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The map label for this gene is rho [H]

Identifier: 66043571

GI number: 66043571

Start: 320320

End: 321579

Strand: Direct

Name: rho [H]

Synonym: Psyr_0301

Alternate gene names: 66043571

Gene position: 320320-321579 (Clockwise)

Preceding gene: 66043570

Following gene: 66043572

Centisome position: 5.26

GC content: 57.38

Gene sequence:

>1260_bases
ATGAACCTGACTGAACTCAAGCAAAAGCCGATTACCGAATTGCTCGAAATGGCCGAACAGGCTGGCATCGAGAATATGGC
CCGTTCGCGCAAGCAGGACGTCATTTTCTCCCTGCTCAAACGGCACGCCAAAAGTGGCGAGGAAATATCCGGTGATGGCG
TGCTGGAGATTCTCCAGGACGGCTTCGGTTTCCTGCGCTCTGCAGATGCTTCCTACCTGGCCGGCCCGGATGACATCTAT
GTCTCCCCCAGCCAGATCCGCCGCTTCAACCTGCGTACCGGCGATACCATCGTTGGCAAGATCCGTCCACCCAAGGAAGG
CGAGCGTTATTTCGCTCTGCTCAAGGTCGACACGATCAACTACGACCGTCCGGAAAACGCCAAGAACAAGATTCTGTTCG
AAAACCTGACGCCGTTGTTCCCGACGATTCGCATGAAGATGGAAGCCGGTAACGGTTCCACCGAAGACCTGACCGGTCGT
GTGATCGATCTGTGCGCACCGATCGGTAAGGGCCAGCGTGGTCTGATCGTTGCTCCGCCGAAGGCGGGCAAGACCATCAT
GCTGCAGAACATCGCGTCGAACATCACGCGTAACAACCCGGAAGTGCATCTGATCGTTCTGCTGATCGACGAGCGCCCGG
AAGAAGTGACCGAGATGCAGCGCACCGTGCGCGGCGAAGTGGTCGCCTCGACGTTCGACGAGCCGCCAACCCGTCACGTA
CAGGTTGCCGAAATGGTGATCGAGAAGGCCAAGCGCCTGGTCGAGCACAAGAAAGACGTCGTTATCCTGCTCGACTCCAT
CACCCGTCTGGCCCGTGCCTACAACACCGTCATCCCGAGCTCCGGCAAGGTATTGACCGGTGGTGTCGACGCTCACGCAC
TCGAGAAGCCAAAGCGTTTCTTCGGTGCTGCGCGTAACATCGAAGAAGGCGGCTCGTTGACCATCATCGCCACCGCACTG
GTTGAAACCGGTTCGAAGATGGATGAAGTGATCTACGAAGAGTTCAAGGGTACCGGCAACATGGAACTGCCTCTGGATCG
CAAGATCGCAGAGAAGCGCGTGTTCCCGGCCATCAACATCAACCGCTCCGGCACACGCCGTGAAGAGCTGTTGACCGCTG
ATGACGAGCTGCAGCGCATGTGGATCCTGCGCAAGCTGCTGCACCCGATGGACGAAGTCGCGGCTATCGAGTTCCTCATC
GACAAGCTTAAGCAGACCAAGACCAACGACGAGTTCTTCCTGTCCATGAAACGCAAGTAA

Upstream 100 bases:

>100_bases
TTTCGATTTAGTACGCGATCCTGTCGCCTTTTCTGCGGCGCGGCCTCATTAAGCCAAAAGCTTAATTTCCCCCTCCATAC
ATGATTACGTCATTCCCCAT

Downstream 100 bases:

>100_bases
CTGGCAGGCTGAAACAAAAAATGGTGTCCTTCGGGGCACCATTTTTTTTGCCTGCATGCCGCCGATTAGCTGCCTTGACG
GGCTGGAGCAGGTACGATGT

Product: transcription termination factor Rho

Products: NA

Alternate protein names: ATP-dependent helicase Rho [H]

Number of amino acids: Translated: 419; Mature: 419

Protein sequence:

>419_residues
MNLTELKQKPITELLEMAEQAGIENMARSRKQDVIFSLLKRHAKSGEEISGDGVLEILQDGFGFLRSADASYLAGPDDIY
VSPSQIRRFNLRTGDTIVGKIRPPKEGERYFALLKVDTINYDRPENAKNKILFENLTPLFPTIRMKMEAGNGSTEDLTGR
VIDLCAPIGKGQRGLIVAPPKAGKTIMLQNIASNITRNNPEVHLIVLLIDERPEEVTEMQRTVRGEVVASTFDEPPTRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTIIATAL
VETGSKMDEVIYEEFKGTGNMELPLDRKIAEKRVFPAININRSGTRREELLTADDELQRMWILRKLLHPMDEVAAIEFLI
DKLKQTKTNDEFFLSMKRK

Sequences:

>Translated_419_residues
MNLTELKQKPITELLEMAEQAGIENMARSRKQDVIFSLLKRHAKSGEEISGDGVLEILQDGFGFLRSADASYLAGPDDIY
VSPSQIRRFNLRTGDTIVGKIRPPKEGERYFALLKVDTINYDRPENAKNKILFENLTPLFPTIRMKMEAGNGSTEDLTGR
VIDLCAPIGKGQRGLIVAPPKAGKTIMLQNIASNITRNNPEVHLIVLLIDERPEEVTEMQRTVRGEVVASTFDEPPTRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTIIATAL
VETGSKMDEVIYEEFKGTGNMELPLDRKIAEKRVFPAININRSGTRREELLTADDELQRMWILRKLLHPMDEVAAIEFLI
DKLKQTKTNDEFFLSMKRK
>Mature_419_residues
MNLTELKQKPITELLEMAEQAGIENMARSRKQDVIFSLLKRHAKSGEEISGDGVLEILQDGFGFLRSADASYLAGPDDIY
VSPSQIRRFNLRTGDTIVGKIRPPKEGERYFALLKVDTINYDRPENAKNKILFENLTPLFPTIRMKMEAGNGSTEDLTGR
VIDLCAPIGKGQRGLIVAPPKAGKTIMLQNIASNITRNNPEVHLIVLLIDERPEEVTEMQRTVRGEVVASTFDEPPTRHV
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALEKPKRFFGAARNIEEGGSLTIIATAL
VETGSKMDEVIYEEFKGTGNMELPLDRKIAEKRVFPAININRSGTRREELLTADDELQRMWILRKLLHPMDEVAAIEFLI
DKLKQTKTNDEFFLSMKRK

Specific function: Facilitates transcription termination by a mechanism that involves Rho binding to the nascent RNA, activation of Rho's RNA-dependent ATPase activity, and release of the mRNA from the DNA template [H]

COG id: COG1158

COG function: function code K; Transcription termination factor

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Rho family [H]

Homologues:

Organism=Escherichia coli, GI1790217, Length=418, Percent_Identity=79.9043062200957, Blast_Score=702, Evalue=0.0,

Paralogues:

None

Copy number: 300 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000194
- InterPro:   IPR003593
- InterPro:   IPR011129
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR011112
- InterPro:   IPR011113
- InterPro:   IPR004665 [H]

Pfam domain/function: PF00006 ATP-synt_ab; PF07498 Rho_N; PF07497 Rho_RNA_bind [H]

EC number: NA

Molecular weight: Translated: 47030; Mature: 47030

Theoretical pI: Translated: 7.75; Mature: 7.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLTELKQKPITELLEMAEQAGIENMARSRKQDVIFSLLKRHAKSGEEISGDGVLEILQD
CCHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSADASYLAGPDDIYVSPSQIRRFNLRTGDTIVGKIRPPKEGERYFALLKVDTIN
HHHHHHCCCCCCCCCCCCEEECHHHHHEEECCCCCEEEEECCCCCCCCEEEEEEEEEECC
YDRPENAKNKILFENLTPLFPTIRMKMEAGNGSTEDLTGRVIDLCAPIGKGQRGLIVAPP
CCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECC
KAGKTIMLQNIASNITRNNPEVHLIVLLIDERPEEVTEMQRTVRGEVVASTFDEPPTRHV
CCCCEEEHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALEKPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIIATALVETGSKMDEVIYEEFKGTGNMELPLDRKIAEKRVFPAINI
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEC
NRSGTRREELLTADDELQRMWILRKLLHPMDEVAAIEFLIDKLKQTKTNDEFFLSMKRK
CCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC
>Mature Secondary Structure
MNLTELKQKPITELLEMAEQAGIENMARSRKQDVIFSLLKRHAKSGEEISGDGVLEILQD
CCHHHHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHH
GFGFLRSADASYLAGPDDIYVSPSQIRRFNLRTGDTIVGKIRPPKEGERYFALLKVDTIN
HHHHHHCCCCCCCCCCCCEEECHHHHHEEECCCCCEEEEECCCCCCCCEEEEEEEEEECC
YDRPENAKNKILFENLTPLFPTIRMKMEAGNGSTEDLTGRVIDLCAPIGKGQRGLIVAPP
CCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHCCCCCCCCCEEEECC
KAGKTIMLQNIASNITRNNPEVHLIVLLIDERPEEVTEMQRTVRGEVVASTFDEPPTRHV
CCCCEEEHHHHHHHHCCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCCCHHH
QVAEMVIEKAKRLVEHKKDVVILLDSITRLARAYNTVIPSSGKVLTGGVDAHALEKPKRF
HHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHHHHHCCCCCCCEEECCCCHHHHHHHHHH
FGAARNIEEGGSLTIIATALVETGSKMDEVIYEEFKGTGNMELPLDRKIAEKRVFPAINI
HHHHCCCCCCCCEEEEEHHHHHCCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHCCCCEEC
NRSGTRREELLTADDELQRMWILRKLLHPMDEVAAIEFLIDKLKQTKTNDEFFLSMKRK
CCCCCHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10984043 [H]