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The map label for this gene is miaA [H]

Identifier: 62390784

GI number: 62390784

Start: 2022730

End: 2023635

Strand: Reverse

Name: miaA [H]

Synonym: cg2130

Alternate gene names: 62390784

Gene position: 2023635-2022730 (Counterclockwise)

Preceding gene: 62390785

Following gene: 62390783

Centisome position: 61.65

GC content: 56.18

Gene sequence:

>906_bases
GTGGTAACACCGATCGCAGTGGTTGGACCCACTGCATCTGGAAAATCAGCTTTGGGAATTGCTCTAGCCCACAAGCTTGA
CGGTGAAGTAGTCAATGTGGATTCCATGCAGCTGTACAAAGGCATGGACATCGGCACGGCAAAGCTGACTGTCGAAGAAC
GCGAAGGCATTGCGCATCATCAGCTCGATGTCTGGGACGTTACCGAAACTGCGTCAGTGGCGAGGTTTCAATCCGACGCC
GTTGCCGATGTGGAAGATATTATGTCCCGTGGCAAAACCCCCATCTTGGTTGGCGGCTCCATGTTGTACGTCCAATCTTT
GGTCGATGATTGGCAATTCCCACCTACCGACAGCGCTGTTCGCGCACGCTTTGAGGCCCGCTTGGCAGACATCGGTGTCG
AAGCACTACACGCTGAACTTACTCAGCTTGACCCAGAAGCAGCAGCCGTCATCGAAAGCAATGATCCCCGACGCACCGTC
CGAGCATTAGAAGTCATTGAACTAACCGGCCAGCCCTTCCAAGCAAGCCAACCGCCCAAAGACGCGCCACCTCGCTGGGG
AACTCGAATCATTGGCCTGAAAACCACTCCAGAATGGCTAAATCCACGCATCGAGCAGCGCACCGCCAGGATGTTTGAAC
AAGGCTTTGTCGCCGAAGTGGAACACCTTGTGCAGCAAGGACTCATCGCTGACTCCACCGCGGGACGAGCAATCGGCTAC
TCCCAAGTACTGGCAGCCATGGCAGGGGAGATGACCTGGGAAGACGCCTTCGAACGCACGGTCACCGGAACCAGACGCTA
TGTCAGGCGCCAACGCAGCTGGTTCAACAGAGACCACCGCGTGTCCTGGGTCGACGCCTCTGGCGATCCCACCGCACAAG
CCTTGGAGATTTTGGGTCTACAATAG

Upstream 100 bases:

>100_bases
ACAGAAGCGTCAACTGCTCAACTTCTCGACAGCGACGACACACACGGCGTGGGACGCTACGTTGCTCGCTGGACTTTTTA
ACTTAAGGGAGATCTAGATC

Downstream 100 bases:

>100_bases
CGAGGGTGAATTTGACCATCCCCTTTGCCAAAGGCCACGCCACCGAAAACGACTTCATCATCATCCCCGATGAGGATGCG
CGCCTAGATTTAACTCCAGA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 301; Mature: 301

Protein sequence:

>301_residues
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHHQLDVWDVTETASVARFQSDA
VADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAVRARFEARLADIGVEALHAELTQLDPEAAAVIESNDPRRTV
RALEVIELTGQPFQASQPPKDAPPRWGTRIIGLKTTPEWLNPRIEQRTARMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGLQ

Sequences:

>Translated_301_residues
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHHQLDVWDVTETASVARFQSDA
VADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAVRARFEARLADIGVEALHAELTQLDPEAAAVIESNDPRRTV
RALEVIELTGQPFQASQPPKDAPPRWGTRIIGLKTTPEWLNPRIEQRTARMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGLQ
>Mature_301_residues
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHHQLDVWDVTETASVARFQSDA
VADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAVRARFEARLADIGVEALHAELTQLDPEAAAVIESNDPRRTV
RALEVIELTGQPFQASQPPKDAPPRWGTRIIGLKTTPEWLNPRIEQRTARMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGLQ

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=307, Percent_Identity=28.9902280130293, Blast_Score=117, Evalue=2e-26,
Organism=Escherichia coli, GI1790613, Length=300, Percent_Identity=40, Blast_Score=201, Evalue=6e-53,
Organism=Caenorhabditis elegans, GI25144712, Length=237, Percent_Identity=32.0675105485232, Blast_Score=118, Evalue=3e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=288, Percent_Identity=32.6388888888889, Blast_Score=150, Evalue=2e-37,
Organism=Drosophila melanogaster, GI24649830, Length=233, Percent_Identity=32.618025751073, Blast_Score=118, Evalue=4e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 33083; Mature: 33083

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHH
CCCCEEEECCCCCCCCHHHHHHHHHCCCCEECCCHHHHHCCCCCCEEEEEEHHHCCCCCC
QLDVWDVTETASVARFQSDAVADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAV
CCEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHH
RARFEARLADIGVEALHAELTQLDPEAAAVIESNDPRRTVRALEVIELTGQPFQASQPPK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DAPPRWGTRIIGLKTTPEWLNPRIEQRTARMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
CCCCCCCCEEEEEECCHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCH
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
Q
C
>Mature Secondary Structure
MVTPIAVVGPTASGKSALGIALAHKLDGEVVNVDSMQLYKGMDIGTAKLTVEEREGIAHH
CCCCEEEECCCCCCCCHHHHHHHHHCCCCEECCCHHHHHCCCCCCEEEEEEHHHCCCCCC
QLDVWDVTETASVARFQSDAVADVEDIMSRGKTPILVGGSMLYVQSLVDDWQFPPTDSAV
CCEEECCHHHHHHHHHHCCHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHCCCCCCCHHH
RARFEARLADIGVEALHAELTQLDPEAAAVIESNDPRRTVRALEVIELTGQPFQASQPPK
HHHHHHHHHHHHHHHHHHHHHHCCCHHHEEECCCCHHHHHHHHHHHHHCCCCCCCCCCCC
DAPPRWGTRIIGLKTTPEWLNPRIEQRTARMFEQGFVAEVEHLVQQGLIADSTAGRAIGY
CCCCCCCCEEEEEECCHHHHCCHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCCCCCCCH
SQVLAAMAGEMTWEDAFERTVTGTRRYVRRQRSWFNRDHRVSWVDASGDPTAQALEILGL
HHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCCHHHHHHHHCC
Q
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA