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The map label for this gene is rfbD [H]

Identifier: 66044170

GI number: 66044170

Start: 1039593

End: 1040489

Strand: Reverse

Name: rfbD [H]

Synonym: Psyr_0915

Alternate gene names: 66044170

Gene position: 1040489-1039593 (Counterclockwise)

Preceding gene: 66044171

Following gene: 66044169

Centisome position: 17.07

GC content: 61.32

Gene sequence:

>897_bases
ATGAGTATTGCTGGCAAGCGCGCACTGATCACCGGAATCAACGGCTTTACAGGCCGTTTCATGGCGAACGAGCTTGCCGC
CCAGGGCTGTGAAGTCCTTGGCGTCGGCAGTCAGCCTTCGGACAGTCCCGGCTATTACCAGGTGGATCTGGCAGACGTGG
CAGGTCTTCGCAAGCTGCTGGCAGACACTCAGCCGGATATCGTCGTGCACCTCGCAGCATTGGCGTTCGTCGGGCACGGG
GCAGCGGATGCTTTCTATCAGGTCAACCTGATCGGCACCCGTAACCTGCTCGAGGCGATCGATGCCTGCGGCAAGGTGCC
TGACTGTGTGTTGCTGGCAAGCAGCGCCAATGTCTACGGCAATGCTTCCTCCGGCATGCTGGATGAAACGACTCCGCCCG
CACCGGCCAACGACTATGCCGTGAGCAAACTGGCGATGGAATACATGGCGAGCCTCTGGCACGCCAGGTTGCCCATCGTC
ATCGCCAGGCCGTTCAACTACACCGGCGTCGGGCAGGCAGAAAACTTTCTGCTGCCCAAGATCGTTTCGCACTTCGCACG
CAAGGCCAGCACCATCGAGCTGGGCAACCTTGACGTGTGGCGTGATTTCAGTGACGTACGCGCCGTTGTCAGCGCCTATC
GCGGTCTGCTGGAAGTCTGCCCGGTCGGGCAGACGATCAACGTCAGCTCCGGCGTGACGCACTCGCTGCGCGAAGTGATC
GACATGTGCCGGGACATCACGGGCCAGGATATCGACGTTCAGGTGAACCCTGCGTTTGTCCGGGCCAATGAGGTCAAGAC
CTTGTGCGGCAACAACGCTCGCCTGCGCGCGTTGGTCCACGACTGGGAGACCCCGCCGCTTCGGGAAACCCTGAGCTGGA
TGTTGCCCTCCGCTTGA

Upstream 100 bases:

>100_bases
AGGAAGTCCTGGGCTGGGAGCCTAAGACCAATCTGGAAGAGCTGTGCCGCATGATGGTCGAAGCGGATCTGCGTCGTAAT
GAAAAAGGCTTTTCGTTCTG

Downstream 100 bases:

>100_bases
TTGGTTGGCAGTTACAAAATAAGGCCTGTCTACCTGGTAGACAGGCCTTTCCTTTTTAAACACCCTTAAAGCCCGTGGTG
AACGACTCAGCACGCATGGC

Product: NAD-dependent epimerase/dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase [H]

Number of amino acids: Translated: 298; Mature: 297

Protein sequence:

>298_residues
MSIAGKRALITGINGFTGRFMANELAAQGCEVLGVGSQPSDSPGYYQVDLADVAGLRKLLADTQPDIVVHLAALAFVGHG
AADAFYQVNLIGTRNLLEAIDACGKVPDCVLLASSANVYGNASSGMLDETTPPAPANDYAVSKLAMEYMASLWHARLPIV
IARPFNYTGVGQAENFLLPKIVSHFARKASTIELGNLDVWRDFSDVRAVVSAYRGLLEVCPVGQTINVSSGVTHSLREVI
DMCRDITGQDIDVQVNPAFVRANEVKTLCGNNARLRALVHDWETPPLRETLSWMLPSA

Sequences:

>Translated_298_residues
MSIAGKRALITGINGFTGRFMANELAAQGCEVLGVGSQPSDSPGYYQVDLADVAGLRKLLADTQPDIVVHLAALAFVGHG
AADAFYQVNLIGTRNLLEAIDACGKVPDCVLLASSANVYGNASSGMLDETTPPAPANDYAVSKLAMEYMASLWHARLPIV
IARPFNYTGVGQAENFLLPKIVSHFARKASTIELGNLDVWRDFSDVRAVVSAYRGLLEVCPVGQTINVSSGVTHSLREVI
DMCRDITGQDIDVQVNPAFVRANEVKTLCGNNARLRALVHDWETPPLRETLSWMLPSA
>Mature_297_residues
SIAGKRALITGINGFTGRFMANELAAQGCEVLGVGSQPSDSPGYYQVDLADVAGLRKLLADTQPDIVVHLAALAFVGHGA
ADAFYQVNLIGTRNLLEAIDACGKVPDCVLLASSANVYGNASSGMLDETTPPAPANDYAVSKLAMEYMASLWHARLPIVI
ARPFNYTGVGQAENFLLPKIVSHFARKASTIELGNLDVWRDFSDVRAVVSAYRGLLEVCPVGQTINVSSGVTHSLREVID
MCRDITGQDIDVQVNPAFVRANEVKTLCGNNARLRALVHDWETPPLRETLSWMLPSA

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG0451

COG function: function code MG; Nucleoside-diphosphate-sugar epimerases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI7657641, Length=271, Percent_Identity=27.3062730627306, Blast_Score=74, Evalue=2e-13,
Organism=Homo sapiens, GI4504031, Length=262, Percent_Identity=26.7175572519084, Blast_Score=72, Evalue=8e-13,
Organism=Escherichia coli, GI1788366, Length=347, Percent_Identity=26.2247838616715, Blast_Score=89, Evalue=4e-19,
Organism=Escherichia coli, GI48994969, Length=268, Percent_Identity=27.6119402985075, Blast_Score=70, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17507723, Length=327, Percent_Identity=28.1345565749235, Blast_Score=92, Evalue=4e-19,
Organism=Caenorhabditis elegans, GI133901786, Length=327, Percent_Identity=27.217125382263, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539422, Length=327, Percent_Identity=26.9113149847095, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17539424, Length=327, Percent_Identity=26.9113149847095, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI133901788, Length=327, Percent_Identity=26.9113149847095, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI133901790, Length=327, Percent_Identity=26.9113149847095, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17568069, Length=203, Percent_Identity=32.512315270936, Blast_Score=73, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI71982035, Length=299, Percent_Identity=27.7591973244147, Blast_Score=68, Evalue=7e-12,
Organism=Caenorhabditis elegans, GI71982038, Length=301, Percent_Identity=27.2425249169435, Blast_Score=66, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24158427, Length=316, Percent_Identity=25.9493670886076, Blast_Score=84, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 32000; Mature: 31869

Theoretical pI: Translated: 5.32; Mature: 5.32

Prosite motif: PS00226 IF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIAGKRALITGINGFTGRFMANELAAQGCEVLGVGSQPSDSPGYYQVDLADVAGLRKLL
CCCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHH
ADTQPDIVVHLAALAFVGHGAADAFYQVNLIGTRNLLEAIDACGKVPDCVLLASSANVYG
CCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCC
NASSGMLDETTPPAPANDYAVSKLAMEYMASLWHARLPIVIARPFNYTGVGQAENFLLPK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
IVSHFARKASTIELGNLDVWRDFSDVRAVVSAYRGLLEVCPVGQTINVSSGVTHSLREVI
HHHHHHHHCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
DMCRDITGQDIDVQVNPAFVRANEVKTLCGNNARLRALVHDWETPPLRETLSWMLPSA
HHHHCCCCCCEEEEECCCEEEHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCC
>Mature Secondary Structure 
SIAGKRALITGINGFTGRFMANELAAQGCEVLGVGSQPSDSPGYYQVDLADVAGLRKLL
CCCCCEEEEECCCCCHHHHHHHHHHHCCCEEEECCCCCCCCCCEEEEEHHHHHHHHHHH
ADTQPDIVVHLAALAFVGHGAADAFYQVNLIGTRNLLEAIDACGKVPDCVLLASSANVYG
CCCCCHHHHHHHHHHHHCCCCCCCEEEEEEECHHHHHHHHHHHCCCCCEEEEECCCCCCC
NASSGMLDETTPPAPANDYAVSKLAMEYMASLWHARLPIVIARPFNYTGVGQAENFLLPK
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCCCHHHH
IVSHFARKASTIELGNLDVWRDFSDVRAVVSAYRGLLEVCPVGQTINVSSGVTHSLREVI
HHHHHHHHCCEEEECCCHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCCHHHHHHHH
DMCRDITGQDIDVQVNPAFVRANEVKTLCGNNARLRALVHDWETPPLRETLSWMLPSA
HHHHCCCCCCEEEEECCCEEEHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1372980; 7540582; 10952301 [H]