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The map label for this gene is pitA [H]

Identifier: 62389360

GI number: 62389360

Start: 481390

End: 482667

Strand: Reverse

Name: pitA [H]

Synonym: cg0545

Alternate gene names: 62389360

Gene position: 482667-481390 (Counterclockwise)

Preceding gene: 62389361

Following gene: 62389359

Centisome position: 14.7

GC content: 55.09

Gene sequence:

>1278_bases
GTGACCGAGCTGATTATTTTATTGATTGTTATCGTGACGGCGCTCGCCTTCGATTTCACAAACGGATTCCACGACACCGG
CAATGCGATGGCCACATCCATTGCCACAGGCGCTCTAAAACCTAAAGTCGCCGTGGCACTATCCGCCTCACTGAACCTTG
TTGGCGCATTCCTCTCTGTAGAAGTTGCGACAACTGTTGCCAAAGGCGTTGTTGACCTCGACCAATTCGACCTAAGCAAT
GCCTGGGATTCCCACCAGCTCCTGCTTGTCGTCTTCGCCGGCCTCATTGGCGCCATCGTCTGGAACCTTCTGACCTGGCT
GCTAGGCATTCCTTCCAGCTCCTCTCACGCACTTTTCGGTGGCCTCATTGGCGCCGCAATTGCTTCACTCGGTTTCGGCG
GAGTGGTGTGGGAAGGTGTCTTGTCCAAGATGATCATCCCAGCATTGGCTGCACCAGTTGTTGCAGGTCTCGTGGCCGCC
ATCGGCACTTTCGCCGTGTACAGCATCACAAAGGCAGTTGGAGACAACGAGAAGAACCGTTACTTCCGCTGGGGTCAGAT
CGGCTCCGCTTCCTTGGTTTCCCTGGCACACGGCACCAACGATGCCCAGAAGACCATGGGCGTTATCTTCCTTTCCCTGG
TTGCCACCGGTCACCTGGGAACTGACGCTGACATCCCATTCTGGGTCAAGGCTACATGTGCATTGGCAATCGCAATCGGT
ACCTACTTGGGTGGTTGGCGCGTTATCCGCACACTGGGCAAAGGCTTGGTTGAGATTGATTCCCCTCAGGGCATGGCAGC
AGAAACTTCTTCTGCAGCAATCATTTTGACTTCTTCCCACTTCGGTATGGCACTGTCCACCACTCACGTTGCTACTGGCT
CCATCATGGGTACCGGCATTGGACGTAAAGGGGCGAAGGTTCGTTGGTCCGTCGCAGGACGCATGGCAATGGCCTGGGTT
ATCACCCTCCCTGCCTCCGCGATCGTTGGCGTTTTCTGCTGGTGGGTAGCTCACGGAATTGGTCTTATCAGCTCAGACCT
CCTCGGAGTCCTCGTTGCATTCGCCATTCTGGTCATTCTGTCTGGCTACATTTACGCCCGTTCCCGTCGCGTGCCTGTTG
ATCCAAGCAACGTCAACGCTGACTGGAATGAAGAATCAAACAGCGTGGAACCTGCAACACCTTCCGCCCCGGCTGCTTCT
GAGATTACAGAAGCTCCTGCCGCTCCAGCCGCTCAAGCCGTTCAAGATCTCAACAACGAGAATGAGGTAACCAAGTAA

Upstream 100 bases:

>100_bases
CCCAATCATGGGGAATTCGAACTCTATCCTGGGCATTTACCGTCAGAAAATCCAAAATCGACATTTGGTTTCTACGCTTT
TTAGGGCATACTTCCCAATC

Downstream 100 bases:

>100_bases
TGAACTTCGCTACTATTTTCGGAAGCATCTTTGAGGTCACCCTCGTGGGCATTTTGCTCGGCGCAGGCCTTCCAGCCCTT
TTTGCATTAGGAATCCGCTT

Product: inorganic phosphate transporter

Products: Proton [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 425; Mature: 424

Protein sequence:

>425_residues
MTELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSASLNLVGAFLSVEVATTVAKGVVDLDQFDLSN
AWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFGGLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAA
IGTFAVYSITKAVGDNEKNRYFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGIGRKGAKVRWSVAGRMAMAWV
ITLPASAIVGVFCWWVAHGIGLISSDLLGVLVAFAILVILSGYIYARSRRVPVDPSNVNADWNEESNSVEPATPSAPAAS
EITEAPAAPAAQAVQDLNNENEVTK

Sequences:

>Translated_425_residues
MTELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSASLNLVGAFLSVEVATTVAKGVVDLDQFDLSN
AWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFGGLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAA
IGTFAVYSITKAVGDNEKNRYFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGIGRKGAKVRWSVAGRMAMAWV
ITLPASAIVGVFCWWVAHGIGLISSDLLGVLVAFAILVILSGYIYARSRRVPVDPSNVNADWNEESNSVEPATPSAPAAS
EITEAPAAPAAQAVQDLNNENEVTK
>Mature_424_residues
TELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSASLNLVGAFLSVEVATTVAKGVVDLDQFDLSNA
WDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFGGLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAAI
GTFAVYSITKAVGDNEKNRYFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIGT
YLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGIGRKGAKVRWSVAGRMAMAWVI
TLPASAIVGVFCWWVAHGIGLISSDLLGVLVAFAILVILSGYIYARSRRVPVDPSNVNADWNEESNSVEPATPSAPAASE
ITEAPAAPAAQAVQDLNNENEVTK

Specific function: Low-affinity inorganic phosphate transport [H]

COG id: COG0306

COG function: function code P; Phosphate/sulphate permeases

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the inorganic phosphate transporter (PiT) (TC 2.A.20) family. Pit subfamily [H]

Homologues:

Organism=Homo sapiens, GI31543630, Length=150, Percent_Identity=34, Blast_Score=87, Evalue=3e-17,
Organism=Homo sapiens, GI5803173, Length=149, Percent_Identity=30.8724832214765, Blast_Score=76, Evalue=5e-14,
Organism=Escherichia coli, GI1789360, Length=223, Percent_Identity=33.6322869955157, Blast_Score=98, Evalue=8e-22,
Organism=Escherichia coli, GI1789907, Length=224, Percent_Identity=34.375, Blast_Score=92, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71981576, Length=162, Percent_Identity=31.4814814814815, Blast_Score=85, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI25146401, Length=146, Percent_Identity=32.8767123287671, Blast_Score=81, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17539280, Length=157, Percent_Identity=28.0254777070064, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17536725, Length=159, Percent_Identity=27.6729559748428, Blast_Score=74, Evalue=2e-13,
Organism=Caenorhabditis elegans, GI32566716, Length=168, Percent_Identity=27.3809523809524, Blast_Score=73, Evalue=3e-13,
Organism=Saccharomyces cerevisiae, GI6319773, Length=158, Percent_Identity=27.8481012658228, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI21356511, Length=191, Percent_Identity=27.7486910994764, Blast_Score=73, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001204 [H]

Pfam domain/function: PF01384 PHO4 [H]

EC number: NA

Molecular weight: Translated: 43962; Mature: 43831

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSASLNLVGAFLSV
CHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
EVATTVAKGVVDLDQFDLSNAWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAAIGTFAVYSITKAVGDNEKNR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
EEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGI
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCCEEEEEHHHCCCCEEECCC
GRKGAKVRWSVAGRMAMAWVITLPASAIVGVFCWWVAHGIGLISSDLLGVLVAFAILVIL
CCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGYIYARSRRVPVDPSNVNADWNEESNSVEPATPSAPAASEITEAPAAPAAQAVQDLNNE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCC
NEVTK
CCCCC
>Mature Secondary Structure 
TELIILLIVIVTALAFDFTNGFHDTGNAMATSIATGALKPKVAVALSASLNLVGAFLSV
HHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCCCCEEEEEHHHHHHHHHHHHH
EVATTVAKGVVDLDQFDLSNAWDSHQLLLVVFAGLIGAIVWNLLTWLLGIPSSSSHALFG
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
GLIGAAIASLGFGGVVWEGVLSKMIIPALAAPVVAGLVAAIGTFAVYSITKAVGDNEKNR
HHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
YFRWGQIGSASLVSLAHGTNDAQKTMGVIFLSLVATGHLGTDADIPFWVKATCALAIAIG
EEECCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHH
TYLGGWRVIRTLGKGLVEIDSPQGMAAETSSAAIILTSSHFGMALSTTHVATGSIMGTGI
HHHHHHHHHHHHCCCCEEECCCCCCCCCCCCCEEEEEECCCCEEEEEHHHCCCCEEECCC
GRKGAKVRWSVAGRMAMAWVITLPASAIVGVFCWWVAHGIGLISSDLLGVLVAFAILVIL
CCCCCEEEEEHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SGYIYARSRRVPVDPSNVNADWNEESNSVEPATPSAPAASEITEAPAAPAAQAVQDLNNE
HHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHCCCCHHHHHHHHCCCC
NEVTK
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; phosphate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + phosphate [Periplasm] = Proton [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12788972 [H]