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The map label for this gene is rutR [H]

Identifier: 66043942

GI number: 66043942

Start: 765199

End: 765861

Strand: Reverse

Name: rutR [H]

Synonym: Psyr_0675

Alternate gene names: 66043942

Gene position: 765861-765199 (Counterclockwise)

Preceding gene: 66043943

Following gene: 66043941

Centisome position: 12.57

GC content: 58.97

Gene sequence:

>663_bases
ATGACCCTTGAAGTCCCCGCACACAGCGCTCACGTCACCAAGCCTGCAAGCCGTATTCGTCAGAAGAACGAAGAAGCGAT
CATCCAGGCAGCCGAAGATGAATTCGCCCGCCACGGTTTCAAGGGCACCAGCATGAACACCATCGCGATCAAGGCCGGGC
TGCCAAAGGCCAATCTGCATTACTACTTCACCAATAAACTCGGCCTGTATGTCGCGGTGCTCAGCAACATCATCGATTTG
TGGGACAGCACCTTCAACCACCTCAGCGTCGAAGACGATCCCGCAGAGGCGCTGGCCCGCTATATTCGCGCCAAGATGGA
GTTCTCGCGCCGCCAGCCACAGGCTTCGCGGATCTTCGCCATGGAGATCATCAGCGGCGGCGAATGCATGAGCGAGTATT
TCAGTCAGGATTACCGTCTGTGGTTTCAGGGCCGGGCCGCGGTCTTTCAAGCCTGGATCGACGCCGGCAAGATGGACCCC
GTCGACCCGGTGCACCTGATCTTCCTGCTATGGGGCAGCACCCAGCACTATGCCGACTTCGCCTCGCAGATCTGTCGCGT
CACCGGTCGCTCCCGACTGACGCGCCAGGACATGGATCAGGCCAGCAACAATCTCATTCGAATCATCCTCAAAGGTTGTG
GGCTCGCGCCGCCAGCCTTGTAG

Upstream 100 bases:

>100_bases
CGATAACTTTGCATGCCGCGCCCGCACCGACGCTTGCGAGATTCTCGCCGGTGCGCTATCTGTTCATCCGTCGCGCCCTA
CGACCTGTCCGGAATTGCCC

Downstream 100 bases:

>100_bases
AATCGTCGCCTTGACGTCAGCTGGACACACGCATGCCTTTTACCCTGGTCGGCCCTTGCGAGTTTCGTGAAGAAATCCGC
AAGAGCCGCTTCATCACCCT

Product: regulatory protein, TetR

Products: NA

Alternate protein names: Rut operon repressor [H]

Number of amino acids: Translated: 220; Mature: 219

Protein sequence:

>220_residues
MTLEVPAHSAHVTKPASRIRQKNEEAIIQAAEDEFARHGFKGTSMNTIAIKAGLPKANLHYYFTNKLGLYVAVLSNIIDL
WDSTFNHLSVEDDPAEALARYIRAKMEFSRRQPQASRIFAMEIISGGECMSEYFSQDYRLWFQGRAAVFQAWIDAGKMDP
VDPVHLIFLLWGSTQHYADFASQICRVTGRSRLTRQDMDQASNNLIRIILKGCGLAPPAL

Sequences:

>Translated_220_residues
MTLEVPAHSAHVTKPASRIRQKNEEAIIQAAEDEFARHGFKGTSMNTIAIKAGLPKANLHYYFTNKLGLYVAVLSNIIDL
WDSTFNHLSVEDDPAEALARYIRAKMEFSRRQPQASRIFAMEIISGGECMSEYFSQDYRLWFQGRAAVFQAWIDAGKMDP
VDPVHLIFLLWGSTQHYADFASQICRVTGRSRLTRQDMDQASNNLIRIILKGCGLAPPAL
>Mature_219_residues
TLEVPAHSAHVTKPASRIRQKNEEAIIQAAEDEFARHGFKGTSMNTIAIKAGLPKANLHYYFTNKLGLYVAVLSNIIDLW
DSTFNHLSVEDDPAEALARYIRAKMEFSRRQPQASRIFAMEIISGGECMSEYFSQDYRLWFQGRAAVFQAWIDAGKMDPV
DPVHLIFLLWGSTQHYADFASQICRVTGRSRLTRQDMDQASNNLIRIILKGCGLAPPAL

Specific function: Master transcription regulator which represses the degradation of pyrimidines (rutABCDEFG) and purines (gcl operon) for maintenance of metabolic balance between pyrimidines and purines. It also regulates the synthesis of pyrimidine nucleotides and arginin

COG id: COG1309

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH tetR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787249, Length=205, Percent_Identity=40, Blast_Score=168, Evalue=2e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR015893
- InterPro:   IPR011075
- InterPro:   IPR019915
- InterPro:   IPR001647
- InterPro:   IPR013573 [H]

Pfam domain/function: PF08362 TetR_C_3; PF00440 TetR_N [H]

EC number: NA

Molecular weight: Translated: 24808; Mature: 24676

Theoretical pI: Translated: 8.10; Mature: 8.10

Prosite motif: PS50977 HTH_TETR_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLEVPAHSAHVTKPASRIRQKNEEAIIQAAEDEFARHGFKGTSMNTIAIKAGLPKANLH
CEECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEE
YYFTNKLGLYVAVLSNIIDLWDSTFNHLSVEDDPAEALARYIRAKMEFSRRQPQASRIFA
EEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
MEIISGGECMSEYFSQDYRLWFQGRAAVFQAWIDAGKMDPVDPVHLIFLLWGSTQHYADF
HHHHCCHHHHHHHHCHHHEEEECHHHHHHHHHHHCCCCCCCCHHHEEHHHCCCCHHHHHH
ASQICRVTGRSRLTRQDMDQASNNLIRIILKGCGLAPPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
TLEVPAHSAHVTKPASRIRQKNEEAIIQAAEDEFARHGFKGTSMNTIAIKAGLPKANLH
EECCCCCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCCCCEE
YYFTNKLGLYVAVLSNIIDLWDSTFNHLSVEDDPAEALARYIRAKMEFSRRQPQASRIFA
EEEECHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHH
MEIISGGECMSEYFSQDYRLWFQGRAAVFQAWIDAGKMDPVDPVHLIFLLWGSTQHYADF
HHHHCCHHHHHHHHCHHHEEEECHHHHHHHHHHHCCCCCCCCHHHEEHHHCCCCHHHHHH
ASQICRVTGRSRLTRQDMDQASNNLIRIILKGCGLAPPAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]