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The map label for this gene is blh [H]

Identifier: 66043349

GI number: 66043349

Start: 84310

End: 85194

Strand: Direct

Name: blh [H]

Synonym: Psyr_0078

Alternate gene names: 66043349

Gene position: 84310-85194 (Clockwise)

Preceding gene: 66043347

Following gene: 66043350

Centisome position: 1.38

GC content: 61.36

Gene sequence:

>885_bases
ATGATTATCGGTGAAAAGCTTCATGTCGAAGCGCTCTTCGACACGCATACCTGGACCATCAGCTATCTGGTGATGGACCT
GCACAGCAAGCAATGTGCGCTGATCGACAGCGTGCTCGATTACGACCCCAAGTCCGGCCGCACCAGAACCGAGTCGGCCG
ACCGGATGATCGAACGGGCGCAGGCCCTGGGCGCCTCGGTGCAGTGGATTTTCGAAACCCATGTGCACGCCGATCACCTC
TCGGCAGCGCCGTATCTGAAACAGCGGCTCGGTGGGCAAATTGTCATCGGCAGCCATATCACTGCGGTGCAGGAAACCTT
CGGCACGCTGTTCAACGCGCCGCCTGACTTTGCCCGCAATGGCAGTCAGTTCGATGTGCTGCTGGAAGACAACGCCTCGT
TCGCCCTCGGCGCGCTTGAGGTCAAGGCCATGCACACGCCCGGGCACACGCCGGCGTGCATGAGTTACCTGGTGCAAGTC
GGTGACGCCACGGTCGCGTTTGTCGGCGATACGCTGTTCATGCCCGATTACGGCACCGCTCGGTGCGACTTCCCGGGTGC
CGATGCGCGCACGCTGTATCGCTCGATCAAGAAGCTGCTGGCGCTGCCGCCGGAAACCCTGCTGTTCATGTGCCACGACT
ACCTGCCCAATGGCCGTGAGCTGAAATACATGACCACGGTTGCCGAACAGCGTGCCAGCAATATCCATGTGCACGACGGG
GTGGATGAAGACGCGTTCGTCGGCATGCGCGAAGCCCGTGACGCGACGCTGGACATGCCGGTGCTGATGCTGCCTGCGGT
GCAGGTCAACATGCGCTGCGGCCACTTTCCCGAGCCGGAAGACAACGGCGTCGTCTACCTGAAGATCCCGCTCAATGCCC
TTTGA

Upstream 100 bases:

>100_bases
CAGTGATTGGCATGGTTATTGAGACATTATGGCAACGACTGCCAACCGTCCGGCCTGGCCAAACGGGCCTTGAGGCGATT
TACAGCCACGGGAATTGCAC

Downstream 100 bases:

>100_bases
CCGCTGCCCGCCTTGGGAAACCCTGTGATGAATGCTTCGACAACAACCTGTCAGATCCTGATCGTCGGTGCCGGTGCTGC
CGGTATCGCCACTGCCGCAA

Product: Beta-lactamase-like

Products: Reduced glutathione; H+; D-Lactate [C]

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MIIGEKLHVEALFDTHTWTISYLVMDLHSKQCALIDSVLDYDPKSGRTRTESADRMIERAQALGASVQWIFETHVHADHL
SAAPYLKQRLGGQIVIGSHITAVQETFGTLFNAPPDFARNGSQFDVLLEDNASFALGALEVKAMHTPGHTPACMSYLVQV
GDATVAFVGDTLFMPDYGTARCDFPGADARTLYRSIKKLLALPPETLLFMCHDYLPNGRELKYMTTVAEQRASNIHVHDG
VDEDAFVGMREARDATLDMPVLMLPAVQVNMRCGHFPEPEDNGVVYLKIPLNAL

Sequences:

>Translated_294_residues
MIIGEKLHVEALFDTHTWTISYLVMDLHSKQCALIDSVLDYDPKSGRTRTESADRMIERAQALGASVQWIFETHVHADHL
SAAPYLKQRLGGQIVIGSHITAVQETFGTLFNAPPDFARNGSQFDVLLEDNASFALGALEVKAMHTPGHTPACMSYLVQV
GDATVAFVGDTLFMPDYGTARCDFPGADARTLYRSIKKLLALPPETLLFMCHDYLPNGRELKYMTTVAEQRASNIHVHDG
VDEDAFVGMREARDATLDMPVLMLPAVQVNMRCGHFPEPEDNGVVYLKIPLNAL
>Mature_294_residues
MIIGEKLHVEALFDTHTWTISYLVMDLHSKQCALIDSVLDYDPKSGRTRTESADRMIERAQALGASVQWIFETHVHADHL
SAAPYLKQRLGGQIVIGSHITAVQETFGTLFNAPPDFARNGSQFDVLLEDNASFALGALEVKAMHTPGHTPACMSYLVQV
GDATVAFVGDTLFMPDYGTARCDFPGADARTLYRSIKKLLALPPETLLFMCHDYLPNGRELKYMTTVAEQRASNIHVHDG
VDEDAFVGMREARDATLDMPVLMLPAVQVNMRCGHFPEPEDNGVVYLKIPLNAL

Specific function: Thiolesterase That Catalyzes The Hydrolysis Of S-D- Lactoyl-Glutathione To Form Glutathione And D-Lactic Acid. [C]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-B beta-lactamase family [H]

Homologues:

Organism=Homo sapiens, GI41327741, Length=272, Percent_Identity=29.0441176470588, Blast_Score=102, Evalue=4e-22,
Organism=Drosophila melanogaster, GI221330176, Length=271, Percent_Identity=28.0442804428044, Blast_Score=102, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005939
- InterPro:   IPR001279 [H]

Pfam domain/function: PF04273 DUF442; PF00753 Lactamase_B [H]

EC number: 3.1.2.6 [C]

Molecular weight: Translated: 32519; Mature: 32519

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
4.1 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
4.1 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIIGEKLHVEALFDTHTWTISYLVMDLHSKQCALIDSVLDYDPKSGRTRTESADRMIERA
CCCCCEEEEEEEECCCCEEEEHHEEHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
QALGASVQWIFETHVHADHLSAAPYLKQRLGGQIVIGSHITAVQETFGTLFNAPPDFARN
HHHCCCEEEEEECCCCHHHHCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
GSQFDVLLEDNASFALGALEVKAMHTPGHTPACMSYLVQVGDATVAFVGDTLFMPDYGTA
CCEEEEEEECCCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCEEECCCCCCC
RCDFPGADARTLYRSIKKLLALPPETLLFMCHDYLPNGRELKYMTTVAEQRASNIHVHDG
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEECC
VDEDAFVGMREARDATLDMPVLMLPAVQVNMRCGHFPEPEDNGVVYLKIPLNAL
CCCHHHHCHHHHCCCCCCCCEEEECCEEEEEECCCCCCCCCCCEEEEEECCCCC
>Mature Secondary Structure
MIIGEKLHVEALFDTHTWTISYLVMDLHSKQCALIDSVLDYDPKSGRTRTESADRMIERA
CCCCCEEEEEEEECCCCEEEEHHEEHHCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHH
QALGASVQWIFETHVHADHLSAAPYLKQRLGGQIVIGSHITAVQETFGTLFNAPPDFARN
HHHCCCEEEEEECCCCHHHHCCCHHHHHHCCCEEEECCCHHHHHHHHHHHHCCCHHHHCC
GSQFDVLLEDNASFALGALEVKAMHTPGHTPACMSYLVQVGDATVAFVGDTLFMPDYGTA
CCEEEEEEECCCCEEEEEEEEEEEECCCCCHHHHHHHHHHCCCEEEEECCEEECCCCCCC
RCDFPGADARTLYRSIKKLLALPPETLLFMCHDYLPNGRELKYMTTVAEQRASNIHVHDG
CCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCEEEECC
VDEDAFVGMREARDATLDMPVLMLPAVQVNMRCGHFPEPEDNGVVYLKIPLNAL
CCCHHHHCHHHHCCCCCCCCEEEECCEEEEEECCCCCCCCCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; (R)-S-Lactoylglutathione [C]

Specific reaction: H2O + (R)-S-Lactoylglutathione --> Reduced glutathione + H+ + D-Lactate [C]

General reaction: Thioester hydrolysis [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 10910347 [H]