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The map label for this gene is emrB [C]

Identifier: 62389280

GI number: 62389280

Start: 402798

End: 404432

Strand: Direct

Name: emrB [C]

Synonym: cg0456

Alternate gene names: 62389280

Gene position: 402798-404432 (Clockwise)

Preceding gene: 62389279

Following gene: 62389282

Centisome position: 12.27

GC content: 55.72

Gene sequence:

>1635_bases
ATGACACACGAAACTTCCGTCCCCGGACCTGCCGACGCGCAGGTCGCAGGAGATACGAAGCTGCGCAAAGGCCGCGCGAA
GAAGGAAAAAACTCCTTCATCAATGACGCCTGAACAACAAAAGAAAGTCTGGTGGGTCCTCAGCGCGCTGATGGTCGCCA
TGATGATGGCCTCCCTTGACCAGATGATTTTCGGCACAGCCCTGCCAACAATCGTCGGTGAACTCGGCGGCGTTGACCAC
ATGATGTGGGTCATCACCGCATACCTACTTGCCGAAACCATCATGCTGCCGATCTACGGAAAGCTCGGCGACCTGGTTGG
ACGTAAAGGTCTCTTCATCGGAGCCCTCGGCATCTTCCTGATCGGCTCCGTCATCGGCGGGCTTGCAGGAAATATGACCT
GGTTGATCGTCGGCCGTGCCGTACAGGGCATCGGTGGCGGTGGACTGATGATCCTCTCGCAGGCAATCATCGCGGACGTT
GTTCCAGCACGTGAACGTGGCCGCTACATGGGTGTCATGGGTGGAGTCTTCGGACTCTCTGCAGTTCTTGGCCCACTACT
CGGTGGCTGGTTCACCGAAGGACCAGGCTGGCGCTGGGCATTCTGGATGAACATCCCACTGGGAATCATCGCCATCGGTG
TCGCCATTTACTTCCTGGACATTCCAAAGAAGAGCGTCAAGTTCCGCTGGGATTACCTGGGCACTTTCTTCATGATCGTT
GCCGCAACCAGCCTGATCCTGTTCACCACCTGGGGTGGATCCCAGTACGAGTGGTCTGATCCAATCATCATTGGACTGAT
CATCACCACCATCGTTGCCGCTGCACTGCTGGTTGTTGTGGAACTGCGCGCAAAAGATCCATTGGTTCCAATGTCCTTCT
TCCAAAACCGCAACTTCACGCTCACCACCATTGCAGGCCTGATCCTGGGTATCGCAATGTTCGGCATCATCGGCTACCTT
CCGACCTACCTCCAGATGGTCCACGGAATCAACGCCACCGAAGCCGGCTACATGCTGATCCCAATGATGGTCGGCATGAT
GGGTACCTCCATCTGGACTGGTATCCGCATCTCCAACACAGGAAAGTACAAACTCTTCCCACCAATCGGCATGGTGGTTA
CCTTCGTGGCACTGATCTTCTTTGCCCGAATGGAAGTGTCCACCACCCTGTGGCAGATCGGAATCTACCTCTTCGTCCTC
GGCGTCGGCCTGGGTCTAGCCATGCAGGTTCTGGTCCTGATCGTTCAGAACACCCTGCCAACCGCGGTGGTCGGATCCGC
AACCGCTGTGAACAACTTCTTCCGTCAAATCGGTTCCTCACTCGGATCCGCGCTGGTCGGTGGCATGTTCGTTGGCAACT
TGGGAACCCTCATGGAAGAAAGAATGCCAGCAGCCATGGCACAACTTTCACCAGAAGAACAAGCCGCCATGGCAGCCCAA
GGCGGACTGGACTCCAACGAATTGACGCCGGCAATCGTCAATCAATTGCCAACCGCGCTCCACGATGCGTTCGCCGGTTC
CTACAACGACGCACTCATCCCAGTGTTCTACGTGATGATGCCACTGATCGGCATCGCGCTGCTTCTCTTGCTGTTTATTA
AGCAAGAAAAACTACGCGAAACCACCACAGACTAA

Upstream 100 bases:

>100_bases
CCCTGACACCAATCTTCTTGGCGCTCACCCCGATCGCAGTAGTCGCCGCGATCCTCCTCTTTTTCATCCGTGAAGATCAC
CTCAAGGAAACGCACGAATA

Downstream 100 bases:

>100_bases
ACACAAAACAAATGAGACCTACCCTCGGGTAGGTCTCATTTGTTTAGGGTCGCGTCGAAAAGCAAAAAGCCTTAATCAAA
GACAACCGTGCGGTTACCGT

Product: major facilitator superfamily permease

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MTHETSVPGPADAQVAGDTKLRKGRAKKEKTPSSMTPEQQKKVWWVLSALMVAMMMASLDQMIFGTALPTIVGELGGVDH
MMWVITAYLLAETIMLPIYGKLGDLVGRKGLFIGALGIFLIGSVIGGLAGNMTWLIVGRAVQGIGGGGLMILSQAIIADV
VPARERGRYMGVMGGVFGLSAVLGPLLGGWFTEGPGWRWAFWMNIPLGIIAIGVAIYFLDIPKKSVKFRWDYLGTFFMIV
AATSLILFTTWGGSQYEWSDPIIIGLIITTIVAAALLVVVELRAKDPLVPMSFFQNRNFTLTTIAGLILGIAMFGIIGYL
PTYLQMVHGINATEAGYMLIPMMVGMMGTSIWTGIRISNTGKYKLFPPIGMVVTFVALIFFARMEVSTTLWQIGIYLFVL
GVGLGLAMQVLVLIVQNTLPTAVVGSATAVNNFFRQIGSSLGSALVGGMFVGNLGTLMEERMPAAMAQLSPEEQAAMAAQ
GGLDSNELTPAIVNQLPTALHDAFAGSYNDALIPVFYVMMPLIGIALLLLLFIKQEKLRETTTD

Sequences:

>Translated_544_residues
MTHETSVPGPADAQVAGDTKLRKGRAKKEKTPSSMTPEQQKKVWWVLSALMVAMMMASLDQMIFGTALPTIVGELGGVDH
MMWVITAYLLAETIMLPIYGKLGDLVGRKGLFIGALGIFLIGSVIGGLAGNMTWLIVGRAVQGIGGGGLMILSQAIIADV
VPARERGRYMGVMGGVFGLSAVLGPLLGGWFTEGPGWRWAFWMNIPLGIIAIGVAIYFLDIPKKSVKFRWDYLGTFFMIV
AATSLILFTTWGGSQYEWSDPIIIGLIITTIVAAALLVVVELRAKDPLVPMSFFQNRNFTLTTIAGLILGIAMFGIIGYL
PTYLQMVHGINATEAGYMLIPMMVGMMGTSIWTGIRISNTGKYKLFPPIGMVVTFVALIFFARMEVSTTLWQIGIYLFVL
GVGLGLAMQVLVLIVQNTLPTAVVGSATAVNNFFRQIGSSLGSALVGGMFVGNLGTLMEERMPAAMAQLSPEEQAAMAAQ
GGLDSNELTPAIVNQLPTALHDAFAGSYNDALIPVFYVMMPLIGIALLLLLFIKQEKLRETTTD
>Mature_543_residues
THETSVPGPADAQVAGDTKLRKGRAKKEKTPSSMTPEQQKKVWWVLSALMVAMMMASLDQMIFGTALPTIVGELGGVDHM
MWVITAYLLAETIMLPIYGKLGDLVGRKGLFIGALGIFLIGSVIGGLAGNMTWLIVGRAVQGIGGGGLMILSQAIIADVV
PARERGRYMGVMGGVFGLSAVLGPLLGGWFTEGPGWRWAFWMNIPLGIIAIGVAIYFLDIPKKSVKFRWDYLGTFFMIVA
ATSLILFTTWGGSQYEWSDPIIIGLIITTIVAAALLVVVELRAKDPLVPMSFFQNRNFTLTTIAGLILGIAMFGIIGYLP
TYLQMVHGINATEAGYMLIPMMVGMMGTSIWTGIRISNTGKYKLFPPIGMVVTFVALIFFARMEVSTTLWQIGIYLFVLG
VGLGLAMQVLVLIVQNTLPTAVVGSATAVNNFFRQIGSSLGSALVGGMFVGNLGTLMEERMPAAMAQLSPEEQAAMAAQG
GLDSNELTPAIVNQLPTALHDAFAGSYNDALIPVFYVMMPLIGIALLLLLFIKQEKLRETTTD

Specific function: Translocase That Confers Resistance To Substances Of High Hydrophobicity. [C]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=449, Percent_Identity=28.0623608017817, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI1788710, Length=483, Percent_Identity=27.9503105590062, Blast_Score=142, Evalue=7e-35,
Organism=Escherichia coli, GI87081983, Length=412, Percent_Identity=27.9126213592233, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI1788392, Length=319, Percent_Identity=32.2884012539185, Blast_Score=112, Evalue=6e-26,
Organism=Escherichia coli, GI1790195, Length=424, Percent_Identity=26.6509433962264, Blast_Score=105, Evalue=1e-23,
Organism=Escherichia coli, GI1790146, Length=204, Percent_Identity=26.9607843137255, Blast_Score=73, Evalue=5e-14,
Organism=Escherichia coli, GI87081738, Length=197, Percent_Identity=29.4416243654822, Blast_Score=68, Evalue=2e-12,
Organism=Saccharomyces cerevisiae, GI6322958, Length=493, Percent_Identity=26.1663286004057, Blast_Score=137, Evalue=6e-33,
Organism=Saccharomyces cerevisiae, GI6323735, Length=459, Percent_Identity=27.4509803921569, Blast_Score=134, Evalue=3e-32,
Organism=Saccharomyces cerevisiae, GI6319770, Length=421, Percent_Identity=27.0783847980998, Blast_Score=127, Evalue=4e-30,
Organism=Saccharomyces cerevisiae, GI6321663, Length=550, Percent_Identity=22.5454545454545, Blast_Score=115, Evalue=2e-26,
Organism=Saccharomyces cerevisiae, GI6319783, Length=399, Percent_Identity=24.5614035087719, Blast_Score=93, Evalue=1e-19,
Organism=Saccharomyces cerevisiae, GI6325455, Length=505, Percent_Identity=22.5742574257426, Blast_Score=92, Evalue=3e-19,
Organism=Saccharomyces cerevisiae, GI6324954, Length=453, Percent_Identity=24.9448123620309, Blast_Score=80, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6324264, Length=224, Percent_Identity=24.5535714285714, Blast_Score=64, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR005829
- InterPro:   IPR001958
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 58496; Mature: 58365

Theoretical pI: Translated: 8.61; Mature: 8.61

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
6.2 %Met     (Translated Protein)
6.2 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
6.1 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCCC
>Mature Secondary Structure 
THETSVPGPADAQVAGDTKLRKGRAKKEKTPSSMTPEQQKKVWWVLSALMVAMMMASLD
CCCCCCCCCCCCCCCCCHHHHHCCCHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHH
QMIFGTALPTIVGELGGVDHMMWVITAYLLAETIMLPIYGKLGDLVGRKGLFIGALGIFL
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHH
IGSVIGGLAGNMTWLIVGRAVQGIGGGGLMILSQAIIADVVPARERGRYMGVMGGVFGLS
HHHHHHHHHCCCEEEEEEHHHHCCCCCHHHHHHHHHHHHHCCHHHCCCCHHHHHHHHHHH
AVLGPLLGGWFTEGPGWRWAFWMNIPLGIIAIGVAIYFLDIPKKSVKFRWDYLGTFFMIV
HHHHHHHCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHH
AATSLILFTTWGGSQYEWSDPIIIGLIITTIVAAALLVVVELRAKDPLVPMSFFQNRNFT
HHHHHHEEEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCH
LTTIAGLILGIAMFGIIGYLPTYLQMVHGINATEAGYMLIPMMVGMMGTSIWTGIRISNT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEECCC
GKYKLFPPIGMVVTFVALIFFARMEVSTTLWQIGIYLFVLGVGLGLAMQVLVLIVQNTLP
CCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
TAVVGSATAVNNFFRQIGSSLGSALVGGMFVGNLGTLMEERMPAAMAQLSPEEQAAMAAQ
HHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
GGLDSNELTPAIVNQLPTALHDAFAGSYNDALIPVFYVMMPLIGIALLLLLFIKQEKLRE
CCCCCCCCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TTTD
CCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]