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The map label for this gene is tolC [H]

Identifier: 66044190

GI number: 66044190

Start: 1069923

End: 1071332

Strand: Direct

Name: tolC [H]

Synonym: Psyr_0935

Alternate gene names: 66044190

Gene position: 1069923-1071332 (Clockwise)

Preceding gene: 66044189

Following gene: 66044192

Centisome position: 17.56

GC content: 60.0

Gene sequence:

>1410_bases
ATGACCGTGCTGCGCTTCGTTCCTGTGTTTCTTTCCCTCGGGTTGGCCAGCATTCAGGTCCATGCAGACCCCACGCAGCC
CGCTGCACCCCAGGTGACCAGCGCTTCGGCATACTCCGTGGATCTGATGAAGCTGTATCGCGAGGCTCGTCTGGAAGACC
CGCGCATGCTGTCTGCCTACCTGCGTGCACAGTCGGCAGTCGACAGCGAGCGCGATGCGTTCGGCGGGCTGTTGCCGCAA
GTAACTGCGAACGGCAGTTACAACCGGATCCTGCGCAAGGACGACACGCAACGCGAGATCTATAACAACTCCAGCTATTC
GCTCAACCTGACTCAGTACCTGTACAACAAGCAGGCTTGGGAAGCCTACCAGAAGGCCAAGAGCACGACGGAGCAGAAGG
GGCATCAGGCCGAAGATGCCCAGGCTGAGGCCACGGTTGATCTGGCCAAACGGTACTTCCAGGCGCTGTCTGCCGACGAT
GAACTCGAGCTGATCATGGCCGAGCGCCGGGCGACGCAAAAGAGCCTGGATCGGGTCAGTGCGATGTACGACAAAAAGAT
GGCCGTGGTCACTGATCTGCTGGATCTGAAAGCGCGTGTCGATTTGCTGGCCGCGCAGGAGGTTGATGCCCGTAACCAGA
TCAGGCTCAGCCGTGCTGCACTCTCTGAAATTGTCGGTCGGCCGATCACCGAGCCTTTGAGCCGCATCAGAAACGACATT
GCGCTGCAGGCGCCGTCCAAATCAATGGACACCTGGGTGGCCCAGGCCATCGATAGCAACCCGTTGCTCAAGGCGCGTGA
AAGCAGCCTCGACGTTGCCAACGCAGCGGTACGTGAAGGCAAGGGCGGGCATTATCCGACCCTGACCTTCACCCTGGGTG
CCCAGCAAAGTGACGTGGGCTACGACAACACCCTGTCACCGCGCAGCGACAGCTATGTGGCGACAATCGGGGTGCGCGTG
CCGATTTACAGCGGTGGCTCGACCTCTGCGCGAGTGCGTGGGCTGTACAACGAGCAACTGGCTACCGAACAGGACGTGGA
AAGCGTGCGCCGTCAGGTGGTCAAGGAAACCACCAACGCCTACCTGACGGCGTTTTCCAGTGTCGAGAAGATCCGCGCCA
ACAAGAACGCGCTGGCATCGGCAGAGCAATCGAGTATTGCGGCGCAGAAAGCCTTCTCCTATGGCGTTGTGAACGCGGTC
GATGTGCTGACCAGCGTGCAGAACGAGTTCAAGGCGCGACGTGACCTGCTCAGGGCGCAGTACGACTTCATCACCAACCT
GTTCATCCTCAATCGATGGGCAGGCCGTCCGCCACAGGAAAGTGTCGACAGCGTCAATGTCTGGCTGGGTGGTACCAATC
CGGCAGTCGAGAAGGCTGCGGACTCGGCCTTGAAAAAGACGCCCCGATAG

Upstream 100 bases:

>100_bases
GCATGCCCGCTGAAGTGCTGATCAATGCTGGTGAACGGACCATGCTTCAATACCTGTTGAAGCCGGCGTCCAATGTATTT
ATCAAATCGATGATCGAGGA

Downstream 100 bases:

>100_bases
CACCTGCGGCGCGCTTCAGGCTTGTGCCCGGCGTCTCTTCTAGCGATGAGTCGCCAGCACCTTGCGGTAGATATCCAGCG
TGCTCTGCGCACAGGCATCC

Product: Type I secretion outer membrane protein, TolC

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 469; Mature: 468

Protein sequence:

>469_residues
MTVLRFVPVFLSLGLASIQVHADPTQPAAPQVTSASAYSVDLMKLYREARLEDPRMLSAYLRAQSAVDSERDAFGGLLPQ
VTANGSYNRILRKDDTQREIYNNSSYSLNLTQYLYNKQAWEAYQKAKSTTEQKGHQAEDAQAEATVDLAKRYFQALSADD
ELELIMAERRATQKSLDRVSAMYDKKMAVVTDLLDLKARVDLLAAQEVDARNQIRLSRAALSEIVGRPITEPLSRIRNDI
ALQAPSKSMDTWVAQAIDSNPLLKARESSLDVANAAVREGKGGHYPTLTFTLGAQQSDVGYDNTLSPRSDSYVATIGVRV
PIYSGGSTSARVRGLYNEQLATEQDVESVRRQVVKETTNAYLTAFSSVEKIRANKNALASAEQSSIAAQKAFSYGVVNAV
DVLTSVQNEFKARRDLLRAQYDFITNLFILNRWAGRPPQESVDSVNVWLGGTNPAVEKAADSALKKTPR

Sequences:

>Translated_469_residues
MTVLRFVPVFLSLGLASIQVHADPTQPAAPQVTSASAYSVDLMKLYREARLEDPRMLSAYLRAQSAVDSERDAFGGLLPQ
VTANGSYNRILRKDDTQREIYNNSSYSLNLTQYLYNKQAWEAYQKAKSTTEQKGHQAEDAQAEATVDLAKRYFQALSADD
ELELIMAERRATQKSLDRVSAMYDKKMAVVTDLLDLKARVDLLAAQEVDARNQIRLSRAALSEIVGRPITEPLSRIRNDI
ALQAPSKSMDTWVAQAIDSNPLLKARESSLDVANAAVREGKGGHYPTLTFTLGAQQSDVGYDNTLSPRSDSYVATIGVRV
PIYSGGSTSARVRGLYNEQLATEQDVESVRRQVVKETTNAYLTAFSSVEKIRANKNALASAEQSSIAAQKAFSYGVVNAV
DVLTSVQNEFKARRDLLRAQYDFITNLFILNRWAGRPPQESVDSVNVWLGGTNPAVEKAADSALKKTPR
>Mature_468_residues
TVLRFVPVFLSLGLASIQVHADPTQPAAPQVTSASAYSVDLMKLYREARLEDPRMLSAYLRAQSAVDSERDAFGGLLPQV
TANGSYNRILRKDDTQREIYNNSSYSLNLTQYLYNKQAWEAYQKAKSTTEQKGHQAEDAQAEATVDLAKRYFQALSADDE
LELIMAERRATQKSLDRVSAMYDKKMAVVTDLLDLKARVDLLAAQEVDARNQIRLSRAALSEIVGRPITEPLSRIRNDIA
LQAPSKSMDTWVAQAIDSNPLLKARESSLDVANAAVREGKGGHYPTLTFTLGAQQSDVGYDNTLSPRSDSYVATIGVRVP
IYSGGSTSARVRGLYNEQLATEQDVESVRRQVVKETTNAYLTAFSSVEKIRANKNALASAEQSSIAAQKAFSYGVVNAVD
VLTSVQNEFKARRDLLRAQYDFITNLFILNRWAGRPPQESVDSVNVWLGGTNPAVEKAADSALKKTPR

Specific function: May be specialized for signal sequence independent, extracellular secretion in Gram-negative bacteria [H]

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Peripheral membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI87082199, Length=434, Percent_Identity=26.9585253456221, Blast_Score=126, Evalue=2e-30,

Paralogues:

None

Copy number: 120 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 240 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010130 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51758; Mature: 51626

Theoretical pI: Translated: 8.88; Mature: 8.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVLRFVPVFLSLGLASIQVHADPTQPAAPQVTSASAYSVDLMKLYREARLEDPRMLSAY
CCHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LRAQSAVDSERDAFGGLLPQVTANGSYNRILRKDDTQREIYNNSSYSLNLTQYLYNKQAW
HHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCCCEEEHHHHHHHHHHH
EAYQKAKSTTEQKGHQAEDAQAEATVDLAKRYFQALSADDELELIMAERRATQKSLDRVS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AMYDKKMAVVTDLLDLKARVDLLAAQEVDARNQIRLSRAALSEIVGRPITEPLSRIRNDI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALQAPSKSMDTWVAQAIDSNPLLKARESSLDVANAAVREGKGGHYPTLTFTLGAQQSDVG
EECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDNTLSPRSDSYVATIGVRVPIYSGGSTSARVRGLYNEQLATEQDVESVRRQVVKETTNA
CCCCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YLTAFSSVEKIRANKNALASAEQSSIAAQKAFSYGVVNAVDVLTSVQNEFKARRDLLRAQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDFITNLFILNRWAGRPPQESVDSVNVWLGGTNPAVEKAADSALKKTPR
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
TVLRFVPVFLSLGLASIQVHADPTQPAAPQVTSASAYSVDLMKLYREARLEDPRMLSAY
CHHHHHHHHHHCCHHEEEEECCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHH
LRAQSAVDSERDAFGGLLPQVTANGSYNRILRKDDTQREIYNNSSYSLNLTQYLYNKQAW
HHHHHHCCCHHHHHCCCCCCCCCCCCHHHHHCCCCCHHHHHCCCCCEEEHHHHHHHHHHH
EAYQKAKSTTEQKGHQAEDAQAEATVDLAKRYFQALSADDELELIMAERRATQKSLDRVS
HHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHH
AMYDKKMAVVTDLLDLKARVDLLAAQEVDARNQIRLSRAALSEIVGRPITEPLSRIRNDI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHH
ALQAPSKSMDTWVAQAIDSNPLLKARESSLDVANAAVREGKGGHYPTLTFTLGAQQSDVG
EECCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCCCCCCC
YDNTLSPRSDSYVATIGVRVPIYSGGSTSARVRGLYNEQLATEQDVESVRRQVVKETTNA
CCCCCCCCCCCEEEEEEEEEEEECCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
YLTAFSSVEKIRANKNALASAEQSSIAAQKAFSYGVVNAVDVLTSVQNEFKARRDLLRAQ
HHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YDFITNLFILNRWAGRPPQESVDSVNVWLGGTNPAVEKAADSALKKTPR
HHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]