Click here to switch to the map view.

The map label for this gene is rutB [H]

Identifier: 66044251

GI number: 66044251

Start: 1131651

End: 1132388

Strand: Reverse

Name: rutB [H]

Synonym: Psyr_0998

Alternate gene names: 66044251

Gene position: 1132388-1131651 (Counterclockwise)

Preceding gene: 66044252

Following gene: 66044250

Centisome position: 18.58

GC content: 62.74

Gene sequence:

>738_bases
ATGACAACCCCGACAACCGTCGCAGGCGTTGACCTGCCGGACCTGCAGCCCGCCCGCGAGCTGCCCGCCCGTCCCGAGCC
GCTGCGCATGAAACCCGGCGAAACCGCGCTGGTGGTGGTCGACATGCAGAACGCCTACGCCTCGCTCGGTGGGTATCTGG
ACCTGGCCGGGTTCGATGTCAGCAGCACCGGGCCGGTCATCGCCAATATCAACAAGGCCTGTGCCGCCGCGCGTGCAGCA
GGCATTCCGGTGATCTTTTTCCAGAATGGCTGGGACCCGGCCTATGTCGAAGCCGGTGGCCCCGGTTCACCGAACTGGCA
CAAGTCCAACGCCCTGAAAACCATGCGCAAGCGCCCGGAACTCGAAGGGCAACTGCTGGCCAAGGGCGGTTGGGATTACC
AGCTGGTCGACGAACTGACGCCACAGCCTGGCGACATCGTGGTGCCGAAAATCCGCTACAGCGGCTTCTTCAACTCCAGT
TTCGACAGCGTGCTGCGCAGCCGCGGCATTCGCAATCTGGTGTTCACCGGCATCGCCACCAATGTCTGTGTCGAGTCGAC
CCTGCGCGACGGCTTTCACCTGGAATATTTCGGCGTGGTGCTGGCTGACGCGACCCATCAGGCAGGCCCCGAATTCGCTC
AGCAAGCCGCGCTGTTCAACATCGAAACCTTCTTTGGCTGGGTTTCCAGCGTCGATGACTTCTGCACCACTTTCAGCCCT
GTCGGGCAACCTTCGTGA

Upstream 100 bases:

>100_bases
CGTTCGATGATTTCGTCAAAGGGGTCGAGGATTTCGGCGAGAAGATCCAGCCACTGATGACCAGCCGCAAGCACATCGCG
CAGCTCAAGGAGGTGGTCTG

Downstream 100 bases:

>100_bases
GGACACCAGCATGACCAAGAAAGCGATCATCCCTGCCGGCACCAGCAAACCCATCGCGCCGTTCGTACCGGGCTCCATGG
CCGACGGCGTGCTCTATGTC

Product: isochorismatase hydrolase

Products: NA

Alternate protein names: Ureidoacrylate amidohydrolase [H]

Number of amino acids: Translated: 245; Mature: 244

Protein sequence:

>245_residues
MTTPTTVAGVDLPDLQPARELPARPEPLRMKPGETALVVVDMQNAYASLGGYLDLAGFDVSSTGPVIANINKACAAARAA
GIPVIFFQNGWDPAYVEAGGPGSPNWHKSNALKTMRKRPELEGQLLAKGGWDYQLVDELTPQPGDIVVPKIRYSGFFNSS
FDSVLRSRGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALFNIETFFGWVSSVDDFCTTFSP
VGQPS

Sequences:

>Translated_245_residues
MTTPTTVAGVDLPDLQPARELPARPEPLRMKPGETALVVVDMQNAYASLGGYLDLAGFDVSSTGPVIANINKACAAARAA
GIPVIFFQNGWDPAYVEAGGPGSPNWHKSNALKTMRKRPELEGQLLAKGGWDYQLVDELTPQPGDIVVPKIRYSGFFNSS
FDSVLRSRGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALFNIETFFGWVSSVDDFCTTFSP
VGQPS
>Mature_244_residues
TTPTTVAGVDLPDLQPARELPARPEPLRMKPGETALVVVDMQNAYASLGGYLDLAGFDVSSTGPVIANINKACAAARAAG
IPVIFFQNGWDPAYVEAGGPGSPNWHKSNALKTMRKRPELEGQLLAKGGWDYQLVDELTPQPGDIVVPKIRYSGFFNSSF
DSVLRSRGIRNLVFTGIATNVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALFNIETFFGWVSSVDDFCTTFSPV
GQPS

Specific function: In vivo, quickly hydrolyzes the ureidoacrylate peracid to avoid toxicity, but can also hydrolyzes ureidoacrylate that is formed spontaneously from ureidoacrylate peracid. One of the products of hydrolysis, carbamate, hydrolyzes spontaneously, thereby rele

COG id: COG1335

COG function: function code Q; Amidases related to nicotinamidase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isochorismatase family. RutB subfamily [H]

Homologues:

Organism=Escherichia coli, GI87081820, Length=220, Percent_Identity=78.1818181818182, Blast_Score=362, Evalue=1e-102,
Organism=Escherichia coli, GI87081992, Length=184, Percent_Identity=30.4347826086957, Blast_Score=68, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019916
- InterPro:   IPR000868 [H]

Pfam domain/function: PF00857 Isochorismatase [H]

EC number: NA

Molecular weight: Translated: 26385; Mature: 26254

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTPTTVAGVDLPDLQPARELPARPEPLRMKPGETALVVVDMQNAYASLGGYLDLAGFDV
CCCCCEEECCCCCCCCCHHHCCCCCCCCEECCCCEEEEEEECCHHHHHHCCEEEEECCCC
SSTGPVIANINKACAAARAAGIPVIFFQNGWDPAYVEAGGPGSPNWHKSNALKTMRKRPE
CCCCCEEECCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCC
LEGQLLAKGGWDYQLVDELTPQPGDIVVPKIRYSGFFNSSFDSVLRSRGIRNLVFTGIAT
CCCEEEECCCCCEEHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHH
NVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALFNIETFFGWVSSVDDFCTTFSP
HHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGQPS
CCCCC
>Mature Secondary Structure 
TTPTTVAGVDLPDLQPARELPARPEPLRMKPGETALVVVDMQNAYASLGGYLDLAGFDV
CCCCEEECCCCCCCCCHHHCCCCCCCCEECCCCEEEEEEECCHHHHHHCCEEEEECCCC
SSTGPVIANINKACAAARAAGIPVIFFQNGWDPAYVEAGGPGSPNWHKSNALKTMRKRPE
CCCCCEEECCHHHHHHHHHCCCCEEEEECCCCEEEEECCCCCCCCCCHHHHHHHHHHCCC
LEGQLLAKGGWDYQLVDELTPQPGDIVVPKIRYSGFFNSSFDSVLRSRGIRNLVFTGIAT
CCCEEEECCCCCEEHHHHCCCCCCCEEEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHHH
NVCVESTLRDGFHLEYFGVVLADATHQAGPEFAQQAALFNIETFFGWVSSVDDFCTTFSP
HHHHHHHHCCCCCEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
VGQPS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA