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The map label for this gene is fre [H]

Identifier: 59714101

GI number: 59714101

Start: 1042357

End: 1043067

Strand: Reverse

Name: fre [H]

Synonym: VF_A0918

Alternate gene names: 59714101

Gene position: 1043067-1042357 (Counterclockwise)

Preceding gene: 59714102

Following gene: 59714099

Centisome position: 78.41

GC content: 31.93

Gene sequence:

>711_bases
ATGAGTATTGATGGGAAAGTTAAGAAAGTGATATTAGCATCAATCAGAAACAATATATACAAAGTGTTTATTACACCAAA
TAATCCGATTGATTTTAAAGCTGGACAATATGTCGTTGTTGATTTTGATAATGAAAAACGACCATTCTCAATTGCGAGTT
GCCCAACAAAAAAAGATGAGATTGAGTTACATGTAGGTGGCTCTGATAGAGAGAAATCATTAGATGCTATTGAGTATTTT
CTTAACGCTTTAGTTGAAGAATCTGTAATAAAGATAGATGTACCATATGGGGATGCTTGGTGGCGTGAAGAAAGCGAAAA
AACGTTGCTATTGATTGCTGAAGGAACAGGATTATCTTACATAAGTAGTATTTTAAAAAATTGCTTAAGTAAAGGGTTCA
CTCAGGATATATATCTTTACTGGGGAGTAAAAGACAGTTCCTTTTTGTATGAAGATGAAGAGTTACTAAATTTATCATTA
AATAATAAAAACTTTCATTATATTCCTGTTATTGAAGATAAAAATGAAGAATGGATAGGGAAAAAAGGCACTGTTCTTGA
TGCTGTTATGGAAGATTTTACTGAACTCACCCATTTTGATATTTATGTTTGTGGGCCTTTCATGATGGCTAAAACTGCAA
AAGAAAAATTAATTGAAGAGAAAAAAGCGAAGTCAGAACAGATGTTTGCCGATGCTTTTGCATACATATAA

Upstream 100 bases:

>100_bases
GGTAACAGTTGAAATTGTTCGACGTTTAAATACACGTGCGCAAAAGGGATGTGCTCTTTCTATGGCAAGTTTTATTCAAA
GTACTATTTAGGGGAGATTT

Downstream 100 bases:

>100_bases
AGAGAATATAAAAAAGCCGGATTATTAATCCGGCTTTTTATTATTTTAACGTGTCTAAAGGTAACCAAGTGACTTCAACT
CCCGCCTGAGAGAACATATC

Product: FMN reductase

Products: NAD(P); FMNH2; 2-octaprenyl-6-hydroxyphenol [C]

Alternate protein names: NA

Number of amino acids: Translated: 236; Mature: 235

Protein sequence:

>236_residues
MSIDGKVKKVILASIRNNIYKVFITPNNPIDFKAGQYVVVDFDNEKRPFSIASCPTKKDEIELHVGGSDREKSLDAIEYF
LNALVEESVIKIDVPYGDAWWREESEKTLLLIAEGTGLSYISSILKNCLSKGFTQDIYLYWGVKDSSFLYEDEELLNLSL
NNKNFHYIPVIEDKNEEWIGKKGTVLDAVMEDFTELTHFDIYVCGPFMMAKTAKEKLIEEKKAKSEQMFADAFAYI

Sequences:

>Translated_236_residues
MSIDGKVKKVILASIRNNIYKVFITPNNPIDFKAGQYVVVDFDNEKRPFSIASCPTKKDEIELHVGGSDREKSLDAIEYF
LNALVEESVIKIDVPYGDAWWREESEKTLLLIAEGTGLSYISSILKNCLSKGFTQDIYLYWGVKDSSFLYEDEELLNLSL
NNKNFHYIPVIEDKNEEWIGKKGTVLDAVMEDFTELTHFDIYVCGPFMMAKTAKEKLIEEKKAKSEQMFADAFAYI
>Mature_235_residues
SIDGKVKKVILASIRNNIYKVFITPNNPIDFKAGQYVVVDFDNEKRPFSIASCPTKKDEIELHVGGSDREKSLDAIEYFL
NALVEESVIKIDVPYGDAWWREESEKTLLLIAEGTGLSYISSILKNCLSKGFTQDIYLYWGVKDSSFLYEDEELLNLSLN
NKNFHYIPVIEDKNEEWIGKKGTVLDAVMEDFTELTHFDIYVCGPFMMAKTAKEKLIEEKKAKSEQMFADAFAYI

Specific function: Probable flavin reductase in the luminescent systems of different marine bacteria [H]

COG id: COG0543

COG function: function code HC; 2-polyprenylphenol hydroxylase and related flavodoxin oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 FAD-binding FR-type domain [H]

Homologues:

Organism=Escherichia coli, GI2367314, Length=224, Percent_Identity=40.1785714285714, Blast_Score=188, Evalue=3e-49,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017927
- InterPro:   IPR001433
- InterPro:   IPR001221
- InterPro:   IPR017938 [H]

Pfam domain/function: PF00175 NAD_binding_1 [H]

EC number: 1.5.1.29 [C]

Molecular weight: Translated: 27018; Mature: 26887

Theoretical pI: Translated: 4.56; Mature: 4.56

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIDGKVKKVILASIRNNIYKVFITPNNPIDFKAGQYVVVDFDNEKRPFSIASCPTKKDE
CCCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCE
IELHVGGSDREKSLDAIEYFLNALVEESVIKIDVPYGDAWWREESEKTLLLIAEGTGLSY
EEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCEEEEEECCCCHHH
ISSILKNCLSKGFTQDIYLYWGVKDSSFLYEDEELLNLSLNNKNFHYIPVIEDKNEEWIG
HHHHHHHHHHCCCCCEEEEEEECCCCCEEECCHHHEEEEECCCCEEEEEEEECCCCHHCC
KKGTVLDAVMEDFTELTHFDIYVCGPFMMAKTAKEKLIEEKKAKSEQMFADAFAYI
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SIDGKVKKVILASIRNNIYKVFITPNNPIDFKAGQYVVVDFDNEKRPFSIASCPTKKDE
CCCHHHHHHHHHHHHCCEEEEEECCCCCCCCCCCCEEEEEECCCCCCEEEECCCCCCCE
IELHVGGSDREKSLDAIEYFLNALVEESVIKIDVPYGDAWWREESEKTLLLIAEGTGLSY
EEEEECCCCCHHHHHHHHHHHHHHHCCCEEEEECCCCCHHCCCCCCCEEEEEECCCCHHH
ISSILKNCLSKGFTQDIYLYWGVKDSSFLYEDEELLNLSLNNKNFHYIPVIEDKNEEWIG
HHHHHHHHHHCCCCCEEEEEEECCCCCEEECCHHHEEEEECCCCEEEEEEEECCCCHHCC
KKGTVLDAVMEDFTELTHFDIYVCGPFMMAKTAKEKLIEEKKAKSEQMFADAFAYI
CCCCHHHHHHHHHHHHHHEEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NAD(P)H2; FMN [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD(P)H2; FMN; 2 2-octaprenylphenol; O2 [C]

Specific reaction: NAD(P)H2 + FMN = NAD(P) + FMNH2 2 2-octaprenylphenol + O2 = 2 2-octaprenyl-6-hydroxyphenol [C]

General reaction: Oxidoreductases [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2254256 [H]