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The map label for this gene is ybiN

Identifier: 59714336

GI number: 59714336

Start: 1295795

End: 1296754

Strand: Reverse

Name: ybiN

Synonym: VF_A1153

Alternate gene names: 59714336

Gene position: 1296754-1295795 (Counterclockwise)

Preceding gene: 59714337

Following gene: 172087825

Centisome position: 97.48

GC content: 37.81

Gene sequence:

>960_bases
ATGACAAATACTAAATCAAACGTTACTAAGAAATCATTGCACCCACGTAATAAACACACGGGAAACTATGATTTTCCAGC
TTTGATTAAGGCGTGTGCAGAATTAAAACCGTTTGTTGAGACCAATCAATACGGTAATGAATCGATTAACTTTTCAGATC
CTCAAGCGGTTAAGGCGTTAAATAAAGCGTTATTAGCGCATTTCTACAATGTACCGTTTTGGGATATTCCGCAGGGCTAC
CTGTGTCCACCTATTCCGGGACGTGCTGATTACATTCATAATATTGCTGATTTACTTGCTATTACTAATGAAGGGACAAT
CCCAACAGGTAAGAAAGTAAAAGGTTTAGATGTGGGTGTGGGTGCAAACTGTGTTTACCTAATTATTGGTAATCGTGAAT
ATCAGTGGAGTTTTGTTGGCTCAGACATTGATCCTCAATCGATTAAAATGGCTTCGTTCATTGCTAATAATAACCCAAGC
CTAAAAGGCATTGAGTGTCGTCTGCAAAAGAATGAAGAGAACATTTTTAAAGGTATTATCAAGCCAACTGAGTTTTTTGA
CTTTACTATGTGTAATCCTCCTTTCCATGCATCAGAAGCAGAAGCGACAGCAGGAACTGAGCGTAAGCAAAAAAATCTAG
CAGCAAATAAAGCGAAAAAAGGCCACGCTTTCCAAGAGATGCAGAATGCAAAAGCATTGAACTTTGGTGGTCAAAAAGCG
GAATTGTGGTGTGAAGGTGGTGAATTAGCCTTTATTCTTAAAATGGCTCAGCAAAGTCGTGAATTTTCACTACAAGTACA
ATGGTTTACTACATTGATTTCTAAGAAAGAAAACGTAGCTGAACTGTATAAAGAATTAGAAAAGATCGGCGTTAAAACGA
TTAAAACCATTGAAATGGCTCAAGGGCAAAAAATCAGTCGTTTTGTGGCTTGGACTTACCAGGCAAATGTAAATTTGTAA

Upstream 100 bases:

>100_bases
CTCAGTTTAGAAGGCATCGGGTGTCAGTTCACTCGATGCCTTTTTCTTTTTTTCGCTATTTCTGATCACAGATTGTGATT
GGTATTACTCGTAAGTTGAT

Downstream 100 bases:

>100_bases
GCTTTTAGATTTGATTGTTACTTGTTGATAATAAAGAATTTATTAATTATTTTATTATCAATAAATATCAACGTAAATTA
CAGACACATTCAGTAACATA

Product: putative SAM-dependent methyltransferase

Products: NA

Alternate protein names: 23S rRNA mA1618 methyltransferase; rRNA adenine N-6-methyltransferase

Number of amino acids: Translated: 319; Mature: 318

Protein sequence:

>319_residues
MTNTKSNVTKKSLHPRNKHTGNYDFPALIKACAELKPFVETNQYGNESINFSDPQAVKALNKALLAHFYNVPFWDIPQGY
LCPPIPGRADYIHNIADLLAITNEGTIPTGKKVKGLDVGVGANCVYLIIGNREYQWSFVGSDIDPQSIKMASFIANNNPS
LKGIECRLQKNEENIFKGIIKPTEFFDFTMCNPPFHASEAEATAGTERKQKNLAANKAKKGHAFQEMQNAKALNFGGQKA
ELWCEGGELAFILKMAQQSREFSLQVQWFTTLISKKENVAELYKELEKIGVKTIKTIEMAQGQKISRFVAWTYQANVNL

Sequences:

>Translated_319_residues
MTNTKSNVTKKSLHPRNKHTGNYDFPALIKACAELKPFVETNQYGNESINFSDPQAVKALNKALLAHFYNVPFWDIPQGY
LCPPIPGRADYIHNIADLLAITNEGTIPTGKKVKGLDVGVGANCVYLIIGNREYQWSFVGSDIDPQSIKMASFIANNNPS
LKGIECRLQKNEENIFKGIIKPTEFFDFTMCNPPFHASEAEATAGTERKQKNLAANKAKKGHAFQEMQNAKALNFGGQKA
ELWCEGGELAFILKMAQQSREFSLQVQWFTTLISKKENVAELYKELEKIGVKTIKTIEMAQGQKISRFVAWTYQANVNL
>Mature_318_residues
TNTKSNVTKKSLHPRNKHTGNYDFPALIKACAELKPFVETNQYGNESINFSDPQAVKALNKALLAHFYNVPFWDIPQGYL
CPPIPGRADYIHNIADLLAITNEGTIPTGKKVKGLDVGVGANCVYLIIGNREYQWSFVGSDIDPQSIKMASFIANNNPSL
KGIECRLQKNEENIFKGIIKPTEFFDFTMCNPPFHASEAEATAGTERKQKNLAANKAKKGHAFQEMQNAKALNFGGQKAE
LWCEGGELAFILKMAQQSREFSLQVQWFTTLISKKENVAELYKELEKIGVKTIKTIEMAQGQKISRFVAWTYQANVNL

Specific function: Specifically methylates the adenine in position 1618 of 23S rRNA

COG id: COG3129

COG function: function code R; Predicted SAM-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential)

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. METT10D/rlmF family

Homologues:

Organism=Homo sapiens, GI122114654, Length=313, Percent_Identity=28.4345047923323, Blast_Score=138, Evalue=5e-33,
Organism=Escherichia coli, GI87081786, Length=305, Percent_Identity=51.8032786885246, Blast_Score=324, Evalue=4e-90,
Organism=Caenorhabditis elegans, GI32564931, Length=314, Percent_Identity=27.7070063694268, Blast_Score=130, Evalue=8e-31,
Organism=Caenorhabditis elegans, GI32564933, Length=314, Percent_Identity=25.4777070063694, Blast_Score=105, Evalue=3e-23,
Organism=Drosophila melanogaster, GI281363415, Length=320, Percent_Identity=26.875, Blast_Score=127, Evalue=7e-30,
Organism=Drosophila melanogaster, GI19922302, Length=320, Percent_Identity=26.875, Blast_Score=127, Evalue=7e-30,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): RLMF_VIBF1 (Q5DYC3)

Other databases:

- EMBL:   CP000021
- RefSeq:   YP_207111.1
- ProteinModelPortal:   Q5DYC3
- STRING:   Q5DYC3
- GeneID:   3280574
- GenomeReviews:   CP000021_GR
- KEGG:   vfi:VF_A1153
- NMPDR:   fig|312309.3.peg.3142
- eggNOG:   COG3129
- HOGENOM:   HBG747880
- OMA:   AELWCEG
- PhylomeDB:   Q5DYC3
- ProtClustDB:   PRK11727
- BioCyc:   VFIS312309:VFA1153-MONOMER
- GO:   GO:0005737
- HAMAP:   MF_01848
- InterPro:   IPR016909
- InterPro:   IPR010286
- PANTHER:   PTHR13393
- PIRSF:   PIRSF029038

Pfam domain/function: PF05971 Methyltransf_10

EC number: =2.1.1.181

Molecular weight: Translated: 35702; Mature: 35571

Theoretical pI: Translated: 9.11; Mature: 9.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNTKSNVTKKSLHPRNKHTGNYDFPALIKACAELKPFVETNQYGNESINFSDPQAVKAL
CCCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHH
NKALLAHFYNVPFWDIPQGYLCPPIPGRADYIHNIADLLAITNEGTIPTGKKVKGLDVGV
HHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECC
GANCVYLIIGNREYQWSFVGSDIDPQSIKMASFIANNNPSLKGIECRLQKNEENIFKGII
CCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHC
KPTEFFDFTMCNPPFHASEAEATAGTERKQKNLAANKAKKGHAFQEMQNAKALNFGGQKA
CCHHHEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCEECCCCCEE
ELWCEGGELAFILKMAQQSREFSLQVQWFTTLISKKENVAELYKELEKIGVKTIKTIEMA
EEEECCCCEEEHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
QGQKISRFVAWTYQANVNL
CCCCEEEEEEEEEEECCCC
>Mature Secondary Structure 
TNTKSNVTKKSLHPRNKHTGNYDFPALIKACAELKPFVETNQYGNESINFSDPQAVKAL
CCCCCCCHHHHCCCCCCCCCCCCHHHHHHHHHHHCCCHHCCCCCCCCCCCCCHHHHHHH
NKALLAHFYNVPFWDIPQGYLCPPIPGRADYIHNIADLLAITNEGTIPTGKKVKGLDVGV
HHHHHHHHHCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECC
GANCVYLIIGNREYQWSFVGSDIDPQSIKMASFIANNNPSLKGIECRLQKNEENIFKGII
CCCEEEEEECCCEEEEEECCCCCCHHHHHHHHHHCCCCCCCCEEEEEEECCHHHHHHHHC
KPTEFFDFTMCNPPFHASEAEATAGTERKQKNLAANKAKKGHAFQEMQNAKALNFGGQKA
CCHHHEEEEECCCCCCCCCCHHHCCCHHHHHHHHHHCCCCCHHHHHHHCCCEECCCCCEE
ELWCEGGELAFILKMAQQSREFSLQVQWFTTLISKKENVAELYKELEKIGVKTIKTIEMA
EEEECCCCEEEHHHHHHCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHCHHHHHHHHHH
QGQKISRFVAWTYQANVNL
CCCCEEEEEEEEEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA