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The map label for this gene is cysW [H]

Identifier: 66043353

GI number: 66043353

Start: 88464

End: 89336

Strand: Reverse

Name: cysW [H]

Synonym: Psyr_0082

Alternate gene names: 66043353

Gene position: 89336-88464 (Counterclockwise)

Preceding gene: 66043354

Following gene: 66043352

Centisome position: 1.47

GC content: 61.86

Gene sequence:

>873_bases
ATGTCTTATTCATCTGTCTCGGCCGCCGCCTCTGCCAACGCCGCCCGTCGTGGCAGCGCGGTATCGCGACGCATCCTGAT
CGGCCTGTGCTGGCTGGTATTCGTCCTGTTTCTGGGCCTGCCGCTGTTCATCGTGGTGTCCCAGGGCCTGAAAAACGGTC
TGGGCGCGTTCTTTATCGCAATCCTCGAACCGGACGCGCTGTCGGCGCTGAAGCTGACGGTCATAGCGGTATTGATCTCG
GTGCCGCTAAACCTGGTGTTCGGCGTCAGCGCCGCGTGGTGTGTGAGCAAATACTCGTTCCGCGGCAAGAGCATTCTGGT
CACGCTGATCGACCTGCCGTTCTCGGTGTCGCCGGTCATCGCCGGTCTGGTCTACGTGCTGATGTTCGGTGCACAGGGGC
TGTTCGGGCCGTGGCTGCAGGATCACGACATCCAGATCGTCTTTGCCCTGCCGGGCATCGTGCTGGCGACCATCTTCGTC
ACCGTCCCCTTCGTTGCCCGTGAGCTGATCCCGCTGATGCAGGAGCAGGGCACTCAGGAAGAAGAAGCCGCACGGCTGCT
GGGCGCCAATGGCTGGCAGATGTTCTGGCACGTCACGGTGCCGAACATCAAATGGGGCCTGATCTACGGCGTGGTGCTGT
GTACGGCGCGGGCGATGGGTGAGTTTGGCGCGGTGTCGGTGGTGTCCGGGCACATTCGCGGCGTCACCAACACCCTGCCG
CTGCACGTCGAAATCCTCTACAACGAATACAACCACGTTGCCGCCTTTGCCGTGGCGAGCCTGTTGCTGATCCTGGCGCT
GTTCATCCTGCTGCTCAAGCAGTGGAGCGAATCGCGCATTAACCGTCTGCGCCATAACGCGGCGGAGGAATAA

Upstream 100 bases:

>100_bases
GCTATCGGCGTGATGATGCTGGTGGTGTCCTTCATTCTGCTGCTGCTGATCAACCTGCTGCAGCGGCGCATTGAAACCCC
ATCCTGAGGAGGCGCAGAAC

Downstream 100 bases:

>100_bases
TCATGTCGATCGAAGTCCGTAACGTCAGCAAGAACTTCAATGCCTTCAAGGCCCTGAACAACATCAGTCTGGACATCCAG
AGTGGCGAGCTGGTGGCTTT

Product: binding-protein dependent transport system inner membrane protein

Products: ADP; phosphate; S2O32- [Cytoplasm]; SO42- [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MSYSSVSAAASANAARRGSAVSRRILIGLCWLVFVLFLGLPLFIVVSQGLKNGLGAFFIAILEPDALSALKLTVIAVLIS
VPLNLVFGVSAAWCVSKYSFRGKSILVTLIDLPFSVSPVIAGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFV
TVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSESRINRLRHNAAEE

Sequences:

>Translated_290_residues
MSYSSVSAAASANAARRGSAVSRRILIGLCWLVFVLFLGLPLFIVVSQGLKNGLGAFFIAILEPDALSALKLTVIAVLIS
VPLNLVFGVSAAWCVSKYSFRGKSILVTLIDLPFSVSPVIAGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFV
TVPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSESRINRLRHNAAEE
>Mature_289_residues
SYSSVSAAASANAARRGSAVSRRILIGLCWLVFVLFLGLPLFIVVSQGLKNGLGAFFIAILEPDALSALKLTVIAVLISV
PLNLVFGVSAAWCVSKYSFRGKSILVTLIDLPFSVSPVIAGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFVT
VPFVARELIPLMQEQGTQEEEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLPL
HVEILYNEYNHVAAFAVASLLLILALFILLLKQWSESRINRLRHNAAEE

Specific function: Part of the ABC transporter complex CysAWTP (TC 3.A.1.6.1) involved in sulfate/thiosulfate import. Probably responsible for the translocation of the substrate across the membrane [H]

COG id: COG4208

COG function: function code P; ABC-type sulfate transport system, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI87082099, Length=265, Percent_Identity=52.0754716981132, Blast_Score=278, Evalue=3e-76,
Organism=Escherichia coli, GI1788764, Length=222, Percent_Identity=36.036036036036, Blast_Score=151, Evalue=4e-38,
Organism=Escherichia coli, GI1786980, Length=222, Percent_Identity=33.3333333333333, Blast_Score=93, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515
- InterPro:   IPR011866
- InterPro:   IPR005667 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 31502; Mature: 31371

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00656 GLYCOSYL_HYDROL_F6_2 ; PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYSSVSAAASANAARRGSAVSRRILIGLCWLVFVLFLGLPLFIVVSQGLKNGLGAFFIA
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHE
ILEPDALSALKLTVIAVLISVPLNLVFGVSAAWCVSKYSFRGKSILVTLIDLPFSVSPVI
EECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
AGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFVTVPFVARELIPLMQEQGTQE
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
HHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSESRINRLRHNAAEE
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SYSSVSAAASANAARRGSAVSRRILIGLCWLVFVLFLGLPLFIVVSQGLKNGLGAFFIA
CCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHE
ILEPDALSALKLTVIAVLISVPLNLVFGVSAAWCVSKYSFRGKSILVTLIDLPFSVSPVI
EECCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHH
AGLVYVLMFGAQGLFGPWLQDHDIQIVFALPGIVLATIFVTVPFVARELIPLMQEQGTQE
HHHHHHHHHCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
EEAARLLGANGWQMFWHVTVPNIKWGLIYGVVLCTARAMGEFGAVSVVSGHIRGVTNTLP
HHHHHHHCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCC
LHVEILYNEYNHVAAFAVASLLLILALFILLLKQWSESRINRLRHNAAEE
EEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; S2O32- [Periplasm]; H2O; SO42- [Periplasm]; ATP [C]

Specific reaction: ATP + S2O32- [Periplasm] + H2O = ADP + phosphate + S2O32- [Cytoplasm] SO42- [Periplasm] + H2O + ATP = SO42- [Cytoplasm] + phosphate + ADP [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]