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The map label for this gene is pyrE [H]

Identifier: 66043486

GI number: 66043486

Start: 229459

End: 230100

Strand: Direct

Name: pyrE [H]

Synonym: Psyr_0216

Alternate gene names: 66043486

Gene position: 229459-230100 (Clockwise)

Preceding gene: 66043484

Following gene: 66043487

Centisome position: 3.77

GC content: 60.75

Gene sequence:

>642_bases
ATGCAGGCGTATCAACGCGATTTCATTCGTTTTGCCATCGATCGCGGTGTTCTGCGCTTCGGTGAGTTCACTCTCAAGTC
CGGGCGCACCAGCCCGTACTTCTTCAATGCGGGGCTGTTCAACACCGGTTCCGCACTGGCACAACTGGGGCGTTTCTACG
CAGCCGCCGTGGTTGAAAGCGGTATTGCCTTCGACGTTCTGTTCGGTCCGGCCTACAAGGGCATTCCGCTCGCATCGGCC
ACTGCCGTGGCGCTGGCCGAGCATCATGACCGTGATCTGCCGTGGTGTTTCAACCGCAAGGAAGCCAAGGCGCACGGCGA
GGGCGGCAGTCTGGTGGGCTCGCCGCTGGCGGGGAATGTGCTGATTATCGACGACGTGATCACTGCGGGCACCGCGATCC
GTGAGGTCATGCAGATCATCAAGGACCAGGATGCGACAGCTGCGGGCGTGCTGATCGCGCTCAATCGTCAGGAGCGCGGC
AATGGCGAGCTGTCGGCGATTCAGGAAGTCGAACGTGACTTCGGGATTCCGGTGGTGAGTATCGTGTCGCTGAATCAGGT
GCTCGAGTTCCTGGCCGACGACCCGCAGCTCAAGCAGCATCTGCCTGCTGTCGAAGCCTATCGCGCGCAATTCGGAATCT
GA

Upstream 100 bases:

>100_bases
CACACTGATGATCCGCATAAATGGCCCCAAAAATCACGTGCGTGTATGATACCCGAGCTCTACCTAATTAGCTAAATCCG
TGGTATTCGGAGCTTTTTTC

Downstream 100 bases:

>100_bases
GGACGTGCCTATGGCAGTCGGCAGCAGGGCACGGTTTTCGGTTTCAGGCACGCTGAGCGCTTTGCGGGCCTTGCTGGCTG
TCGTCATGCTTGTGCCCATG

Product: orotate phosphoribosyltransferase

Products: NA

Alternate protein names: OPRT; OPRTase [H]

Number of amino acids: Translated: 213; Mature: 213

Protein sequence:

>213_residues
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNTGSALAQLGRFYAAAVVESGIAFDVLFGPAYKGIPLASA
TAVALAEHHDRDLPWCFNRKEAKAHGEGGSLVGSPLAGNVLIIDDVITAGTAIREVMQIIKDQDATAAGVLIALNRQERG
NGELSAIQEVERDFGIPVVSIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGI

Sequences:

>Translated_213_residues
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNTGSALAQLGRFYAAAVVESGIAFDVLFGPAYKGIPLASA
TAVALAEHHDRDLPWCFNRKEAKAHGEGGSLVGSPLAGNVLIIDDVITAGTAIREVMQIIKDQDATAAGVLIALNRQERG
NGELSAIQEVERDFGIPVVSIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGI
>Mature_213_residues
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNTGSALAQLGRFYAAAVVESGIAFDVLFGPAYKGIPLASA
TAVALAEHHDRDLPWCFNRKEAKAHGEGGSLVGSPLAGNVLIIDDVITAGTAIREVMQIIKDQDATAAGVLIALNRQERG
NGELSAIQEVERDFGIPVVSIVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGI

Specific function: Catalyzes the transfer of a ribosyl phosphate group from 5-phosphoribose 1-diphosphate to orotate, leading to the formation of orotidine monophosphate (OMP) [H]

COG id: COG0461

COG function: function code F; Orotate phosphoribosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the purine/pyrimidine phosphoribosyltransferase family. PyrE subfamily [H]

Homologues:

Organism=Homo sapiens, GI4507835, Length=180, Percent_Identity=27.7777777777778, Blast_Score=81, Evalue=8e-16,
Organism=Escherichia coli, GI1790073, Length=213, Percent_Identity=68.5446009389671, Blast_Score=314, Evalue=3e-87,
Organism=Caenorhabditis elegans, GI17508631, Length=164, Percent_Identity=28.6585365853659, Blast_Score=77, Evalue=8e-15,
Organism=Caenorhabditis elegans, GI17554964, Length=191, Percent_Identity=28.7958115183246, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323530, Length=199, Percent_Identity=52.2613065326633, Blast_Score=201, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6323927, Length=221, Percent_Identity=46.606334841629, Blast_Score=192, Evalue=2e-50,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004467
- InterPro:   IPR023031
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00156 Pribosyltran [H]

EC number: =2.4.2.10 [H]

Molecular weight: Translated: 23037; Mature: 23037

Theoretical pI: Translated: 5.29; Mature: 5.29

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNTGSALAQLGRFYAAAVVES
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIAFDVLFGPAYKGIPLASATAVALAEHHDRDLPWCFNRKEAKAHGEGGSLVGSPLAGNV
CCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEECCCCCCCE
LIIDDVITAGTAIREVMQIIKDQDATAAGVLIALNRQERGNGELSAIQEVERDFGIPVVS
EEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHH
IVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGI
HHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCC
>Mature Secondary Structure
MQAYQRDFIRFAIDRGVLRFGEFTLKSGRTSPYFFNAGLFNTGSALAQLGRFYAAAVVES
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHHHHHHHHHHHHHHC
GIAFDVLFGPAYKGIPLASATAVALAEHHDRDLPWCFNRKEAKAHGEGGSLVGSPLAGNV
CCEEEEEECCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCHHHCCCCCCCEECCCCCCCE
LIIDDVITAGTAIREVMQIIKDQDATAAGVLIALNRQERGNGELSAIQEVERDFGIPVVS
EEEECHHHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHCCCHHH
IVSLNQVLEFLADDPQLKQHLPAVEAYRAQFGI
HHHHHHHHHHHCCCHHHHHHCCHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA