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The map label for this gene is cysU [H]

Identifier: 66043354

GI number: 66043354

Start: 89350

End: 90171

Strand: Reverse

Name: cysU [H]

Synonym: Psyr_0083

Alternate gene names: 66043354

Gene position: 90171-89350 (Counterclockwise)

Preceding gene: 66043355

Following gene: 66043353

Centisome position: 1.48

GC content: 63.5

Gene sequence:

>822_bases
ATGTCGCGTCGTATCTCCCCCGTCATACCCGGCTTCGGGCTGACGCTGGGCTACACCTTGGTGTACCTCAGTCTGATTGT
GCTAATACCGCTGGCCGCCATGTTCGTACATGCCGCGCAGCTCACCTGGGATCAGTTCTGGACCATCATCACGGCCCCTC
GCGTGATCGCGGCACTCAAGCTGAGTTTCGGCACCGCCTTTTACGCAGCCCTCATCAACGGGGTGATCGGCACACTGCTG
GCCTGGGTGCTGGTGCGCTACACCTTCCCCGGGCGCAAGGTCATCGATGCGATGATCGATCTGCCATTCGCCCTGCCGAC
CGCCGTGGCCGGTATCGCCCTGACCGCGCTGTACGCGCCCAACGGCTGGGTCGGCCAGTTCGCGGCTGATCTGGGTTTCA
AGATCGCCTACACCCCGTTGGGCATCACCCTGGCGCTGACCTTCGTCACCCTGCCGTTCGTGGTGCGCACCGTGCAGCCT
GTGCTGGCCGACATCCCCCGCGAAGTCGAAGAGGCCGCCGCCTGCCTGGGCGCGCGTCCGCTACAGGTGTTTCGCCACAT
CCTCGTCCCGGCGTTGCTGCCTGCCTGGCTGACCGGCTTCGCCCTGGCGTTTGCCCGTGGCGTGGGCGAGTACGGTTCGG
TGATTTTCATCGCCGGCAACATGCCGATGAAAACCGAGATCCTGCCGCTGCTGATCATGGTCAAGCTCGACCAGTACGAC
TACACCGGCGCAACCGCTATCGGCGTGATGATGCTGGTGGTGTCCTTCATTCTGCTGCTGCTGATCAACCTGCTGCAGCG
GCGCATTGAAACCCCATCCTGA

Upstream 100 bases:

>100_bases
CAATAAGCCGAATGAGGATGCCGCACCAGCGGCATCACAGGTTTGGCCAAACAGCCCGGATTAACCTCCGGGCTTCGTGC
AAGTGAAACCAGGGACTTTT

Downstream 100 bases:

>100_bases
GGAGGCGCAGAACATGTCTTATTCATCTGTCTCGGCCGCCGCCTCTGCCAACGCCGCCCGTCGTGGCAGCGCGGTATCGC
GACGCATCCTGATCGGCCTG

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIITAPRVIAALKLSFGTAFYAALINGVIGTLL
AWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYAPNGWVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQP
VLADIPREVEEAAACLGARPLQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
YTGATAIGVMMLVVSFILLLLINLLQRRIETPS

Sequences:

>Translated_273_residues
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIITAPRVIAALKLSFGTAFYAALINGVIGTLL
AWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYAPNGWVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQP
VLADIPREVEEAAACLGARPLQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
YTGATAIGVMMLVVSFILLLLINLLQRRIETPS
>Mature_272_residues
SRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIITAPRVIAALKLSFGTAFYAALINGVIGTLLA
WVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYAPNGWVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPV
LADIPREVEEAAACLGARPLQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYDY
TGATAIGVMMLVVSFILLLLINLLQRRIETPS

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG0555

COG function: function code O; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1788764, Length=267, Percent_Identity=50.561797752809, Blast_Score=247, Evalue=7e-67,
Organism=Escherichia coli, GI87082099, Length=267, Percent_Identity=31.8352059925094, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI1786980, Length=222, Percent_Identity=33.7837837837838, Blast_Score=91, Evalue=1e-19,
Organism=Escherichia coli, GI1786254, Length=274, Percent_Identity=28.1021897810219, Blast_Score=78, Evalue=5e-16,
Organism=Escherichia coli, GI1787368, Length=232, Percent_Identity=27.1551724137931, Blast_Score=61, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011865
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 29604; Mature: 29473

Theoretical pI: Translated: 9.53; Mature: 9.53

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIITAPRVIAALK
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFGTAFYAALINGVIGTLLAWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
NGWVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPVLADIPREVEEAAACLGARP
CCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
LQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
YTGATAIGVMMLVVSFILLLLINLLQRRIETPS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SRRISPVIPGFGLTLGYTLVYLSLIVLIPLAAMFVHAAQLTWDQFWTIITAPRVIAALK
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LSFGTAFYAALINGVIGTLLAWVLVRYTFPGRKVIDAMIDLPFALPTAVAGIALTALYAP
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
NGWVGQFAADLGFKIAYTPLGITLALTFVTLPFVVRTVQPVLADIPREVEEAAACLGARP
CCCHHHHHHHCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHCCCH
LQVFRHILVPALLPAWLTGFALAFARGVGEYGSVIFIAGNMPMKTEILPLLIMVKLDQYD
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCHHHHHHHHHHHHCCCCC
YTGATAIGVMMLVVSFILLLLINLLQRRIETPS
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 2188958; 9205837; 9278503; 2188959 [H]