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The map label for this gene is gmd [H]

Identifier: 66044171

GI number: 66044171

Start: 1040489

End: 1041520

Strand: Reverse

Name: gmd [H]

Synonym: Psyr_0916

Alternate gene names: 66044171

Gene position: 1041520-1040489 (Counterclockwise)

Preceding gene: 66044179

Following gene: 66044170

Centisome position: 17.09

GC content: 56.2

Gene sequence:

>1032_bases
ATGAAAGCAATCATTACCGGCATTACTGGTCAAGACGGCGCTTACCTGGCTGAGCTGCTGCTCGAAAAAGGCTATACCGT
TTACGGCACCTATCGCCGTACCAGCTCCGTCAATTTCTGGCGTATAGAAGAGCTCGGGATCCAGAACAACCCTAATCTGC
ATCTCGTCGAGTATGACCTGACTGACCTGTCTGCCAGCATTCGCTTGCTGCAAACTACTGGCGCTACCGAGGTCTACAAC
CTCGCGGCACAAAGCTTCGTGGGCGTGTCGTTCGAACAGCCGTTGACCACTGCCGAAATCACCGGCATCGGCGCAGTCAA
CCTGCTGGAAGCCATTCGCATCGTCAACACCAAGATCCGTTTCTACCAGGCATCGACTTCCGAGATGTTCGGCAAGGTCC
AGGCCATCCCGCAAGTAGAAAGCACTCCGTTCTACCCGCGCAGCCCGTATGGCGTCGCCAAGCTCTACGCGCACTGGATG
ACCATCAACTACCGTGAGTCGTACGACATCTTCGCCACCAGCGGCATCCTGTTCAACCACGAGTCGCCACTGCGTGGCCG
TGAGTTCGTGACCCGCAAGATCACCGATTCGGTTGCCAAGATCAAACTGGGCCTGATCGAGTCGTTCGAACTGGGCAACA
TGGACGCCAAGCGCGACTGGGGTTTTGCCAAGGAATACGTCGAAGGTATGTGGCGCATGCTGCAGGCTGATGAGCCGGAC
ACCTTTGTCCTGGCCACCAACCGCACCGAAACCGTACGCGACTTCGTGAGCATGGCGTTCAAGGCCACTGGCGTGACCAT
CAAGTGGGAAGGCGAAGCCGAGTCGGAAAAAGGCATTTGCGCAGACACTGGCAAGGTACTGGTTTCGGTCAATCCGAAGT
TCTACCGCCCAACCGAAGTCGAGCTGCTGATCGGTAACCCTGCAAAAGCCAAGGAAGTCCTGGGCTGGGAGCCTAAGACC
AATCTGGAAGAGCTGTGCCGCATGATGGTCGAAGCGGATCTGCGTCGTAATGAAAAAGGCTTTTCGTTCTGA

Upstream 100 bases:

>100_bases
ATATAGGGTTTCTAACCTGTATCATTGCACACCAGACATACAGTACAAGCCTCGAGAATAATCCGGGGCCCCGTTCTTCG
ATGGAGTTAAAAGACATAGC

Downstream 100 bases:

>100_bases
TGAGTATTGCTGGCAAGCGCGCACTGATCACCGGAATCAACGGCTTTACAGGCCGTTTCATGGCGAACGAGCTTGCCGCC
CAGGGCTGTGAAGTCCTTGG

Product: GDP-mannose 4,6-dehydratase

Products: NA

Alternate protein names: GDP-D-mannose dehydratase; ORF13.7 [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MKAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIEELGIQNNPNLHLVEYDLTDLSASIRLLQTTGATEVYN
LAAQSFVGVSFEQPLTTAEITGIGAVNLLEAIRIVNTKIRFYQASTSEMFGKVQAIPQVESTPFYPRSPYGVAKLYAHWM
TINYRESYDIFATSGILFNHESPLRGREFVTRKITDSVAKIKLGLIESFELGNMDAKRDWGFAKEYVEGMWRMLQADEPD
TFVLATNRTETVRDFVSMAFKATGVTIKWEGEAESEKGICADTGKVLVSVNPKFYRPTEVELLIGNPAKAKEVLGWEPKT
NLEELCRMMVEADLRRNEKGFSF

Sequences:

>Translated_343_residues
MKAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIEELGIQNNPNLHLVEYDLTDLSASIRLLQTTGATEVYN
LAAQSFVGVSFEQPLTTAEITGIGAVNLLEAIRIVNTKIRFYQASTSEMFGKVQAIPQVESTPFYPRSPYGVAKLYAHWM
TINYRESYDIFATSGILFNHESPLRGREFVTRKITDSVAKIKLGLIESFELGNMDAKRDWGFAKEYVEGMWRMLQADEPD
TFVLATNRTETVRDFVSMAFKATGVTIKWEGEAESEKGICADTGKVLVSVNPKFYRPTEVELLIGNPAKAKEVLGWEPKT
NLEELCRMMVEADLRRNEKGFSF
>Mature_343_residues
MKAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIEELGIQNNPNLHLVEYDLTDLSASIRLLQTTGATEVYN
LAAQSFVGVSFEQPLTTAEITGIGAVNLLEAIRIVNTKIRFYQASTSEMFGKVQAIPQVESTPFYPRSPYGVAKLYAHWM
TINYRESYDIFATSGILFNHESPLRGREFVTRKITDSVAKIKLGLIESFELGNMDAKRDWGFAKEYVEGMWRMLQADEPD
TFVLATNRTETVRDFVSMAFKATGVTIKWEGEAESEKGICADTGKVLVSVNPKFYRPTEVELLIGNPAKAKEVLGWEPKT
NLEELCRMMVEADLRRNEKGFSF

Specific function: Biosynthesis of the slime polysaccharide colanic acid. First of the three steps in the biosynthesis of GDP-fucose from GDP-mannose. [C]

COG id: COG1089

COG function: function code M; GDP-D-mannose dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GDP-mannose 4,6-dehydratase family [H]

Homologues:

Organism=Homo sapiens, GI4504031, Length=341, Percent_Identity=56.3049853372434, Blast_Score=393, Evalue=1e-109,
Organism=Escherichia coli, GI1788366, Length=353, Percent_Identity=52.1246458923513, Blast_Score=364, Evalue=1e-102,
Organism=Caenorhabditis elegans, GI17539424, Length=341, Percent_Identity=57.4780058651026, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI133901788, Length=341, Percent_Identity=57.4780058651026, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI133901790, Length=341, Percent_Identity=57.4780058651026, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17507723, Length=341, Percent_Identity=58.0645161290323, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI17539422, Length=341, Percent_Identity=57.4780058651026, Blast_Score=389, Evalue=1e-108,
Organism=Caenorhabditis elegans, GI133901786, Length=341, Percent_Identity=57.4780058651026, Blast_Score=389, Evalue=1e-108,
Organism=Drosophila melanogaster, GI24158427, Length=346, Percent_Identity=52.0231213872832, Blast_Score=355, Evalue=4e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001509
- InterPro:   IPR006368
- InterPro:   IPR016040 [H]

Pfam domain/function: PF01370 Epimerase [H]

EC number: =4.2.1.47 [H]

Molecular weight: Translated: 38648; Mature: 38648

Theoretical pI: Translated: 5.17; Mature: 5.17

Prosite motif: PS00061 ADH_SHORT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIEELGIQNNPNLHLVEYDL
CCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEC
TDLSASIRLLQTTGATEVYNLAAQSFVGVSFEQPLTTAEITGIGAVNLLEAIRIVNTKIR
CCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEE
FYQASTSEMFGKVQAIPQVESTPFYPRSPYGVAKLYAHWMTINYRESYDIFATSGILFNH
EEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCEEEECCEEECC
ESPLRGREFVTRKITDSVAKIKLGLIESFELGNMDAKRDWGFAKEYVEGMWRMLQADEPD
CCCCCHHHHHHHHHHHHHHHHEEHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
TFVLATNRTETVRDFVSMAFKATGVTIKWEGEAESEKGICADTGKVLVSVNPKFYRPTEV
EEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCEEEEECCCEECCCEE
ELLIGNPAKAKEVLGWEPKTNLEELCRMMVEADLRRNEKGFSF
EEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MKAIITGITGQDGAYLAELLLEKGYTVYGTYRRTSSVNFWRIEELGIQNNPNLHLVEYDL
CCEEEEECCCCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCCCEEEEEEEC
TDLSASIRLLQTTGATEVYNLAAQSFVGVSFEQPLTTAEITGIGAVNLLEAIRIVNTKIR
CCCCCEEEEEEECCCHHHHHHHHHHHCCCCCCCCCCEEEECCCCHHHHHHHHHHHHCEEE
FYQASTSEMFGKVQAIPQVESTPFYPRSPYGVAKLYAHWMTINYRESYDIFATSGILFNH
EEECCHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHEEEEECCCCCEEEECCEEECC
ESPLRGREFVTRKITDSVAKIKLGLIESFELGNMDAKRDWGFAKEYVEGMWRMLQADEPD
CCCCCHHHHHHHHHHHHHHHHEEHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHCCCCC
TFVLATNRTETVRDFVSMAFKATGVTIKWEGEAESEKGICADTGKVLVSVNPKFYRPTEV
EEEEECCCHHHHHHHHHHHHHHCCEEEEECCCCCCCCCEEECCCCEEEEECCCEECCCEE
ELLIGNPAKAKEVLGWEPKTNLEELCRMMVEADLRRNEKGFSF
EEEECCCCHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8932701 [H]