Click here to switch to the map view.

The map label for this gene is citE [H]

Identifier: 62389752

GI number: 62389752

Start: 923065

End: 923886

Strand: Direct

Name: citE [H]

Synonym: cg0985

Alternate gene names: 62389752

Gene position: 923065-923886 (Clockwise)

Preceding gene: 62389751

Following gene: 62389761

Centisome position: 28.12

GC content: 52.8

Gene sequence:

>822_bases
ATGTCTGAACTTATTTGTGGACCAGCTATTCTCTTCGCACCAGCTGGACGTGCTGAGATCATTCCAAAAGCAGCATCGAA
GGCCGATATGGTCATCATTGATTTGGAAGATGGGGCAGGGGAGGTAGACCGTGAGGTCGCCTACAGGAACATTAGAGAAT
CGGGGTTGGATCCTAAACGAACCATTGTGAGAACCGTAGGGCCGAGCGATCCACACTTTTTGGCTGACGTGGAGATGGTG
AAGTCCACGGATTTCACACTTGTTATGGTTCCTAAACTTCTTGGCAGCGTGCCTGAGGAATTAGATGGCCTCAACATTAT
CGCCATGATTGAAACCCCTCAGGCTGCAACCAGCATTCCTCAGATTGCTGCGGACCCTAAAGTCGTTGGAATGTTCTGGG
GCGCGGAGGATCTCACACACCTCTTGGGAGGCACTCATTCTAGGTTCTTGGGTGATGAGTCCAATGAAGGCTCCTACCGA
GACACCATGAGGCTTACACGCGCCCTGATGCACCTCCACGCGGCGGCGAATGGGAAGTTCACCATTGATGCCATCCATGC
GGATTTCCACGATGAAGAGGGCCTCTATTTAGAAGCGGTCGATGCTGCGCGGACTGGTTTCGCTGGCACCGCATGCATTC
ACCCCAAGCAGATCGAGATTGTTCGGAGAGCCTATCGGCCAGAGGCTAACCAGTTGGAGTGGGCGAAGAAAGTGGTGGAG
GAAGCAGAAAACCATCCAGGTGCGTTCAAACTGGATGGTCAGATGATTGATGCTCCGTTGATTTCGCAGGCGCGGATGGT
TATTTCGCGTCAGCCTGCTTGA

Upstream 100 bases:

>100_bases
ATGTACCTGACTGGTGCGCGACACTTCGCTCACTAAAGTTTTTAAAGATTTCGCTTGAAGGCAGACCATAAGGTCTGCCT
TTTCGCGTATTAATGAGTAC

Downstream 100 bases:

>100_bases
TTAGTTCAAGCGTTTTTTCGACCCGGTCGGCGGGCAGCGGCGCGCCGAGGACGGCGAGGGAGGCGTCGGCAAGCATAATT
GCGGTCTCCGGGAGGCTGTC

Product: citryl-COA lyase BETA subunit-like protein

Products: acetate; oxaloacetate

Alternate protein names: NA

Number of amino acids: Translated: 273; Mature: 272

Protein sequence:

>273_residues
MSELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKRTIVRTVGPSDPHFLADVEMV
KSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIPQIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYR
DTMRLTRALMHLHAAANGKFTIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIEIVRRAYRPEANQLEWAKKVVE
EAENHPGAFKLDGQMIDAPLISQARMVISRQPA

Sequences:

>Translated_273_residues
MSELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKRTIVRTVGPSDPHFLADVEMV
KSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIPQIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYR
DTMRLTRALMHLHAAANGKFTIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIEIVRRAYRPEANQLEWAKKVVE
EAENHPGAFKLDGQMIDAPLISQARMVISRQPA
>Mature_272_residues
SELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKRTIVRTVGPSDPHFLADVEMVK
STDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIPQIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYRD
TMRLTRALMHLHAAANGKFTIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIEIVRRAYRPEANQLEWAKKVVEE
AENHPGAFKLDGQMIDAPLISQARMVISRQPA

Specific function: May play a role in fatty acid biosynthesis (Potential) [H]

COG id: COG2301

COG function: function code G; Citrate lyase beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HpcH/HpaI aldolase family. Citrate lyase beta subunit-like subfamily [H]

Homologues:

Organism=Homo sapiens, GI45545437, Length=298, Percent_Identity=25.8389261744966, Blast_Score=88, Evalue=1e-17,
Organism=Escherichia coli, GI87081764, Length=288, Percent_Identity=26.7361111111111, Blast_Score=75, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI17557458, Length=304, Percent_Identity=25, Blast_Score=76, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005000
- InterPro:   IPR011206
- InterPro:   IPR015813 [H]

Pfam domain/function: PF03328 HpcH_HpaI [H]

EC number: 4.1.3.6

Molecular weight: Translated: 29731; Mature: 29600

Theoretical pI: Translated: 4.86; Mature: 4.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKR
CCCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHH
TIVRTVGPSDPHFLADVEMVKSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIP
HHEEECCCCCCCEEHHHHHHHCCCCEEEEHHHHHCCCHHHHCCCEEEEEEECCCHHHCCC
QIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYRDTMRLTRALMHLHAAANGKF
HHCCCCEEEEEEECHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
TIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIEIVRRAYRPEANQLEWAKKVVE
EEEEEECCCCCCCCCEEEEEHHHHCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHH
EAENHPGAFKLDGQMIDAPLISQARMVISRQPA
HHHCCCCEEEECCEEECCHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SELICGPAILFAPAGRAEIIPKAASKADMVIIDLEDGAGEVDREVAYRNIRESGLDPKR
CCCCCCCEEEEECCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCHHH
TIVRTVGPSDPHFLADVEMVKSTDFTLVMVPKLLGSVPEELDGLNIIAMIETPQAATSIP
HHEEECCCCCCCEEHHHHHHHCCCCEEEEHHHHHCCCHHHHCCCEEEEEEECCCHHHCCC
QIAADPKVVGMFWGAEDLTHLLGGTHSRFLGDESNEGSYRDTMRLTRALMHLHAAANGKF
HHCCCCEEEEEEECHHHHHHHHCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
TIDAIHADFHDEEGLYLEAVDAARTGFAGTACIHPKQIEIVRRAYRPEANQLEWAKKVVE
EEEEEECCCCCCCCCEEEEEHHHHCCCCCEEECCCHHHHHHHHHHCCCCHHHHHHHHHHH
EAENHPGAFKLDGQMIDAPLISQARMVISRQPA
HHHCCCCEEEECCEEECCHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 0.345 {acetyl-CoA}} 0.0718 {acetyl-CoA}} 0.0367 {oxaloacetate}} 0.0565 {oxaoacetate}} [C]

Substrates: citrate

Specific reaction: citrate = acetate + oxaloacetate

General reaction: elimination (of an oxo-acid C-C bond cleavage [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]