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The map label for this gene is puuB [H]

Identifier: 66043502

GI number: 66043502

Start: 246686

End: 247999

Strand: Reverse

Name: puuB [H]

Synonym: Psyr_0232

Alternate gene names: 66043502

Gene position: 247999-246686 (Counterclockwise)

Preceding gene: 66043504

Following gene: 66043500

Centisome position: 4.07

GC content: 62.4

Gene sequence:

>1314_bases
ATGAACGCCCGCGTTCAGAACACGCCCCGTCAAGATCAGCACGTTGCCTCCTATTACGCCGCCAGCAGTCATGCACAAGC
GGATCATCCTGCGCTGCAAGGTGAGGTGAAGGTCGATGTGTGCGTGGTCGGCGGTGGATTCTCCGGCCTCAATACCGCCA
TCGAACTGGCCGAGCGCGGGTTCAGCGTTGCGTTGCTCGAAGCGCGCAAGATCGGTTGGGGGGCCAGCGGGCGCAATGGC
GGCCAGTTGATTCGCGGGGTCGGTCATGGTGTCGAACAGTTCGCCAATGTCATCGGCAGCGAAGGCGTGCGGCAGCTCAA
ACTGCTGGGCATCGAGGCCGTCGAGATCGTGCGTCAGCGCATCGAGCGGTACGCTATCGATTGCGACCTGAAGTGGGGTT
ATTGCGATCTGGCCAACAAGCCCCGCGACCTGCACAGCCTTGCTGAAGATGCAGAAGAGTTGCGCAGCCTGGGTTATCGC
CATGCACTGCAGTTGCTGCAACCGGAAGAGATGCACAGCGTGGTGGGCTCCTCGCGCTATGTCGGCGGCCTGATCGACAT
GGGCTCGGGGCATTTGCATCCGTTGAACCTTGCCTTGGGCGAAGCGGCGGTAGCGCAGTCGCTCGGCGTACGGCTGTTCG
AACATTCAGCGGTGACGAGCATCGACTACGGCCCGCAGGTCAGGGTGCATACGGCTGCCGGTTCGGTGCAGGCGCAGACC
CTGGTGCTGGGTTGCAACGCCTACCTCAATGACTTGAATCCGGAAATCAGCGGCAAGGTGTTGCCTGCCGGCAGCTACAT
CATCGCCACCGAGCCGTTGAGCGAAGCGCAGGCCCGTGAAGTGTTGCCGCAGGACATGGCGGTATGTGATCAGCGGGTGA
CGGTGGATTACTACAGGCTGTCTGCCGACCGCCGCTTGCTGTTCGGCGGTGCCTGCCATTACTCGGGGCGTGACCCTCAG
GACATCGCCGCGTACATGCGGCCGAAGATGCTCGATGTGTTCCCGCAACTGGCCGCCGTGAAGATCGATTACCAGTGGGG
CGGGATGATCGGCATCGGCGCCAATCGCCTGCCGCAGATAGGCAGGCTCAAGCAGCACCCCAACGTATTTTATGCGCAGG
CCTACTCGGGCCACGGTCTGAATGCCACGCATCTCGCGGGCCGGCTGCTGGCCGAAGCCATCGCCGGGCAACACAGCGAT
GGTTTCGAGTTGTTCGCCAAAGTGCCGCACATGACCTTTCCCGGCGGCAAGCACTTGCGCTCGCCTCTGCTGGCATTGGG
CATGCTCTGGCACCGTCTCAAAGAGCTGCGCTAG

Upstream 100 bases:

>100_bases
CGACCACTCATGCACAAGGCCGAGAGTTTGCCTCAGTTGGCAGGCTCATGACAGCGGCTTATCCTCCCGACACGCCATCC
GCCACCAACAGGACACGCTC

Downstream 100 bases:

>100_bases
GCTCAGGGCCTCCAGAACGGCTTCATGGCCTCGACCCGCGCCTGGCGGAAATCCAGGCCAATGTCGCGCAACTGATCGTC
GGTCAGTGTCAGCAAGCTGC

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 437; Mature: 437

Protein sequence:

>437_residues
MNARVQNTPRQDQHVASYYAASSHAQADHPALQGEVKVDVCVVGGGFSGLNTAIELAERGFSVALLEARKIGWGASGRNG
GQLIRGVGHGVEQFANVIGSEGVRQLKLLGIEAVEIVRQRIERYAIDCDLKWGYCDLANKPRDLHSLAEDAEELRSLGYR
HALQLLQPEEMHSVVGSSRYVGGLIDMGSGHLHPLNLALGEAAVAQSLGVRLFEHSAVTSIDYGPQVRVHTAAGSVQAQT
LVLGCNAYLNDLNPEISGKVLPAGSYIIATEPLSEAQAREVLPQDMAVCDQRVTVDYYRLSADRRLLFGGACHYSGRDPQ
DIAAYMRPKMLDVFPQLAAVKIDYQWGGMIGIGANRLPQIGRLKQHPNVFYAQAYSGHGLNATHLAGRLLAEAIAGQHSD
GFELFAKVPHMTFPGGKHLRSPLLALGMLWHRLKELR

Sequences:

>Translated_437_residues
MNARVQNTPRQDQHVASYYAASSHAQADHPALQGEVKVDVCVVGGGFSGLNTAIELAERGFSVALLEARKIGWGASGRNG
GQLIRGVGHGVEQFANVIGSEGVRQLKLLGIEAVEIVRQRIERYAIDCDLKWGYCDLANKPRDLHSLAEDAEELRSLGYR
HALQLLQPEEMHSVVGSSRYVGGLIDMGSGHLHPLNLALGEAAVAQSLGVRLFEHSAVTSIDYGPQVRVHTAAGSVQAQT
LVLGCNAYLNDLNPEISGKVLPAGSYIIATEPLSEAQAREVLPQDMAVCDQRVTVDYYRLSADRRLLFGGACHYSGRDPQ
DIAAYMRPKMLDVFPQLAAVKIDYQWGGMIGIGANRLPQIGRLKQHPNVFYAQAYSGHGLNATHLAGRLLAEAIAGQHSD
GFELFAKVPHMTFPGGKHLRSPLLALGMLWHRLKELR
>Mature_437_residues
MNARVQNTPRQDQHVASYYAASSHAQADHPALQGEVKVDVCVVGGGFSGLNTAIELAERGFSVALLEARKIGWGASGRNG
GQLIRGVGHGVEQFANVIGSEGVRQLKLLGIEAVEIVRQRIERYAIDCDLKWGYCDLANKPRDLHSLAEDAEELRSLGYR
HALQLLQPEEMHSVVGSSRYVGGLIDMGSGHLHPLNLALGEAAVAQSLGVRLFEHSAVTSIDYGPQVRVHTAAGSVQAQT
LVLGCNAYLNDLNPEISGKVLPAGSYIIATEPLSEAQAREVLPQDMAVCDQRVTVDYYRLSADRRLLFGGACHYSGRDPQ
DIAAYMRPKMLDVFPQLAAVKIDYQWGGMIGIGANRLPQIGRLKQHPNVFYAQAYSGHGLNATHLAGRLLAEAIAGQHSD
GFELFAKVPHMTFPGGKHLRSPLLALGMLWHRLKELR

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=424, Percent_Identity=44.3396226415094, Blast_Score=366, Evalue=1e-102,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 47469; Mature: 47469

Theoretical pI: Translated: 7.33; Mature: 7.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNARVQNTPRQDQHVASYYAASSHAQADHPALQGEVKVDVCVVGGGFSGLNTAIELAERG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCC
FSVALLEARKIGWGASGRNGGQLIRGVGHGVEQFANVIGSEGVRQLKLLGIEAVEIVRQR
CEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IERYAIDCDLKWGYCDLANKPRDLHSLAEDAEELRSLGYRHALQLLQPEEMHSVVGSSRY
HHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCC
VGGLIDMGSGHLHPLNLALGEAAVAQSLGVRLFEHSAVTSIDYGPQVRVHTAAGSVQAQT
CCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCEEEE
LVLGCNAYLNDLNPEISGKVLPAGSYIIATEPLSEAQAREVLPQDMAVCDQRVTVDYYRL
EEEECHHHHHCCCCCCCCEEECCCCEEEEECCHHHHHHHHHCHHHHHHHCCCCEEEEEEE
SADRRLLFGGACHYSGRDPQDIAAYMRPKMLDVFPQLAAVKIDYQWGGMIGIGANRLPQI
CCCCEEEEECEECCCCCCHHHHHHHHCHHHHHHHCCCEEEEEEEEECCEEECCCCCCCCH
GRLKQHPNVFYAQAYSGHGLNATHLAGRLLAEAIAGQHSDGFELFAKVPHMTFPGGKHLR
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHH
SPLLALGMLWHRLKELR
HHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNARVQNTPRQDQHVASYYAASSHAQADHPALQGEVKVDVCVVGGGFSGLNTAIELAERG
CCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEECCCHHHHHHHHHHHCC
FSVALLEARKIGWGASGRNGGQLIRGVGHGVEQFANVIGSEGVRQLKLLGIEAVEIVRQR
CEEEEEEHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
IERYAIDCDLKWGYCDLANKPRDLHSLAEDAEELRSLGYRHALQLLQPEEMHSVVGSSRY
HHHHEEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHCCHHHHHHHHCCCCC
VGGLIDMGSGHLHPLNLALGEAAVAQSLGVRLFEHSAVTSIDYGPQVRVHTAAGSVQAQT
CCCEEECCCCCCEEEHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEEECCCCCEEEE
LVLGCNAYLNDLNPEISGKVLPAGSYIIATEPLSEAQAREVLPQDMAVCDQRVTVDYYRL
EEEECHHHHHCCCCCCCCEEECCCCEEEEECCHHHHHHHHHCHHHHHHHCCCCEEEEEEE
SADRRLLFGGACHYSGRDPQDIAAYMRPKMLDVFPQLAAVKIDYQWGGMIGIGANRLPQI
CCCCEEEEECEECCCCCCHHHHHHHHCHHHHHHHCCCEEEEEEEEECCEEECCCCCCCCH
GRLKQHPNVFYAQAYSGHGLNATHLAGRLLAEAIAGQHSDGFELFAKVPHMTFPGGKHLR
HHHHCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCCCHHHH
SPLLALGMLWHRLKELR
HHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]