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The map label for this gene is luxB [H]

Identifier: 59714103

GI number: 59714103

Start: 1044286

End: 1045266

Strand: Reverse

Name: luxB [H]

Synonym: VF_A0920

Alternate gene names: 59714103

Gene position: 1045266-1044286 (Counterclockwise)

Preceding gene: 59714104

Following gene: 59714102

Centisome position: 78.57

GC content: 33.13

Gene sequence:

>981_bases
ATGAATTTTGGATTATTTTTTCTCAACTTTCAGAAGAATGGGATAACATCGGAGGAAACGTTAAATTGTATGGTTGATAC
TGTCACTTTAATTGATTCTGATGATTATCATTTTGATTCTGCTTTTGTATGTGAACACCACTTTTCTCGAAATGGTATTG
TTGGGGCTCCTATCACTGCTGCAGGCTTTTTACTTGGTTTAACGAACCGATTACGTATTGGCTCATTAAATCAAGTAATA
ACAACTCATCACCCGGTACGAGTTGCGGAAGAGGTTGGCGTGTTGGATCAAATGTCTGAAGGGCGTTTTGTTCTTGGTTT
TAGTGATTGTGAAAATGATTTTGAAATGGAATTTTTTAAGCGTCATATTCCATCTCGTCAGCAACAATTTGAAGCATGTT
ATGACGTTATTAATGAAGCATTAACTACAGGATATTGTCATCCACAGAATGATTTTTATAATTTTCCCAAAATTTCAGTT
CATCCACATTGTTATAGTGAAAATGGCCCTAAGCAATATGTATTAGCTACATCAAAAGAAGTGGTTAAATGGGCTGCGAA
AAAGGCATTATCTCTAATTTTTAAGTGGGATGATAACCTAGAAGTTAAAGAACGTTACGCAGTACTTTATAATAAAACAG
CTCAACAATATGGCGTAGATATTTCAGACGTTGATCATCAATTAACGGTGATTGTGAACTTAAATACTGATAAAAATACG
GCTCAAGAAGAAGTGAGAGAGTATTTAAAAGGCTATATTACTGAAACCTACCCTCATATTAGTAGGGATGAAAAAATTAA
CTGTATTATTGAAGAGAATGCTGTTGGTACTCATAATGACTATTATGAATCGACAAAATTAGCAGTGGAAAAAACAGGGG
CTAAAAATATTTTATTATCCTTTGAGTCAATGAATGATATTAATGATGTAAAAGATATTATTGATATGTTGAATCAGAAA
ATCGAAAAGAATCTGCCTTAA

Upstream 100 bases:

>100_bases
GAGGCTAATGGCACTGAAGAAGAAATTATTGCTTCGATGGAACTCTTCATGACCAAGGTTGCCCCTTTCTTAAAAGAACC
TCAAAAATAAGGAATATATT

Downstream 100 bases:

>100_bases
TAGATTGAGGATAATATTTTAGGTTAAATTGCCATTATTTATCGAGTGTTGTTGACGTTAGCTATTATTAGGATAGATAT
GACTAATCATATTGAATATA

Product: luciferase beta chain LuxB

Products: NA

Alternate protein names: Bacterial luciferase beta chain [H]

Number of amino acids: Translated: 326; Mature: 326

Protein sequence:

>326_residues
MNFGLFFLNFQKNGITSEETLNCMVDTVTLIDSDDYHFDSAFVCEHHFSRNGIVGAPITAAGFLLGLTNRLRIGSLNQVI
TTHHPVRVAEEVGVLDQMSEGRFVLGFSDCENDFEMEFFKRHIPSRQQQFEACYDVINEALTTGYCHPQNDFYNFPKISV
HPHCYSENGPKQYVLATSKEVVKWAAKKALSLIFKWDDNLEVKERYAVLYNKTAQQYGVDISDVDHQLTVIVNLNTDKNT
AQEEVREYLKGYITETYPHISRDEKINCIIEENAVGTHNDYYESTKLAVEKTGAKNILLSFESMNDINDVKDIIDMLNQK
IEKNLP

Sequences:

>Translated_326_residues
MNFGLFFLNFQKNGITSEETLNCMVDTVTLIDSDDYHFDSAFVCEHHFSRNGIVGAPITAAGFLLGLTNRLRIGSLNQVI
TTHHPVRVAEEVGVLDQMSEGRFVLGFSDCENDFEMEFFKRHIPSRQQQFEACYDVINEALTTGYCHPQNDFYNFPKISV
HPHCYSENGPKQYVLATSKEVVKWAAKKALSLIFKWDDNLEVKERYAVLYNKTAQQYGVDISDVDHQLTVIVNLNTDKNT
AQEEVREYLKGYITETYPHISRDEKINCIIEENAVGTHNDYYESTKLAVEKTGAKNILLSFESMNDINDVKDIIDMLNQK
IEKNLP
>Mature_326_residues
MNFGLFFLNFQKNGITSEETLNCMVDTVTLIDSDDYHFDSAFVCEHHFSRNGIVGAPITAAGFLLGLTNRLRIGSLNQVI
TTHHPVRVAEEVGVLDQMSEGRFVLGFSDCENDFEMEFFKRHIPSRQQQFEACYDVINEALTTGYCHPQNDFYNFPKISV
HPHCYSENGPKQYVLATSKEVVKWAAKKALSLIFKWDDNLEVKERYAVLYNKTAQQYGVDISDVDHQLTVIVNLNTDKNT
AQEEVREYLKGYITETYPHISRDEKINCIIEENAVGTHNDYYESTKLAVEKTGAKNILLSFESMNDINDVKDIIDMLNQK
IEKNLP

Specific function: Light-emitting reaction in luminous bacteria. The specific role of the beta subunit is unknown, but it is absolutely required for bioluminescence activity [H]

COG id: COG2141

COG function: function code C; Coenzyme F420-dependent N5,N10-methylene tetrahydromethanopterin reductase and related flavin-dependent oxidoreductases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial luciferase oxidoreductase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018235
- InterPro:   IPR011251
- InterPro:   IPR016048
- InterPro:   IPR002103 [H]

Pfam domain/function: PF00296 Bac_luciferase [H]

EC number: =1.14.14.3 [H]

Molecular weight: Translated: 37306; Mature: 37306

Theoretical pI: Translated: 4.84; Mature: 4.84

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNFGLFFLNFQKNGITSEETLNCMVDTVTLIDSDDYHFDSAFVCEHHFSRNGIVGAPITA
CCCEEEEEEEECCCCCHHHHHHHEEEEEEEEECCCCCCCCEEEEHHHCCCCCCCCCCHHH
AGFLLGLTNRLRIGSLNQVITTHHPVRVAEEVGVLDQMSEGRFVLGFSDCENDFEMEFFK
HHHHHHCCCCEECCCCHHHHHCCCCHHHHHHHCHHHHCCCCCEEEEECCCCCCHHHHHHH
RHIPSRQQQFEACYDVINEALTTGYCHPQNDFYNFPKISVHPHCYSENGPKQYVLATSKE
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECHHH
VVKWAAKKALSLIFKWDDNLEVKERYAVLYNKTAQQYGVDISDVDHQLTVIVNLNTDKNT
HHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCHHHHHCCCHHCCCCEEEEEEEECCCCCH
AQEEVREYLKGYITETYPHISRDEKINCIIEENAVGTHNDYYESTKLAVEKTGAKNILLS
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHEEECCCCCEEEEE
FESMNDINDVKDIIDMLNQKIEKNLP
ECCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNFGLFFLNFQKNGITSEETLNCMVDTVTLIDSDDYHFDSAFVCEHHFSRNGIVGAPITA
CCCEEEEEEEECCCCCHHHHHHHEEEEEEEEECCCCCCCCEEEEHHHCCCCCCCCCCHHH
AGFLLGLTNRLRIGSLNQVITTHHPVRVAEEVGVLDQMSEGRFVLGFSDCENDFEMEFFK
HHHHHHCCCCEECCCCHHHHHCCCCHHHHHHHCHHHHCCCCCEEEEECCCCCCHHHHHHH
RHIPSRQQQFEACYDVINEALTTGYCHPQNDFYNFPKISVHPHCYSENGPKQYVLATSKE
HHCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCEEECCCCCCCCCCCEEEEECHHH
VVKWAAKKALSLIFKWDDNLEVKERYAVLYNKTAQQYGVDISDVDHQLTVIVNLNTDKNT
HHHHHHHHHHEEEEECCCCCCHHHHHHHHHHCHHHHHCCCHHCCCCEEEEEEEECCCCCH
AQEEVREYLKGYITETYPHISRDEKINCIIEENAVGTHNDYYESTKLAVEKTGAKNILLS
HHHHHHHHHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHEEECCCCCEEEEE
FESMNDINDVKDIIDMLNQKIEKNLP
ECCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 3340562 [H]