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The map label for this gene is ilvA

Identifier: 62390963

GI number: 62390963

Start: 2214893

End: 2216203

Strand: Reverse

Name: ilvA

Synonym: cg2334

Alternate gene names: 62390963

Gene position: 2216203-2214893 (Counterclockwise)

Preceding gene: 62390965

Following gene: 62390961

Centisome position: 67.51

GC content: 55.3

Gene sequence:

>1311_bases
ATGAGTGAAACATACGTGTCTGAGAAAAGTCCAGGAGTGATGGCTAGCGGAGCGGAGCTGATTCGTGCCGCCGACATTCA
AACGGCGCAGGCACGAATTTCCTCCGTCATTGCACCAACTCCATTGCAGTATTGCCCTCGTCTTTCTGAGGAAACCGGAG
CGGAAATCTACCTTAAGCGTGAGGATCTGCAGGATGTTCGTTCCTACAAGATCCGCGGTGCGCTGAACTCTGGAGCGCAG
CTCACCCAAGAGCAGCGCGATGCAGGTATCGTTGCCGCATCTGCAGGTAACCATGCCCAGGGCGTGGCCTATGTGTGCAA
GTCCTTGGGCGTTCAGGGACGCATCTATGTTCCTGTGCAGACTCCAAAGCAAAAGCGTGACCGCATCATGGTTCACGGCG
GAGAGTTTGTCTCCTTGGTGGTCACTGGCAATAACTTCGACGAAGCATCGGCTGCAGCGCATGAAGATGCAGAGCGCACC
GGCGCAACGCTGATCGAGCCTTTCGATGCTCGCAACACCGTCATCGGTCAGGGCACCGTGGCTGCTGAGATCTTGTCGCA
GCTGACTTCCATGGGCAAGAGTGCAGATCACGTGATGGTTCCAGTCGGCGGTGGCGGACTTCTTGCAGGTGTGGTCAGCT
ACATGGCTGATATGGCACCTCGCACTGCGATCGTTGGTATCGAACCAGCGGGAGCAGCATCCATGCAGGCTGCATTGCAC
AATGGTGGACCAATCACTTTGGAGACTGTTGATCCCTTTGTGGACGGCGCAGCAGTCAAACGTGTCGGAGATCTCAACTA
CACCATCGTGGAGAAGAACCAGGGTCGCGTGCACATGATGAGCGCGACCGAGGGCGCTGTGTGTACTGAGATGCTCGATC
TTTACCAAAACGAAGGCATCATCGCGGAGCCTGCTGGCGCGCTGTCTATCGCTGGGTTGAAGGAAATGTCCTTTGCACCT
GGTTCTGTCGTGGTGTGCATCATCTCTGGTGGCAACAACGATGTGCTGCGTTATGCGGAAATCGCTGAGCGCTCCTTGGT
GCACCGCGGTTTGAAGCACTACTTCTTGGTGAACTTCCCGCAAAAGCCTGGTCAGTTGCGTCACTTCCTGGAAGATATCC
TGGGACCGGATGATGACATCACGCTGTTTGAGTACCTCAAGCGCAACAACCGTGAGACCGGTACTGCGTTGGTGGGTATT
CACTTGAGTGAAGCATCAGGATTGGATTCTTTGCTGGAACGTATGGAGGAATCGGCAATTGATTCCCGTCGCCTCGAGCC
GGGCACCCCTGAGTACGAATACTTGACCTAA

Upstream 100 bases:

>100_bases
CTAGAAAACAATCTATAGCATTGTTCTACAAAGAGCTTGTTGGAAATAAAACCTATGCCAAAGTAGGTGCAATTCTAGGA
GAAGATTACACTAGTCAACC

Downstream 100 bases:

>100_bases
ACATAGCTGAAGGCCACCTCAATCGAGGTGGCCTTTTTCTAGTTTCGGGTCAGGATCGCAAAGCCCCACGGCTGAAGGGT
TGTGGAGGTGTCGGTGACGG

Product: threonine dehydratase

Products: NA

Alternate protein names: Threonine deaminase

Number of amino acids: Translated: 436; Mature: 435

Protein sequence:

>436_residues
MSETYVSEKSPGVMASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRGALNSGAQ
LTQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERT
GATLIEPFDARNTVIGQGTVAAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALH
NGGPITLETVDPFVDGAAVKRVGDLNYTIVEKNQGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGLKEMSFAP
GSVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGI
HLSEASGLDSLLERMEESAIDSRRLEPGTPEYEYLT

Sequences:

>Translated_436_residues
MSETYVSEKSPGVMASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRGALNSGAQ
LTQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERT
GATLIEPFDARNTVIGQGTVAAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALH
NGGPITLETVDPFVDGAAVKRVGDLNYTIVEKNQGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGLKEMSFAP
GSVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGI
HLSEASGLDSLLERMEESAIDSRRLEPGTPEYEYLT
>Mature_435_residues
SETYVSEKSPGVMASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKREDLQDVRSYKIRGALNSGAQL
TQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQTPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERTG
ATLIEPFDARNTVIGQGTVAAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALHN
GGPITLETVDPFVDGAAVKRVGDLNYTIVEKNQGRVHMMSATEGAVCTEMLDLYQNEGIIAEPAGALSIAGLKEMSFAPG
SVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFPQKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGIH
LSEASGLDSLLERMEESAIDSRRLEPGTPEYEYLT

Specific function: Catalyzes the formation of alpha-ketobutyrate from threonine in a two-step reaction. The first step is a dehydration of threonine, followed by rehydration and liberation of ammonia. Deaminates L-threonine, but also L-serine to a lesser extent

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the serine/threonine dehydratase family

Homologues:

Organism=Homo sapiens, GI11345492, Length=335, Percent_Identity=27.7611940298507, Blast_Score=115, Evalue=1e-25,
Organism=Escherichia coli, GI1790207, Length=392, Percent_Identity=35.969387755102, Blast_Score=193, Evalue=1e-50,
Organism=Escherichia coli, GI1789505, Length=320, Percent_Identity=33.4375, Blast_Score=150, Evalue=2e-37,
Organism=Escherichia coli, GI1789235, Length=244, Percent_Identity=25.8196721311475, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI71991565, Length=347, Percent_Identity=30.2593659942363, Blast_Score=115, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17537387, Length=399, Percent_Identity=29.3233082706767, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17508781, Length=283, Percent_Identity=29.3286219081272, Blast_Score=110, Evalue=1e-24,
Organism=Saccharomyces cerevisiae, GI6320930, Length=376, Percent_Identity=35.3723404255319, Blast_Score=196, Evalue=4e-51,
Organism=Saccharomyces cerevisiae, GI6322631, Length=320, Percent_Identity=31.875, Blast_Score=138, Evalue=2e-33,
Organism=Saccharomyces cerevisiae, GI6319788, Length=236, Percent_Identity=27.5423728813559, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI21355833, Length=345, Percent_Identity=31.0144927536232, Blast_Score=106, Evalue=3e-23,
Organism=Drosophila melanogaster, GI24645328, Length=228, Percent_Identity=30.7017543859649, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): THD1_CORGL (Q04513)

Other databases:

- EMBL:   L01508
- EMBL:   BA000036
- EMBL:   BX927154
- PIR:   A47044
- RefSeq:   NP_601328.2
- RefSeq:   YP_226365.1
- ProteinModelPortal:   Q04513
- SMR:   Q04513
- GeneID:   1020078
- GeneID:   3342699
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg2334
- KEGG:   cgl:NCgl2046
- HOGENOM:   HBG714501
- OMA:   VDPFVDG
- PhylomeDB:   Q04513
- ProtClustDB:   PRK08639
- BioCyc:   CGLU196627:CG2334-MONOMER
- BRENDA:   4.3.1.19
- InterPro:   IPR001926
- InterPro:   IPR000634
- InterPro:   IPR001721
- InterPro:   IPR011820
- TIGRFAMs:   TIGR02079

Pfam domain/function: PF00291 PALP; PF00585 Thr_dehydrat_C; SSF53686 PyrdxlP-dep_enz_bsu

EC number: =4.3.1.19

Molecular weight: Translated: 46572; Mature: 46441

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSETYVSEKSPGVMASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKR
CCCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEH
EDLQDVRSYKIRGALNSGAQLTQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQ
HHHHHHHHEEEECCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
TPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNTVIGQGTV
CCHHCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCEEEECCHH
AAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALH
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
NGGPITLETVDPFVDGAAVKRVGDLNYTIVEKNQGRVHMMSATEGAVCTEMLDLYQNEGI
CCCCEEEEECCCHHCCHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCE
IAEPAGALSIAGLKEMSFAPGSVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFP
EECCCCCEEECCCHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECC
QKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLERMEESAI
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
DSRRLEPGTPEYEYLT
HHCCCCCCCCCCCCCC
>Mature Secondary Structure 
SETYVSEKSPGVMASGAELIRAADIQTAQARISSVIAPTPLQYCPRLSEETGAEIYLKR
CCCCCCCCCCCCEECCHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCCCCCCCEEEEEH
EDLQDVRSYKIRGALNSGAQLTQEQRDAGIVAASAGNHAQGVAYVCKSLGVQGRIYVPVQ
HHHHHHHHEEEECCCCCCHHHHHHHHCCCEEEECCCCCCHHHHHHHHHHCCCCEEEEECC
TPKQKRDRIMVHGGEFVSLVVTGNNFDEASAAAHEDAERTGATLIEPFDARNTVIGQGTV
CCHHCCCEEEEECCCEEEEEEECCCCCHHHHHHHHHHHHCCCEEECCCCCCCEEEECCHH
AAEILSQLTSMGKSADHVMVPVGGGGLLAGVVSYMADMAPRTAIVGIEPAGAASMQAALH
HHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHH
NGGPITLETVDPFVDGAAVKRVGDLNYTIVEKNQGRVHMMSATEGAVCTEMLDLYQNEGI
CCCCEEEEECCCHHCCHHHHHHCCCEEEEEECCCCEEEEEECCCCHHHHHHHHHHHCCCE
IAEPAGALSIAGLKEMSFAPGSVVVCIISGGNNDVLRYAEIAERSLVHRGLKHYFLVNFP
EECCCCCEEECCCHHCCCCCCCEEEEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEEECC
QKPGQLRHFLEDILGPDDDITLFEYLKRNNRETGTALVGIHLSEASGLDSLLERMEESAI
CCCHHHHHHHHHHCCCCCCHHHHHHHHHCCCCCCEEEEEEEECCCCCHHHHHHHHHHHHH
DSRRLEPGTPEYEYLT
HHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1459955; 12948626