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The map label for this gene is pcnB [H]

Identifier: 66044085

GI number: 66044085

Start: 939218

End: 940693

Strand: Reverse

Name: pcnB [H]

Synonym: Psyr_0830

Alternate gene names: 66044085

Gene position: 940693-939218 (Counterclockwise)

Preceding gene: 66044086

Following gene: 66044084

Centisome position: 15.44

GC content: 60.77

Gene sequence:

>1476_bases
ATGCTAGAATCCCCGCCCATCATGCGGTCATTCCACGTTTTTGGCCGAATATTCCTTCAAACAGTGCATCCCATGCTGAA
GAAGTTGTTCCAGTCATTTCGTTCCCCACTGCGCAAACCGCAGCAACATACGCGCACCACGCCTGAAGTGCTCAACAGCA
GCCAACATTCGCTGCAACGCAGTCAGTTCAGCCGCTATGCCGTGAATATCGTCGAGCGCCTGCAGAACGCCGGCTACCAG
GCCTATCTGGTCGGTGGCTGCGTACGCGACATGATGCTCAACATCACGCCCAAGGATTTCGACGTCGCCACCAGCGCCAC
GCCCGAACAGGTGCGTGCCGAATTCCGCAACGCGCGGATCATCGGCCGCCGGTTCAAGCTGGTGCACATCCACTTCGGTC
GCGAGATCATCGAAGTGGCGACGTTCCGCGCCAATCATCCCCAGGATGACGAAGAAGAGGACAGCAACCAGTCCTCGCGC
AATGAAAGCGGACGCATCCTGCGTGACAACGTATACGGCACGCTTGAAGAAGACGCCCAGCGTCGTGACTTCACGATCAA
CGCGCTTTACTACGACCCGGTCAGCGAACGCGTCCTGGATTACGCCAATGGCGTGCACGACATCCGCAATCGCCTGATCC
GCCTGATCGGCGATCCGGAGCAGCGTTATAAGGAAGACCCGGTGCGCATGCTGCGTGCCGTGCGTTTCGCTGCCAAGCTG
GACTTCGGTATCGAGAAGCACAGCGCTCAGCCGATCCGTGCACTGGCGCCGATGCTGCGTGACATTCCGTCGGCACGCCT
GTTCGAGGAAGTGCTCAAACTGTTCCTCTCCGGGCATGCCGCCCCCACCTTTGAAATGCTGGTGGACCTGGAACTGTTCG
AACCGTTGTTCCCGGCCAGCTCCAAGGCCCTGGAATACAATCCGACCTACACCCATACGCTGATCAGCAATGCGCTGATC
AATACCGACCTGCGCATCAAGCAGAACAAACCGGTCACGCCGGCGTTCCTGTTCGCTGCCCTGCTCTGGCCTGCGCTACC
GGCCAAGGTACTGCGTGCCCAGGAACGTGGCATGCCGCCGATCGCAGCCATGCAGGAAGCCGCTCACGAACTGATCATCG
AACAGTGCCAGCGCATCGCTATCCCCAAGCGCTTCACGCTGCCGATCCGCGAGATCTGGGACATGCAGGAGCGCCTGCCG
CGCCGTTCGGGCAAACGTGCCGACCTACTGCTTGATAACTCGCGGTTCCGCGCCGGCTACGACTTCCTGCTGTTGCGTGA
AACGGCGGGCGAGCAAACCGATGGCCTGGGTCAGTGGTGGACCGATTATCAGGACTGCAACGACAGCGAACGCCGCGACA
TGATTCGCGACCTGAGCAACAAGCCCGAGGCGGCCGGCACAGCCCCGCGCAAGCGGCGTCGTAACAGCGGCGCCAAACGC
AAGCGCACCACGGGCGAGGCGCAAAGCGGTGAATGA

Upstream 100 bases:

>100_bases
ACACTTTGGGGGAGCTTCGGCTCCCCCAGTAGCTTCCCCGCCTTGATCCGCGCCATCGTTTACCCTTCCGCGACAGCTTC
CTACACCATCCCTCGACTAA

Downstream 100 bases:

>100_bases
ACCGCTGTTGGCCACTGAACGCGTGTACATCGGTCTGGGCAGCAATCTGGCTGACCCTGCCGAGCAATTGCGCAGCGCCC
TCAAGGCCATTGCGCAGTTA

Product: Poly(A) polymerase

Products: NA

Alternate protein names: PAP [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MLESPPIMRSFHVFGRIFLQTVHPMLKKLFQSFRSPLRKPQQHTRTTPEVLNSSQHSLQRSQFSRYAVNIVERLQNAGYQ
AYLVGGCVRDMMLNITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRANHPQDDEEEDSNQSSR
NESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERVLDYANGVHDIRNRLIRLIGDPEQRYKEDPVRMLRAVRFAAKL
DFGIEKHSAQPIRALAPMLRDIPSARLFEEVLKLFLSGHAAPTFEMLVDLELFEPLFPASSKALEYNPTYTHTLISNALI
NTDLRIKQNKPVTPAFLFAALLWPALPAKVLRAQERGMPPIAAMQEAAHELIIEQCQRIAIPKRFTLPIREIWDMQERLP
RRSGKRADLLLDNSRFRAGYDFLLLRETAGEQTDGLGQWWTDYQDCNDSERRDMIRDLSNKPEAAGTAPRKRRRNSGAKR
KRTTGEAQSGE

Sequences:

>Translated_491_residues
MLESPPIMRSFHVFGRIFLQTVHPMLKKLFQSFRSPLRKPQQHTRTTPEVLNSSQHSLQRSQFSRYAVNIVERLQNAGYQ
AYLVGGCVRDMMLNITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRANHPQDDEEEDSNQSSR
NESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERVLDYANGVHDIRNRLIRLIGDPEQRYKEDPVRMLRAVRFAAKL
DFGIEKHSAQPIRALAPMLRDIPSARLFEEVLKLFLSGHAAPTFEMLVDLELFEPLFPASSKALEYNPTYTHTLISNALI
NTDLRIKQNKPVTPAFLFAALLWPALPAKVLRAQERGMPPIAAMQEAAHELIIEQCQRIAIPKRFTLPIREIWDMQERLP
RRSGKRADLLLDNSRFRAGYDFLLLRETAGEQTDGLGQWWTDYQDCNDSERRDMIRDLSNKPEAAGTAPRKRRRNSGAKR
KRTTGEAQSGE
>Mature_491_residues
MLESPPIMRSFHVFGRIFLQTVHPMLKKLFQSFRSPLRKPQQHTRTTPEVLNSSQHSLQRSQFSRYAVNIVERLQNAGYQ
AYLVGGCVRDMMLNITPKDFDVATSATPEQVRAEFRNARIIGRRFKLVHIHFGREIIEVATFRANHPQDDEEEDSNQSSR
NESGRILRDNVYGTLEEDAQRRDFTINALYYDPVSERVLDYANGVHDIRNRLIRLIGDPEQRYKEDPVRMLRAVRFAAKL
DFGIEKHSAQPIRALAPMLRDIPSARLFEEVLKLFLSGHAAPTFEMLVDLELFEPLFPASSKALEYNPTYTHTLISNALI
NTDLRIKQNKPVTPAFLFAALLWPALPAKVLRAQERGMPPIAAMQEAAHELIIEQCQRIAIPKRFTLPIREIWDMQERLP
RRSGKRADLLLDNSRFRAGYDFLLLRETAGEQTDGLGQWWTDYQDCNDSERRDMIRDLSNKPEAAGTAPRKRRRNSGAKR
KRTTGEAQSGE

Specific function: Polymerase that creates the 3' poly(A) tail found in some mRNA's [H]

COG id: COG0617

COG function: function code J; tRNA nucleotidyltransferase/poly(A) polymerase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA nucleotidyltransferase/poly(A) polymerase family [H]

Homologues:

Organism=Homo sapiens, GI155030240, Length=257, Percent_Identity=26.4591439688716, Blast_Score=100, Evalue=4e-21,
Organism=Escherichia coli, GI87081691, Length=422, Percent_Identity=47.39336492891, Blast_Score=382, Evalue=1e-107,
Organism=Escherichia coli, GI1789436, Length=318, Percent_Identity=29.2452830188679, Blast_Score=85, Evalue=1e-17,
Organism=Caenorhabditis elegans, GI71995920, Length=270, Percent_Identity=28.1481481481481, Blast_Score=91, Evalue=1e-18,
Organism=Drosophila melanogaster, GI281360112, Length=244, Percent_Identity=27.4590163934426, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI21357337, Length=244, Percent_Identity=27.4590163934426, Blast_Score=85, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002646
- InterPro:   IPR010206 [H]

Pfam domain/function: PF01743 PolyA_pol [H]

EC number: =2.7.7.19 [H]

Molecular weight: Translated: 56576; Mature: 56576

Theoretical pI: Translated: 9.83; Mature: 9.83

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLESPPIMRSFHVFGRIFLQTVHPMLKKLFQSFRSPLRKPQQHTRTTPEVLNSSQHSLQR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHCCHHHHHHH
SQFSRYAVNIVERLQNAGYQAYLVGGCVRDMMLNITPKDFDVATSATPEQVRAEFRNARI
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
IGRRFKLVHIHFGREIIEVATFRANHPQDDEEEDSNQSSRNESGRILRDNVYGTLEEDAQ
EECEEEEEEEEHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCHHHHHH
RRDFTINALYYDPVSERVLDYANGVHDIRNRLIRLIGDPEQRYKEDPVRMLRAVRFAAKL
HCCEEEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DFGIEKHSAQPIRALAPMLRDIPSARLFEEVLKLFLSGHAAPTFEMLVDLELFEPLFPAS
HCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
SKALEYNPTYTHTLISNALINTDLRIKQNKPVTPAFLFAALLWPALPAKVLRAQERGMPP
CCCEECCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
IAAMQEAAHELIIEQCQRIAIPKRFTLPIREIWDMQERLPRRSGKRADLLLDNSRFRAGY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
DFLLLRETAGEQTDGLGQWWTDYQDCNDSERRDMIRDLSNKPEAAGTAPRKRRRNSGAKR
CEEEEECCCCCCCCCCHHHHCCHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHH
KRTTGEAQSGE
HCCCCCCCCCC
>Mature Secondary Structure
MLESPPIMRSFHVFGRIFLQTVHPMLKKLFQSFRSPLRKPQQHTRTTPEVLNSSQHSLQR
CCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHCCCCHHHHCCHHHHHHH
SQFSRYAVNIVERLQNAGYQAYLVGGCVRDMMLNITPKDFDVATSATPEQVRAEFRNARI
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHH
IGRRFKLVHIHFGREIIEVATFRANHPQDDEEEDSNQSSRNESGRILRDNVYGTLEEDAQ
EECEEEEEEEEHHHHHHHHHHHCCCCCCCCCHHHCCCCCCCCCCCEEECCCCCCHHHHHH
RRDFTINALYYDPVSERVLDYANGVHDIRNRLIRLIGDPEQRYKEDPVRMLRAVRFAAKL
HCCEEEEEEEECCHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
DFGIEKHSAQPIRALAPMLRDIPSARLFEEVLKLFLSGHAAPTFEMLVDLELFEPLFPAS
HCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCCCC
SKALEYNPTYTHTLISNALINTDLRIKQNKPVTPAFLFAALLWPALPAKVLRAQERGMPP
CCCEECCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCH
IAAMQEAAHELIIEQCQRIAIPKRFTLPIREIWDMQERLPRRSGKRADLLLDNSRFRAGY
HHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCCCCCEEECCCCCCCCC
DFLLLRETAGEQTDGLGQWWTDYQDCNDSERRDMIRDLSNKPEAAGTAPRKRRRNSGAKR
CEEEEECCCCCCCCCCHHHHCCHHCCCCHHHHHHHHHHCCCCCCCCCCHHHHHHCCCCHH
KRTTGEAQSGE
HCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]