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The map label for this gene is rfbA [H]

Identifier: 66044192

GI number: 66044192

Start: 1072797

End: 1074215

Strand: Direct

Name: rfbA [H]

Synonym: Psyr_0937

Alternate gene names: 66044192

Gene position: 1072797-1074215 (Clockwise)

Preceding gene: 66044190

Following gene: 66044193

Centisome position: 17.61

GC content: 59.2

Gene sequence:

>1419_bases
ATGTTCACCCCTGTAATCCTGGCTGGCGGTAGTGGTTCGCGGTTATGGCCTTTGTCGCGGCAGAGTTTCCCGAAGCAGTT
TCTTGCCCTTGATGGCCAGGATCAGGGAACCATGTTTCAACGCACACTGGCTCGCCTGAAAGGCCTTGAACACGCGCCTG
CGGTTGTTGTGAGCAACGAAAATCACCGCTTCATCGTCGCCGAGCAGCTACGTGTTGCGAAGATGGGCAGTCGTCGTGTC
ATCCTCGAGCCGCTCGCCCGCAACACTGCGCCGGCCATCGCCTTGGCTGCGCTCGAAGCAACGGCTGATGGCACTGACCC
GATCCTGCTGGTACTGGCTGCCGATCACCATATCCACGACGAAGAAGCTTTCCGACAAGCGGTAGCGGTTGCTCAGGTGC
ACGCCGAGGCGGGTCGCCTGGTGACGTTCGGGATTACCCCGACTCACGCTGAAACCGGTTTTGGCTACATCCACTGTGGT
GAATCCATTGCTCAGGGTGGCTTCGCAATCGAGGCGTTCAAGGAGAAGCCTTCGCCGGAAATGGCTGCCGAATACCTGTC
CTCGGGTGCTTACCTGTGGAACAGCGGTATGTTCATGTTCCGTGCCTCGGTGTTCCTGGCTGAGCTGAAAAAGCATCGTT
CCGACATATTGTCTGCATGCCGCGTAGCCTTGGCTGACTCCGATGCCGACAGCTACTTCCTGCACGTCTCGGCCGAGAAG
TTTGCCTTGTGCGCCGATGAGTCCGTGGACTACGCGGTCATGGAGCACACCGATCTCGGTCTGGTCGTACCTTTGGACGC
AGGCTGGAACGATCTGGGCAGTTGGGCAGCGATCTGGGACGTGGGCCCGCACGACGAGAATGTCAATCGCCTGGAAGGCG
ACGTGATGGCCATCGACACGCGCAACTGCCTGGTGCAGTCGCACCATCGACTGGTGGCCACCGTCGGCGTCGAGGACCTG
ATCGTCATCGAAACCAAGGACGCCGTTCTGGTTGCCAACAAGCACAACAGCCAGCAGGTCAAGGACGTGGTCAAGCGTCT
GCAGGCAGAAGAGCGCCCTGAGTTCGTTACTCATCCACTGGTCAACCGCCCGTGGGGCCACTACGACACGATCGATCTCG
GCGAGCGTTATCAGGTCAAGCGCATCAGCGTACTGCCAGGCGAGTGCCTGTCGCTGCAGATGCACTATCACCGCGCCGAG
CACTGGATCGTTGTATCCGGTACTGCCAAGGTTATCTGCGACGACAAGGAACTGATCCTTTCCGAGAATCAGTCCACTTA
CATCCCGCTGGGCGTCAAGCACTCGCTGGCCAACCCCGGCAAGGTGCCGCTGGAGCTGATCGAAGTGCAGTCCGGCTCGT
ACCTGGGCGAAGACGACATCGTGCGCTTTGAAGACCGTTACGGTCGTTTGAAGAAGTAA

Upstream 100 bases:

>100_bases
AATGGTAGGCATATTGTAGTGGCCTTCATGTTGCATAGCAGCCAGAGCACCCGGATAATGGGCCTTTGCCACGCCTTCGT
GGCCCAAGAATGGAGCGTCT

Downstream 100 bases:

>100_bases
GCTTCTCAGGCAGCGCTACACGTCTACCCAAACGCCCCGCAAGCGAGCTTGCTGGGCGTTTTTTGTTGGGCATGGTAATT
TTTCTACGGGTAGTTGTGGC

Product: mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase

Products: NA

Alternate protein names: GDP-mannose pyrophosphorylase; GMP; GMPP [H]

Number of amino acids: Translated: 472; Mature: 472

Protein sequence:

>472_residues
MFTPVILAGGSGSRLWPLSRQSFPKQFLALDGQDQGTMFQRTLARLKGLEHAPAVVVSNENHRFIVAEQLRVAKMGSRRV
ILEPLARNTAPAIALAALEATADGTDPILLVLAADHHIHDEEAFRQAVAVAQVHAEAGRLVTFGITPTHAETGFGYIHCG
ESIAQGGFAIEAFKEKPSPEMAAEYLSSGAYLWNSGMFMFRASVFLAELKKHRSDILSACRVALADSDADSYFLHVSAEK
FALCADESVDYAVMEHTDLGLVVPLDAGWNDLGSWAAIWDVGPHDENVNRLEGDVMAIDTRNCLVQSHHRLVATVGVEDL
IVIETKDAVLVANKHNSQQVKDVVKRLQAEERPEFVTHPLVNRPWGHYDTIDLGERYQVKRISVLPGECLSLQMHYHRAE
HWIVVSGTAKVICDDKELILSENQSTYIPLGVKHSLANPGKVPLELIEVQSGSYLGEDDIVRFEDRYGRLKK

Sequences:

>Translated_472_residues
MFTPVILAGGSGSRLWPLSRQSFPKQFLALDGQDQGTMFQRTLARLKGLEHAPAVVVSNENHRFIVAEQLRVAKMGSRRV
ILEPLARNTAPAIALAALEATADGTDPILLVLAADHHIHDEEAFRQAVAVAQVHAEAGRLVTFGITPTHAETGFGYIHCG
ESIAQGGFAIEAFKEKPSPEMAAEYLSSGAYLWNSGMFMFRASVFLAELKKHRSDILSACRVALADSDADSYFLHVSAEK
FALCADESVDYAVMEHTDLGLVVPLDAGWNDLGSWAAIWDVGPHDENVNRLEGDVMAIDTRNCLVQSHHRLVATVGVEDL
IVIETKDAVLVANKHNSQQVKDVVKRLQAEERPEFVTHPLVNRPWGHYDTIDLGERYQVKRISVLPGECLSLQMHYHRAE
HWIVVSGTAKVICDDKELILSENQSTYIPLGVKHSLANPGKVPLELIEVQSGSYLGEDDIVRFEDRYGRLKK
>Mature_472_residues
MFTPVILAGGSGSRLWPLSRQSFPKQFLALDGQDQGTMFQRTLARLKGLEHAPAVVVSNENHRFIVAEQLRVAKMGSRRV
ILEPLARNTAPAIALAALEATADGTDPILLVLAADHHIHDEEAFRQAVAVAQVHAEAGRLVTFGITPTHAETGFGYIHCG
ESIAQGGFAIEAFKEKPSPEMAAEYLSSGAYLWNSGMFMFRASVFLAELKKHRSDILSACRVALADSDADSYFLHVSAEK
FALCADESVDYAVMEHTDLGLVVPLDAGWNDLGSWAAIWDVGPHDENVNRLEGDVMAIDTRNCLVQSHHRLVATVGVEDL
IVIETKDAVLVANKHNSQQVKDVVKRLQAEERPEFVTHPLVNRPWGHYDTIDLGERYQVKRISVLPGECLSLQMHYHRAE
HWIVVSGTAKVICDDKELILSENQSTYIPLGVKHSLANPGKVPLELIEVQSGSYLGEDDIVRFEDRYGRLKK

Specific function: Involved In The Biosynthesis Of The Capsular Polysaccharide Colanic Acid. [C]

COG id: COG0836

COG function: function code M; Mannose-1-phosphate guanylyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mannose-6-phosphate isomerase type 2 family [H]

Homologues:

Organism=Escherichia coli, GI1788362, Length=473, Percent_Identity=57.0824524312896, Blast_Score=512, Evalue=1e-146,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011051
- InterPro:   IPR006375
- InterPro:   IPR001538
- InterPro:   IPR005835
- InterPro:   IPR014710 [H]

Pfam domain/function: PF01050 MannoseP_isomer; PF00483 NTP_transferase [H]

EC number: =2.7.7.13 [H]

Molecular weight: Translated: 52122; Mature: 52122

Theoretical pI: Translated: 5.86; Mature: 5.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFTPVILAGGSGSRLWPLSRQSFPKQFLALDGQDQGTMFQRTLARLKGLEHAPAVVVSNE
CCCCEEEECCCCCEEECCCHHHCCHHHEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECC
NHRFIVAEQLRVAKMGSRRVILEPLARNTAPAIALAALEATADGTDPILLVLAADHHIHD
CCEEEEHHHHHHHHCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCH
EEAFRQAVAVAQVHAEAGRLVTFGITPTHAETGFGYIHCGESIAQGGFAIEAFKEKPSPE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHCCCEEEEECCCCCCHH
MAAEYLSSGAYLWNSGMFMFRASVFLAELKKHRSDILSACRVALADSDADSYFLHVSAEK
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
FALCADESVDYAVMEHTDLGLVVPLDAGWNDLGSWAAIWDVGPHDENVNRLEGDVMAIDT
EEEECCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHCCCCEEEEEC
RNCLVQSHHRLVATVGVEDLIVIETKDAVLVANKHNSQQVKDVVKRLQAEERPEFVTHPL
HHHHHHCCCEEEEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCC
VNRPWGHYDTIDLGERYQVKRISVLPGECLSLQMHYHRAEHWIVVSGTAKVICDDKELIL
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCEEEEEECCEEEEECCCCEEE
SENQSTYIPLGVKHSLANPGKVPLELIEVQSGSYLGEDDIVRFEDRYGRLKK
ECCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEHHCCCCCC
>Mature Secondary Structure
MFTPVILAGGSGSRLWPLSRQSFPKQFLALDGQDQGTMFQRTLARLKGLEHAPAVVVSNE
CCCCEEEECCCCCEEECCCHHHCCHHHEEECCCCCCHHHHHHHHHHCCCCCCCEEEEECC
NHRFIVAEQLRVAKMGSRRVILEPLARNTAPAIALAALEATADGTDPILLVLAADHHIHD
CCEEEEHHHHHHHHCCCCEEEEEHHHCCCCCCEEEEEEECCCCCCCCEEEEEECCCCCCH
EEAFRQAVAVAQVHAEAGRLVTFGITPTHAETGFGYIHCGESIAQGGFAIEAFKEKPSPE
HHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCEEEEECHHHHCCCEEEEECCCCCCHH
MAAEYLSSGAYLWNSGMFMFRASVFLAELKKHRSDILSACRVALADSDADSYFLHVSAEK
HHHHHHHCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCC
FALCADESVDYAVMEHTDLGLVVPLDAGWNDLGSWAAIWDVGPHDENVNRLEGDVMAIDT
EEEECCCCCCEEEEEECCCCEEEECCCCCCCCCCEEEEEECCCCCCCCHHCCCCEEEEEC
RNCLVQSHHRLVATVGVEDLIVIETKDAVLVANKHNSQQVKDVVKRLQAEERPEFVTHPL
HHHHHHCCCEEEEEECCCEEEEEEECCEEEEECCCCHHHHHHHHHHHHHHCCCCHHCCCC
VNRPWGHYDTIDLGERYQVKRISVLPGECLSLQMHYHRAEHWIVVSGTAKVICDDKELIL
CCCCCCCCCCCCCCCCEEEEEEEECCCCCEEEEEEEECCCEEEEEECCEEEEECCCCEEE
SENQSTYIPLGVKHSLANPGKVPLELIEVQSGSYLGEDDIVRFEDRYGRLKK
ECCCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCCCEEEEHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1372980; 10952301 [H]