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The map label for this gene is sbp [H]

Identifier: 66043355

GI number: 66043355

Start: 90266

End: 91276

Strand: Reverse

Name: sbp [H]

Synonym: Psyr_0084

Alternate gene names: 66043355

Gene position: 91276-90266 (Counterclockwise)

Preceding gene: 66043356

Following gene: 66043354

Centisome position: 1.5

GC content: 59.05

Gene sequence:

>1011_bases
ATGTCCATTCGCCGTTTTGCGCTGGCGGCTCTCGCCAGTGCTGTTTTCGCAGGTTCGGCTGTCGCCAAGGATTACGAACT
GCTCAACGTGTCCTACGATCCGACCCGCGAGCTGTATCAGGACTACAACGCAGAATTCGTCAATTTCTGGAAAAAATCCC
ACCCGGACGACAAGGTCGAGATCAAGCAATCTCACGGCGGCTCGGGCAAGCAGGCGCGTGGCGTGATCGACGGCCTGCGT
GCCGACGTGGTGACCCTGGCACTGGCCGGTGACATCGACGAAATCGCCAGGCTCGGCAAGACCCTGCCGGAAAACTGGCA
GACCCGCCTGCCGGACGCGAGCACACCGTACACCTCGACCATCGTGTTTCTGGTGCGCAAGGGCAACCCGAAAGGCATCA
AGGACTGGGGCGACCTGACCAAGGAAGGCGTCTCGGTGATCACCCCCAACCCGAAAACCTCGGGCGGTGCGCGCTGGAAC
TTCCTGGCCGCCTGGGCCTACGGCCTGAAGGCGGGCGGCGGTGACGAAGCCAAGGCCAAGGAATACGTACAGACGCTGTT
CAAGCACGTGCCGATCCTGGACACCGGCGCGCGCGGTTCGACCATCACCTTTGTCAACAACGGTCAGGGTGACGTGCTGC
TGGCGTGGGAAAACGAAGCGTTTCTGGCGTTGAAAGAAGACGGCGGCAAGGACAAGTTCGACATCGTCGTACCGTCGCTG
TCGATCCTTGCCGAACCTCCGGTCGCCGTGGTCGACAAGAACGCCGAGCGGAAAGGCAACAGCGAAATCGCGACTGAATA
CCTCAAGCACCTGTACAGCAAGGAAGGTCAGGAAATCGCCGCGAAAAACTTCTATCGTCCACGTGATGCCGAAGTGGCCG
CCAAGTACGAGAAGCAATTCCCGAAACTCGATCTGGTGACTATCGACAAAGACTTTGGTGGCTGGAAAACTGCGCAGCCG
AAGTTTTTCAATGACGGCGGCGTGTTCGATCAGATTTATCAGGCGCAATAA

Upstream 100 bases:

>100_bases
ACCCCCTGAAAAAAAGCAGCGCGCCCGTGCGGATGCCGTTCTGACGCGCCAAGAAGAAATCAAAAAAGCCAACACACCGT
ATTTCCAGGAGCTTCAATCT

Downstream 100 bases:

>100_bases
GCCGAATGAGGATGCCGCACCAGCGGCATCACAGGTTTGGCCAAACAGCCCGGATTAACCTCCGGGCTTCGTGCAAGTGA
AACCAGGGACTTTTATGTCG

Product: extracellular solute-binding protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: Sulfate starvation-induced protein 2; SSI2 [H]

Number of amino acids: Translated: 336; Mature: 335

Protein sequence:

>336_residues
MSIRRFALAALASAVFAGSAVAKDYELLNVSYDPTRELYQDYNAEFVNFWKKSHPDDKVEIKQSHGGSGKQARGVIDGLR
ADVVTLALAGDIDEIARLGKTLPENWQTRLPDASTPYTSTIVFLVRKGNPKGIKDWGDLTKEGVSVITPNPKTSGGARWN
FLAAWAYGLKAGGGDEAKAKEYVQTLFKHVPILDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGKDKFDIVVPSL
SILAEPPVAVVDKNAERKGNSEIATEYLKHLYSKEGQEIAAKNFYRPRDAEVAAKYEKQFPKLDLVTIDKDFGGWKTAQP
KFFNDGGVFDQIYQAQ

Sequences:

>Translated_336_residues
MSIRRFALAALASAVFAGSAVAKDYELLNVSYDPTRELYQDYNAEFVNFWKKSHPDDKVEIKQSHGGSGKQARGVIDGLR
ADVVTLALAGDIDEIARLGKTLPENWQTRLPDASTPYTSTIVFLVRKGNPKGIKDWGDLTKEGVSVITPNPKTSGGARWN
FLAAWAYGLKAGGGDEAKAKEYVQTLFKHVPILDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGKDKFDIVVPSL
SILAEPPVAVVDKNAERKGNSEIATEYLKHLYSKEGQEIAAKNFYRPRDAEVAAKYEKQFPKLDLVTIDKDFGGWKTAQP
KFFNDGGVFDQIYQAQ
>Mature_335_residues
SIRRFALAALASAVFAGSAVAKDYELLNVSYDPTRELYQDYNAEFVNFWKKSHPDDKVEIKQSHGGSGKQARGVIDGLRA
DVVTLALAGDIDEIARLGKTLPENWQTRLPDASTPYTSTIVFLVRKGNPKGIKDWGDLTKEGVSVITPNPKTSGGARWNF
LAAWAYGLKAGGGDEAKAKEYVQTLFKHVPILDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGKDKFDIVVPSLS
ILAEPPVAVVDKNAERKGNSEIATEYLKHLYSKEGQEIAAKNFYRPRDAEVAAKYEKQFPKLDLVTIDKDFGGWKTAQPK
FFNDGGVFDQIYQAQ

Specific function: This protein specifically binds sulfate and is involved in its transmembrane transport [H]

COG id: COG1613

COG function: function code P; ABC-type sulfate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic sulfate-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI1790351, Length=316, Percent_Identity=66.4556962025316, Blast_Score=415, Evalue=1e-117,
Organism=Escherichia coli, GI1788765, Length=310, Percent_Identity=44.5161290322581, Blast_Score=270, Evalue=8e-74,

Paralogues:

None

Copy number: 1240 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR000957
- InterPro:   IPR005669 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 36940; Mature: 36809

Theoretical pI: Translated: 6.55; Mature: 6.55

Prosite motif: PS00757 PROK_SULFATE_BIND_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.3 %Met     (Translated Protein)
0.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIRRFALAALASAVFAGSAVAKDYELLNVSYDPTRELYQDYNAEFVNFWKKSHPDDKVE
CCHHHHHHHHHHHHHHHCHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCEE
IKQSHGGSGKQARGVIDGLRADVVTLALAGDIDEIARLGKTLPENWQTRLPDASTPYTST
EEECCCCCCCHHHHHHHHHHHHHEEHHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCEE
IVFLVRKGNPKGIKDWGDLTKEGVSVITPNPKTSGGARWNFLAAWAYGLKAGGGDEAKAK
EEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
EYVQTLFKHVPILDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGKDKFDIVVPSL
HHHHHHHHHCCCEECCCCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCEEEEEECCH
SILAEPPVAVVDKNAERKGNSEIATEYLKHLYSKEGQEIAAKNFYRPRDAEVAAKYEKQF
HHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCC
PKLDLVTIDKDFGGWKTAQPKFFNDGGVFDQIYQAQ
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCC
>Mature Secondary Structure 
SIRRFALAALASAVFAGSAVAKDYELLNVSYDPTRELYQDYNAEFVNFWKKSHPDDKVE
CHHHHHHHHHHHHHHHCHHHHCCEEEEECCCCCHHHHHHHCCCHHHHHHHHCCCCCCEE
IKQSHGGSGKQARGVIDGLRADVVTLALAGDIDEIARLGKTLPENWQTRLPDASTPYTST
EEECCCCCCCHHHHHHHHHHHHHEEHHHHCCHHHHHHHHCCCCCCHHHCCCCCCCCCCEE
IVFLVRKGNPKGIKDWGDLTKEGVSVITPNPKTSGGARWNFLAAWAYGLKAGGGDEAKAK
EEEEEECCCCCCCCHHHHHHHCCCEEECCCCCCCCCCCCHHHHHHHHHHCCCCCCHHHHH
EYVQTLFKHVPILDTGARGSTITFVNNGQGDVLLAWENEAFLALKEDGGKDKFDIVVPSL
HHHHHHHHHCCCEECCCCCCEEEEEECCCCCEEEEECCCEEEEEECCCCCCEEEEEECCH
SILAEPPVAVVDKNAERKGNSEIATEYLKHLYSKEGQEIAAKNFYRPRDAEVAAKYEKQF
HHHCCCCCEEECCCCCCCCCHHHHHHHHHHHHCCCCCHHHHHCCCCCCCHHHHHHHHHCC
PKLDLVTIDKDFGGWKTAQPKFFNDGGVFDQIYQAQ
CCEEEEEEECCCCCCCCCCCCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3158524; 2002055; 8346018; 9278503; 9298646; 8774726 [H]