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The map label for this gene is dapF [H]

Identifier: 66043453

GI number: 66043453

Start: 196313

End: 197143

Strand: Direct

Name: dapF [H]

Synonym: Psyr_0183

Alternate gene names: 66043453

Gene position: 196313-197143 (Clockwise)

Preceding gene: 66043452

Following gene: 66043454

Centisome position: 3.22

GC content: 61.01

Gene sequence:

>831_bases
ATGCTGCTGCGTTTTACCAAGATGCACGGGCTGGGTAACGACTTCATGGTCCTGGACCTTGTGAGTCAGCACGCGCATAT
CTTGCCCAAGCACGCCAAACAATGGGGCGACCGCCACACCGGTATCGGTTTCGATCAGCTTTTGCTGGTCGAGGCGCCGA
ACAACCCGGATGTGGACTTTCGCTATCGAATCTTCAATTCCGACGGCTCCGAAGTCGAACAGTGCGGCAACGGCGCGCGC
TGCTTTGCCCGCTTTGTGCTCGACAAGCGTCTGACGGCCAAGAAGCAGATACGCGTCGAGACCAAGAGCGGCATCATCGA
ACTGGATATCCGCAGCGACGGGCAGATCAGCGTCGATATGGGGCCGCCCAGGTTTGTCCCTGAAGAGATTCCCTTCGAAG
CAGCGGAGCAGGCTACCTGTTACACCGTTGACGTCGACGGTCAGCATGTCGACATGGCGGCTGTGTCCATGGGCAATCCG
CACGCTGTGCTGCGGGTCGATGACATCAATAACGCACCGGTGCATGAACTGGGGCCGAAGATCGAGCATCACCCGCGCTT
TCCGGCCCGGGTCAACGTCGGCTTTCTCCACGTCGTGGACCGTCAGCGGGCACAGCTGCGTGTCTGGGAACGCGGTGCCG
GGGAAACCCAGGCGTGCGGCACCGGCGCATGTGCGGCAGCTGTCGCTGCCATCAGTCAGGGCTGGATGGACTCGCCGTTG
CTGATCGACCTGCCCGGAGGGCGCCTGTCCATCGAATGGGCTGGGCCGGGACATTCTGTCATGATGACCGGCCCCGCAGT
CAGGGTTTATGAAGGCCAAGTTCGTCTTTGA

Upstream 100 bases:

>100_bases
CCGAGGTACTGGTCGACGGTTCCCAGGCTTTTGAAGTGCGTCGTCGCGAAACCGTGGCCGAGCTGTTCGCTGGCGAAAGC
CTGCTGCCGGAGTAATCCCC

Downstream 100 bases:

>100_bases
GTGAGTAAGTACCATGACCGATCAACCTCCCGCTTCAACAGGCACACCCTGCGAATCGCCTGTCGATAGCGCAGCGCCAG
ACCTTGAAGCAGAGGCGGTC

Product: diaminopimelate epimerase

Products: NA

Alternate protein names: DAP epimerase [H]

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MLLRFTKMHGLGNDFMVLDLVSQHAHILPKHAKQWGDRHTGIGFDQLLLVEAPNNPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVLDKRLTAKKQIRVETKSGIIELDIRSDGQISVDMGPPRFVPEEIPFEAAEQATCYTVDVDGQHVDMAAVSMGNP
HAVLRVDDINNAPVHELGPKIEHHPRFPARVNVGFLHVVDRQRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGHSVMMTGPAVRVYEGQVRL

Sequences:

>Translated_276_residues
MLLRFTKMHGLGNDFMVLDLVSQHAHILPKHAKQWGDRHTGIGFDQLLLVEAPNNPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVLDKRLTAKKQIRVETKSGIIELDIRSDGQISVDMGPPRFVPEEIPFEAAEQATCYTVDVDGQHVDMAAVSMGNP
HAVLRVDDINNAPVHELGPKIEHHPRFPARVNVGFLHVVDRQRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGHSVMMTGPAVRVYEGQVRL
>Mature_276_residues
MLLRFTKMHGLGNDFMVLDLVSQHAHILPKHAKQWGDRHTGIGFDQLLLVEAPNNPDVDFRYRIFNSDGSEVEQCGNGAR
CFARFVLDKRLTAKKQIRVETKSGIIELDIRSDGQISVDMGPPRFVPEEIPFEAAEQATCYTVDVDGQHVDMAAVSMGNP
HAVLRVDDINNAPVHELGPKIEHHPRFPARVNVGFLHVVDRQRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
LIDLPGGRLSIEWAGPGHSVMMTGPAVRVYEGQVRL

Specific function: Biosynthesis of lysine from aspartate semialdehyde; sixth step. [C]

COG id: COG0253

COG function: function code E; Diaminopimelate epimerase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the diaminopimelate epimerase family [H]

Homologues:

Organism=Escherichia coli, GI87082334, Length=274, Percent_Identity=54.3795620437956, Blast_Score=319, Evalue=1e-88,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001653
- InterPro:   IPR018510 [H]

Pfam domain/function: PF01678 DAP_epimerase [H]

EC number: =5.1.1.7 [H]

Molecular weight: Translated: 30399; Mature: 30399

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS01326 DAP_EPIMERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLLRFTKMHGLGNDFMVLDLVSQHAHILPKHAKQWGDRHTGIGFDQLLLVEAPNNPDVDF
CEEEEEECCCCCCCEEEEEEHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCEE
RYRIFNSDGSEVEQCGNGARCFARFVLDKRLTAKKQIRVETKSGIIELDIRSDGQISVDM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCEEEEEC
GPPRFVPEEIPFEAAEQATCYTVDVDGQHVDMAAVSMGNPHAVLRVDDINNAPVHELGPK
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHCCC
IEHHPRFPARVNVGFLHVVDRQRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
CCCCCCCCCEECCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
LIDLPGGRLSIEWAGPGHSVMMTGPAVRVYEGQVRL
EEECCCCEEEEEECCCCCEEEEECCEEEEECCEECC
>Mature Secondary Structure
MLLRFTKMHGLGNDFMVLDLVSQHAHILPKHAKQWGDRHTGIGFDQLLLVEAPNNPDVDF
CEEEEEECCCCCCCEEEEEEHHHHHHHCCHHHHHCCCCCCCCCCCEEEEEECCCCCCCEE
RYRIFNSDGSEVEQCGNGARCFARFVLDKRLTAKKQIRVETKSGIIELDIRSDGQISVDM
EEEEECCCCHHHHHHCCHHHHHHHHHHHHHCCCCCEEEEEECCCEEEEEECCCCEEEEEC
GPPRFVPEEIPFEAAEQATCYTVDVDGQHVDMAAVSMGNPHAVLRVDDINNAPVHELGPK
CCCCCCCCCCCCCCCCCCEEEEEECCCCEEEEEEEECCCCEEEEEEECCCCCCHHHHCCC
IEHHPRFPARVNVGFLHVVDRQRAQLRVWERGAGETQACGTGACAAAVAAISQGWMDSPL
CCCCCCCCCEECCEEEEEECCCCCEEEEEECCCCCCCCCCCHHHHHHHHHHHCCCCCCCE
LIDLPGGRLSIEWAGPGHSVMMTGPAVRVYEGQVRL
EEECCCCEEEEEECCCCCEEEEECCEEEEECCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA