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The map label for this gene is prfA [H]

Identifier: 66044202

GI number: 66044202

Start: 1084901

End: 1085983

Strand: Direct

Name: prfA [H]

Synonym: Psyr_0949

Alternate gene names: 66044202

Gene position: 1084901-1085983 (Clockwise)

Preceding gene: 66044201

Following gene: 66044203

Centisome position: 17.8

GC content: 60.02

Gene sequence:

>1083_bases
ATGAAAGCTTCACTGCTCAATAAGCTGGATGTGCTCAGCGACCGTTTCGAGGAACTGACCGCGCTGCTCGGCGACGCGGA
AGTCATCAGCGACCAGACACGCTTTCGCGCCTATTCCCGTGAATACGCTGAAGTGGAGCCAGTCGTTGCGCTCTACGCGC
AGTTGCTCAGGGTGCAGGGCGACCTGGAGGGCGCACAGGCGCTGCTCAAGGACAGCGATCCGGACATGCGCGAAATGGCC
GTCGAGGAAGTTCGCGAAACCAAACAGCAACTGGTCGAGCTGGAAGCGCAATTGCAGCGCATGCTGTTGCCCAAGGACCC
GAACGACGGTCGTAACGTGTTTCTGGAAATCCGCGCAGGCACCGGCGGCGACGAAGCGGCGATCTTCTCCGGCGACCTGT
TCCGCATGTATTCGCGTTACGCCGAGCGGCGTGGCTGGCGGGTCGAGATTCTTTCCGAAAACGAAGGCGAGCATGGCGGC
TACAAGGAAGTCATTGCACGTGTCGAGGGTGACAGCGTCTACGGCAAGCTGAAATTCGAGTCCGGTGCGCACCGTGTGCA
GCGTGTTCCGGAGACCGAATCCCAGGGCCGCATCCATACCTCAGCCTGTACGGTTGCGGTATTGCCGGAGCCTGACGAGC
AGCAGGCGATCGAGATCAACCCAGCCGACCTGCGGGTCGACACCTATCGTTCTTCGGGCGCTGGTGGACAGCACGTCAAC
AAAACCGACTCGGCGATCCGCATTACCCACTTGCCGTCGGGTATTGTCGTCGAGTGCCAGGAAGAGCGTTCGCAGCACAA
GAACAGAGCCCGGGCAATGTCCTGGCTATCTGCCAAACTGAATGATCAGCAGACCAGCGCGGCGGCCAATGCCATTGCCA
GCGAACGCAAGCTGCTGGTCGGCTCGGGCGATCGGTCCGAGCGCATACGTACGTATAACTTCCCTCAGGGGCGAGTGACG
GATCACCGCGTGAACCTGACTTTGTATTCGCTGGATGAAGTTCTGGCGGGCGGGGTCGATGCAGTGATAGAGCCGCTACT
CGCTGAATATCAGGCTGATCAACTTGCGGCACTGGGTGAGTAA

Upstream 100 bases:

>100_bases
TGCAGCTCAAGAAGCTGTCTGCCGAAGGTCGCGTCGATGCGCTGGCCATGGCCCAGGAACTTTTTGCCCTCGGTGAGGGC
TCGACGGATAAACCCCCGCA

Downstream 100 bases:

>100_bases
ATGACCATCATCGCAAGTGTACTCAGAAGCGCCGAGCTTCCCGATTCACCGACTGCACGGCTTGATGCCGAGCTTCTGCT
GGCGGCGGCGCTGGGCAAAC

Product: peptide chain release factor 1

Products: NA

Alternate protein names: RF-1 [H]

Number of amino acids: Translated: 360; Mature: 360

Protein sequence:

>360_residues
MKASLLNKLDVLSDRFEELTALLGDAEVISDQTRFRAYSREYAEVEPVVALYAQLLRVQGDLEGAQALLKDSDPDMREMA
VEEVRETKQQLVELEAQLQRMLLPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGG
YKEVIARVEGDSVYGKLKFESGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQQAIEINPADLRVDTYRSSGAGGQHVN
KTDSAIRITHLPSGIVVECQEERSQHKNRARAMSWLSAKLNDQQTSAAANAIASERKLLVGSGDRSERIRTYNFPQGRVT
DHRVNLTLYSLDEVLAGGVDAVIEPLLAEYQADQLAALGE

Sequences:

>Translated_360_residues
MKASLLNKLDVLSDRFEELTALLGDAEVISDQTRFRAYSREYAEVEPVVALYAQLLRVQGDLEGAQALLKDSDPDMREMA
VEEVRETKQQLVELEAQLQRMLLPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGG
YKEVIARVEGDSVYGKLKFESGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQQAIEINPADLRVDTYRSSGAGGQHVN
KTDSAIRITHLPSGIVVECQEERSQHKNRARAMSWLSAKLNDQQTSAAANAIASERKLLVGSGDRSERIRTYNFPQGRVT
DHRVNLTLYSLDEVLAGGVDAVIEPLLAEYQADQLAALGE
>Mature_360_residues
MKASLLNKLDVLSDRFEELTALLGDAEVISDQTRFRAYSREYAEVEPVVALYAQLLRVQGDLEGAQALLKDSDPDMREMA
VEEVRETKQQLVELEAQLQRMLLPKDPNDGRNVFLEIRAGTGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGG
YKEVIARVEGDSVYGKLKFESGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQQAIEINPADLRVDTYRSSGAGGQHVN
KTDSAIRITHLPSGIVVECQEERSQHKNRARAMSWLSAKLNDQQTSAAANAIASERKLLVGSGDRSERIRTYNFPQGRVT
DHRVNLTLYSLDEVLAGGVDAVIEPLLAEYQADQLAALGE

Specific function: Peptide chain release factor 1 directs the termination of translation in response to the peptide chain termination codons UAG and UAA [H]

COG id: COG0216

COG function: function code J; Protein chain release factor A

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI166795303, Length=296, Percent_Identity=46.2837837837838, Blast_Score=278, Evalue=7e-75,
Organism=Homo sapiens, GI34577120, Length=366, Percent_Identity=39.8907103825137, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI166795305, Length=193, Percent_Identity=46.1139896373057, Blast_Score=189, Evalue=4e-48,
Organism=Escherichia coli, GI1787462, Length=360, Percent_Identity=66.6666666666667, Blast_Score=484, Evalue=1e-138,
Organism=Escherichia coli, GI2367172, Length=341, Percent_Identity=39.8826979472141, Blast_Score=226, Evalue=2e-60,
Organism=Caenorhabditis elegans, GI17542784, Length=297, Percent_Identity=38.3838383838384, Blast_Score=199, Evalue=3e-51,
Organism=Saccharomyces cerevisiae, GI6321295, Length=316, Percent_Identity=42.7215189873418, Blast_Score=245, Evalue=1e-65,
Organism=Drosophila melanogaster, GI19921226, Length=331, Percent_Identity=39.5770392749245, Blast_Score=226, Evalue=3e-59,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005139
- InterPro:   IPR000352
- InterPro:   IPR004373 [H]

Pfam domain/function: PF03462 PCRF; PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 40025; Mature: 40025

Theoretical pI: Translated: 4.73; Mature: 4.73

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKASLLNKLDVLSDRFEELTALLGDAEVISDQTRFRAYSREYAEVEPVVALYAQLLRVQG
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLEGAQALLKDSDPDMREMAVEEVRETKQQLVELEAQLQRMLLPKDPNDGRNVFLEIRAG
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
TGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGGYKEVIARVEGDSVYGKLKFE
CCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEC
SGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQQAIEINPADLRVDTYRSSGAGGQHVN
CCHHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCC
KTDSAIRITHLPSGIVVECQEERSQHKNRARAMSWLSAKLNDQQTSAAANAIASERKLLV
CCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEE
GSGDRSERIRTYNFPQGRVTDHRVNLTLYSLDEVLAGGVDAVIEPLLAEYQADQLAALGE
ECCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKASLLNKLDVLSDRFEELTALLGDAEVISDQTRFRAYSREYAEVEPVVALYAQLLRVQG
CCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
DLEGAQALLKDSDPDMREMAVEEVRETKQQLVELEAQLQRMLLPKDPNDGRNVFLEIRAG
CHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEC
TGGDEAAIFSGDLFRMYSRYAERRGWRVEILSENEGEHGGYKEVIARVEGDSVYGKLKFE
CCCCCCEEECHHHHHHHHHHHHCCCCEEEEEECCCCCCCCHHHHHHHCCCCCEEEEEEEC
SGAHRVQRVPETESQGRIHTSACTVAVLPEPDEQQAIEINPADLRVDTYRSSGAGGQHVN
CCHHHHHCCCCCCCCCEEEECEEEEEECCCCCCCCEEEECCCCEEEEECCCCCCCCCCCC
KTDSAIRITHLPSGIVVECQEERSQHKNRARAMSWLSAKLNDQQTSAAANAIASERKLLV
CCCCEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCEEEE
GSGDRSERIRTYNFPQGRVTDHRVNLTLYSLDEVLAGGVDAVIEPLLAEYQADQLAALGE
ECCCCCCCCEEECCCCCCCCCEEEEEEEEEHHHHHHCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA