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The map label for this gene is rpoE3 [H]

Identifier: 59714003

GI number: 59714003

Start: 923921

End: 924466

Strand: Reverse

Name: rpoE3 [H]

Synonym: VF_A0820

Alternate gene names: 59714003

Gene position: 924466-923921 (Counterclockwise)

Preceding gene: 59714004

Following gene: 59713998

Centisome position: 69.49

GC content: 32.23

Gene sequence:

>546_bases
ATGAAATTAGAAGTGATTTGGTCCGAGTATCAATTAAGTTTAAAAGCGTTTTTACGCAGTAAAGTGTCTAATCCTGAAGA
TGTTGAAGACTTGCTTCAAGAGATCTTGATTAAAACGCATAAAAACCTGAAAACGATCTCGGATACTAAAAAGGTAAAAT
CATGGATATTTCAAATAGCAAATAATACGATTATTGATTTTTACCGCCAAAATAACAGGCGAACAGAGTTACTAGCAGAT
GATTTATGGTTCGATGAAGAAAATGAAGAGCAAGTAATGAAAGAGCTTTCACGATGTCTTTTGCCTTTTATTGAACAGCT
ATCGAATGAAGATGCGAAATTATTATCAGCTATTGAAATCGAAGGCATATCTCAAAAAGAGTATGCAGAAAAACATGGAT
TAAAATACTCAACGTTAAAATCGAGAGTAAAGAAAAGCCGTGAAAAATTACATGACTTATTTACGAATTGTTGTGATTTC
TCTATAGATGTTCAAGGGAATTTAATCGAATATCAATCTAAAACGGGAAAGTGTAACCGTTGTTAA

Upstream 100 bases:

>100_bases
AGCAACAAATAGTAAACTTTAATCAAGGTAACCAACTATTGGTTACCTTAAGTTGTTTGTACCGCCAAAAAATTAGAAAG
TATAAAGGTAAGTAATTGAT

Downstream 100 bases:

>100_bases
ATGACTGATTCTTTGTACTAAAAAGCCGACATTAATAGTCGGCTTTATTATGTATGTAAGCTATAAATTTTAATTAGGCT
TTAACGAGAACGCTTGCTTC

Product: RNA polymerase sigma factor SigZ

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 181; Mature: 181

Protein sequence:

>181_residues
MKLEVIWSEYQLSLKAFLRSKVSNPEDVEDLLQEILIKTHKNLKTISDTKKVKSWIFQIANNTIIDFYRQNNRRTELLAD
DLWFDEENEEQVMKELSRCLLPFIEQLSNEDAKLLSAIEIEGISQKEYAEKHGLKYSTLKSRVKKSREKLHDLFTNCCDF
SIDVQGNLIEYQSKTGKCNRC

Sequences:

>Translated_181_residues
MKLEVIWSEYQLSLKAFLRSKVSNPEDVEDLLQEILIKTHKNLKTISDTKKVKSWIFQIANNTIIDFYRQNNRRTELLAD
DLWFDEENEEQVMKELSRCLLPFIEQLSNEDAKLLSAIEIEGISQKEYAEKHGLKYSTLKSRVKKSREKLHDLFTNCCDF
SIDVQGNLIEYQSKTGKCNRC
>Mature_181_residues
MKLEVIWSEYQLSLKAFLRSKVSNPEDVEDLLQEILIKTHKNLKTISDTKKVKSWIFQIANNTIIDFYRQNNRRTELLAD
DLWFDEENEEQVMKELSRCLLPFIEQLSNEDAKLLSAIEIEGISQKEYAEKHGLKYSTLKSRVKKSREKLHDLFTNCCDF
SIDVQGNLIEYQSKTGKCNRC

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released [H]

COG id: COG1595

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit, sigma24 homolog

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. ECF subfamily [H]

Homologues:

None

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR014304
- InterPro:   IPR000838
- InterPro:   IPR007627
- InterPro:   IPR013249
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF08281 Sigma70_r4_2 [H]

EC number: NA

Molecular weight: Translated: 21285; Mature: 21285

Theoretical pI: Translated: 6.29; Mature: 6.29

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.8 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.8 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLEVIWSEYQLSLKAFLRSKVSNPEDVEDLLQEILIKTHKNLKTISDTKKVKSWIFQIA
CCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NNTIIDFYRQNNRRTELLADDLWFDEENEEQVMKELSRCLLPFIEQLSNEDAKLLSAIEI
CCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EGISQKEYAEKHGLKYSTLKSRVKKSREKLHDLFTNCCDFSIDVQGNLIEYQSKTGKCNR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEECCCCCCCCC
C
C
>Mature Secondary Structure
MKLEVIWSEYQLSLKAFLRSKVSNPEDVEDLLQEILIKTHKNLKTISDTKKVKSWIFQIA
CCEEEEHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHH
NNTIIDFYRQNNRRTELLADDLWFDEENEEQVMKELSRCLLPFIEQLSNEDAKLLSAIEI
CCHHHHHHHCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
EGISQKEYAEKHGLKYSTLKSRVKKSREKLHDLFTNCCDFSIDVQGNLIEYQSKTGKCNR
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCEECCCCCEEEECCCCCCCCC
C
C

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9308178; 9384377 [H]