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The map label for this gene is thtR

Identifier: 62389590

GI number: 62389590

Start: 722017

End: 722922

Strand: Reverse

Name: thtR

Synonym: cg0803

Alternate gene names: 62389590

Gene position: 722922-722017 (Counterclockwise)

Preceding gene: 62389592

Following gene: 62389589

Centisome position: 22.02

GC content: 58.5

Gene sequence:

>906_bases
ATGGCAGCACCGTTCGACCCATTCCCAGCTTTTGAGGAATACGCACACCCTGAAAGGATCGTGTCTGCATCCTGGCTTTC
CGCCCGCCTCGGCTCCCCTGGTCTGAAGGTTGTGGAGTCCAATGAAGATTCCCTTCTTTACGATATCGGACACCTCCCCG
GCGCTGTGCGCATTGACTGGGCAAAGGACCTCAACGATCCCCTCACCCGCGATTTCATTGACGGTGAAGCTTTCGCTGAG
CTGATGAACCGCAAGGGCATCGCTCGCGATGACACCGTTGTTGTCTACGGTGACAAGTCCAACTGGTGGGCTGCGTTCAC
CCTGTGGGTCTTCGAACTGTTCGGCCACTCCGATGTCCGCCTGCTCAACGGCGGCCGCGACGCGTGGATGGCTGAAGAGC
GCGACACCTCCTACGTGGTTCCGGAGTACCCCTCCGCCAACTACCCCGTCGTGGAGCGTGTCGACGAAAACCAGCGCGCG
TTCGTGGCTGAGGTGCTCGGTTCGCTCACGCAATCCGGTGGCATGACGCTTGTCGACGTCAGGACCCCTTCGGAGTTCTC
CGGATTGGATGAGCACGGCAACCCAACCTCAAACACCGGCGTGCTTCGTGGTGGACACATCCCAGGCGCGATCAACCTGG
ATTGGTCGGACGCTGTTCTTCCCAACGGAAACTTCCGCACCCGTGCAGAGTTGGACAAGCTCTACGCCGATCTCAACCCA
GCTGACGATACCGTTGTCTACTGCCAGGTTGGCGACCGCGCGGCCCACACCTGGTTCGTGCTGAAGTATCTGCTCGGTTT
CAACAACGTCCGAAACTATGACGGATCGTGGGCAGAATGGGGCAATATGGTTCGCATGCCGATCGAAACTGGCGAAAACA
CCAAAAATAACGTTTCGGTGTCATAG

Upstream 100 bases:

>100_bases
CTAGTAATTAAGGACACAAACACCTTTAACCAATATGCTGGGATACAGTATTTAATTTTCACCCATTTATGCACTCGTTT
ATGGAAGGCAGCGACCCCAC

Downstream 100 bases:

>100_bases
AATAGGCGTATCCCCTTTTTGCCTGAACATAAGCCATATTTCCTGCCTAAAACAGAAGAATTCCCCCAGAATATGAGTAA
GTCCTCATATCTGGTTGAGT

Product: thiosulfate sulfurtransferase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 301; Mature: 300

Protein sequence:

>301_residues
MAAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDWAKDLNDPLTRDFIDGEAFAE
LMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVRLLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRA
FVAEVLGSLTQSGGMTLVDVRTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKNNVSVS

Sequences:

>Translated_301_residues
MAAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDWAKDLNDPLTRDFIDGEAFAE
LMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVRLLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRA
FVAEVLGSLTQSGGMTLVDVRTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKNNVSVS
>Mature_300_residues
AAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDWAKDLNDPLTRDFIDGEAFAEL
MNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVRLLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRAF
VAEVLGSLTQSGGMTLVDVRTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNPA
DDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKNNVSVS

Specific function: May Be A Sulfotransferase Involved In The Formation Of Thiosulfate. The Rhodanese Activity Of Ssea Is Weak, Its Participation In Detoxification Of Cyanide May Be Small. May Be Involved In The Enhancement Of Serine-Sensitivity. [C]

COG id: COG2897

COG function: function code P; Rhodanese-related sulfurtransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 rhodanese domains

Homologues:

Organism=Homo sapiens, GI17402865, Length=290, Percent_Identity=31.0344827586207, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI194473668, Length=274, Percent_Identity=28.1021897810219, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI61835204, Length=279, Percent_Identity=27.9569892473118, Blast_Score=87, Evalue=1e-17,
Organism=Homo sapiens, GI194473681, Length=279, Percent_Identity=27.9569892473118, Blast_Score=87, Evalue=1e-17,
Organism=Escherichia coli, GI87082121, Length=282, Percent_Identity=28.0141843971631, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI87081967, Length=300, Percent_Identity=28, Blast_Score=90, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17561888, Length=277, Percent_Identity=25.9927797833935, Blast_Score=75, Evalue=3e-14,
Organism=Caenorhabditis elegans, GI115534702, Length=226, Percent_Identity=29.2035398230088, Blast_Score=72, Evalue=3e-13,
Organism=Caenorhabditis elegans, GI71997283, Length=235, Percent_Identity=26.8085106382979, Blast_Score=70, Evalue=9e-13,
Organism=Saccharomyces cerevisiae, GI6324825, Length=240, Percent_Identity=27.5, Blast_Score=72, Evalue=7e-14,

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): THTR_CORGL (P71121)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927150
- EMBL:   U35023
- RefSeq:   NP_599933.1
- RefSeq:   YP_224992.1
- ProteinModelPortal:   P71121
- SMR:   P71121
- GeneID:   1018700
- GeneID:   3342624
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg0803
- KEGG:   cgl:NCgl0671
- HOGENOM:   HBG709927
- OMA:   EERDTSY
- PhylomeDB:   P71121
- ProtClustDB:   CLSK811394
- BioCyc:   CGLU196627:CG0803-MONOMER
- BRENDA:   2.8.1.1
- InterPro:   IPR001763
- InterPro:   IPR001307
- Gene3D:   G3DSA:3.40.250.10
- SMART:   SM00450

Pfam domain/function: PF00581 Rhodanese; SSF52821 Rhodanese-like

EC number: =2.8.1.1

Molecular weight: Translated: 33494; Mature: 33363

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS00380 RHODANESE_1; PS00683 RHODANESE_2; PS50206 RHODANESE_3

Important sites: ACT_SITE 248-248 BINDING 253-253

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDW
CCCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEEEEH
AKDLNDPLTRDFIDGEAFAELMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVR
HHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
LLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRAFVAEVLGSLTQSGGMTLVDV
EEECCCCCCEECCCCCCEECCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHCCCCEEEEE
RTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
CCCCHHCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKNNVSV
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCC
S
C
>Mature Secondary Structure 
AAPFDPFPAFEEYAHPERIVSASWLSARLGSPGLKVVESNEDSLLYDIGHLPGAVRIDW
CCCCCCCCCHHHHCCHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEECCCCCCEEEEEH
AKDLNDPLTRDFIDGEAFAELMNRKGIARDDTVVVYGDKSNWWAAFTLWVFELFGHSDVR
HHCCCCCHHHHHCCHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHHCCCCEE
LLNGGRDAWMAEERDTSYVVPEYPSANYPVVERVDENQRAFVAEVLGSLTQSGGMTLVDV
EEECCCCCCEECCCCCCEECCCCCCCCCCHHEECCCCHHHHHHHHHHHHHHCCCCEEEEE
RTPSEFSGLDEHGNPTSNTGVLRGGHIPGAINLDWSDAVLPNGNFRTRAELDKLYADLNP
CCCCHHCCCCCCCCCCCCCCEEECCCCCCEEECCCCCCCCCCCCCCHHHHHHHHHHCCCC
ADDTVVYCQVGDRAAHTWFVLKYLLGFNNVRNYDGSWAEWGNMVRMPIETGENTKNNVSV
CCCEEEEEEECCCHHHHHHHHHHHHCCCCCCCCCCCHHHCCCEEEEEECCCCCCCCCCCC
S
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12948626; 8772169