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The map label for this gene is proS [H]

Identifier: 62390834

GI number: 62390834

Start: 2075848

End: 2077614

Strand: Reverse

Name: proS [H]

Synonym: cg2185

Alternate gene names: 62390834

Gene position: 2077614-2075848 (Counterclockwise)

Preceding gene: 62390836

Following gene: 62390828

Centisome position: 63.29

GC content: 58.57

Gene sequence:

>1767_bases
ATGATCACACGTCTTTCCACGCTGTTTTTGCGCACCCTGCGCGAAGACCCTGCAGATGCAGAAGTTCCAAGCCACAAGCT
GCTTGTCCGTGCAGGATATATCCGCCGAGTTGCCCCAGGTATCTACTCCTGGTTGCCACTGGGTTTGCGCGCAGTGCGCA
ACATTGAAGCTGTCGTACGCGAGGAAATGGATGCGATCGGAGGACAGGAGCTGCTCTTCCCAGCACTCCTGCCACGTGAG
CCTTATGAAACCACACAGCGTTGGACAGAATACGGCGATTCGCTGTTCCGTCTGAAGGACCGCAAGGGTGCCGACTACCT
GCTCGGACCAACCCACGAGGAAATGTTCGCCGCCACGGTGAAGGATCTGTACAACTCCTACAAGGACTTCCCAGTCACCT
TGTACCAGATCCAGACCAAGTACCGCGATGAGGAACGCCCACGCGCAGGCGTGCTCCGCGGACGCGAATTTGTGATGAAG
GATTCTTACTCCTTCGACATCTCGGATGCTGGTTTGGACGAGTCCTACGCAAAGCACCGCGCAGCGTATCAGCGCATCTT
TGACCGCCTTGGCTTGGAATACGCCATCTGCCAGGCAACCTCTGGTGCCATGGGCGGATCCGCTTCCGAGGAATTCCTTG
CAGTATCTGAAAACGGCGAAGATACCTTCGTGCGCTCTACCTCCGGCAATTACGCCGCAAACGTGGAAGCTGTTGTTACC
CAGCCAGGCGTTGAGCGTGACATCGAAGGCCTGCCAGAAGCAGTCACCTACGAAACTCCTGTTTCTGAAACCATCGATGC
TTTGGTTGATTGGGCTAACTCCATCGACGTGCAGATCGAGGGCCGCGAGGTCACCGCAGCTGACACCCTCAAGTGCATCG
TGGTGAAGGTCCGCGAGCCAGGTGCCGAAGAAGCAGAACTCACTGGAATCTTGCTTCCAGGTGACCGCGAAGTAGACATG
AAGCGCCTCGAGGCATCACTTGAGCCAGCAGAAGTTGAACTCGCAGTGGAATCCGACTTTGCCGACAACCCATTCCTGGT
CAAGGGCTACGTCGGACCAGTTGGCCTGGCCAAGAACGGCGTGAAGGTCCTTGCCGATCCTCGCGTTGTCACCGGTACCT
CCTGGATCACCGGCGCCGATGAAAAGGAACGCCACGTCGTAGGCCTCGTCGCCGGACGCGATTTCACCCCAGACGGCTTC
ATCGAAGCTGCAGAAATCAAGGAAGGCGACCCAGCGCCAGCAGGCGAGGGCACCCTCACCCTTGCTCGCGGCATCGAAAT
TGGCCATATCTTCCAGCTCGGCCGCAAGTACACCGAAGCCTTCGACGTCCAAATCCTGGACGAAAACGGCAAGCGCGCCA
TCCCAACCATGGGCTCCTACGGCCTCGGTGTCACCCGCCTGCTCGCCGTCCTGGCAGAACAGCGCCACGATGACGCTGGC
CTCAACTGGTCCGTCGAAGTCGCTCCGTACCAGGTCCATGTCGTCGCAGCTAACAAAGACGCCGCAGCCATCGAGGCAGC
AGAGCGCTTTGCGGCTGAGCTGTCTGCCGCAGGCTTGGACGTGCTTTTCGACGACCGACCCAAGGTTAGCCCTGGCGTAA
AGTTCAAAGACGCCGAACTTCTTGGCATGCCATTCGCCTTGATTCTTGGCCGTGGCTACGCCGAAGGCAAGGTTGAACTC
CGTGTCCGCGGTGGCGAGAAGTCCGAACTCGACGCAGATCAGGCTGTCGCCCAGATCGTCGAGATGGTTGCTCAAGCCCG
CAACTAG

Upstream 100 bases:

>100_bases
ACACAATAAGCATGCATGAGAGGATACTGTAAAAGCCTTGCCTCTTAAATCGAGGGGGCAGGGCAGTCGAAGCCAAGTTG
AGAGGGTATATTTGTCACCT

Downstream 100 bases:

>100_bases
TTCTTCTGAACAATAAAGCTCTGCCCCGGTTCAAGATCGAATTTCTTGAACCGGGGCAGAAGTGTTTTTGGGTTTAGTGA
GGCTGTGCGGGTTAAACCAG

Product: prolyl-tRNA synthetase

Products: NA

Alternate protein names: Proline--tRNA ligase; ProRS [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRVAPGIYSWLPLGLRAVRNIEAVVREEMDAIGGQELLFPALLPRE
PYETTQRWTEYGDSLFRLKDRKGADYLLGPTHEEMFAATVKDLYNSYKDFPVTLYQIQTKYRDEERPRAGVLRGREFVMK
DSYSFDISDAGLDESYAKHRAAYQRIFDRLGLEYAICQATSGAMGGSASEEFLAVSENGEDTFVRSTSGNYAANVEAVVT
QPGVERDIEGLPEAVTYETPVSETIDALVDWANSIDVQIEGREVTAADTLKCIVVKVREPGAEEAELTGILLPGDREVDM
KRLEASLEPAEVELAVESDFADNPFLVKGYVGPVGLAKNGVKVLADPRVVTGTSWITGADEKERHVVGLVAGRDFTPDGF
IEAAEIKEGDPAPAGEGTLTLARGIEIGHIFQLGRKYTEAFDVQILDENGKRAIPTMGSYGLGVTRLLAVLAEQRHDDAG
LNWSVEVAPYQVHVVAANKDAAAIEAAERFAAELSAAGLDVLFDDRPKVSPGVKFKDAELLGMPFALILGRGYAEGKVEL
RVRGGEKSELDADQAVAQIVEMVAQARN

Sequences:

>Translated_588_residues
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRVAPGIYSWLPLGLRAVRNIEAVVREEMDAIGGQELLFPALLPRE
PYETTQRWTEYGDSLFRLKDRKGADYLLGPTHEEMFAATVKDLYNSYKDFPVTLYQIQTKYRDEERPRAGVLRGREFVMK
DSYSFDISDAGLDESYAKHRAAYQRIFDRLGLEYAICQATSGAMGGSASEEFLAVSENGEDTFVRSTSGNYAANVEAVVT
QPGVERDIEGLPEAVTYETPVSETIDALVDWANSIDVQIEGREVTAADTLKCIVVKVREPGAEEAELTGILLPGDREVDM
KRLEASLEPAEVELAVESDFADNPFLVKGYVGPVGLAKNGVKVLADPRVVTGTSWITGADEKERHVVGLVAGRDFTPDGF
IEAAEIKEGDPAPAGEGTLTLARGIEIGHIFQLGRKYTEAFDVQILDENGKRAIPTMGSYGLGVTRLLAVLAEQRHDDAG
LNWSVEVAPYQVHVVAANKDAAAIEAAERFAAELSAAGLDVLFDDRPKVSPGVKFKDAELLGMPFALILGRGYAEGKVEL
RVRGGEKSELDADQAVAQIVEMVAQARN
>Mature_588_residues
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRVAPGIYSWLPLGLRAVRNIEAVVREEMDAIGGQELLFPALLPRE
PYETTQRWTEYGDSLFRLKDRKGADYLLGPTHEEMFAATVKDLYNSYKDFPVTLYQIQTKYRDEERPRAGVLRGREFVMK
DSYSFDISDAGLDESYAKHRAAYQRIFDRLGLEYAICQATSGAMGGSASEEFLAVSENGEDTFVRSTSGNYAANVEAVVT
QPGVERDIEGLPEAVTYETPVSETIDALVDWANSIDVQIEGREVTAADTLKCIVVKVREPGAEEAELTGILLPGDREVDM
KRLEASLEPAEVELAVESDFADNPFLVKGYVGPVGLAKNGVKVLADPRVVTGTSWITGADEKERHVVGLVAGRDFTPDGF
IEAAEIKEGDPAPAGEGTLTLARGIEIGHIFQLGRKYTEAFDVQILDENGKRAIPTMGSYGLGVTRLLAVLAEQRHDDAG
LNWSVEVAPYQVHVVAANKDAAAIEAAERFAAELSAAGLDVLFDDRPKVSPGVKFKDAELLGMPFALILGRGYAEGKVEL
RVRGGEKSELDADQAVAQIVEMVAQARN

Specific function: Catalyzes the attachment of proline to tRNA(Pro) in a two-step reaction:proline is first activated by ATP to form Pro- AMP and then transferred to the acceptor end of tRNA(Pro). As ProRS can inadvertently accommodate and process non-cognate amino acids su

COG id: COG0442

COG function: function code J; Prolyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. ProS type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI34303926, Length=228, Percent_Identity=40.7894736842105, Blast_Score=187, Evalue=3e-47,
Organism=Escherichia coli, GI1786392, Length=585, Percent_Identity=39.1452991452991, Blast_Score=399, Evalue=1e-112,
Organism=Caenorhabditis elegans, GI115532348, Length=219, Percent_Identity=36.0730593607306, Blast_Score=127, Evalue=2e-29,
Organism=Saccharomyces cerevisiae, GI6320931, Length=584, Percent_Identity=28.9383561643836, Blast_Score=236, Evalue=7e-63,
Organism=Drosophila melanogaster, GI24656200, Length=216, Percent_Identity=36.5740740740741, Blast_Score=153, Evalue=3e-37,

Paralogues:

None

Copy number: 800 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR002316
- InterPro:   IPR004500
- InterPro:   IPR007214 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b; PF04073 YbaK [H]

EC number: =6.1.1.15 [H]

Molecular weight: Translated: 64332; Mature: 64332

Theoretical pI: Translated: 4.47; Mature: 4.47

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRVAPGIYSWLPLGLRAVRNIEAVVR
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEMDAIGGQELLFPALLPREPYETTQRWTEYGDSLFRLKDRKGADYLLGPTHEEMFAATV
HHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEECCCHHHHHHHHH
KDLYNSYKDFPVTLYQIQTKYRDEERPRAGVLRGREFVMKDSYSFDISDAGLDESYAKHR
HHHHHHHCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHH
AAYQRIFDRLGLEYAICQATSGAMGGSASEEFLAVSENGEDTFVRSTSGNYAANVEAVVT
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEECEEEEEE
QPGVERDIEGLPEAVTYETPVSETIDALVDWANSIDVQIEGREVTAADTLKCIVVKVREP
CCCCHHHHHCCCCCEEECCCHHHHHHHHHHCCCCEEEEEECCEEEHHCCEEEEEEEECCC
GAEEAELTGILLPGDREVDMKRLEASLEPAEVELAVESDFADNPFLVKGYVGPVGLAKNG
CCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCC
VKVLADPRVVTGTSWITGADEKERHVVGLVAGRDFTPDGFIEAAEIKEGDPAPAGEGTLT
CEEEECCEEEECCCEECCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEE
LARGIEIGHIFQLGRKYTEAFDVQILDENGKRAIPTMGSYGLGVTRLLAVLAEQRHDDAG
EECCCCHHHHHHHHHHHHHEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCC
LNWSVEVAPYQVHVVAANKDAAAIEAAERFAAELSAAGLDVLFDDRPKVSPGVKFKDAEL
CCEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCHH
LGMPFALILGRGYAEGKVELRVRGGEKSELDADQAVAQIVEMVAQARN
HCCCHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MITRLSTLFLRTLREDPADAEVPSHKLLVRAGYIRRVAPGIYSWLPLGLRAVRNIEAVVR
CCCHHHHHHHHHHHCCCCCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EEMDAIGGQELLFPALLPREPYETTQRWTEYGDSLFRLKDRKGADYLLGPTHEEMFAATV
HHHHHCCCCHHHHHHHCCCCCHHHHHHHHHHCCCEEEECCCCCCCCEECCCHHHHHHHHH
KDLYNSYKDFPVTLYQIQTKYRDEERPRAGVLRGREFVMKDSYSFDISDAGLDESYAKHR
HHHHHHHCCCCEEEEEEEHHHCCCCCCCHHHHCCCEEEEECCCCCCCCCCCCCHHHHHHH
AAYQRIFDRLGLEYAICQATSGAMGGSASEEFLAVSENGEDTFVRSTSGNYAANVEAVVT
HHHHHHHHHCCCEEEEEECCCCCCCCCCCCCEEEEECCCCCEEEEECCCCEEECEEEEEE
QPGVERDIEGLPEAVTYETPVSETIDALVDWANSIDVQIEGREVTAADTLKCIVVKVREP
CCCCHHHHHCCCCCEEECCCHHHHHHHHHHCCCCEEEEEECCEEEHHCCEEEEEEEECCC
GAEEAELTGILLPGDREVDMKRLEASLEPAEVELAVESDFADNPFLVKGYVGPVGLAKNG
CCCCCCEEEEECCCCCCHHHHHHHCCCCCCEEEEEEECCCCCCCEEEEECCCCCCCCCCC
VKVLADPRVVTGTSWITGADEKERHVVGLVAGRDFTPDGFIEAAEIKEGDPAPAGEGTLT
CEEEECCEEEECCCEECCCCCCCCEEEEEEECCCCCCCCCEEEECCCCCCCCCCCCCCEE
LARGIEIGHIFQLGRKYTEAFDVQILDENGKRAIPTMGSYGLGVTRLLAVLAEQRHDDAG
EECCCCHHHHHHHHHHHHHEEEEEEECCCCCEECCCCCCCCCCHHHHHHHHHHHCCCCCC
LNWSVEVAPYQVHVVAANKDAAAIEAAERFAAELSAAGLDVLFDDRPKVSPGVKFKDAEL
CCEEEEECCEEEEEEEECCCHHHHHHHHHHHHHHHHCCCEEEECCCCCCCCCCEECCCHH
LGMPFALILGRGYAEGKVELRVRGGEKSELDADQAVAQIVEMVAQARN
HCCCHHHHHCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA