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The map label for this gene is folE [H]

Identifier: 66044273

GI number: 66044273

Start: 1151982

End: 1152545

Strand: Reverse

Name: folE [H]

Synonym: Psyr_1020

Alternate gene names: 66044273

Gene position: 1152545-1151982 (Counterclockwise)

Preceding gene: 66044274

Following gene: 66044272

Centisome position: 18.91

GC content: 56.03

Gene sequence:

>564_bases
ATGAGTTCGTCCCTGCCCCAGCACTATCGAGACATTCTGCTCGGCCTTGGTGAAGATCCAGACCGCGAAGGTCTGCTGGA
CACGCCGAAACGCGCTTCCAAAGCCATGCAGTACCTGTGTCATGGCTACACCCAGAGCGTCGAAGAAATCGTCAATGGCG
CGCTGTTCGCCTCCGATAACGATGAGATGGTCATCGTCCAGAACATCGAGTTGTACTCGCTTTGCGAGCACCACCTGCTG
CCGTTCATAGGCAAGGCGCATGTCGCCTACATTCCGACCGGCAAGGTGCTGGGTCTGTCGAAGATCGCACGCATCGTCGA
CATGTTTGCCCGCCGTCTGCAGATTCAGGAAAATCTGACCAAACAGATTGCCGATGCGATTCAGAAAGTCACCGGAGCCG
CCGGTGTAGCGGTGGTCATCGAAGCCCAGCACATGTGCATGATGATGCGCGGCGTCGAAAAGCAGAATTCGACCATGAAT
ACGTCAGTGATGCTCGGCGCCTTCCGTGACTCGAGCACCACGCGCATGGAGTTTCTGCAATTGATCGGACGGAGCAGACA
ATAA

Upstream 100 bases:

>100_bases
GAGCCTGCGTTATCTTCTGGACAGCACCTATGTCACCGGCACAACACTGACCGTAAACGGCGGACGGCACGTCAAGTAAG
CCGCCCCGAGGATCATTCTG

Downstream 100 bases:

>100_bases
TGGCAAGACTCGAACCTGGCACCGCACGCATCCGCGTCAAGGACCTGCGCCTGCGCACGTTCATCGGCATCAACGAGGAC
GAAATCCTCAACAAACAGGA

Product: GTP cyclohydrolase I

Products: NA

Alternate protein names: GTP cyclohydrolase I 1; GTP-CH-I 1 [H]

Number of amino acids: Translated: 187; Mature: 186

Protein sequence:

>187_residues
MSSSLPQHYRDILLGLGEDPDREGLLDTPKRASKAMQYLCHGYTQSVEEIVNGALFASDNDEMVIVQNIELYSLCEHHLL
PFIGKAHVAYIPTGKVLGLSKIARIVDMFARRLQIQENLTKQIADAIQKVTGAAGVAVVIEAQHMCMMMRGVEKQNSTMN
TSVMLGAFRDSSTTRMEFLQLIGRSRQ

Sequences:

>Translated_187_residues
MSSSLPQHYRDILLGLGEDPDREGLLDTPKRASKAMQYLCHGYTQSVEEIVNGALFASDNDEMVIVQNIELYSLCEHHLL
PFIGKAHVAYIPTGKVLGLSKIARIVDMFARRLQIQENLTKQIADAIQKVTGAAGVAVVIEAQHMCMMMRGVEKQNSTMN
TSVMLGAFRDSSTTRMEFLQLIGRSRQ
>Mature_186_residues
SSSLPQHYRDILLGLGEDPDREGLLDTPKRASKAMQYLCHGYTQSVEEIVNGALFASDNDEMVIVQNIELYSLCEHHLLP
FIGKAHVAYIPTGKVLGLSKIARIVDMFARRLQIQENLTKQIADAIQKVTGAAGVAVVIEAQHMCMMMRGVEKQNSTMNT
SVMLGAFRDSSTTRMEFLQLIGRSRQ

Specific function: Tetrahydrofolate biosynthesis; first step. [C]

COG id: COG0302

COG function: function code H; GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family [H]

Homologues:

Organism=Homo sapiens, GI66932968, Length=174, Percent_Identity=57.4712643678161, Blast_Score=225, Evalue=2e-59,
Organism=Homo sapiens, GI4503949, Length=174, Percent_Identity=57.4712643678161, Blast_Score=225, Evalue=2e-59,
Organism=Homo sapiens, GI66932970, Length=144, Percent_Identity=52.7777777777778, Blast_Score=173, Evalue=6e-44,
Organism=Homo sapiens, GI66932972, Length=134, Percent_Identity=55.9701492537313, Blast_Score=173, Evalue=7e-44,
Organism=Escherichia coli, GI1788476, Length=186, Percent_Identity=40.8602150537634, Blast_Score=120, Evalue=5e-29,
Organism=Caenorhabditis elegans, GI17560486, Length=181, Percent_Identity=58.5635359116022, Blast_Score=228, Evalue=1e-60,
Organism=Saccharomyces cerevisiae, GI6321706, Length=176, Percent_Identity=57.9545454545455, Blast_Score=213, Evalue=1e-56,
Organism=Drosophila melanogaster, GI24656791, Length=175, Percent_Identity=60, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656782, Length=174, Percent_Identity=60.3448275862069, Blast_Score=218, Evalue=2e-57,
Organism=Drosophila melanogaster, GI24656796, Length=175, Percent_Identity=60, Blast_Score=218, Evalue=3e-57,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001474
- InterPro:   IPR020602
- InterPro:   IPR018234 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =3.5.4.16 [H]

Molecular weight: Translated: 20812; Mature: 20681

Theoretical pI: Translated: 7.13; Mature: 7.13

Prosite motif: PS00859 GTP_CYCLOHYDROL_1_1 ; PS00860 GTP_CYCLOHYDROL_1_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
5.9 %Met     (Translated Protein)
7.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
5.4 %Met     (Mature Protein)
7.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSSLPQHYRDILLGLGEDPDREGLLDTPKRASKAMQYLCHGYTQSVEEIVNGALFASDN
CCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
DEMVIVQNIELYSLCEHHLLPFIGKAHVAYIPTGKVLGLSKIARIVDMFARRLQIQENLT
CCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQIADAIQKVTGAAGVAVVIEAQHMCMMMRGVEKQNSTMNTSVMLGAFRDSSTTRMEFLQ
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHH
LIGRSRQ
HHCCCCC
>Mature Secondary Structure 
SSSLPQHYRDILLGLGEDPDREGLLDTPKRASKAMQYLCHGYTQSVEEIVNGALFASDN
CCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
DEMVIVQNIELYSLCEHHLLPFIGKAHVAYIPTGKVLGLSKIARIVDMFARRLQIQENLT
CCEEEEECCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHH
KQIADAIQKVTGAAGVAVVIEAQHMCMMMRGVEKQNSTMNTSVMLGAFRDSSTTRMEFLQ
HHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHCCCCCCHHHEEEECCCCCHHHHHHHH
LIGRSRQ
HHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]