BacMap

Definition	Pseudomonas syringae pv. syringae B728a, complete genome.
Accession	NC_007005
Length	6,093,698

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The map label for this gene is fabG [C]

Identifier: 66044278

GI number: 66044278

Start: 1155606

End: 1156517

Strand: Direct

Name: fabG [C]

Synonym: Psyr_1025

Alternate gene names: 66044278

Gene position: 1155606-1156517 (Clockwise)

Preceding gene: 66044277

Following gene: 66044279

Centisome position: 18.96

GC content: 62.17

Gene sequence:

>912_bases
ATGAGCGAGTCAGTACGTTTTGACGATAAAGTCGTGATTGTGACCGGCGCAGGGGGTGGGTTGGGTCGGGCTCATGCGCT
GCTGTTTGCGAAACATGGTGCGCGAGTGGTGGTCAACGATCTCGGCGGTTCTGCGCATGGCGAAGGGGCCAGCGCGTCTG
CCGCCGATTGCGTCGTGGCCGAGATTCGCGCGGCAGGCGGCACTGCAATAGCCAACCATGATTCGGTGACCGAGGGCGGC
CGCATTGTGCAGCATGCGCTGGACGCATTCGGGCGCATCGATGTGCTGGTCAACAACGCTGGCATCCTGCGTGACAAGAC
CTTCGCCAACATGGAGGACGCCGACTGGGACCTGGTCTATCGCGTCCATGTCGAGGGGGCCTATAAGGTCACCCATGCCG
CCTGGCCTTATCTGCGCGAGCAGAATGACGGACGGGTGATCTTCACGTCATCGACATCCGGTATCTACGGCAACTTCGGG
CAAGCCAATTATGCGACGGCCAAGCTGGGGCTTTACGGGCTGACCCGCACCCTCGCGCTGGAAGGTCGCAAGCACCGCAT
CTTCGTCAACGCCATAGCGCCGACGGGCGGTACACGCATGACCGAGGGGCTGATTCCCGCCAATGTCTTCGAGCTGCTCA
AGCCCGAGCTGGTCAGCCCGCTGGTGGTGTACCTGTGCAGCGCACAATGCCAGAGCAGTGGTGAGCTGTTCGAAGTGGGC
GGTGGCTGGATCGGCAAGGTCCGCTGGCAGCGCAGTCAGGGAGCCTGTTTCGACCCGCAGGCAGGCTTCAGCCCGGAAGA
CGTCGCGGCGCAGTGGCAGGCGATTGGTGATTTCGCCAACGCAGCGCACCCTGCCGACACTGGCGAGGCGCTGAAGGAAA
TGATGGCCAACCTGCAGAAGTACGTTAAATAA

Upstream 100 bases:

>100_bases
TCCTTTATGGGGAATGATTCAGCACAGCAGGTGTTGATTGTGGCTGTGCAGGGCGTGGACGACACTCATCGCAACGTCTT
TCTGAGGCATGGAGCCAGCG

Downstream 100 bases:

>100_bases
TCAGCCTCTGGCAGTTGCTGCAAGACCGCGGTTATCGGTTGCGCGTGCGCGCTTATTCACGCTGACGGCTTGATTATGTG
TGAGAAGTTTCACCGAGTTA

Product: Short-chain dehydrogenase/reductase SDR

Products: (3R)-hydroxyacyl-[acyl-carrier-protein]; NADP; NADPH; Proton; beta-ketoacyl-ACP [C]

Alternate protein names: NA

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MSESVRFDDKVVIVTGAGGGLGRAHALLFAKHGARVVVNDLGGSAHGEGASASAADCVVAEIRAAGGTAIANHDSVTEGG
RIVQHALDAFGRIDVLVNNAGILRDKTFANMEDADWDLVYRVHVEGAYKVTHAAWPYLREQNDGRVIFTSSTSGIYGNFG
QANYATAKLGLYGLTRTLALEGRKHRIFVNAIAPTGGTRMTEGLIPANVFELLKPELVSPLVVYLCSAQCQSSGELFEVG
GGWIGKVRWQRSQGACFDPQAGFSPEDVAAQWQAIGDFANAAHPADTGEALKEMMANLQKYVK

Sequences:

>Translated_303_residues
MSESVRFDDKVVIVTGAGGGLGRAHALLFAKHGARVVVNDLGGSAHGEGASASAADCVVAEIRAAGGTAIANHDSVTEGG
RIVQHALDAFGRIDVLVNNAGILRDKTFANMEDADWDLVYRVHVEGAYKVTHAAWPYLREQNDGRVIFTSSTSGIYGNFG
QANYATAKLGLYGLTRTLALEGRKHRIFVNAIAPTGGTRMTEGLIPANVFELLKPELVSPLVVYLCSAQCQSSGELFEVG
GGWIGKVRWQRSQGACFDPQAGFSPEDVAAQWQAIGDFANAAHPADTGEALKEMMANLQKYVK
>Mature_302_residues
SESVRFDDKVVIVTGAGGGLGRAHALLFAKHGARVVVNDLGGSAHGEGASASAADCVVAEIRAAGGTAIANHDSVTEGGR
IVQHALDAFGRIDVLVNNAGILRDKTFANMEDADWDLVYRVHVEGAYKVTHAAWPYLREQNDGRVIFTSSTSGIYGNFGQ
ANYATAKLGLYGLTRTLALEGRKHRIFVNAIAPTGGTRMTEGLIPANVFELLKPELVSPLVVYLCSAQCQSSGELFEVGG
GWIGKVRWQRSQGACFDPQAGFSPEDVAAQWQAIGDFANAAHPADTGEALKEMMANLQKYVK

Specific function: Fatty acid biosynthesis pathway; first reduction step. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the short-chain dehydrogenases/reductases (SDR) family [H]

Homologues:

Organism=Homo sapiens, GI4504505, Length=301, Percent_Identity=56.1461794019934, Blast_Score=351, Evalue=5e-97,
Organism=Homo sapiens, GI15277342, Length=269, Percent_Identity=33.0855018587361, Blast_Score=90, Evalue=2e-18,
Organism=Homo sapiens, GI32483357, Length=260, Percent_Identity=30, Blast_Score=80, Evalue=3e-15,
Organism=Homo sapiens, GI40254992, Length=193, Percent_Identity=32.6424870466321, Blast_Score=77, Evalue=2e-14,
Organism=Homo sapiens, GI66933014, Length=190, Percent_Identity=31.5789473684211, Blast_Score=76, Evalue=4e-14,
Organism=Homo sapiens, GI40807363, Length=176, Percent_Identity=28.9772727272727, Blast_Score=71, Evalue=1e-12,
Organism=Homo sapiens, GI40254986, Length=190, Percent_Identity=32.1052631578947, Blast_Score=69, Evalue=4e-12,
Organism=Homo sapiens, GI33667109, Length=198, Percent_Identity=29.7979797979798, Blast_Score=67, Evalue=3e-11,
Organism=Homo sapiens, GI31542939, Length=190, Percent_Identity=29.4736842105263, Blast_Score=66, Evalue=4e-11,
Organism=Homo sapiens, GI5031737, Length=196, Percent_Identity=30.1020408163265, Blast_Score=65, Evalue=6e-11,
Organism=Escherichia coli, GI1787335, Length=190, Percent_Identity=33.6842105263158, Blast_Score=107, Evalue=8e-25,
Organism=Escherichia coli, GI87082100, Length=197, Percent_Identity=34.010152284264, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1788459, Length=251, Percent_Identity=31.0756972111554, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1787905, Length=265, Percent_Identity=32.0754716981132, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1789208, Length=258, Percent_Identity=30.6201550387597, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI1790717, Length=214, Percent_Identity=30.8411214953271, Blast_Score=91, Evalue=8e-20,
Organism=Escherichia coli, GI87082160, Length=187, Percent_Identity=30.4812834224599, Blast_Score=78, Evalue=6e-16,
Organism=Escherichia coli, GI1786812, Length=188, Percent_Identity=26.5957446808511, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1789057, Length=219, Percent_Identity=28.7671232876712, Blast_Score=64, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17568967, Length=298, Percent_Identity=49.6644295302013, Blast_Score=318, Evalue=2e-87,
Organism=Caenorhabditis elegans, GI25147288, Length=247, Percent_Identity=33.1983805668016, Blast_Score=107, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI17561402, Length=211, Percent_Identity=33.6492890995261, Blast_Score=87, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI17555706, Length=210, Percent_Identity=32.8571428571429, Blast_Score=86, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI72000259, Length=243, Percent_Identity=31.2757201646091, Blast_Score=85, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI17560676, Length=187, Percent_Identity=31.5508021390374, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI17559104, Length=195, Percent_Identity=29.7435897435897, Blast_Score=82, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71982365, Length=202, Percent_Identity=30.6930693069307, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17562990, Length=199, Percent_Identity=29.1457286432161, Blast_Score=80, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17563726, Length=248, Percent_Identity=28.6290322580645, Blast_Score=79, Evalue=3e-15,
Organism=Caenorhabditis elegans, GI193204405, Length=198, Percent_Identity=30.8080808080808, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17560332, Length=215, Percent_Identity=29.7674418604651, Blast_Score=79, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17567345, Length=174, Percent_Identity=28.735632183908, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17508895, Length=249, Percent_Identity=25.7028112449799, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17564282, Length=188, Percent_Identity=27.6595744680851, Blast_Score=76, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI17562904, Length=201, Percent_Identity=29.3532338308458, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17560150, Length=199, Percent_Identity=29.1457286432161, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI17562910, Length=215, Percent_Identity=27.906976744186, Blast_Score=74, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI17538480, Length=197, Percent_Identity=30.4568527918782, Blast_Score=74, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17558628, Length=198, Percent_Identity=33.3333333333333, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17562908, Length=215, Percent_Identity=28.3720930232558, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI17531453, Length=204, Percent_Identity=30.3921568627451, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71994604, Length=192, Percent_Identity=30.7291666666667, Blast_Score=72, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI193203117, Length=222, Percent_Identity=27.9279279279279, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI193203115, Length=217, Percent_Identity=28.5714285714286, Blast_Score=72, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17538486, Length=208, Percent_Identity=25.9615384615385, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI71994600, Length=192, Percent_Identity=30.2083333333333, Blast_Score=71, Evalue=6e-13,
Organism=Caenorhabditis elegans, GI17551412, Length=195, Percent_Identity=28.7179487179487, Blast_Score=70, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17560220, Length=200, Percent_Identity=27, Blast_Score=66, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17562906, Length=200, Percent_Identity=28, Blast_Score=66, Evalue=2e-11,
Organism=Saccharomyces cerevisiae, GI6322861, Length=291, Percent_Identity=49.1408934707904, Blast_Score=300, Evalue=2e-82,
Organism=Drosophila melanogaster, GI18859837, Length=305, Percent_Identity=48.5245901639344, Blast_Score=309, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24639444, Length=252, Percent_Identity=32.1428571428571, Blast_Score=94, Evalue=1e-19,
Organism=Drosophila melanogaster, GI23397609, Length=188, Percent_Identity=34.0425531914894, Blast_Score=88, Evalue=9e-18,
Organism=Drosophila melanogaster, GI21357041, Length=202, Percent_Identity=35.1485148514851, Blast_Score=87, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24644339, Length=190, Percent_Identity=31.0526315789474, Blast_Score=84, Evalue=1e-16,
Organism=Drosophila melanogaster, GI28571526, Length=191, Percent_Identity=33.5078534031414, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24649181, Length=190, Percent_Identity=30, Blast_Score=78, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24640492, Length=178, Percent_Identity=28.6516853932584, Blast_Score=76, Evalue=4e-14,
Organism=Drosophila melanogaster, GI21357673, Length=244, Percent_Identity=28.2786885245902, Blast_Score=74, Evalue=8e-14,
Organism=Drosophila melanogaster, GI21355319, Length=200, Percent_Identity=28.5, Blast_Score=71, Evalue=1e-12,
Organism=Drosophila melanogaster, GI24581726, Length=218, Percent_Identity=27.5229357798165, Blast_Score=68, Evalue=8e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002198
- InterPro:   IPR002347
- InterPro:   IPR016040
- InterPro:   IPR020904 [H]

Pfam domain/function: PF00106 adh_short [H]

EC number: 1.1.1.100 [C]

Molecular weight: Translated: 32207; Mature: 32075

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSESVRFDDKVVIVTGAGGGLGRAHALLFAKHGARVVVNDLGGSAHGEGASASAADCVVA
CCCCCCCCCEEEEEEECCCCCCHHHEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHH
EIRAAGGTAIANHDSVTEGGRIVQHALDAFGRIDVLVNNAGILRDKTFANMEDADWDLVY
HHHHCCCCEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCCCCEEEE
RVHVEGAYKVTHAAWPYLREQNDGRVIFTSSTSGIYGNFGQANYATAKLGLYGLTRTLAL
EEEECCEEEEEHHHCCHHEECCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHEEEEE
EGRKHRIFVNAIAPTGGTRMTEGLIPANVFELLKPELVSPLVVYLCSAQCQSSGELFEVG
CCCCEEEEEEEECCCCCCCHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEC
GGWIGKVRWQRSQGACFDPQAGFSPEDVAAQWQAIGDFANAAHPADTGEALKEMMANLQK
CCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YVK
HCC
>Mature Secondary Structure 
SESVRFDDKVVIVTGAGGGLGRAHALLFAKHGARVVVNDLGGSAHGEGASASAADCVVA
CCCCCCCCEEEEEEECCCCCCHHHEEEEECCCCEEEEECCCCCCCCCCCCCCHHHHHHH
EIRAAGGTAIANHDSVTEGGRIVQHALDAFGRIDVLVNNAGILRDKTFANMEDADWDLVY
HHHHCCCCEEECCCCCCCCHHHHHHHHHHCCEEEEEECCCCEEECCCCCCCCCCCCEEEE
RVHVEGAYKVTHAAWPYLREQNDGRVIFTSSTSGIYGNFGQANYATAKLGLYGLTRTLAL
EEEECCEEEEEHHHCCHHEECCCCEEEEEECCCCCCCCCCCCCCEEEHHHHHHHHEEEEE
EGRKHRIFVNAIAPTGGTRMTEGLIPANVFELLKPELVSPLVVYLCSAQCQSSGELFEVG
CCCCEEEEEEEECCCCCCCHHCCCCCHHHHHHHCHHHHHHHHHHHHHHHHCCCCCEEEEC
GGWIGKVRWQRSQGACFDPQAGFSPEDVAAQWQAIGDFANAAHPADTGEALKEMMANLQK
CCEEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHH
YVK
HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NADPH [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Oxoacyl-[acyl-carrier-protein]; NADPH; D-3-hydroxy-acyl-ACP; NADP [C]

Specific reaction: Oxoacyl-[acyl-carrier-protein] + NADPH = (3R)-hydroxyacyl-[acyl-carrier-protein] + NADP+ D-3-hydroxy-acyl-ACP + NADP = NADPH + Proton + beta-ketoacyl-ACP [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]