Click here to switch to the map view.

The map label for this gene is fruB [H]

Identifier: 59714125

GI number: 59714125

Start: 1063465

End: 1064601

Strand: Reverse

Name: fruB [H]

Synonym: VF_A0942

Alternate gene names: 59714125

Gene position: 1064601-1063465 (Counterclockwise)

Preceding gene: 59714127

Following gene: 59714124

Centisome position: 80.03

GC content: 41.78

Gene sequence:

>1137_bases
ATGCTGACATTAACAAAGAATGACATTACGCTGCAGCAGTCAGCAGCGGATAAGACAACAGCAATAAAAGCAATTGCAAA
ACAGTTAGCAACCAAAGGATTAGTTGCAGAAAATTATGTAGATGGAATGCTTAATCGTGAGCAACAAAACTCAACGTTTT
TAGGTAACGGCATTGCGATTCCACATGGCACGGTTGATACCCGTGAACTGGTTAATCAGACGGGGGTCGCGATTCATCAT
TTTCCACAAGGCGTAAATTGGGGTGATGATAATGTGGTTTATGTGGCGATCGGTATTGCGGCTAAATCAGATGAGCACTT
AGGTATTCTTAAGCAATTAACGAAAGTGTTGTCAGCCGATGGTGTAGAAGAGAAGCTAAAACAAGCACAAACCGAAGCTG
ACATTATTGCATTATTAAATGGCGAAGTTCAGTTTGAAGCGGATTTTGATGCGTCACTTATTCAATTACTTTTCCCTGCA
AGTGACATGATCCAAATGAGTGCGGTTGCTGGCGGTTTATTAAAAAATAGCGGTAACGCAGAGAGCGCATTTGTTGCTGA
ACTGGTAACTAAAGAACCGACTCATTTAGGCAAAGGCTTATGGTTAGTAAGTACGGACAAAGGCGTAAAACGCTCAGGCA
TGTCTATTGTTACCACGGCAAATGGTTGTGAGTTTAATGAATTACCAGTGAAAGGTCTGATTGCGATTGCTTCTTGTAAT
GCTTCTCATCAAGCGTTTTTAACGACGATCACTAAGGCGATTACTGAGCAACAACAAGAGTCGATTCTATCTGCAACTTC
TGAACAGTTACTTGCCATGTTCTCTACTTCTACTGAAGAAACAACAGTAAATGCTGATGCGGATAACACTGCAATCTTTA
AAATTAAAAATGCGCACGGTTTACACGCTCGCCCGGGTGCAATGCTTGTTGCTGAAGCGAAAAAATACGAATCAAAAATC
ACCGTATTAAACGTAAATGGTGATGGTAACGCAGTGAATGCGAAAAGTTTAATGAAAGTGATCGCTCTTGGTGTTAAACA
CGGCCATGAACTGCAATTTACCGCAGATGGTGCTGATGCAAAAGAAGCGCTTGCTGGTATTGGTGCTGCGATTGCTTCTG
GTTTAGGCGAGGGTTAA

Upstream 100 bases:

>100_bases
AACTTTTTTTGAGTGCTTTGCTGAATCGATTCAGTTTGTTTTGCAAGGCAATAAATACGATTGATTATCAGTGAACATAG
ATAGCGAGAGGCTAAGACCC

Downstream 100 bases:

>100_bases
TTGATGAGTACTCATTTAAAAAGCAAAGTCGTAACTATCACCCTGAACCCTGCTTTAGATTTAACCGGTAGTTTAGATGC
GTTATCAGTTGGTTCTGTGA

Product: bifunctional PTS system fructose-specific transporter subunit IIA/HPr protein

Products: protein histidine; sugar phosphate

Alternate protein names: MTP; Phosphotransferase FPr protein; Pseudo-HPr; Phosphocarrier protein HPr; Protein H; Fructose-specific phosphotransferase enzyme IIA component; PTS system fructose-specific EIIA component [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLTLTKNDITLQQSAADKTTAIKAIAKQLATKGLVAENYVDGMLNREQQNSTFLGNGIAIPHGTVDTRELVNQTGVAIHH
FPQGVNWGDDNVVYVAIGIAAKSDEHLGILKQLTKVLSADGVEEKLKQAQTEADIIALLNGEVQFEADFDASLIQLLFPA
SDMIQMSAVAGGLLKNSGNAESAFVAELVTKEPTHLGKGLWLVSTDKGVKRSGMSIVTTANGCEFNELPVKGLIAIASCN
ASHQAFLTTITKAITEQQQESILSATSEQLLAMFSTSTEETTVNADADNTAIFKIKNAHGLHARPGAMLVAEAKKYESKI
TVLNVNGDGNAVNAKSLMKVIALGVKHGHELQFTADGADAKEALAGIGAAIASGLGEG

Sequences:

>Translated_378_residues
MLTLTKNDITLQQSAADKTTAIKAIAKQLATKGLVAENYVDGMLNREQQNSTFLGNGIAIPHGTVDTRELVNQTGVAIHH
FPQGVNWGDDNVVYVAIGIAAKSDEHLGILKQLTKVLSADGVEEKLKQAQTEADIIALLNGEVQFEADFDASLIQLLFPA
SDMIQMSAVAGGLLKNSGNAESAFVAELVTKEPTHLGKGLWLVSTDKGVKRSGMSIVTTANGCEFNELPVKGLIAIASCN
ASHQAFLTTITKAITEQQQESILSATSEQLLAMFSTSTEETTVNADADNTAIFKIKNAHGLHARPGAMLVAEAKKYESKI
TVLNVNGDGNAVNAKSLMKVIALGVKHGHELQFTADGADAKEALAGIGAAIASGLGEG
>Mature_378_residues
MLTLTKNDITLQQSAADKTTAIKAIAKQLATKGLVAENYVDGMLNREQQNSTFLGNGIAIPHGTVDTRELVNQTGVAIHH
FPQGVNWGDDNVVYVAIGIAAKSDEHLGILKQLTKVLSADGVEEKLKQAQTEADIIALLNGEVQFEADFDASLIQLLFPA
SDMIQMSAVAGGLLKNSGNAESAFVAELVTKEPTHLGKGLWLVSTDKGVKRSGMSIVTTANGCEFNELPVKGLIAIASCN
ASHQAFLTTITKAITEQQQESILSATSEQLLAMFSTSTEETTVNADADNTAIFKIKNAHGLHARPGAMLVAEAKKYESKI
TVLNVNGDGNAVNAKSLMKVIALGVKHGHELQFTADGADAKEALAGIGAAIASGLGEG

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG4668

COG function: function code G; Mannitol/fructose-specific phosphotransferase system, IIA domain

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI1788494, Length=381, Percent_Identity=45.4068241469816, Blast_Score=312, Evalue=2e-86,
Organism=Escherichia coli, GI1790027, Length=144, Percent_Identity=36.8055555555556, Blast_Score=105, Evalue=7e-24,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178
- InterPro:   IPR001020
- InterPro:   IPR005698
- InterPro:   IPR000032
- InterPro:   IPR002114 [H]

Pfam domain/function: PF00381 PTS-HPr; PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 39730; Mature: 39730

Theoretical pI: Translated: 5.23; Mature: 5.23

Prosite motif: PS00369 PTS_HPR_HIS ; PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLTKNDITLQQSAADKTTAIKAIAKQLATKGLVAENYVDGMLNREQQNSTFLGNGIAI
CEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCCEEC
PHGTVDTRELVNQTGVAIHHFPQGVNWGDDNVVYVAIGIAAKSDEHLGILKQLTKVLSAD
CCCCCCHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCC
GVEEKLKQAQTEADIIALLNGEVQFEADFDASLIQLLFPASDMIQMSAVAGGLLKNSGNA
CHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
ESAFVAELVTKEPTHLGKGLWLVSTDKGVKRSGMSIVTTANGCEFNELPVKGLIAIASCN
HHHHHHHHHHCCCCHHCCEEEEEECCCCCHHCCCEEEEECCCCCCCCCCCCCEEEEEECC
ASHQAFLTTITKAITEQQQESILSATSEQLLAMFSTSTEETTVNADADNTAIFKIKNAHG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCC
LHARPGAMLVAEAKKYESKITVLNVNGDGNAVNAKSLMKVIALGVKHGHELQFTADGADA
CCCCCCCEEEEEHHHCCCEEEEEEECCCCCEECHHHHHHHHHHHHCCCCEEEEEECCCCH
KEALAGIGAAIASGLGEG
HHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MLTLTKNDITLQQSAADKTTAIKAIAKQLATKGLVAENYVDGMLNREQQNSTFLGNGIAI
CEEEECCCEEEECCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCEEECCCEEC
PHGTVDTRELVNQTGVAIHHFPQGVNWGDDNVVYVAIGIAAKSDEHLGILKQLTKVLSAD
CCCCCCHHHHHHHCCCEEEECCCCCCCCCCCEEEEEEEEEECCCCHHHHHHHHHHHHCCC
GVEEKLKQAQTEADIIALLNGEVQFEADFDASLIQLLFPASDMIQMSAVAGGLLKNSGNA
CHHHHHHHHHCCCCEEEEECCEEEEECCCCHHHHHHHCCCHHHHHHHHHHHHHHCCCCCC
ESAFVAELVTKEPTHLGKGLWLVSTDKGVKRSGMSIVTTANGCEFNELPVKGLIAIASCN
HHHHHHHHHHCCCCHHCCEEEEEECCCCCHHCCCEEEEECCCCCCCCCCCCCEEEEEECC
ASHQAFLTTITKAITEQQQESILSATSEQLLAMFSTSTEETTVNADADNTAIFKIKNAHG
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEECCCCCEEEEEEECCCC
LHARPGAMLVAEAKKYESKITVLNVNGDGNAVNAKSLMKVIALGVKHGHELQFTADGADA
CCCCCCCEEEEEHHHCCCEEEEEEECCCCCEECHHHHHHHHHHHHCCCCEEEEEECCCCH
KEALAGIGAAIASGLGEG
HHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]