_456_residues
MDLSSAAWVFHDTRLIICCLIAIATIIVLISATRLPPFLSILVGTFIAGIGAGLPAEAVAKAFSKGAGSILGEAGIIIAL
GAMLGALMAESGAADRIASTLLRLGKGRTLPWIMALVAMVIGLPLFFEVGLVLMVPIIFVMARQSGQPLLKIAIPALAGM
TTLHALMPPHPGPLIAVSALHADLGLTMLLGLCIAVPAVILAGPLYGNWLSGRMHVEEPAELGELFSAKPETSRQPGFGI
SLLIILLPVILMLGSTFAKIALEPESNIALTLKFLGEPLVALGIAVLAATVCLGWANGLSREHVGGTLRKSLAPIAVLLL
TIGAGGGLKQTLLDAGVSQTISKVAEGAHMPYLLLAWLIAVALRQATGSATVATTTTAGILAPMMAGLAPVQASLVALVI
GAGSVFFCHVNDAGFWMVREYFGLQLKQTIWVWSVLQTIVSVVGLVGTLLLWHWLT

Specific function: Part Of The Gluconate Utilization System Gnt-I; High- Affinity Intake Of Gluconate. [C]

COG id: COG2610

COG function: function code GE; H+/gluconate symporter and related permeases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gntP permease family [H]

Homologues:

Organism=Escherichia coli, GI48994935, Length=438, Percent_Identity=40.4109589041096, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1790716, Length=429, Percent_Identity=37.2960372960373, Blast_Score=284, Evalue=9e-78,
Organism=Escherichia coli, GI1790776, Length=443, Percent_Identity=35.4401805869075, Blast_Score=252, Evalue=3e-68,
Organism=Escherichia coli, GI2367370, Length=424, Percent_Identity=37.5, Blast_Score=244, Evalue=8e-66,
Organism=Escherichia coli, GI1788707, Length=433, Percent_Identity=35.3348729792148, Blast_Score=243, Evalue=2e-65,
Organism=Escherichia coli, GI48994939, Length=435, Percent_Identity=35.1724137931034, Blast_Score=234, Evalue=1e-62,
Organism=Escherichia coli, GI1789097, Length=448, Percent_Identity=33.2589285714286, Blast_Score=223, Evalue=2e-59,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003474 [H]

Pfam domain/function: PF02447 GntP_permease [H]

EC number: NA

Molecular weight: Translated: 47339; Mature: 47339

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLSSAAWVFHDTRLIICCLIAIATIIVLISATRLPPFLSILVGTFIAGIGAGLPAEAVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
KAFSKGAGSILGEAGIIIALGAMLGALMAESGAADRIASTLLRLGKGRTLPWIMALVAMV
HHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IGLPLFFEVGLVLMVPIIFVMARQSGQPLLKIAIPALAGMTTLHALMPPHPGPLIAVSAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
HADLGLTMLLGLCIAVPAVILAGPLYGNWLSGRMHVEEPAELGELFSAKPETSRQPGFGI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
SLLIILLPVILMLGSTFAKIALEPESNIALTLKFLGEPLVALGIAVLAATVCLGWANGLS
HHHHHHHHHHHHHCCHHHHEEECCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
REHVGGTLRKSLAPIAVLLLTIGAGGGLKQTLLDAGVSQTISKVAEGAHMPYLLLAWLIA
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VALRQATGSATVATTTTAGILAPMMAGLAPVQASLVALVIGAGSVFFCHVNDAGFWMVRE
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
YFGLQLKQTIWVWSVLQTIVSVVGLVGTLLLWHWLT
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MDLSSAAWVFHDTRLIICCLIAIATIIVLISATRLPPFLSILVGTFIAGIGAGLPAEAVA
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCHHHHH
KAFSKGAGSILGEAGIIIALGAMLGALMAESGAADRIASTLLRLGKGRTLPWIMALVAMV
HHHHCCCCHHHCCCHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHH
IGLPLFFEVGLVLMVPIIFVMARQSGQPLLKIAIPALAGMTTLHALMPPHPGPLIAVSAL
HHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHH
HADLGLTMLLGLCIAVPAVILAGPLYGNWLSGRMHVEEPAELGELFSAKPETSRQPGFGI
HHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCHH
SLLIILLPVILMLGSTFAKIALEPESNIALTLKFLGEPLVALGIAVLAATVCLGWANGLS
HHHHHHHHHHHHHCCHHHHEEECCCCCEEEEHHHHCCHHHHHHHHHHHHHHHHHHHCCCC
REHVGGTLRKSLAPIAVLLLTIGAGGGLKQTLLDAGVSQTISKVAEGAHMPYLLLAWLIA
HHHCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
VALRQATGSATVATTTTAGILAPMMAGLAPVQASLVALVIGAGSVFFCHVNDAGFWMVRE
HHHHHCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCHHHHHH
YFGLQLKQTIWVWSVLQTIVSVVGLVGTLLLWHWLT
HHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; gluconate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + gluconate [Periplasm] = Proton [Cytoplasm] + gluconate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]