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The map label for this gene is prfC [H]

Identifier: 62389819

GI number: 62389819

Start: 986331

End: 988079

Strand: Reverse

Name: prfC [H]

Synonym: cg1060

Alternate gene names: 62389819

Gene position: 988079-986331 (Counterclockwise)

Preceding gene: 62389825

Following gene: 62389817

Centisome position: 30.1

GC content: 61.52

Gene sequence:

>1749_bases
GTGGCAAAAAACGTCGACAAGCAGGCAACGCCGGCGTACAACTTCGCTGAGCTGGGGCGACTATGGCCCAGCGCCCACAA
CCCGCTATTCTTAATACCCATGAGCAACGCCAATCCCGACACCACCGCCGCCGAGGCACATCGCCGCAGAACATTCGCCG
TAATCGCACACCCCGACGCCGGTAAATCCACCCTCACCGAGGCATTGGCGCTGCATGCACACATCATCTCCGAAGCCGGC
GCCACCCACGGCAAAGCAGGCCGCAAAGCCACCGTTTCCGACTGGATGGAAATGGAAAAAGACCGCGGCATCTCCATCGC
CTCCTCCGCACTCCAATTCGAGTACGCACCAGAAGGCCACGCAGGCGAGCCCTTCATGATCAACCTCGTGGACACCCCAG
GCCACGCCGACTTCTCCGAAGACACCTACCGCGTCCTCATGGCCGTCGACGCAGCAGTCATGCTTATCGACGCCGCCAAA
GGCCTCGAACCCCAAACCCTCCAGCTCTTCCGCGTCTGCAAAGCCCGCGGCCTGCCAATCATCACCGTGATCAACAAATG
GGACCGCGTCGGCCGCACCCCACTCGAGCTCGTCGACGAAATCGTCAAAGAAATCGAACTCCAACCGACCCCACTGTTCT
GGCCAGTCGGTGAAGCCGGCGACTTCCGCGGCCTAGCCCGCATCAACAACGACGGCGAAGTCGAAGAATACATCCACTTC
ACCCGCACCGCCGGCGGCTCCACCATTGCCCCCGAAGACTTCTACACCCCAGAAGAAGCCATCGAACGCGAAGGCGACGT
CTGGGAAAACGCCACCGAAGAAGCAGAACTCCTCGCAGCCGACGGCGCCGTCCACGACCAGGAACTCTTCCTCAACTGCA
CCACCTCCCCACTGATCTTCGCCTCCGCGATGCTCAACTTCGGCGTCCACCAAATCCTGGACACCCTCTGCCAACTCGCA
CCATCCCCCGCCGGCCGCGACGCAGACCCCAAAGCCCTCGAAGCCGCCACCTCCGCAATGGACGACCACCGCGACACCAC
CGACGACTTCTCCGGCGTCGTCTTCAAAGTCCAAGCCGGCATGGACAAAAACCACCGCGATACCCTCGCCTTCATGCGCG
TCGTCTCCGGCGAATTCGACCGCGGCATGCAAGTCACCCACTCCCAATCCGGCCGCAGCTTCTCCACCAAATACGCCCTC
ACCGTCTTCGGCCGCACCCGCTCTACCGTCGAAACCGCCTTCCCCGGCGACATCGTCGGCCTCGTCAACGCCGGCGCCCT
CGCACCAGGCGACACCATCTTCGAAGGCCGAAAAATCCAATACCCACCAATGCCAAAATTCGCGCCAGAACACTTCCGCA
TCCTGCGCGCCAAATCACTCGGCAAATACAAACAGTTCCGCAAAGCCCTCGAGCAGCTGGACTCCGAAGGTGTCGTCCAG
ATCCTCAAGAACGACCTGCGTGGCGACGCCAACCCAGTCATGGCCGCTGTCGGCCCAATGCAGTTCGAAGTGATGCAAGC
CCGCATGGAAGTCGAATACAACGTCGAAACCGTCGCCGATCCGATTCCATACTCCGTCGCCCGCCGCACCACCGCCGAAA
CCGCACCGGAACTGGCGAAGCAGCGCGGCGTGGAAATCTTCACCCGCACCGACGGCGAACTGATTGCACTGTTCAGCGAC
AAGTGGCGCCTATCCTTCATCGAGAAGGAGCACCCTGATTTCGTGCTGGAGTCGCTGGTAGCTGAGTAA

Upstream 100 bases:

>100_bases
CTCTATAGTTTTTAGAGTTTCTGTCAGCCTATAGGTAGTTGTTTTTGAGCTTAAATGTAGTGACGTGTCTTACATTTCAG
CCACCCCCGAATTAAGGGAA

Downstream 100 bases:

>100_bases
TTAACTGGGCGGCTTTGGTGGCTTGGGCTGCTTGGGATTTTTGCTTGTCGACGTCAGGTTCCAGGTGGCCCGGGCCCGGC
TGGCCAGTTCGGGGTGTGCT

Product: peptide chain release factor 3

Products: NA

Alternate protein names: RF-3 [H]

Number of amino acids: Translated: 582; Mature: 581

Protein sequence:

>582_residues
MAKNVDKQATPAYNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDAGKSTLTEALALHAHIISEAG
ATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGHAGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAK
GLEPQTLQLFRVCKARGLPIITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEVEEYIHF
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIFASAMLNFGVHQILDTLCQLA
PSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAGMDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYAL
TVFGRTRSTVETAFPGDIVGLVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAKQRGVEIFTRTDGELIALFSD
KWRLSFIEKEHPDFVLESLVAE

Sequences:

>Translated_582_residues
MAKNVDKQATPAYNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDAGKSTLTEALALHAHIISEAG
ATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGHAGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAK
GLEPQTLQLFRVCKARGLPIITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEVEEYIHF
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIFASAMLNFGVHQILDTLCQLA
PSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAGMDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYAL
TVFGRTRSTVETAFPGDIVGLVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAKQRGVEIFTRTDGELIALFSD
KWRLSFIEKEHPDFVLESLVAE
>Mature_581_residues
AKNVDKQATPAYNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDAGKSTLTEALALHAHIISEAGA
THGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGHAGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAKG
LEPQTLQLFRVCKARGLPIITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEVEEYIHFT
RTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIFASAMLNFGVHQILDTLCQLAP
SPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAGMDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYALT
VFGRTRSTVETAFPGDIVGLVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQI
LKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAKQRGVEIFTRTDGELIALFSDK
WRLSFIEKEHPDFVLESLVAE

Specific function: Increases the formation of ribosomal termination complexes and stimulates activities of RF-1 and RF-2. It binds guanine nucleotides and has strong preference for UGA stop codons. It may interact directly with the ribosome. The stimulation of RF- 1 and RF-

COG id: COG4108

COG function: function code J; Peptide chain release factor RF-3

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. PrfC subfamily [H]

Homologues:

Organism=Homo sapiens, GI18390331, Length=529, Percent_Identity=24.7637051039698, Blast_Score=149, Evalue=8e-36,
Organism=Homo sapiens, GI19923640, Length=575, Percent_Identity=26.9565217391304, Blast_Score=144, Evalue=2e-34,
Organism=Homo sapiens, GI25306283, Length=412, Percent_Identity=28.6407766990291, Blast_Score=134, Evalue=3e-31,
Organism=Homo sapiens, GI25306287, Length=306, Percent_Identity=31.3725490196078, Blast_Score=120, Evalue=3e-27,
Organism=Homo sapiens, GI157426893, Length=158, Percent_Identity=32.9113924050633, Blast_Score=91, Evalue=2e-18,
Organism=Homo sapiens, GI94966754, Length=141, Percent_Identity=35.4609929078014, Blast_Score=91, Evalue=4e-18,
Organism=Homo sapiens, GI4503483, Length=149, Percent_Identity=34.8993288590604, Blast_Score=85, Evalue=2e-16,
Organism=Homo sapiens, GI310132016, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI310110807, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=8e-15,
Organism=Homo sapiens, GI310123363, Length=116, Percent_Identity=37.0689655172414, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI1790835, Length=521, Percent_Identity=40.4990403071017, Blast_Score=376, Evalue=1e-105,
Organism=Escherichia coli, GI1789738, Length=529, Percent_Identity=26.8431001890359, Blast_Score=149, Evalue=7e-37,
Organism=Escherichia coli, GI1788922, Length=152, Percent_Identity=35.5263157894737, Blast_Score=97, Evalue=4e-21,
Organism=Escherichia coli, GI48994988, Length=190, Percent_Identity=32.6315789473684, Blast_Score=96, Evalue=6e-21,
Organism=Escherichia coli, GI1789737, Length=137, Percent_Identity=32.8467153284672, Blast_Score=62, Evalue=9e-11,
Organism=Escherichia coli, GI1790412, Length=137, Percent_Identity=32.8467153284672, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17533571, Length=500, Percent_Identity=24.8, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI17556745, Length=502, Percent_Identity=24.5019920318725, Blast_Score=111, Evalue=1e-24,
Organism=Caenorhabditis elegans, GI17557151, Length=192, Percent_Identity=31.7708333333333, Blast_Score=87, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI71988819, Length=145, Percent_Identity=34.4827586206897, Blast_Score=83, Evalue=5e-16,
Organism=Caenorhabditis elegans, GI71988811, Length=145, Percent_Identity=34.4827586206897, Blast_Score=82, Evalue=7e-16,
Organism=Caenorhabditis elegans, GI17506493, Length=182, Percent_Identity=31.8681318681319, Blast_Score=79, Evalue=8e-15,
Organism=Saccharomyces cerevisiae, GI6323098, Length=499, Percent_Identity=24.0480961923848, Blast_Score=142, Evalue=1e-34,
Organism=Saccharomyces cerevisiae, GI6322359, Length=553, Percent_Identity=24.9547920433996, Blast_Score=122, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6323320, Length=158, Percent_Identity=34.1772151898734, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6324707, Length=149, Percent_Identity=37.5838926174497, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320593, Length=149, Percent_Identity=37.5838926174497, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324166, Length=274, Percent_Identity=27.7372262773723, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24582462, Length=538, Percent_Identity=24.7211895910781, Blast_Score=149, Evalue=8e-36,
Organism=Drosophila melanogaster, GI221458488, Length=539, Percent_Identity=24.1187384044527, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI78706572, Length=161, Percent_Identity=36.0248447204969, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI28574573, Length=256, Percent_Identity=28.515625, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585709, Length=153, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585711, Length=153, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-15,
Organism=Drosophila melanogaster, GI24585713, Length=153, Percent_Identity=33.3333333333333, Blast_Score=80, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR004548
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU [H]

EC number: NA

Molecular weight: Translated: 64060; Mature: 63929

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKNVDKQATPAYNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDA
CCCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEECCCC
GKSTLTEALALHAHIISEAGATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHEEEEECCCCC
AGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAKGLEPQTLQLFRVCKARGLPI
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
ITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEVEEYIHF
EEEEHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHH
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIF
HHCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHHHEECCCCCCCCEEEEECCCCHHHH
ASAMLNFGVHQILDTLCQLAPSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
MDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYALTVFGRTRSTVETAFPGDIVG
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHCCCCCHHH
LVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
HHCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAK
HHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHEECHHHHCCCCCHHHHHHHHHHHHHHHHH
QRGVEIFTRTDGELIALFSDKWRLSFIEKEHPDFVLESLVAE
HCCCEEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHHHCC
>Mature Secondary Structure 
AKNVDKQATPAYNFAELGRLWPSAHNPLFLIPMSNANPDTTAAEAHRRRTFAVIAHPDA
CCCCCCCCCCCCCHHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHCCCEEEEEECCCC
GKSTLTEALALHAHIISEAGATHGKAGRKATVSDWMEMEKDRGISIASSALQFEYAPEGH
CHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHCCCCEEEHHHEEEEECCCCC
AGEPFMINLVDTPGHADFSEDTYRVLMAVDAAVMLIDAAKGLEPQTLQLFRVCKARGLPI
CCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCEE
ITVINKWDRVGRTPLELVDEIVKEIELQPTPLFWPVGEAGDFRGLARINNDGEVEEYIHF
EEEEHHHHHCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCEEECCCCCHHHHHHH
TRTAGGSTIAPEDFYTPEEAIEREGDVWENATEEAELLAADGAVHDQELFLNCTTSPLIF
HHCCCCCCCCCCCCCCCHHHHHCCCCHHCCCCHHHHHEECCCCCCCCEEEEECCCCHHHH
ASAMLNFGVHQILDTLCQLAPSPAGRDADPKALEAATSAMDDHRDTTDDFSGVVFKVQAG
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
MDKNHRDTLAFMRVVSGEFDRGMQVTHSQSGRSFSTKYALTVFGRTRSTVETAFPGDIVG
CCCCCHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCEEEEEEECCCCCHHHHCCCCCHHH
LVNAGALAPGDTIFEGRKIQYPPMPKFAPEHFRILRAKSLGKYKQFRKALEQLDSEGVVQ
HHCCCCCCCCCCCCCCCEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
ILKNDLRGDANPVMAAVGPMQFEVMQARMEVEYNVETVADPIPYSVARRTTAETAPELAK
HHHHHHCCCCCCHHHHCCCHHHHHHHHHHHHEECHHHHCCCCCHHHHHHHHHHHHHHHHH
QRGVEIFTRTDGELIALFSDKWRLSFIEKEHPDFVLESLVAE
HCCCEEEEECCCCEEEEECCCEEEEECCCCCHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA