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The map label for this gene is amiD [H]

Identifier: 66043535

GI number: 66043535

Start: 282046

End: 282897

Strand: Reverse

Name: amiD [H]

Synonym: Psyr_0265

Alternate gene names: 66043535

Gene position: 282897-282046 (Counterclockwise)

Preceding gene: 66043536

Following gene: 66043532

Centisome position: 4.64

GC content: 59.04

Gene sequence:

>852_bases
ATGGCTGGTGCCCCATGCTATTTGTATACATTTTGTTATGATGTTTCATTTCCTGCCGACGCTGCCGACCTCATGAAGCT
GTATTTATCCGCCTTTCTCATGCTCCTCCTCACTGCCTGTGCCAGCGGCCCAAGGCTGGACACCAGCCATCCTTCGGTAA
ATTTCGATGGCCGTGCTCAGTACGTGGTGATGCATTACACCTCGACGTCGCTGGAACACTCGCTGCAATTACTGACGCAT
GGCGAGGTCAGCGCCCACTACCTGATCGGTGACGACAGCAAAGCCACCATCTACAAGCTGGTCGATGAAAACGCGCGTGC
CTGGCATGCCGGCGAAAGCGAGTGGGAAGGCCGCACCTGGCTCAACTCCAGCTCGATCGGCATCGAGATCGTCAATCCGG
GTTTCAAGGAAACGCCGACAGGGCGCCTGTGGTATCCGTATACCGAAGCGCAGACCCAGTCGATCATTGTCCTGCTCAAG
GACATCGTCAAACGCAACAGGATCGACCCCAAACACATCATCGGTCATAGCGACATCGCCCCATTGCGCAAGCAGGACCC
GGGGCCATTGTTCCCGTGGAAACGCCTCGCTGCCGAAGGTCTGGGGAACTGGCCGGATGAACGCCAGGTTGCCCAGCGTC
AGGCACTGCTGGCAACTAACCTGCCGAGCATCACCTGGTTCCAGCAGCAGTTGGCGCGCCTGGGCTACAGCACCCCGCAG
ACCGGTGAGCTGGATGTCGCGACACGTCAGGTACTGGCCGCCTTCCAGATGCACTATCGCCCGGCACGCTTCGACGGCGA
GCCGGACGCACAGAGCGCGGCCATCCTGCAGGTGCTCAATCACTCGAATTGA

Upstream 100 bases:

>100_bases
ACACCGCCATCAAACGTGCCGACCAGGCGCTTTACAAGGCCAAGGAGAACGGTCGCAACCGCATCGAACAGGGCTGACTG
AAACATTTTGACCTGAACAA

Downstream 100 bases:

>100_bases
AACGTGTGAAGGCTGATACTCAGTCAACCGATGAGGGCCTGGTGCTCAAGCCCTCAGAGCGGTAACGCCATATAGAACTG
CGTGCCCTGCCCCGGCCGTG

Product: N-acetylmuramoyl-L-alanine amidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 283; Mature: 282

Protein sequence:

>283_residues
MAGAPCYLYTFCYDVSFPADAADLMKLYLSAFLMLLLTACASGPRLDTSHPSVNFDGRAQYVVMHYTSTSLEHSLQLLTH
GEVSAHYLIGDDSKATIYKLVDENARAWHAGESEWEGRTWLNSSSIGIEIVNPGFKETPTGRLWYPYTEAQTQSIIVLLK
DIVKRNRIDPKHIIGHSDIAPLRKQDPGPLFPWKRLAAEGLGNWPDERQVAQRQALLATNLPSITWFQQQLARLGYSTPQ
TGELDVATRQVLAAFQMHYRPARFDGEPDAQSAAILQVLNHSN

Sequences:

>Translated_283_residues
MAGAPCYLYTFCYDVSFPADAADLMKLYLSAFLMLLLTACASGPRLDTSHPSVNFDGRAQYVVMHYTSTSLEHSLQLLTH
GEVSAHYLIGDDSKATIYKLVDENARAWHAGESEWEGRTWLNSSSIGIEIVNPGFKETPTGRLWYPYTEAQTQSIIVLLK
DIVKRNRIDPKHIIGHSDIAPLRKQDPGPLFPWKRLAAEGLGNWPDERQVAQRQALLATNLPSITWFQQQLARLGYSTPQ
TGELDVATRQVLAAFQMHYRPARFDGEPDAQSAAILQVLNHSN
>Mature_282_residues
AGAPCYLYTFCYDVSFPADAADLMKLYLSAFLMLLLTACASGPRLDTSHPSVNFDGRAQYVVMHYTSTSLEHSLQLLTHG
EVSAHYLIGDDSKATIYKLVDENARAWHAGESEWEGRTWLNSSSIGIEIVNPGFKETPTGRLWYPYTEAQTQSIIVLLKD
IVKRNRIDPKHIIGHSDIAPLRKQDPGPLFPWKRLAAEGLGNWPDERQVAQRQALLATNLPSITWFQQQLARLGYSTPQT
GELDVATRQVLAAFQMHYRPARFDGEPDAQSAAILQVLNHSN

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=244, Percent_Identity=41.3934426229508, Blast_Score=181, Evalue=7e-47,
Organism=Escherichia coli, GI1786300, Length=119, Percent_Identity=39.4957983193277, Blast_Score=78, Evalue=6e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31671; Mature: 31540

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAGAPCYLYTFCYDVSFPADAADLMKLYLSAFLMLLLTACASGPRLDTSHPSVNFDGRAQ
CCCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
YVVMHYTSTSLEHSLQLLTHGEVSAHYLIGDDSKATIYKLVDENARAWHAGESEWEGRTW
EEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCEECCCCCCCCCCEE
LNSSSIGIEIVNPGFKETPTGRLWYPYTEAQTQSIIVLLKDIVKRNRIDPKHIIGHSDIA
ECCCCCEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
PLRKQDPGPLFPWKRLAAEGLGNWPDERQVAQRQALLATNLPSITWFQQQLARLGYSTPQ
CHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
TGELDVATRQVLAAFQMHYRPARFDGEPDAQSAAILQVLNHSN
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC
>Mature Secondary Structure 
AGAPCYLYTFCYDVSFPADAADLMKLYLSAFLMLLLTACASGPRLDTSHPSVNFDGRAQ
CCCCEEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCEE
YVVMHYTSTSLEHSLQLLTHGEVSAHYLIGDDSKATIYKLVDENARAWHAGESEWEGRTW
EEEEEECCCCHHHHHHHHCCCCCEEEEEECCCCCCEEEEECCCCCCEECCCCCCCCCCEE
LNSSSIGIEIVNPGFKETPTGRLWYPYTEAQTQSIIVLLKDIVKRNRIDPKHIIGHSDIA
ECCCCCEEEEECCCCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHCCCCCCC
PLRKQDPGPLFPWKRLAAEGLGNWPDERQVAQRQALLATNLPSITWFQQQLARLGYSTPQ
CHHCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCC
TGELDVATRQVLAAFQMHYRPARFDGEPDAQSAAILQVLNHSN
CCCCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]