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The map label for this gene is mscL [H]

Identifier: 62389768

GI number: 62389768

Start: 934037

End: 934444

Strand: Reverse

Name: mscL [H]

Synonym: cg1001

Alternate gene names: 62389768

Gene position: 934444-934037 (Counterclockwise)

Preceding gene: 62389769

Following gene: 62389760

Centisome position: 28.47

GC content: 53.19

Gene sequence:

>408_bases
ATGCTTAAAGGTTTTAGAGATTTCGTCCTTCGCGGAAATGTCATTGAACTCGCAGTTGCCGTGGTCATCGGTACTGCCTT
CACCGCTATCGTGACAGCATTCTCCGAGAGCATCATCAACCCATTGATCGCTTCCATCGGCAGCACAGAGGTTGAAGGCC
TCGGCTTCCACATCCGCGCCGGCAATGCCGCAACATTCGTGGATTTTGGTGCTGTCATCACCGCAGCGATCAACTTCCTC
ATCATCGCAGCAATTGTCTACTTCGTCCTCGTTGCTCCAATGAACAAGCTCAGCGAAACCCTCGCAAAGCGCAAGGGTGT
TGAAGAAGACGAGACCCCAGCTTCCATCGAAGCAGAACTCCTCACCGAGATCCGCGATCTCCTGCAGGAGCAAAAGCGCC
TTCAGTAG

Upstream 100 bases:

>100_bases
TTACACAACCGAAGAATAGTTCCCTATTCAAAAAAAGGTACTTTCCGCTACAGTTCATTTGTCGTTCTTACTGTCCACAC
TATCGTCAGGGGTAACAACC

Downstream 100 bases:

>100_bases
TTAAAAGGCCCTAAAAGCACAAAAGTGCGTCTGAGTATTTCACTCAGACGCACTTTTTGTTGCCTAAATTATGCTGCTAA
CCATGATGCGGTGGTTGCTG

Product: large conductance mechanosensitive channel

Products: non-specific ion/solute [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 135; Mature: 135

Protein sequence:

>135_residues
MLKGFRDFVLRGNVIELAVAVVIGTAFTAIVTAFSESIINPLIASIGSTEVEGLGFHIRAGNAATFVDFGAVITAAINFL
IIAAIVYFVLVAPMNKLSETLAKRKGVEEDETPASIEAELLTEIRDLLQEQKRLQ

Sequences:

>Translated_135_residues
MLKGFRDFVLRGNVIELAVAVVIGTAFTAIVTAFSESIINPLIASIGSTEVEGLGFHIRAGNAATFVDFGAVITAAINFL
IIAAIVYFVLVAPMNKLSETLAKRKGVEEDETPASIEAELLTEIRDLLQEQKRLQ
>Mature_135_residues
MLKGFRDFVLRGNVIELAVAVVIGTAFTAIVTAFSESIINPLIASIGSTEVEGLGFHIRAGNAATFVDFGAVITAAINFL
IIAAIVYFVLVAPMNKLSETLAKRKGVEEDETPASIEAELLTEIRDLLQEQKRLQ

Specific function: Channel that opens in response to stretch forces in the membrane lipid bilayer. May participate in the regulation of osmotic pressure changes within the cell [H]

COG id: COG1970

COG function: function code M; Large-conductance mechanosensitive channel

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mscL family [H]

Homologues:

Organism=Escherichia coli, GI1789686, Length=136, Percent_Identity=38.2352941176471, Blast_Score=74, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019823
- InterPro:   IPR001185
- InterPro:   IPR015089 [H]

Pfam domain/function: PF01741 MscL [H]

EC number: NA

Molecular weight: Translated: 14535; Mature: 14535

Theoretical pI: Translated: 4.55; Mature: 4.55

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLKGFRDFVLRGNVIELAVAVVIGTAFTAIVTAFSESIINPLIASIGSTEVEGLGFHIRA
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
GNAATFVDFGAVITAAINFLIIAAIVYFVLVAPMNKLSETLAKRKGVEEDETPASIEAEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LTEIRDLLQEQKRLQ
HHHHHHHHHHHHCCC
>Mature Secondary Structure
MLKGFRDFVLRGNVIELAVAVVIGTAFTAIVTAFSESIINPLIASIGSTEVEGLGFHIRA
CCCCHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
GNAATFVDFGAVITAAINFLIIAAIVYFVLVAPMNKLSETLAKRKGVEEDETPASIEAEL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHH
LTEIRDLLQEQKRLQ
HHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: non-specific ion/solute [Cytoplasm] [C]

Specific reaction: non-specific ion/solute [Cytoplasm] = non-specific ion/solute [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA