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The map label for this gene is pys2 [H]

Identifier: 66044138

GI number: 66044138

Start: 1005013

End: 1007049

Strand: Reverse

Name: pys2 [H]

Synonym: Psyr_0883

Alternate gene names: 66044138

Gene position: 1007049-1005013 (Counterclockwise)

Preceding gene: 66044139

Following gene: 66044137

Centisome position: 16.53

GC content: 53.12

Gene sequence:

>2037_bases
ATGCCGCAAAACGTCATTACTCTTCCACCCACAATCGTAACACCTGGCCAGCCGTTGCCCAGGCCATTAGGCCCGCTGTC
AGGAGCCGGAGTAATCGCCAGACCGCTGCGATGGGACGGCACTATTCCTGCAAACCATGAGCTGGATGCATTCTTCAGTA
AAAGGGGTATAAGAAACGAGCAGTACACGATATCCATTATCGTGACGGCCTCTTCAACACAGCAAAACATCCATCAGTCT
TACATAGACTTTTTGCCCCTCCTTCCGGCTGACGTTGACGCGGAAATTTCTGCCGGCGTAGGAGATTACCCCTTCTCTGA
GTTAGAAAAGTCTACGATTGAAAAAAACGTGGTGGACAGCCTGATTTCACAGCGCGCTGCTGAGTTAGCCAAGAGTAACG
AAGGCGCTCATGCTTTCTTTGGCAGGCATGTATTGGCGGTAGATATTAAAAAAAACGCTGTGGATTTTTTAAATATCTTT
CAAACAAGAAGAGACCTGGGCTCTCCCCTTGACGTTTATAAAAGCTGGGAAGCCTCAGTTACAGCAGCCTACAGAGCAAA
AATTCTTGAAGAGAAAATCAGGATGCTCACAGAGAGGTCGGTATCACTGAGCCATACTATTGCTGCAGCGCAGGCCAGGG
AGGACGCACGTATCGCGGCAGAAGCCGAGTCGACGAGGCTTGCCGTTGAAGCTGCAGAGCACGCTCGACTTGCTGCCGAA
ACCGCTGAACAAGCCCGAGTTGCAGTAGAGGCTGAAGCCAAGCGAGTAGCGGATGAGCAGGCTCTTCTTGCTGCCGAAGC
AGAAGCTCGCCGAGTAGCTGCCGAAGCGGCTGAACAAGCTCGTATGGAGGCAGAGACGCAGGCCCAGCGTGACGTTGACG
AGCACGCGCGCGTGACGGCAGAAGCTCAAGCGCTAGAAGCGGGCAAGACGCTCAGGCTCCCGGAAGCGGGTACACCGCAG
TTGGGTGCAGTTGCAGGAGTCATTTCCGTTACAGCAGGATCCGGTCTCTTTCTGGACGCTACCATTCAAGCCGCTATCGA
AATATTAACGGCTCTCGCAGGCACGGCTGTCAGCTCTACCACAGCCGTAGGCATCGGCACGTTGCTGTACTCCCCTTCGC
TTGGCAATGGAGAGTTACCGGAAAGAATGCTGGACCTTCCGGCCAGGGTTCTGATGCCTGATCTGCCCGACGCCTTGAAT
GACGTGGCGGCAACGGGTGGAACGGTCGATATGCCCTATAGAATTTACGGCGATCAGTCGAAGTATTCAGTCGTGGCAAC
ACAAGCCGAAGGCGGATTTTCTCCCAAGGTGCCTGTGAGGGCTCTGATACTGGACCCAGTGGCCAACGCCTACACCTTTA
CCACTTCGGACACCCCGCCCATCACCTTGACGTTTCCAATCGCAGCACCTGGAAACAGCTCAACGACGACGGTGGCGCAA
CCAGTCGAAATTCCGGCGTACGCTGGAATTACACTGGAACCTATCGAGGTGAAAGCCGAGCCTTTACCCGCCACCGGCCA
GATGGACATTCGGGACGCCATTTACGTTTATCCTTTAAATTCGGGTTTGCCTCCGGTCTATGCCGTGTTCAACAGCCCCT
ATGAAGGGGCAACAACCAAAGGAGAGCATAGCGGTCGAATGTACGATCCTGAAAAAGCGGGCGGCCCCACCCAAAACCTT
GACTGGACAACCGCCTCAGTCACTCAGGACGGCATTGATCTCGTTAAGCTGCACACAGGCCGATTCGGCTCATCAGATGC
AAATACTATAATGATTGATCGTCTGGAAAAAATTTTGCGCGGCGAGTTAGCCGTGACGGATACTGATAAAAGATTTTACA
CCCACGAGCTCAGGGAATTAGAACGTTACAGGGCACTGGGGGTGGCTGATGGCGTGCAAGGTAACGTATGGAATAACGCT
CATACGGCAGCCCTTGAGGATTATAGAATTAACGAAAATCGTGATTTTCTTTACACCGAAGCGGCTCAATCGGCTGGTGA
CAAACAAGATCATGCTGACGCATTGAGAGGCCAATGA

Upstream 100 bases:

>100_bases
AAAACAACTACCTATACCTATGTACCGCACTTCGTGACTATTAATACTACCTAATATCAAGGAGATATTATTGGGCGATT
TAAACCTTAAGGATAAGCAC

Downstream 100 bases:

>100_bases
AATGACCCACATCAGAGACATTATCATTAACGAAACCGTGGCAGATGTATTGGCAGTCCTGGCTCTAACTACGTCCCAAC
CACGGATAGACAGACTGTAC

Product: S-type Pyocin

Products: NA

Alternate protein names: Killer protein [H]

Number of amino acids: Translated: 678; Mature: 677

Protein sequence:

>678_residues
MPQNVITLPPTIVTPGQPLPRPLGPLSGAGVIARPLRWDGTIPANHELDAFFSKRGIRNEQYTISIIVTASSTQQNIHQS
YIDFLPLLPADVDAEISAGVGDYPFSELEKSTIEKNVVDSLISQRAAELAKSNEGAHAFFGRHVLAVDIKKNAVDFLNIF
QTRRDLGSPLDVYKSWEASVTAAYRAKILEEKIRMLTERSVSLSHTIAAAQAREDARIAAEAESTRLAVEAAEHARLAAE
TAEQARVAVEAEAKRVADEQALLAAEAEARRVAAEAAEQARMEAETQAQRDVDEHARVTAEAQALEAGKTLRLPEAGTPQ
LGAVAGVISVTAGSGLFLDATIQAAIEILTALAGTAVSSTTAVGIGTLLYSPSLGNGELPERMLDLPARVLMPDLPDALN
DVAATGGTVDMPYRIYGDQSKYSVVATQAEGGFSPKVPVRALILDPVANAYTFTTSDTPPITLTFPIAAPGNSSTTTVAQ
PVEIPAYAGITLEPIEVKAEPLPATGQMDIRDAIYVYPLNSGLPPVYAVFNSPYEGATTKGEHSGRMYDPEKAGGPTQNL
DWTTASVTQDGIDLVKLHTGRFGSSDANTIMIDRLEKILRGELAVTDTDKRFYTHELRELERYRALGVADGVQGNVWNNA
HTAALEDYRINENRDFLYTEAAQSAGDKQDHADALRGQ

Sequences:

>Translated_678_residues
MPQNVITLPPTIVTPGQPLPRPLGPLSGAGVIARPLRWDGTIPANHELDAFFSKRGIRNEQYTISIIVTASSTQQNIHQS
YIDFLPLLPADVDAEISAGVGDYPFSELEKSTIEKNVVDSLISQRAAELAKSNEGAHAFFGRHVLAVDIKKNAVDFLNIF
QTRRDLGSPLDVYKSWEASVTAAYRAKILEEKIRMLTERSVSLSHTIAAAQAREDARIAAEAESTRLAVEAAEHARLAAE
TAEQARVAVEAEAKRVADEQALLAAEAEARRVAAEAAEQARMEAETQAQRDVDEHARVTAEAQALEAGKTLRLPEAGTPQ
LGAVAGVISVTAGSGLFLDATIQAAIEILTALAGTAVSSTTAVGIGTLLYSPSLGNGELPERMLDLPARVLMPDLPDALN
DVAATGGTVDMPYRIYGDQSKYSVVATQAEGGFSPKVPVRALILDPVANAYTFTTSDTPPITLTFPIAAPGNSSTTTVAQ
PVEIPAYAGITLEPIEVKAEPLPATGQMDIRDAIYVYPLNSGLPPVYAVFNSPYEGATTKGEHSGRMYDPEKAGGPTQNL
DWTTASVTQDGIDLVKLHTGRFGSSDANTIMIDRLEKILRGELAVTDTDKRFYTHELRELERYRALGVADGVQGNVWNNA
HTAALEDYRINENRDFLYTEAAQSAGDKQDHADALRGQ
>Mature_677_residues
PQNVITLPPTIVTPGQPLPRPLGPLSGAGVIARPLRWDGTIPANHELDAFFSKRGIRNEQYTISIIVTASSTQQNIHQSY
IDFLPLLPADVDAEISAGVGDYPFSELEKSTIEKNVVDSLISQRAAELAKSNEGAHAFFGRHVLAVDIKKNAVDFLNIFQ
TRRDLGSPLDVYKSWEASVTAAYRAKILEEKIRMLTERSVSLSHTIAAAQAREDARIAAEAESTRLAVEAAEHARLAAET
AEQARVAVEAEAKRVADEQALLAAEAEARRVAAEAAEQARMEAETQAQRDVDEHARVTAEAQALEAGKTLRLPEAGTPQL
GAVAGVISVTAGSGLFLDATIQAAIEILTALAGTAVSSTTAVGIGTLLYSPSLGNGELPERMLDLPARVLMPDLPDALND
VAATGGTVDMPYRIYGDQSKYSVVATQAEGGFSPKVPVRALILDPVANAYTFTTSDTPPITLTFPIAAPGNSSTTTVAQP
VEIPAYAGITLEPIEVKAEPLPATGQMDIRDAIYVYPLNSGLPPVYAVFNSPYEGATTKGEHSGRMYDPEKAGGPTQNLD
WTTASVTQDGIDLVKLHTGRFGSSDANTIMIDRLEKILRGELAVTDTDKRFYTHELRELERYRALGVADGVQGNVWNNAH
TAALEDYRINENRDFLYTEAAQSAGDKQDHADALRGQ

Specific function: Causes breakdown of chromosomal DNA as well as complete inhibition of lipid synthesis in sensitive cells [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the colicin/pyosin nuclease family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003615
- InterPro:   IPR003060
- InterPro:   IPR010715
- InterPro:   IPR016128 [H]

Pfam domain/function: PF06958 Pyocin_S [H]

EC number: NA

Molecular weight: Translated: 72726; Mature: 72595

Theoretical pI: Translated: 4.60; Mature: 4.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPQNVITLPPTIVTPGQPLPRPLGPLSGAGVIARPLRWDGTIPANHELDAFFSKRGIRNE
CCCCEEECCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCC
QYTISIIVTASSTQQNIHQSYIDFLPLLPADVDAEISAGVGDYPFSELEKSTIEKNVVDS
CEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
LISQRAAELAKSNEGAHAFFGRHVLAVDIKKNAVDFLNIFQTRRDLGSPLDVYKSWEASV
HHHHHHHHHHHCCCCCEEECCCEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHCCCHHH
TAAYRAKILEEKIRMLTERSVSLSHTIAAAQAREDARIAAEAESTRLAVEAAEHARLAAE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCHHEEEHHHHHHHHHHHH
TAEQARVAVEAEAKRVADEQALLAAEAEARRVAAEAAEQARMEAETQAQRDVDEHARVTA
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
EAQALEAGKTLRLPEAGTPQLGAVAGVISVTAGSGLFLDATIQAAIEILTALAGTAVSST
HHHHHHCCCEEECCCCCCCCHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHCCHHCCH
TAVGIGTLLYSPSLGNGELPERMLDLPARVLMPDLPDALNDVAATGGTVDMPYRIYGDQS
HHHHHHHHEECCCCCCCCCCHHHHCCCHHHHCCCCCHHHHHHHHCCCCCCCCEEEECCCC
KYSVVATQAEGGFSPKVPVRALILDPVANAYTFTTSDTPPITLTFPIAAPGNSSTTTVAQ
CEEEEEEECCCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEEECCCCCCCCEEEC
PVEIPAYAGITLEPIEVKAEPLPATGQMDIRDAIYVYPLNSGLPPVYAVFNSPYEGATTK
CCCCCCCCCCEEECEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCC
GEHSGRMYDPEKAGGPTQNLDWTTASVTQDGIDLVKLHTGRFGSSDANTIMIDRLEKILR
CCCCCCCCCCHHCCCCCCCCCCEEHHCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHC
GELAVTDTDKRFYTHELRELERYRALGVADGVQGNVWNNAHTAALEDYRINENRDFLYTE
CCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECEEECCCCCEEEEH
AAQSAGDKQDHADALRGQ
HHHHCCCCHHHHHHHCCC
>Mature Secondary Structure 
PQNVITLPPTIVTPGQPLPRPLGPLSGAGVIARPLRWDGTIPANHELDAFFSKRGIRNE
CCCEEECCCEEECCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCCHHHHHHHCCCCCC
QYTISIIVTASSTQQNIHQSYIDFLPLLPADVDAEISAGVGDYPFSELEKSTIEKNVVDS
CEEEEEEEEECCHHHHHHHHHHHHHHCCCCCCCCHHCCCCCCCCHHHHHHHHHHHHHHHH
LISQRAAELAKSNEGAHAFFGRHVLAVDIKKNAVDFLNIFQTRRDLGSPLDVYKSWEASV
HHHHHHHHHHHCCCCCEEECCCEEEEEEECHHHHHHHHHHHHHHHCCCCHHHHHCCCHHH
TAAYRAKILEEKIRMLTERSVSLSHTIAAAQAREDARIAAEAESTRLAVEAAEHARLAAE
HHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHCCCCEEECCCHHEEEHHHHHHHHHHHH
TAEQARVAVEAEAKRVADEQALLAAEAEARRVAAEAAEQARMEAETQAQRDVDEHARVTA
HHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHH
EAQALEAGKTLRLPEAGTPQLGAVAGVISVTAGSGLFLDATIQAAIEILTALAGTAVSST
HHHHHHCCCEEECCCCCCCCHHHHHHEEEEECCCCEEEHHHHHHHHHHHHHHHCCHHCCH
TAVGIGTLLYSPSLGNGELPERMLDLPARVLMPDLPDALNDVAATGGTVDMPYRIYGDQS
HHHHHHHHEECCCCCCCCCCHHHHCCCHHHHCCCCCHHHHHHHHCCCCCCCCEEEECCCC
KYSVVATQAEGGFSPKVPVRALILDPVANAYTFTTSDTPPITLTFPIAAPGNSSTTTVAQ
CEEEEEEECCCCCCCCCCEEEEEECCCCCCEEEECCCCCCEEEEEEEECCCCCCCCEEEC
PVEIPAYAGITLEPIEVKAEPLPATGQMDIRDAIYVYPLNSGLPPVYAVFNSPYEGATTK
CCCCCCCCCCEEECEEEECCCCCCCCCCCCCCEEEEEECCCCCCCEEEEECCCCCCCCCC
GEHSGRMYDPEKAGGPTQNLDWTTASVTQDGIDLVKLHTGRFGSSDANTIMIDRLEKILR
CCCCCCCCCCHHCCCCCCCCCCEEHHCCCCCCEEEEEECCCCCCCCCCEEHHHHHHHHHC
GELAVTDTDKRFYTHELRELERYRALGVADGVQGNVWNNAHTAALEDYRINENRDFLYTE
CCEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCHHEEECEEECCCCCEEEEH
AAQSAGDKQDHADALRGQ
HHHHCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8491711; 10984043 [H]