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The map label for this gene is pleD [H]

Identifier: 66043536

GI number: 66043536

Start: 282921

End: 284993

Strand: Reverse

Name: pleD [H]

Synonym: Psyr_0266

Alternate gene names: 66043536

Gene position: 284993-282921 (Counterclockwise)

Preceding gene: 66043537

Following gene: 66043535

Centisome position: 4.68

GC content: 62.57

Gene sequence:

>2073_bases
ATGAGCGACGACGCGGAGCGTTGGAAAGAGAAATACCTCAAAGGGATCGAGCAACAGGACACGCTCGAAAAGCGCTGGGA
TGCGCGGCTCGATTTGTTGCGACGCGGTCTGGTGCGCAGCAGTCTGGCGGCAGAAGGCTCTGACCGCGCCGTCGATGAAT
GCATGAAGGAAATGCGCGAAATCGTCCGCAAGGACGATATGGACGCGGGCCTGGCAGCCCTGATCCCGCGCCTCGAAAAA
GCCGTACTGGACTCTGAACAACGCCGTGAAGTCAGGGCCGCGCAAATCAGCTCGGCGCTGACCGCACTGGTTGCACAGCT
GCAGTCACTGCCGCTGCCACGCGACGTACGCAAGCCGCTCAAGCGCTTTGCCAAGGAGCTTGAAGAGCGCGCCTCCCAGG
CCCGTGAGCTGCCCCTGCTGCTTGGCGAGTTGAGCAATTTGCAGGGCCAGGCCCTGACCCAGATTGAAAAACCTGAAGAA
GGTCCCCGTCAGGGGCTGCTGCAACGCCTGTTCGGTTCGCGCGAGGGTCACGGCCACGAGCCTCATCCTGAGGCGGCTCA
TCCCGAAGACGCCGCCTCCTCGGCGCCGGAATCGCATGAACCCGACGTCGCGCCGCAAGCAGCGAATACTTACGCCCCGC
CAGACGCGTCTCCTGTCGAAGCCGAGGCCGTTGATTCCACTGCCGCAGCCGCCGTTGCCGCAGACAGCACGCCGCCAACG
CCGGCCCCGGAAACCGATGCGCCTGCCGCAGTGATACCCGCCGTTGCGACGCCTGAAGAACCTGCGCCATCGACCACCGC
TGCGGACATTGCTCCAGCGCCAACAGAGGCCGTCGCCGAACCTGCACCGATCACTGTCGAACCCATCGATTTCGCGGATC
TCCTGGCGCCTGTCCCATCAGTGCCCTACCAGCAGCGACCCGCGCCAATCGACGTGCCAGTTGAAGAACCCGACGCAGAG
GCCGAGCAGCAGCCCGAGTCGGCAGAAGGCGAAGAAGGTTACGCGCTACCCTCCTCACCAGAGCCCAGCTATAGCTCGGT
GGCGGCACACATTGAACAGACCCTGCTGGGCCTGCTGGACGACCTGACCCTGTCAGAGCACTTTCGCCCCCAGGTCGAGG
ACATGCAGTTACGCTTGAAACATGGGCTTAACTGGTACGAATTGCTGCCGATCCTCGACGATCTGGCAGTGTTGATGCTG
GCGATCAACAACGGCGGCCAACAGGAGTTCGGCACTTACCTCAAGCAGCTCAATGAACGGCTCGAATCGTTTCAGAGCCA
CCTGCAGGCCGCCAGTGAAGATCACGCCGAGGACCAGTCCACCGCGCGCGACCTGAACGAACAGTTGCGCGAACAGGTGG
GCGGCCTGCAAAGCAGTGTTCAGGACGCCTCCGATCTGCCCAGCCTCAAGCGGGTGCTCGACAATCGGCTTGAGGCCCTG
CTCGGCACCATGGATCACTACCAGCTCAAGCGCGATGCCCGCGAGCGCGAAGTGGCCTCGCGCCTGCAAGGTCTGGCGGC
CCGCGTCGCGAGCATGGAGCAGGAGGCCCTGGGTTTCAGGACTCACCTGGAGGAACAGCGTCAAAAGGCCCTGATCGACC
CGCTTACCGGACTGCCCAATCGTGCCGCCTGGGGCGAGCGCCTTGAACGGGAGATGGCCCGCTGGCACGAAGAAAAAAAC
AGCCTGCTGGTGTGCATTCTGGACCTGGACCATTTCAAACGCATCAACGATGGCTACGGGCACCTGGCTGGCGACAAGGT
ATTGAAGATCGTCGCCAATGTCCTCAGCAAACGCCTGCGCGCCGATGACTTCATGGCACGGTTTGGCGGCGAAGAATTTG
TCATGCTGCTGCCTGCGACACCTCCTGCCACCGGGCTGAAACTGCTCGATGAACTGCGCGCCGCCGTCGAGTCATGCCCT
TTCCACTTCAAGGGCGAACGAGTCACCATCACTGTATCCATCGGCATGACGGCCTTTCGTCCGTCAGAGCGGAGCGACAC
CGCCATCAAACGTGCCGACCAGGCGCTTTACAAGGCCAAGGAGAACGGTCGCAACCGCATCGAACAGGGCTGA

Upstream 100 bases:

>100_bases
CGCAACCCATTTCCGATCACCTGCCTGTCGCTGTCGAGATTCGCCTGCCAGACTCATTGCGTGGGGACTCACTGCCTGTG
CCCTCTGGTGGAAGCCTTGC

Downstream 100 bases:

>100_bases
CTGAAACATTTTGACCTGAACAAATGGCTGGTGCCCCATGCTATTTGTATACATTTTGTTATGATGTTTCATTTCCTGCC
GACGCTGCCGACCTCATGAA

Product: GGDEF

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 690; Mature: 689

Protein sequence:

>690_residues
MSDDAERWKEKYLKGIEQQDTLEKRWDARLDLLRRGLVRSSLAAEGSDRAVDECMKEMREIVRKDDMDAGLAALIPRLEK
AVLDSEQRREVRAAQISSALTALVAQLQSLPLPRDVRKPLKRFAKELEERASQARELPLLLGELSNLQGQALTQIEKPEE
GPRQGLLQRLFGSREGHGHEPHPEAAHPEDAASSAPESHEPDVAPQAANTYAPPDASPVEAEAVDSTAAAAVAADSTPPT
PAPETDAPAAVIPAVATPEEPAPSTTAADIAPAPTEAVAEPAPITVEPIDFADLLAPVPSVPYQQRPAPIDVPVEEPDAE
AEQQPESAEGEEGYALPSSPEPSYSSVAAHIEQTLLGLLDDLTLSEHFRPQVEDMQLRLKHGLNWYELLPILDDLAVLML
AINNGGQQEFGTYLKQLNERLESFQSHLQAASEDHAEDQSTARDLNEQLREQVGGLQSSVQDASDLPSLKRVLDNRLEAL
LGTMDHYQLKRDAREREVASRLQGLAARVASMEQEALGFRTHLEEQRQKALIDPLTGLPNRAAWGERLEREMARWHEEKN
SLLVCILDLDHFKRINDGYGHLAGDKVLKIVANVLSKRLRADDFMARFGGEEFVMLLPATPPATGLKLLDELRAAVESCP
FHFKGERVTITVSIGMTAFRPSERSDTAIKRADQALYKAKENGRNRIEQG

Sequences:

>Translated_690_residues
MSDDAERWKEKYLKGIEQQDTLEKRWDARLDLLRRGLVRSSLAAEGSDRAVDECMKEMREIVRKDDMDAGLAALIPRLEK
AVLDSEQRREVRAAQISSALTALVAQLQSLPLPRDVRKPLKRFAKELEERASQARELPLLLGELSNLQGQALTQIEKPEE
GPRQGLLQRLFGSREGHGHEPHPEAAHPEDAASSAPESHEPDVAPQAANTYAPPDASPVEAEAVDSTAAAAVAADSTPPT
PAPETDAPAAVIPAVATPEEPAPSTTAADIAPAPTEAVAEPAPITVEPIDFADLLAPVPSVPYQQRPAPIDVPVEEPDAE
AEQQPESAEGEEGYALPSSPEPSYSSVAAHIEQTLLGLLDDLTLSEHFRPQVEDMQLRLKHGLNWYELLPILDDLAVLML
AINNGGQQEFGTYLKQLNERLESFQSHLQAASEDHAEDQSTARDLNEQLREQVGGLQSSVQDASDLPSLKRVLDNRLEAL
LGTMDHYQLKRDAREREVASRLQGLAARVASMEQEALGFRTHLEEQRQKALIDPLTGLPNRAAWGERLEREMARWHEEKN
SLLVCILDLDHFKRINDGYGHLAGDKVLKIVANVLSKRLRADDFMARFGGEEFVMLLPATPPATGLKLLDELRAAVESCP
FHFKGERVTITVSIGMTAFRPSERSDTAIKRADQALYKAKENGRNRIEQG
>Mature_689_residues
SDDAERWKEKYLKGIEQQDTLEKRWDARLDLLRRGLVRSSLAAEGSDRAVDECMKEMREIVRKDDMDAGLAALIPRLEKA
VLDSEQRREVRAAQISSALTALVAQLQSLPLPRDVRKPLKRFAKELEERASQARELPLLLGELSNLQGQALTQIEKPEEG
PRQGLLQRLFGSREGHGHEPHPEAAHPEDAASSAPESHEPDVAPQAANTYAPPDASPVEAEAVDSTAAAAVAADSTPPTP
APETDAPAAVIPAVATPEEPAPSTTAADIAPAPTEAVAEPAPITVEPIDFADLLAPVPSVPYQQRPAPIDVPVEEPDAEA
EQQPESAEGEEGYALPSSPEPSYSSVAAHIEQTLLGLLDDLTLSEHFRPQVEDMQLRLKHGLNWYELLPILDDLAVLMLA
INNGGQQEFGTYLKQLNERLESFQSHLQAASEDHAEDQSTARDLNEQLREQVGGLQSSVQDASDLPSLKRVLDNRLEALL
GTMDHYQLKRDAREREVASRLQGLAARVASMEQEALGFRTHLEEQRQKALIDPLTGLPNRAAWGERLEREMARWHEEKNS
LLVCILDLDHFKRINDGYGHLAGDKVLKIVANVLSKRLRADDFMARFGGEEFVMLLPATPPATGLKLLDELRAAVESCPF
HFKGERVTITVSIGMTAFRPSERSDTAIKRADQALYKAKENGRNRIEQG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI145693134, Length=166, Percent_Identity=34.9397590361446, Blast_Score=103, Evalue=3e-23,
Organism=Escherichia coli, GI87081881, Length=172, Percent_Identity=39.5348837209302, Blast_Score=102, Evalue=6e-23,
Organism=Escherichia coli, GI1787802, Length=169, Percent_Identity=36.6863905325444, Blast_Score=102, Evalue=7e-23,
Organism=Escherichia coli, GI1787262, Length=243, Percent_Identity=31.6872427983539, Blast_Score=101, Evalue=2e-22,
Organism=Escherichia coli, GI1787816, Length=162, Percent_Identity=36.4197530864198, Blast_Score=99, Evalue=9e-22,
Organism=Escherichia coli, GI1787541, Length=170, Percent_Identity=37.0588235294118, Blast_Score=96, Evalue=9e-21,
Organism=Escherichia coli, GI1786584, Length=175, Percent_Identity=33.7142857142857, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI87082007, Length=165, Percent_Identity=36.3636363636364, Blast_Score=85, Evalue=1e-17,
Organism=Escherichia coli, GI1788381, Length=169, Percent_Identity=30.7692307692308, Blast_Score=82, Evalue=2e-16,
Organism=Escherichia coli, GI1788956, Length=271, Percent_Identity=30.6273062730627, Blast_Score=77, Evalue=3e-15,
Organism=Escherichia coli, GI1787056, Length=166, Percent_Identity=31.9277108433735, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1788085, Length=207, Percent_Identity=28.5024154589372, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI87081974, Length=170, Percent_Identity=27.0588235294118, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87081921, Length=185, Percent_Identity=26.4864864864865, Blast_Score=67, Evalue=4e-12,
Organism=Escherichia coli, GI87081977, Length=204, Percent_Identity=29.4117647058824, Blast_Score=66, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 75947; Mature: 75816

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDDAERWKEKYLKGIEQQDTLEKRWDARLDLLRRGLVRSSLAAEGSDRAVDECMKEMRE
CCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IVRKDDMDAGLAALIPRLEKAVLDSEQRREVRAAQISSALTALVAQLQSLPLPRDVRKPL
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KRFAKELEERASQARELPLLLGELSNLQGQALTQIEKPEEGPRQGLLQRLFGSREGHGHE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
PHPEAAHPEDAASSAPESHEPDVAPQAANTYAPPDASPVEAEAVDSTAAAAVAADSTPPT
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
PAPETDAPAAVIPAVATPEEPAPSTTAADIAPAPTEAVAEPAPITVEPIDFADLLAPVPS
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEECCCCHHHHHCCCCC
VPYQQRPAPIDVPVEEPDAEAEQQPESAEGEEGYALPSSPEPSYSSVAAHIEQTLLGLLD
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DLTLSEHFRPQVEDMQLRLKHGLNWYELLPILDDLAVLMLAINNGGQQEFGTYLKQLNER
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LESFQSHLQAASEDHAEDQSTARDLNEQLREQVGGLQSSVQDASDLPSLKRVLDNRLEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LGTMDHYQLKRDAREREVASRLQGLAARVASMEQEALGFRTHLEEQRQKALIDPLTGLPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RAAWGERLEREMARWHEEKNSLLVCILDLDHFKRINDGYGHLAGDKVLKIVANVLSKRLR
CHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ADDFMARFGGEEFVMLLPATPPATGLKLLDELRAAVESCPFHFKGERVTITVSIGMTAFR
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEECCEECC
PSERSDTAIKRADQALYKAKENGRNRIEQG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SDDAERWKEKYLKGIEQQDTLEKRWDARLDLLRRGLVRSSLAAEGSDRAVDECMKEMRE
CCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
IVRKDDMDAGLAALIPRLEKAVLDSEQRREVRAAQISSALTALVAQLQSLPLPRDVRKPL
HHHHCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHH
KRFAKELEERASQARELPLLLGELSNLQGQALTQIEKPEEGPRQGLLQRLFGSREGHGHE
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCC
PHPEAAHPEDAASSAPESHEPDVAPQAANTYAPPDASPVEAEAVDSTAAAAVAADSTPPT
CCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCC
PAPETDAPAAVIPAVATPEEPAPSTTAADIAPAPTEAVAEPAPITVEPIDFADLLAPVPS
CCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCEECCCCHHHHHCCCCC
VPYQQRPAPIDVPVEEPDAEAEQQPESAEGEEGYALPSSPEPSYSSVAAHIEQTLLGLLD
CCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHH
DLTLSEHFRPQVEDMQLRLKHGLNWYELLPILDDLAVLMLAINNGGQQEFGTYLKQLNER
HHHHHHHCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHH
LESFQSHLQAASEDHAEDQSTARDLNEQLREQVGGLQSSVQDASDLPSLKRVLDNRLEAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
LGTMDHYQLKRDAREREVASRLQGLAARVASMEQEALGFRTHLEEQRQKALIDPLTGLPN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
RAAWGERLEREMARWHEEKNSLLVCILDLDHFKRINDGYGHLAGDKVLKIVANVLSKRLR
CHHHHHHHHHHHHHHHHCCCCEEEEEECHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ADDFMARFGGEEFVMLLPATPPATGLKLLDELRAAVESCPFHFKGERVTITVSIGMTAFR
HHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHHHHHHHCCCEECCCEEEEEEEECCEECC
PSERSDTAIKRADQALYKAKENGRNRIEQG
CCCCCHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA