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The map label for this gene is rutD

Identifier: 66044249

GI number: 66044249

Start: 1130436

End: 1131215

Strand: Reverse

Name: rutD

Synonym: Psyr_0996

Alternate gene names: 66044249

Gene position: 1131215-1130436 (Counterclockwise)

Preceding gene: 66044250

Following gene: 66044248

Centisome position: 18.56

GC content: 63.21

Gene sequence:

>780_bases
ATGTTCCATGAGTTTCATGCCTGCCAGCATGCCGATGCACCGACCCTGGTGCTCAGTTCCGGGCTGGGCGGGTCTGGCCG
CTACTGGGCGGATGACCTGACGCTGCTGACCCGCGATTACCATGTGCTGGTCTATGACCATGCCGGAACCGGGCGCAGCC
CTGCGGTGTTGCCGGCGGATTACTCGATCCGGCACATGGCTATCGAGTTGCTGGCCCTGCTCGACTCACTCGATATCCAG
CGCTGCCATTTCATGGGCCATGCACTGGGCGGCCTGGTGGGTCTGGAACTGGCCCTGCTGCGCCCGGAACTGCTGCACAG
CCAGGTACTGATCAATGCCTGGAGCAGTCCGAACCCGCATAGCGCGCGCTGCTTTTCAGTGCGCAAAAAACTGCTGTTGA
ACAGCGGACCGGAGGCGTACGTACAGGCTCAGGCGCTGTTCCTCTACCCGGCAGACTGGATCGCCGCCAACGGCCCGCGG
CTGGCCGACGACGAAGCCCACGCTCTGGCGCATTTCCCCGACACCGACAACCTGCTGCGGCGCATCCATGCGCTGGAAAC
CTTCGACGTGTCGGCCGAGCTTTCACGCATTCACACCCCGACCCTGCTGATTGCCAACCGCGACGACATGCTGGTGCCCT
GGCAGCAATCCCGGCACCTGGCCAACGCCCTGCCGAATGCAACGCTGGTGCTGCTGGAATATGGCGGCCATGCCTCTAAC
ATTACCGATCCACTGCCCTTCCAACGCGCCCTGCGCGCTTTTCTCAGCACACAACCCTGA

Upstream 100 bases:

>100_bases
GCGGCCTGGTCAAACCCGAGGCGCTGATCGAAATCGCCAGCATCGCCCACATCGGCTGAATCGCGACCGTGGGCAGTTGA
CGCCCACCCGGAGAAAACCC

Downstream 100 bases:

>100_bases
CACACAACCCTGAAAGGACTCTTCCAATGCCTACCTCCTCTGCAACTCCCGCCATTCTGCCCATCGCCGCTGTCAGCCAG
CTGGCGTTTCGCGATGCGAT

Product: Alpha/beta hydrolase fold

Products: NA

Alternate protein names: Aminohydrolase

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MFHEFHACQHADAPTLVLSSGLGGSGRYWADDLTLLTRDYHVLVYDHAGTGRSPAVLPADYSIRHMAIELLALLDSLDIQ
RCHFMGHALGGLVGLELALLRPELLHSQVLINAWSSPNPHSARCFSVRKKLLLNSGPEAYVQAQALFLYPADWIAANGPR
LADDEAHALAHFPDTDNLLRRIHALETFDVSAELSRIHTPTLLIANRDDMLVPWQQSRHLANALPNATLVLLEYGGHASN
ITDPLPFQRALRAFLSTQP

Sequences:

>Translated_259_residues
MFHEFHACQHADAPTLVLSSGLGGSGRYWADDLTLLTRDYHVLVYDHAGTGRSPAVLPADYSIRHMAIELLALLDSLDIQ
RCHFMGHALGGLVGLELALLRPELLHSQVLINAWSSPNPHSARCFSVRKKLLLNSGPEAYVQAQALFLYPADWIAANGPR
LADDEAHALAHFPDTDNLLRRIHALETFDVSAELSRIHTPTLLIANRDDMLVPWQQSRHLANALPNATLVLLEYGGHASN
ITDPLPFQRALRAFLSTQP
>Mature_259_residues
MFHEFHACQHADAPTLVLSSGLGGSGRYWADDLTLLTRDYHVLVYDHAGTGRSPAVLPADYSIRHMAIELLALLDSLDIQ
RCHFMGHALGGLVGLELALLRPELLHSQVLINAWSSPNPHSARCFSVRKKLLLNSGPEAYVQAQALFLYPADWIAANGPR
LADDEAHALAHFPDTDNLLRRIHALETFDVSAELSRIHTPTLLIANRDDMLVPWQQSRHLANALPNATLVLLEYGGHASN
ITDPLPFQRALRAFLSTQP

Specific function: May increase the rate of spontaneous hydrolysis of aminoacrylate to malonic semialdehyde. Required to remove a toxic intermediate produce in the pyrimidine nitrogen degradation

COG id: COG0596

COG function: function code R; Predicted hydrolases or acyltransferases (alpha/beta hydrolase superfamily)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase superfamily. Hydrolase RutD family

Homologues:

Organism=Escherichia coli, GI1787244, Length=238, Percent_Identity=48.7394957983193, Blast_Score=226, Evalue=1e-60,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RUTD_PSEU2 (Q4ZXS0)

Other databases:

- EMBL:   CP000075
- RefSeq:   YP_234090.1
- ProteinModelPortal:   Q4ZXS0
- STRING:   Q4ZXS0
- GeneID:   3366490
- GenomeReviews:   CP000075_GR
- KEGG:   psb:Psyr_0996
- NMPDR:   fig|205918.4.peg.1315
- eggNOG:   COG0596
- HOGENOM:   HBG748003
- OMA:   YPADWIA
- ProtClustDB:   CLSK912658
- BioCyc:   PSYR205918:PSYR_0996-MONOMER
- HAMAP:   MF_00832
- InterPro:   IPR000073
- InterPro:   IPR019913
- PRINTS:   PR00111
- TIGRFAMs:   TIGR03611

Pfam domain/function: PF00561 Abhydrolase_1

EC number: NA

Molecular weight: Translated: 28620; Mature: 28620

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFHEFHACQHADAPTLVLSSGLGGSGRYWADDLTLLTRDYHVLVYDHAGTGRSPAVLPAD
CCCCCCCHHCCCCCEEEEECCCCCCCCEECCCEEEEECCEEEEEEECCCCCCCCEEECCC
YSIRHMAIELLALLDSLDIQRCHFMGHALGGLVGLELALLRPELLHSQVLINAWSSPNPH
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEECCCCCCCC
SARCFSVRKKLLLNSGPEAYVQAQALFLYPADWIAANGPRLADDEAHALAHFPDTDNLLR
CHHHHHHHHHHHHCCCCHHHEEEEEEEEECCHHHCCCCCCCCCCCCHHEEECCCHHHHHH
RIHALETFDVSAELSRIHTPTLLIANRDDMLVPWQQSRHLANALPNATLVLLEYGGHASN
HHHHHHHCCCHHHHHHCCCCEEEEECCCCEECCHHHHHHHHHHCCCCEEEEEEECCCCCC
ITDPLPFQRALRAFLSTQP
CCCCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFHEFHACQHADAPTLVLSSGLGGSGRYWADDLTLLTRDYHVLVYDHAGTGRSPAVLPAD
CCCCCCCHHCCCCCEEEEECCCCCCCCEECCCEEEEECCEEEEEEECCCCCCCCEEECCC
YSIRHMAIELLALLDSLDIQRCHFMGHALGGLVGLELALLRPELLHSQVLINAWSSPNPH
CHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHEEECCCCCCCC
SARCFSVRKKLLLNSGPEAYVQAQALFLYPADWIAANGPRLADDEAHALAHFPDTDNLLR
CHHHHHHHHHHHHCCCCHHHEEEEEEEEECCHHHCCCCCCCCCCCCHHEEECCCHHHHHH
RIHALETFDVSAELSRIHTPTLLIANRDDMLVPWQQSRHLANALPNATLVLLEYGGHASN
HHHHHHHCCCHHHHHHCCCCEEEEECCCCEECCHHHHHHHHHHCCCCEEEEEEECCCCCC
ITDPLPFQRALRAFLSTQP
CCCCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA