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The map label for this gene is dnaN [H]

Identifier: 62388895

GI number: 62388895

Start: 2292

End: 3476

Strand: Direct

Name: dnaN [H]

Synonym: cg0004

Alternate gene names: 62388895

Gene position: 2292-3476 (Clockwise)

Preceding gene: 62388893

Following gene: 62388896

Centisome position: 0.07

GC content: 56.88

Gene sequence:

>1185_bases
ATGGAGTCACAAAACGTGTCCTTCCGTGTGGCCAGGGAAGACCTGGTTACCGCGGTAGCCTGGGTCGCTCGCAACCTGCC
CACCAAACCGACTCAGCCGGTACTTCGAGCCATGCTGATCACTGCCGATGATGAGGGTCTTGAACTCGCCGGTTACGACT
ACGACGTATCCACCCGAGTTCGCCTCTCAGCTGAGGTTTCTCAGCCAGGTCGCATCGCAGTAGCCGGCAAGCTGCTGTCT
GAAATCACAGGATCTTTGCCCAACAAGCCCGTTGATTTCCGCATCGACGGATCCAAAGCTTTTGTCACTTGTGGTTCTTC
CCGCTTCGAGCTGCCACTGATCCCGCTTGATGATTACCCAATGCTGCCCAAGCTTCCTGCAGGTACCGGTTCCATCAACG
CCAAGCTGTTCACCGAAGCAGTCTCCCAGGTGGCATCCGCTGCAGGTAAAGATGATTCTTTGCCGATGCTCACCGGTGTC
AGCATGGAAATCGTGGGCAACCAGATCAACTTGGCTGCTACTGACCGTTTCCGCCTGGCGCTGCGCACCTTCGAATGGGA
AGCAAACGACCCAGAGCTCAACGTTAAACTGCTGATTCCTGCCCGCACCCTGCTGGACAACGCGCGTTCTTTGGATTCCG
GTCTCAACGATTCCATCGACATCGCTGTCGGTACCGGCGATCAGGTTGGCCGCGAAGGACTATTCGGCGTTCACACCGAT
AACCGCGAAACCACCACCAGGCTTCTCGACGCCGATTTCCCCAACATCGCACCACTGCTTCCCAAAGAGCACACCGCGAT
CGCATCAGTTGAAATCGCACCGCTGGTCGACGCCATCCGCCGCGTATCCCTGGTGGCAGAACGCAACGCTCAGATCGTCT
TGCACTTCAGCGAAGGCCAGGTCATCTTGACTGCAGGCGCAACCGAGGCAGGCCACGCGGAAGAAACCCTGCCGTGTGCA
TTCACCGGCAAGGAACTGACCATCGCGTTCAACCCTGGCTACCTGAAGGATGGACTTTCTGTAGTTCCAACTTCTCGAGC
AGTCTTCGGATTCACTGAGCCTTCCCGCCCAGCGATCATGATCCCAGAGCCTGAAGAAATGCCTTCCGCCAACGAAAATG
GCATTTTCCAAACTCCGGACACCTATTTCACCTACCTGCTCATGCCAGTGCGCCTGCCAGGCTAA

Upstream 100 bases:

>100_bases
GAACCTTCTTCCAAGAAGTTTTCCCTATCGGTAAACGTCCGGGTTTAGTTAAACGATGAACTATATTTGATCTATATTTC
CCAAGGAGCTTTAAAACAGC

Downstream 100 bases:

>100_bases
ACACAAAAGTTTCACCCTTTTCGCGCCTGACTTTGTACACTTTTCAACCGACAAAGTCAGGCGCGTTATCCAATCCACTT
CCCACCGACTAAAAGGAGGT

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 394; Mature: 394

Protein sequence:

>394_residues
MESQNVSFRVAREDLVTAVAWVARNLPTKPTQPVLRAMLITADDEGLELAGYDYDVSTRVRLSAEVSQPGRIAVAGKLLS
EITGSLPNKPVDFRIDGSKAFVTCGSSRFELPLIPLDDYPMLPKLPAGTGSINAKLFTEAVSQVASAAGKDDSLPMLTGV
SMEIVGNQINLAATDRFRLALRTFEWEANDPELNVKLLIPARTLLDNARSLDSGLNDSIDIAVGTGDQVGREGLFGVHTD
NRETTTRLLDADFPNIAPLLPKEHTAIASVEIAPLVDAIRRVSLVAERNAQIVLHFSEGQVILTAGATEAGHAEETLPCA
FTGKELTIAFNPGYLKDGLSVVPTSRAVFGFTEPSRPAIMIPEPEEMPSANENGIFQTPDTYFTYLLMPVRLPG

Sequences:

>Translated_394_residues
MESQNVSFRVAREDLVTAVAWVARNLPTKPTQPVLRAMLITADDEGLELAGYDYDVSTRVRLSAEVSQPGRIAVAGKLLS
EITGSLPNKPVDFRIDGSKAFVTCGSSRFELPLIPLDDYPMLPKLPAGTGSINAKLFTEAVSQVASAAGKDDSLPMLTGV
SMEIVGNQINLAATDRFRLALRTFEWEANDPELNVKLLIPARTLLDNARSLDSGLNDSIDIAVGTGDQVGREGLFGVHTD
NRETTTRLLDADFPNIAPLLPKEHTAIASVEIAPLVDAIRRVSLVAERNAQIVLHFSEGQVILTAGATEAGHAEETLPCA
FTGKELTIAFNPGYLKDGLSVVPTSRAVFGFTEPSRPAIMIPEPEEMPSANENGIFQTPDTYFTYLLMPVRLPG
>Mature_394_residues
MESQNVSFRVAREDLVTAVAWVARNLPTKPTQPVLRAMLITADDEGLELAGYDYDVSTRVRLSAEVSQPGRIAVAGKLLS
EITGSLPNKPVDFRIDGSKAFVTCGSSRFELPLIPLDDYPMLPKLPAGTGSINAKLFTEAVSQVASAAGKDDSLPMLTGV
SMEIVGNQINLAATDRFRLALRTFEWEANDPELNVKLLIPARTLLDNARSLDSGLNDSIDIAVGTGDQVGREGLFGVHTD
NRETTTRLLDADFPNIAPLLPKEHTAIASVEIAPLVDAIRRVSLVAERNAQIVLHFSEGQVILTAGATEAGHAEETLPCA
FTGKELTIAFNPGYLKDGLSVVPTSRAVFGFTEPSRPAIMIPEPEEMPSANENGIFQTPDTYFTYLLMPVRLPG

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=398, Percent_Identity=25.8793969849246, Blast_Score=106, Evalue=2e-24,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 42468; Mature: 42468

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MESQNVSFRVAREDLVTAVAWVARNLPTKPTQPVLRAMLITADDEGLELAGYDYDVSTRV
CCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHEEEEEECCCCCEEECCCCCCCEEE
RLSAEVSQPGRIAVAGKLLSEITGSLPNKPVDFRIDGSKAFVTCGSSRFELPLIPLDDYP
EEEEECCCCCEEEHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCC
MLPKLPAGTGSINAKLFTEAVSQVASAAGKDDSLPMLTGVSMEIVGNQINLAATDRFRLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEECCEEEEEECCEEEEE
LRTFEWEANDPELNVKLLIPARTLLDNARSLDSGLNDSIDIAVGTGDQVGREGLFGVHTD
EEEEEECCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECC
NRETTTRLLDADFPNIAPLLPKEHTAIASVEIAPLVDAIRRVSLVAERNAQIVLHFSEGQ
CCHHHHHEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCC
VILTAGATEAGHAEETLPCAFTGKELTIAFNPGYLKDGLSVVPTSRAVFGFTEPSRPAIM
EEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCEECCCCCEEEECCCCCCCEEE
IPEPEEMPSANENGIFQTPDTYFTYLLMPVRLPG
CCCCHHCCCCCCCCEEECCCCEEEEEEEEEECCC
>Mature Secondary Structure
MESQNVSFRVAREDLVTAVAWVARNLPTKPTQPVLRAMLITADDEGLELAGYDYDVSTRV
CCCCCEEEEEHHHHHHHHHHHHHHCCCCCCCHHHHHEEEEEECCCCCEEECCCCCCCEEE
RLSAEVSQPGRIAVAGKLLSEITGSLPNKPVDFRIDGSKAFVTCGSSRFELPLIPLDDYP
EEEEECCCCCEEEHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCCCEEEEECCCCCC
MLPKLPAGTGSINAKLFTEAVSQVASAAGKDDSLPMLTGVSMEIVGNQINLAATDRFRLA
CCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCEEECCEEEEECCEEEEEECCEEEEE
LRTFEWEANDPELNVKLLIPARTLLDNARSLDSGLNDSIDIAVGTGDQVGREGLFGVHTD
EEEEEECCCCCCEEEEEEECHHHHHHHHHHHHCCCCCCEEEEECCCCCCCCCCEEEEECC
NRETTTRLLDADFPNIAPLLPKEHTAIASVEIAPLVDAIRRVSLVAERNAQIVLHFSEGQ
CCHHHHHEECCCCCCCCCCCCCCCCEEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCC
VILTAGATEAGHAEETLPCAFTGKELTIAFNPGYLKDGLSVVPTSRAVFGFTEPSRPAIM
EEEEECCCCCCCCCCCCCEEECCCEEEEEECCCCCCCCCEECCCCCEEEECCCCCCCEEE
IPEPEEMPSANENGIFQTPDTYFTYLLMPVRLPG
CCCCHHCCCCCCCCEEECCCCEEEEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8733228 [H]