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The map label for this gene is rpoQ [H]

Identifier: 59714198

GI number: 59714198

Start: 1140896

End: 1141780

Strand: Reverse

Name: rpoQ [H]

Synonym: VF_A1015

Alternate gene names: 59714198

Gene position: 1141780-1140896 (Counterclockwise)

Preceding gene: 59714201

Following gene: 59714197

Centisome position: 85.83

GC content: 32.43

Gene sequence:

>885_bases
ATGGCTTATGCAAAGTTAGATATGAGAGTCTGCGAAGAACAGGGTTTAAACGATTCATATTCTTTATATTTAAAGGAAAT
AAATCGCTATCAGTTACTTACTGCAGAAGAGGAGTTGGCCTATAGTCGACTTTATAAAAAAGGGGATGTTTCAGCAAGGA
ATACATTAATTGAATCAAATCTGAGGCTTGTTGTTAAAGTTGCGAATAAATATCGAAAAAGAAACCAGACTGATTTAGCA
ATTTTAGACATTATTGAAGAAGGTAATTTGGGATTAATCAAAGCAATTGATAAATTTAATCCCGAATTAGGGTATCGCTT
TTCAACTTATGCTGTTTGGTGGATTCGAGAATCCATTGAAAGTGCGTTGTTTAATCATTCTAGAACTGTTCGTCTTCCTG
TCCATATAACTAAAGAGCTAAATACTTACCTTAGAGCAGCTCGTGAGTTATCTAAATCATTAAAACGTGAACCTTCTATT
AGAGACATTTCGACTTACTGTGAAGAAGAACAAATTAAAGTAAGTAAGATTATAAGTTTGATACCTAACCAACTGACTTG
CCATTCTGCATGTAGTGATGAGTTGTCTCCGAAGTTTTTTGAACTTTCATCAAATAATAAAGCATTAGAGCCTGATAGTT
CTTTATCTCAAAATAACTTAGAAATGAATTTGTCGTCTTGGATTGATTCTTTAAATTACAGAGAGAAAGAGATCATCATC
AATCGCTATGGGTTGTTTGGATGTAAAGTTAAGACATTAGAGGATTTAGGTAAAGATCTGGATTTATCAAAAGAAAGAGT
TCGACAAATACAATCTGAAACACTCATTAAGTTGAATACTATTTTTAAGAAAAACAAATTGGATTTAGAAATAGCACTGG
GTTGA

Upstream 100 bases:

>100_bases
TTAGTCTTTATACCATAGTTTATTTACAACTGAAAAACAGTTGATAACAATAAATATAAAATAGCTAGAGATATAAAACA
GCTAGGTACAAGGATATGTT

Downstream 100 bases:

>100_bases
TATTGTTTCATATTTAATGTCATGGATATGTTGAGGTTGTATTTATATGGATGATGGCGTTATTTTAAAATTGTTGTTGT
TTGCTAATGTTGTTTCTTTA

Product: sigma-Q factor RpoQ, quorum-sensing regulated RpoS-like sigma subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 293

Protein sequence:

>294_residues
MAYAKLDMRVCEEQGLNDSYSLYLKEINRYQLLTAEEELAYSRLYKKGDVSARNTLIESNLRLVVKVANKYRKRNQTDLA
ILDIIEEGNLGLIKAIDKFNPELGYRFSTYAVWWIRESIESALFNHSRTVRLPVHITKELNTYLRAARELSKSLKREPSI
RDISTYCEEEQIKVSKIISLIPNQLTCHSACSDELSPKFFELSSNNKALEPDSSLSQNNLEMNLSSWIDSLNYREKEIII
NRYGLFGCKVKTLEDLGKDLDLSKERVRQIQSETLIKLNTIFKKNKLDLEIALG

Sequences:

>Translated_294_residues
MAYAKLDMRVCEEQGLNDSYSLYLKEINRYQLLTAEEELAYSRLYKKGDVSARNTLIESNLRLVVKVANKYRKRNQTDLA
ILDIIEEGNLGLIKAIDKFNPELGYRFSTYAVWWIRESIESALFNHSRTVRLPVHITKELNTYLRAARELSKSLKREPSI
RDISTYCEEEQIKVSKIISLIPNQLTCHSACSDELSPKFFELSSNNKALEPDSSLSQNNLEMNLSSWIDSLNYREKEIII
NRYGLFGCKVKTLEDLGKDLDLSKERVRQIQSETLIKLNTIFKKNKLDLEIALG
>Mature_293_residues
AYAKLDMRVCEEQGLNDSYSLYLKEINRYQLLTAEEELAYSRLYKKGDVSARNTLIESNLRLVVKVANKYRKRNQTDLAI
LDIIEEGNLGLIKAIDKFNPELGYRFSTYAVWWIRESIESALFNHSRTVRLPVHITKELNTYLRAARELSKSLKREPSIR
DISTYCEEEQIKVSKIISLIPNQLTCHSACSDELSPKFFELSSNNKALEPDSSLSQNNLEMNLSSWIDSLNYREKEIIIN
RYGLFGCKVKTLEDLGKDLDLSKERVRQIQSETLIKLNTIFKKNKLDLEIALG

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is involved in acid resistance [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1789098, Length=273, Percent_Identity=43.956043956044, Blast_Score=250, Evalue=8e-68,
Organism=Escherichia coli, GI1789448, Length=258, Percent_Identity=31.7829457364341, Blast_Score=153, Evalue=1e-38,
Organism=Escherichia coli, GI1789871, Length=260, Percent_Identity=25, Blast_Score=90, Evalue=2e-19,

Paralogues:

None

Copy number: <10 (log phase) 250 (stationary phase) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012761
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 34014; Mature: 33883

Theoretical pI: Translated: 8.17; Mature: 8.17

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAYAKLDMRVCEEQGLNDSYSLYLKEINRYQLLTAEEELAYSRLYKKGDVSARNTLIESN
CCCHHHHHHHHHHCCCCCHHHHHHHHCCHHEEEECHHHHHHHHHHHCCCCHHHHHHHHCC
LRLVVKVANKYRKRNQTDLAILDIIEEGNLGLIKAIDKFNPELGYRFSTYAVWWIRESIE
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHH
SALFNHSRTVRLPVHITKELNTYLRAARELSKSLKREPSIRDISTYCEEEQIKVSKIISL
HHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IPNQLTCHSACSDELSPKFFELSSNNKALEPDSSLSQNNLEMNLSSWIDSLNYREKEIII
CCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCHHEEE
NRYGLFGCKVKTLEDLGKDLDLSKERVRQIQSETLIKLNTIFKKNKLDLEIALG
EECCCCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
>Mature Secondary Structure 
AYAKLDMRVCEEQGLNDSYSLYLKEINRYQLLTAEEELAYSRLYKKGDVSARNTLIESN
CCHHHHHHHHHHCCCCCHHHHHHHHCCHHEEEECHHHHHHHHHHHCCCCHHHHHHHHCC
LRLVVKVANKYRKRNQTDLAILDIIEEGNLGLIKAIDKFNPELGYRFSTYAVWWIRESIE
HHHHHHHHHHHHHCCCCCEEEEEECCCCCCHHHHHHHHCCCCCCCEEHHHHHHHHHHHHH
SALFNHSRTVRLPVHITKELNTYLRAARELSKSLKREPSIRDISTYCEEEQIKVSKIISL
HHHHCCCCEEEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHH
IPNQLTCHSACSDELSPKFFELSSNNKALEPDSSLSQNNLEMNLSSWIDSLNYREKEIII
CCCCCCHHHHCCCCCCCCEEEECCCCCCCCCCCCCCCCCCEEEHHHHHHHCCCCCHHEEE
NRYGLFGCKVKTLEDLGKDLDLSKERVRQIQSETLIKLNTIFKKNKLDLEIALG
EECCCCCEEEHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7959068; 10984043 [H]