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The map label for this gene is yhcR [H]

Identifier: 62389226

GI number: 62389226

Start: 343637

End: 345721

Strand: Direct

Name: yhcR [H]

Synonym: cg0397

Alternate gene names: 62389226

Gene position: 343637-345721 (Clockwise)

Preceding gene: 62389225

Following gene: 62389234

Centisome position: 10.47

GC content: 56.02

Gene sequence:

>2085_bases
ATGAAGAGGCTTTCCCGTGCAGCCCTCGCAGTGGTCGCCACCACCGCAGTTAGCTTCAGCGCACTCGCAGTTCCAGCTTT
CGCAGACGAAGCAAGCAATGTTGAGCTCAACATCCTCGGTGTCACCGACTTCCACGGACACATCGAGCAGAAGGCTGTTA
AAGATGATAAGGGAGTAATCACCGGTTACTCAGAAATGGGTGCCAGTGGCGTTGCCTGCTACGTCGACGCTGAACGCGCG
GACAACCCAAACACCCGCTTCATCACCGTTGGTGACAACATTGGTGGATCCCCATTCGTGTCCTCCATCCTGAAGGATGA
GCCAACCTTGCAAGCCCTCAGCGCCATCGGTGTTGACGCATCCGCACTGGGCAATCACGAATTCGACCAGGGCTACTCAG
ACCTGGTGAACCGCGTTTCCCTCGACGGCTCCGGCAGCGCAAAGTTCCCATACCTCGGCGCAAACGTTGAAGGTGGCACC
CCAGCACCTGCAAAGTCTGAAATCATCGAGATGGACGGCGTCAAGATCGCTTACGTCGGCGCAGTAACCGAGGAGACCGC
AACCTTGGTCTCCCCAGCAGGCATCGAAGGCATCACCTTCACCGGCGACATCGACGCTATCAACGCAGAAGCAGATCGCG
TCATTGAGGCAGGCGAAGCAGACGTAGTCATCGCATTGATCCACGCTGAAGCCGCTCCAACCGATCTATTCTCCAACAAC
GTTGACGTTGTATTCTCCGGACACACCCACTTCGACTACGTTGCTGAAGGCGAAGCACGTGGCGACAAGCAGCCACTCGT
TGTCATCCAGGGCCACGAATACGGCAAGGTCATCTCCGACGTGGAGATCTCCTACGACCGCGAAGCAGGCAAGATCACCA
ACATTGAGGCGAAGAATGTCTCTGCTACTGACGTTGTGGAAAACTGTGAGACTCCAAACACAGCAGTCGACGCAATCGTT
GCAGCTGCTGTTGAGGCCGCTGAAGAAGCAGGTAATGAAGTTGTTGCAACCATTGACAACGGCTTCTACCGTGGGGCGGA
TGAAGAGGGTACGACCGGCTCCAACCGTGGTGTTGAGTCTTCCCTGAGCAACCTCATCGCAGAAGCTGGACTGTGGGCAG
TCAACGACGCGACCATCCTGAACGCTGACATCGGCATCATGAACGCAGGCGGCGTGCGTGCGGACCTCGAAGCAGGCGAA
GTTACCTTCGCAGATGCATACGCAACCCAGAACTTCTCCAACACCTACGGCGTACGTGAAGTGTCTGGTGCGCAGTTCAA
AGAAGCACTGGAACAGCAGTGGAAGGAAACCGGCGACCGCCCACGTCTGGCATTGGGACTGTCCAGCAACGTCCAGTACT
CCTACGACGAGACCCGCGAATACGGCGACCGCATCACCCACATCACCTTCAACGGTGAGCCAATGGATATGAAGGAGACC
TACCGCGTCACAGGATCATCCTTCCTGCTCGCAGGTGGCGACTCCTTCACTGCATTCGCTGAAGGCGGCCCAATCGCTGA
AACCGGCATGGTTGACATTGACCTGTTCAACAACTACATCGCAGCTCACCCAGATGCACCAATTCGTGCAAATCAGAGCT
CAGTAGGCATCGCCCTTTCCGGCCCGGCAGTTGCAGAAGACGGAACTTTGGTCCCTGGTGAAGAGCTGACCGTCGATCTT
TCTTCCCTCTCCTACACCGGACCTGAAGCTAAGCCAACCACCGTTGAGGTGACCGTTGGTACTGAGAAGAAGACTGCGGA
CGTCGATAACACCATCGTTCCTCAGTTTGACAGCACCGGCAAGGCAACTGTCACCCTGACTGTTCCTGAGGGAGCTACCT
CTGTCAAGATCGCAACTGACAATGGCACTACCTTTGAACTGCCAGTAACCGTAAACGGTGAAGGCAACAATGATGACGAT
GATGATAAGGAGCAGCAGTCCTCCGGATCCTCCGACGCCGGTTCCCTTGTAGCAGTTCTCGGTGTTCTTGGAGCACTCGG
TGGCCTGGTGGCGTTCTTCCTGAACTCTGCGCAGGGCGCACCATTCTTGGCTCAGCTTCAGGCTATGTTTGCGCAGTTCA
TGTAA

Upstream 100 bases:

>100_bases
AGCCCCAGCTCACCGAATTCTCCATTCGTTTTAATTGCTTCGTTAATTAAAACGCCATATAAAAACCGGCGCATTGCCGG
TATTTTTCCAGGAGAATTTA

Downstream 100 bases:

>100_bases
TAACTTGTAGTAAATAAATCGGGCCTTCCCAAAAGATTCTTTCTTGGGAAAGGCCCGATTTTGGTATTTGAGGTCTTTTA
GTGGAGTGTCTTAGACATTT

Product: 5'-nucleotidase precursor

Products: Phosphate; Uridine [C]

Alternate protein names: NA

Number of amino acids: Translated: 694; Mature: 694

Protein sequence:

>694_residues
MKRLSRAALAVVATTAVSFSALAVPAFADEASNVELNILGVTDFHGHIEQKAVKDDKGVITGYSEMGASGVACYVDAERA
DNPNTRFITVGDNIGGSPFVSSILKDEPTLQALSAIGVDASALGNHEFDQGYSDLVNRVSLDGSGSAKFPYLGANVEGGT
PAPAKSEIIEMDGVKIAYVGAVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALIHAEAAPTDLFSNN
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDVEISYDREAGKITNIEAKNVSATDVVENCETPNTAVDAIV
AAAVEAAEEAGNEVVATIDNGFYRGADEEGTTGSNRGVESSLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGE
VTFADAYATQNFSNTYGVREVSGAQFKEALEQQWKETGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALSGPAVAEDGTLVPGEELTVDL
SSLSYTGPEAKPTTVEVTVGTEKKTADVDNTIVPQFDSTGKATVTLTVPEGATSVKIATDNGTTFELPVTVNGEGNNDDD
DDKEQQSSGSSDAGSLVAVLGVLGALGGLVAFFLNSAQGAPFLAQLQAMFAQFM

Sequences:

>Translated_694_residues
MKRLSRAALAVVATTAVSFSALAVPAFADEASNVELNILGVTDFHGHIEQKAVKDDKGVITGYSEMGASGVACYVDAERA
DNPNTRFITVGDNIGGSPFVSSILKDEPTLQALSAIGVDASALGNHEFDQGYSDLVNRVSLDGSGSAKFPYLGANVEGGT
PAPAKSEIIEMDGVKIAYVGAVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALIHAEAAPTDLFSNN
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDVEISYDREAGKITNIEAKNVSATDVVENCETPNTAVDAIV
AAAVEAAEEAGNEVVATIDNGFYRGADEEGTTGSNRGVESSLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGE
VTFADAYATQNFSNTYGVREVSGAQFKEALEQQWKETGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALSGPAVAEDGTLVPGEELTVDL
SSLSYTGPEAKPTTVEVTVGTEKKTADVDNTIVPQFDSTGKATVTLTVPEGATSVKIATDNGTTFELPVTVNGEGNNDDD
DDKEQQSSGSSDAGSLVAVLGVLGALGGLVAFFLNSAQGAPFLAQLQAMFAQFM
>Mature_694_residues
MKRLSRAALAVVATTAVSFSALAVPAFADEASNVELNILGVTDFHGHIEQKAVKDDKGVITGYSEMGASGVACYVDAERA
DNPNTRFITVGDNIGGSPFVSSILKDEPTLQALSAIGVDASALGNHEFDQGYSDLVNRVSLDGSGSAKFPYLGANVEGGT
PAPAKSEIIEMDGVKIAYVGAVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALIHAEAAPTDLFSNN
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDVEISYDREAGKITNIEAKNVSATDVVENCETPNTAVDAIV
AAAVEAAEEAGNEVVATIDNGFYRGADEEGTTGSNRGVESSLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGE
VTFADAYATQNFSNTYGVREVSGAQFKEALEQQWKETGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALSGPAVAEDGTLVPGEELTVDL
SSLSYTGPEAKPTTVEVTVGTEKKTADVDNTIVPQFDSTGKATVTLTVPEGATSVKIATDNGTTFELPVTVNGEGNNDDD
DDKEQQSSGSSDAGSLVAVLGVLGALGGLVAFFLNSAQGAPFLAQLQAMFAQFM

Specific function: Sugar-nonspecific endonuclease that yields nucleotide 3'-monophosphate products. No 5'-nucleotidase activity was detected, using 5'-AMP as the substrate, in the presence of diverse divalent metals and with various pH values [H]

COG id: COG0737

COG function: function code F; 5'-nucleotidase/2',3'-cyclic phosphodiesterase and related esterases

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TNase-like domain [H]

Homologues:

Organism=Homo sapiens, GI4505467, Length=560, Percent_Identity=24.1071428571429, Blast_Score=120, Evalue=4e-27,
Organism=Escherichia coli, GI1786687, Length=574, Percent_Identity=24.5644599303136, Blast_Score=125, Evalue=1e-29,
Organism=Escherichia coli, GI1790658, Length=564, Percent_Identity=24.468085106383, Blast_Score=85, Evalue=1e-17,
Organism=Drosophila melanogaster, GI24641187, Length=479, Percent_Identity=24.8434237995825, Blast_Score=108, Evalue=2e-23,
Organism=Drosophila melanogaster, GI28573524, Length=575, Percent_Identity=24, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI19922444, Length=544, Percent_Identity=24.2647058823529, Blast_Score=87, Evalue=6e-17,
Organism=Drosophila melanogaster, GI24652512, Length=453, Percent_Identity=23.6203090507726, Blast_Score=84, Evalue=3e-16,
Organism=Drosophila melanogaster, GI19922446, Length=526, Percent_Identity=23.574144486692, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI24654424, Length=526, Percent_Identity=23.574144486692, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI19921980, Length=453, Percent_Identity=23.6203090507726, Blast_Score=84, Evalue=4e-16,
Organism=Drosophila melanogaster, GI161076508, Length=453, Percent_Identity=23.6203090507726, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: 60 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008334
- InterPro:   IPR006146
- InterPro:   IPR006179
- InterPro:   IPR019948
- InterPro:   IPR019931
- InterPro:   IPR004843
- InterPro:   IPR016027
- InterPro:   IPR004365
- InterPro:   IPR016071
- InterPro:   IPR006021 [H]

Pfam domain/function: PF02872 5_nucleotid_C; PF00746 Gram_pos_anchor; PF00149 Metallophos; PF00565 SNase; PF01336 tRNA_anti [H]

EC number: 3.6.1.45; 3.1.3.5 [C]

Molecular weight: Translated: 72288; Mature: 72288

Theoretical pI: Translated: 3.93; Mature: 3.93

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRLSRAALAVVATTAVSFSALAVPAFADEASNVELNILGVTDFHGHIEQKAVKDDKGVI
CCCHHHHHHHHHHHHHHHHHHEEEEHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCEE
TGYSEMGASGVACYVDAERADNPNTRFITVGDNIGGSPFVSSILKDEPTLQALSAIGVDA
EEHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCH
SALGNHEFDQGYSDLVNRVSLDGSGSAKFPYLGANVEGGTPAPAKSEIIEMDGVKIAYVG
HHCCCCCCCCCHHHHHHHEECCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEE
AVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALIHAEAAPTDLFSNN
EECCCHHHEECCCCCCCEEEECCCCEECCCHHHHEECCCCCEEEEEEECCCCCHHHHCCC
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDVEISYDREAGKITNIEAKNV
EEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCEEECEEEEECCCCCCEEEEECCCC
SATDVVENCETPNTAVDAIVAAAVEAAEEAGNEVVATIDNGFYRGADEEGTTGSNRGVES
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCCCCCHHH
SLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGEVTFADAYATQNFSNTYGVRE
HHHHHHHHCCCEEECCCEEEECCCEEEECCCCEEECCCCCEEEEHHHHHCCCCCCCCCEE
VSGAQFKEALEQQWKETGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEECHHHHHHHCCEEEEEEECCCCCCHHHH
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALS
EEECCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEEEC
GPAVAEDGTLVPGEELTVDLSSLSYTGPEAKPTTVEVTVGTEKKTADVDNTIVPQFDSTG
CCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
KATVTLTVPEGATSVKIATDNGTTFELPVTVNGEGNNDDDDDKEQQSSGSSDAGSLVAVL
CEEEEEEECCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHH
GVLGALGGLVAFFLNSAQGAPFLAQLQAMFAQFM
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MKRLSRAALAVVATTAVSFSALAVPAFADEASNVELNILGVTDFHGHIEQKAVKDDKGVI
CCCHHHHHHHHHHHHHHHHHHEEEEHHCCCCCCEEEEEEEEECCCCHHHHHHHCCCCCEE
TGYSEMGASGVACYVDAERADNPNTRFITVGDNIGGSPFVSSILKDEPTLQALSAIGVDA
EEHHHCCCCCEEEEEECCCCCCCCEEEEEECCCCCCCHHHHHHHCCCHHHHHHHHCCCCH
SALGNHEFDQGYSDLVNRVSLDGSGSAKFPYLGANVEGGTPAPAKSEIIEMDGVKIAYVG
HHCCCCCCCCCHHHHHHHEECCCCCCCCCCEECCCCCCCCCCCCCCCEEEECCEEEEEEE
AVTEETATLVSPAGIEGITFTGDIDAINAEADRVIEAGEADVVIALIHAEAAPTDLFSNN
EECCCHHHEECCCCCCCEEEECCCCEECCCHHHHEECCCCCEEEEEEECCCCCHHHHCCC
VDVVFSGHTHFDYVAEGEARGDKQPLVVIQGHEYGKVISDVEISYDREAGKITNIEAKNV
EEEEEECCCCEEEEECCCCCCCCCCEEEEECCCCCCEEECEEEEECCCCCCEEEEECCCC
SATDVVENCETPNTAVDAIVAAAVEAAEEAGNEVVATIDNGFYRGADEEGTTGSNRGVES
CHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCCEEEEEECCCEECCCCCCCCCCCCCCHHH
SLSNLIAEAGLWAVNDATILNADIGIMNAGGVRADLEAGEVTFADAYATQNFSNTYGVRE
HHHHHHHHCCCEEECCCEEEECCCEEEECCCCEEECCCCCEEEEHHHHHCCCCCCCCCEE
VSGAQFKEALEQQWKETGDRPRLALGLSSNVQYSYDETREYGDRITHITFNGEPMDMKET
CCCHHHHHHHHHHHHHCCCCCEEEEEECCCCEECHHHHHHHCCEEEEEEECCCCCCHHHH
YRVTGSSFLLAGGDSFTAFAEGGPIAETGMVDIDLFNNYIAAHPDAPIRANQSSVGIALS
EEECCCEEEEECCCCEEEEECCCCCCCCCEEEEEECCCEEEECCCCCCCCCCCCEEEEEC
GPAVAEDGTLVPGEELTVDLSSLSYTGPEAKPTTVEVTVGTEKKTADVDNTIVPQFDSTG
CCCCCCCCCCCCCCCEEEEHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
KATVTLTVPEGATSVKIATDNGTTFELPVTVNGEGNNDDDDDKEQQSSGSSDAGSLVAVL
CEEEEEEECCCCCEEEEEECCCCEEEEEEEECCCCCCCCCCHHHHHHCCCCCHHHHHHHH
GVLGALGGLVAFFLNSAQGAPFLAQLQAMFAQFM
HHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: H2O; UMP [C]

Specific reaction: H2O + UMP --> Phosphate + Uridine [C]

General reaction: Phosphorous acid anhydride hydrolysis [C]

Inhibitor: Inhibitor from Salmonella typhimurium cytoplasm [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8969498; 9384377 [H]