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The map label for this gene is rsmB [H]

Identifier: 66043288

GI number: 66043288

Start: 20699

End: 22045

Strand: Reverse

Name: rsmB [H]

Synonym: Psyr_0017

Alternate gene names: 66043288

Gene position: 22045-20699 (Counterclockwise)

Preceding gene: 66043289

Following gene: 66043287

Centisome position: 0.36

GC content: 65.33

Gene sequence:

>1347_bases
ATGAACCCGCGTCTGGCCGCCGCCAAGGCACTGGCTGCCGTACTCAGCGGCAAGGCCTCGCTGAACAGCTCGTTGCCGAC
TCAACTGGACAAGGTCGAGGTGCGTGATCGTGGCCTGACGCAGGACCTGGCATTCGGCACCGCGCGCTGGCAGCCGCGTC
TGTCGGCATTGGCGGCCAAGCTGCTGCAGAAGCCCTTCAAGGCCGCCGACGCCGACGTTGAAGCCTTGTTGCTGGTCGGC
CTTTATCAACTGTTCTACAGCCGCATTCCGGCCCATGCTGCCATTGGCGAAACCGTGGGCTGCGCCGACAAATTGAAAAA
GCCGTGGGCCAAAGGCTTGCTCAACGCCGTGCTGCGCAATGCGCAACGTGACGGCGAGGCGCTGCTGATCGAGCTGGAAC
ACGACCCGGTGGTGCGTACCGCCCACCCGCGCTGGCTGCAGAAAGCTTTGAAAGCCGCCTGGCCGGAACAGTGGGAAGCC
ATCTGCGCGGCCAACAATGCGCACCCGCCAATGATCTTGCGCGTCAACCGACGCCATCACCGTCGCGATCAATACCTCGA
ACTGCTCGACACTGCCGGCCTGCAAGCCAGTGCGTGCACGTTCAGTCAGGACGGCATCGTGCTCGCCGAGCCGTGCGATG
TGCGCTCGCTGCCCGGTTTTGCCGAAGGCTGGATCAGCGTGCAGGATGAAGCCGCGCAGCTGGCCGCCGACCTGCTGGAA
CTGGCCCCCGGGCAACGGGTACTCGATGCCTGCTGCGCGCCGGGCGGCAAGACCTGCCATTTGCTCGAAGTACAGCCGCA
ACTGGACGGCGTGGTGGCGGTGGATCTGGAAGCCAAGCGTCTGGTGCGCGTGCGTGAAAACCTCGATCGCCTGGGGCTGG
ATGCCGAGCTGATTGCCGCCGACGCCCGCGAGACCGCGCAGTGGTGGGACGGCAAACCGTTCCAGCGCATCCTGCTCGAC
GCACCGTGCTCGGCGACCGGCGTGATTCGTCGGCATCCGGACATCAAACTGACTCGTCAGGCAGACGACATCGCCGCGCT
GGCGGCCTTGCAGGGCGAGCTGCTCGATGCGTTGTGGCCTACGCTGCAGGTAGGCGGCATGCTGGTGTACGCGACCTGCT
CGACACTGCCAACCGAAAACACCGACGTCATCGAGGCGTTTCTGGCGCGTACCTCCGGGGCCAGAGAGCTGGATATCGCC
GGGCAGGCCGGACAGCCGCCCGCGGGTATCAAGCAAGCCCATGGTCGCCAGCTGCTGGCCCAGCAAGGCGGCCACGACGG
TTTCTACTACGCCAAACTGATCAAGATCGCGGATCAAGATCGCGGCGGCGCGGGAGTAAGCGCGTGA

Upstream 100 bases:

>100_bases
GCCTGACCCGTCTGCAATTGCCGGGCGGCAAGCCGCTGAACTTCACTGACCTGTTCAACAGCCGCCGCGAGAAATTTGCC
ATCGGCACGGTGCTGGGCCA

Downstream 100 bases:

>100_bases
AAATCATCATTCTGGGTGCAGGTCAGGTAGGCGGTACGCTGGCCGAGCATCTGGCGGGCGAAGCCAACGACATCACCGTG
GTCGACACTGACGGCGACCG

Product: Fmu, rRNA SAM-dependent methyltransferase

Products: NA

Alternate protein names: 16S rRNA m5C967 methyltransferase; rRNA (cytosine-C(5)-)-methyltransferase rsmB [H]

Number of amino acids: Translated: 448; Mature: 448

Protein sequence:

>448_residues
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEVRDRGLTQDLAFGTARWQPRLSALAAKLLQKPFKAADADVEALLLVG
LYQLFYSRIPAHAAIGETVGCADKLKKPWAKGLLNAVLRNAQRDGEALLIELEHDPVVRTAHPRWLQKALKAAWPEQWEA
ICAANNAHPPMILRVNRRHHRRDQYLELLDTAGLQASACTFSQDGIVLAEPCDVRSLPGFAEGWISVQDEAAQLAADLLE
LAPGQRVLDACCAPGGKTCHLLEVQPQLDGVVAVDLEAKRLVRVRENLDRLGLDAELIAADARETAQWWDGKPFQRILLD
APCSATGVIRRHPDIKLTRQADDIAALAALQGELLDALWPTLQVGGMLVYATCSTLPTENTDVIEAFLARTSGARELDIA
GQAGQPPAGIKQAHGRQLLAQQGGHDGFYYAKLIKIADQDRGGAGVSA

Sequences:

>Translated_448_residues
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEVRDRGLTQDLAFGTARWQPRLSALAAKLLQKPFKAADADVEALLLVG
LYQLFYSRIPAHAAIGETVGCADKLKKPWAKGLLNAVLRNAQRDGEALLIELEHDPVVRTAHPRWLQKALKAAWPEQWEA
ICAANNAHPPMILRVNRRHHRRDQYLELLDTAGLQASACTFSQDGIVLAEPCDVRSLPGFAEGWISVQDEAAQLAADLLE
LAPGQRVLDACCAPGGKTCHLLEVQPQLDGVVAVDLEAKRLVRVRENLDRLGLDAELIAADARETAQWWDGKPFQRILLD
APCSATGVIRRHPDIKLTRQADDIAALAALQGELLDALWPTLQVGGMLVYATCSTLPTENTDVIEAFLARTSGARELDIA
GQAGQPPAGIKQAHGRQLLAQQGGHDGFYYAKLIKIADQDRGGAGVSA
>Mature_448_residues
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEVRDRGLTQDLAFGTARWQPRLSALAAKLLQKPFKAADADVEALLLVG
LYQLFYSRIPAHAAIGETVGCADKLKKPWAKGLLNAVLRNAQRDGEALLIELEHDPVVRTAHPRWLQKALKAAWPEQWEA
ICAANNAHPPMILRVNRRHHRRDQYLELLDTAGLQASACTFSQDGIVLAEPCDVRSLPGFAEGWISVQDEAAQLAADLLE
LAPGQRVLDACCAPGGKTCHLLEVQPQLDGVVAVDLEAKRLVRVRENLDRLGLDAELIAADARETAQWWDGKPFQRILLD
APCSATGVIRRHPDIKLTRQADDIAALAALQGELLDALWPTLQVGGMLVYATCSTLPTENTDVIEAFLARTSGARELDIA
GQAGQPPAGIKQAHGRQLLAQQGGHDGFYYAKLIKIADQDRGGAGVSA

Specific function: Specifically methylates the cytosine at position 967 (m5C967) of 16S rRNA [H]

COG id: COG0144

COG function: function code J; tRNA and rRNA cytosine-C5-methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. RsmB/NOP family [H]

Homologues:

Organism=Homo sapiens, GI76150625, Length=338, Percent_Identity=30.7692307692308, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI76150623, Length=338, Percent_Identity=30.7692307692308, Blast_Score=123, Evalue=3e-28,
Organism=Homo sapiens, GI32698918, Length=193, Percent_Identity=29.5336787564767, Blast_Score=86, Evalue=6e-17,
Organism=Homo sapiens, GI270288816, Length=198, Percent_Identity=34.8484848484849, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI8922322, Length=198, Percent_Identity=34.8484848484849, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI23199998, Length=198, Percent_Identity=34.8484848484849, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI270288818, Length=198, Percent_Identity=34.8484848484849, Blast_Score=82, Evalue=1e-15,
Organism=Homo sapiens, GI301336155, Length=201, Percent_Identity=29.8507462686567, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI39995082, Length=201, Percent_Identity=29.8507462686567, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI11545785, Length=181, Percent_Identity=32.5966850828729, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI2367212, Length=434, Percent_Identity=49.3087557603687, Blast_Score=383, Evalue=1e-107,
Organism=Escherichia coli, GI87081985, Length=225, Percent_Identity=28.8888888888889, Blast_Score=77, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI17536757, Length=280, Percent_Identity=30, Blast_Score=108, Evalue=6e-24,
Organism=Caenorhabditis elegans, GI115534021, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI25143471, Length=186, Percent_Identity=30.1075268817204, Blast_Score=67, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI71998419, Length=190, Percent_Identity=29.4736842105263, Blast_Score=67, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6324268, Length=310, Percent_Identity=28.0645161290323, Blast_Score=102, Evalue=8e-23,
Organism=Saccharomyces cerevisiae, GI6319447, Length=182, Percent_Identity=32.967032967033, Blast_Score=74, Evalue=5e-14,
Organism=Drosophila melanogaster, GI22024126, Length=257, Percent_Identity=29.5719844357977, Blast_Score=97, Evalue=2e-20,
Organism=Drosophila melanogaster, GI21355201, Length=199, Percent_Identity=31.6582914572864, Blast_Score=85, Evalue=1e-16,
Organism=Drosophila melanogaster, GI24668781, Length=235, Percent_Identity=30.2127659574468, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI19920494, Length=177, Percent_Identity=31.638418079096, Blast_Score=80, Evalue=2e-15,
Organism=Drosophila melanogaster, GI21356513, Length=235, Percent_Identity=30.2127659574468, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001678
- InterPro:   IPR018314
- InterPro:   IPR006027
- InterPro:   IPR004573 [H]

Pfam domain/function: PF01189 Nol1_Nop2_Fmu; PF01029 NusB [H]

EC number: =2.1.1.176 [H]

Molecular weight: Translated: 48391; Mature: 48391

Theoretical pI: Translated: 6.67; Mature: 6.67

Prosite motif: PS01153 NOL1_NOP2_SUN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.0 %Cys     (Translated Protein)
0.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
2.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEVRDRGLTQDLAFGTARWQPRLSALAAK
CCCHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHH
LLQKPFKAADADVEALLLVGLYQLFYSRIPAHAAIGETVGCADKLKKPWAKGLLNAVLRN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
AQRDGEALLIELEHDPVVRTAHPRWLQKALKAAWPEQWEAICAANNAHPPMILRVNRRHH
CCCCCCEEEEEECCCCCEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCHHH
RRDQYLELLDTAGLQASACTFSQDGIVLAEPCDVRSLPGFAEGWISVQDEAAQLAADLLE
HHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCCCHHHCCEECHHHHHHHHHHHHH
LAPGQRVLDACCAPGGKTCHLLEVQPQLDGVVAVDLEAKRLVRVRENLDRLGLDAELIAA
HCCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHH
DARETAQWWDGKPFQRILLDAPCSATGVIRRHPDIKLTRQADDIAALAALQGELLDALWP
CHHHHHHHCCCCCHHHHHCCCCCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHH
TLQVGGMLVYATCSTLPTENTDVIEAFLARTSGARELDIAGQAGQPPAGIKQAHGRQLLA
HHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
QQGGHDGFYYAKLIKIADQDRGGAGVSA
HCCCCCCHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MNPRLAAAKALAAVLSGKASLNSSLPTQLDKVEVRDRGLTQDLAFGTARWQPRLSALAAK
CCCHHHHHHHHHHHHHCCHHCCCCCCCHHHHHHHHHCCCCHHHHCCCCCCCHHHHHHHHH
LLQKPFKAADADVEALLLVGLYQLFYSRIPAHAAIGETVGCADKLKKPWAKGLLNAVLRN
HHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCHHHHCCHHCCHHHHHHHHHHHHHHHHHHH
AQRDGEALLIELEHDPVVRTAHPRWLQKALKAAWPEQWEAICAANNAHPPMILRVNRRHH
CCCCCCEEEEEECCCCCEECCCHHHHHHHHHHCCHHHHHHHHHCCCCCCCEEEEECCHHH
RRDQYLELLDTAGLQASACTFSQDGIVLAEPCDVRSLPGFAEGWISVQDEAAQLAADLLE
HHHHHHHHHHHCCCCCCCEEECCCCEEEECCCCCCCCCCHHHCCEECHHHHHHHHHHHHH
LAPGQRVLDACCAPGGKTCHLLEVQPQLDGVVAVDLEAKRLVRVRENLDRLGLDAELIAA
HCCCHHHHHHHCCCCCCEEEEEEECCCCCCEEEEECHHHHHHHHHHHHHHHCCCHHHHHH
DARETAQWWDGKPFQRILLDAPCSATGVIRRHPDIKLTRQADDIAALAALQGELLDALWP
CHHHHHHHCCCCCHHHHHCCCCCCCCCCEEECCCCEEECCHHHHHHHHHHHHHHHHHHHH
TLQVGGMLVYATCSTLPTENTDVIEAFLARTSGARELDIAGQAGQPPAGIKQAHGRQLLA
HHHHCCEEEEEEECCCCCCCHHHHHHHHHHCCCCCEEEECCCCCCCCCCHHHHHHHHHHH
QQGGHDGFYYAKLIKIADQDRGGAGVSA
HCCCCCCHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]